Serum samples
Serum samples from naïve animals: Serum samples from clinically healthy and unvaccinated animals, including 310, 175, and 61 samples from swine, cattle, and sheep, respectively, were collected and tested using a liquid-phase blocking ELISA for FMDV O (O-LPBE) and A-LPBE (with negative results; titer, < 1:4). The diagnostic specificity (Dsp) and cut-off value were calculated using these serum samples (Additional file 2: Table S1).
Serum samples from infected animals: The collection of 107 serum samples from swine infected with FMDV A/GDMM/2013 or O/Mya98 at 7–25 days post infection (dpi) was carried out in the Animal Biological Safety Level 3 (ABSL-3) Laboratory at Lanzhou Veterinary Research Institute (Lanzhou, China); 70 serum samples were collected from cattle infected with FMDV (A/GDMM/2013 or O/Mya98) at 8–20 dpi, and 52 serum samples were collected from sheep with clinical symptoms in the field, which tested as NSP positive using two commercial diagnostic kits (3ABC-bELISA and PrioCHECK FMDV NSP ELISA). The diagnostic sensitivity (Dsn) and cut-off value were calculated using these samples. In addition, a total of 32 serum samples collected from four unvaccinated control swine experimentally challenged with FMDV O/Mya98 at 0 and 2–8 dpi were used to compare the early diagnostic performance of the 3B-cCLIA and two commercial diagnostic kits and detect seroconversion to FMDV SPs and NSPs (Additional file 2: Table S1).
Serum samples from vaccinated animals: One hundred serum samples were collected at 21 days post vaccination (dpv) from swine vaccinated with an FMDV O univalent multiple-epitope recombinant vaccine developed by our research group. The Dsp and cut-off value were calculated using these samples. In addition, 120 serum samples were collected from sows vaccinated 3–15 times with commercial O/A divalent inactivated vaccines. Similarly, 129 serum samples were collected from dairy cows vaccinated with commercial O/A divalent inactivated vaccines every four months for a total of 2, 5 or 10 vaccinations. Seventy-seven serum samples were collected from 15 sheep vaccinated 1–3 times with the laboratory-made FMDV A/AF72, O/Mya98/BY/2010, or Asia 1/JS05 univalent inactivated vaccine. These samples were used to evaluate the diagnostic performances of the 3B-cCLIA and two commercial diagnostic kits and verify the false-positive phenomenon in animals vaccinated multiple times (Additional file 2: Table S1).
Serum samples collected in the field: One hundred seventy-three serum samples were collected from field swine suspected of FMDV infection during 2010–2018. These sera were used to compare the coincidence rates between the 3B-cCLIA and two commercial diagnostic kits (Additional file 2: Table S1).
Serum samples from other virus-infected swine: In this study, one serum sample from a classical swine fever virus (CSFV)-infected swine, one serum sample from a Senecavirus A (SVA)-infected swine, one serum sample from a porcine parvovirus (PPV)-infected swine, one serum sample from a porcine reproductive and respiratory syndrome virus (PRRSV)-infected swine, and two serum samples from porcine circovirus type 2 (PCV2)-infected swine were investigated (Additional file 2: Table S1).
Control sera: A serum sample derived from swine infected with FMDV O/Mya98 at 25 dpi served as standard positive serum (P51). The percentage inhibition (PI) rates for the 3ABC-bELISA and PrioCHECK FMDV NSP ELISA were 92% and 93%, respectively. A standard negative serum sample (P734) was taken from a clinically healthy swine that had not been immunized against FMD. The serum was tested with the O-LPBE and A-LPBE (with negative results; titer, < 1:4), 3ABC-bELISA (with a negative result; PI = 1%), and PrioCHECK FMDV NSP ELISA (with a negative result; PI = −7%).
Antigen and antibodies
The 3ABC-coding region of FMDV A/GDMM/2013 mutated at positions 46 aa and 163 aa was cloned into the pProEXHTB plasmid. The expression and purification of the recombinant 3ABC protein has been described elsewhere [13].
MAbs against the 3ABC protein, designated 2G5 and 9E2, were obtained in our laboratory, and their minimally identified epitopes were “92EYIEKA97”, which is located in 3A, and “23EGPYAGPLE31”, which is located in 3B [3]. The mAbs 2G5 and 9E2 were largely produced by injecting hybridomas into the peritoneal cavities of BALB/c mice and purified by affinity protein G column chromatography. Then, the mAbs 2G5 and 9E2 were conjugated with horseradish peroxidase (HRP). Polyclonal antibodies were obtained by inoculating rabbits with the purified 3ABC protein.
Development of a competitive CLIA using a mAb against NSP 3B
Checkerboard titration was performed to optimize the conditions of the 3B-cCLIA. Coating with the mAb 2G5 on 96-well white plates (Costar, catalogue number: 43923) was carried out at 1, 0.5, 0.25, and 0.125 μg/mL concentrations in a 100-μL volume followed by overnight incubation at 4 °C. After washing, purified 3ABC protein was diluted to 0.5, 0.25, and 0.125 μg/mL in PBS containing 0.05% Tween-20 (PBST) and added to each well, and the plate was incubated for 1 h at 37 °C. After three PBST washes, each well received 200 μL of blocking buffer, followed by incubation at 37 °C for 2 h. Then, serial dilutions (1:2.5–1:20) of standard positive serum (P51) and standard negative serum (P734) were carried out with dilution buffer (10% equine serum, 1% casein in PBST), and 50 μL of serum was transferred to each well. Simultaneously, 50 μL of HRP-conjugated 9E2 (9E2-HRP) was added to each well at concentrations of 0.08, 0.04, 0.02, 0.01, 0.005, and 0.0025 μg/mL, followed by incubation of the plate at room temperature. After washing five times, a chemiluminescence (CL) substrate (KEY-BIO, Beijing, China) including 50 μL of solution A (luminol and luminous enhancer) and 50 μL of solution B (peroxide solution) was added. The CL signals were measured with a Varioskan LUX (Thermo Scientific, USA) after 5 min. Determination of the optimum mAb 2G5 concentration, 3ABC protein concentration, serum dilution, 9E2-HRP concentration, and incubation time was carried out based on the ratios of the CL values of standard negative serum to those of standard positive serum (N/P).
The 3B-cCLIA was carried out under optimal conditions. The following formula was used to calculate the PI:
$${\text{PI}}\% = \left( {1 - {\text{CLs/CLn}}} \right) \times 100\% ,$$
where the mean CL value of the standard negative serum control is represented by CLn, while the CL values of the test samples are represented by CLs.
A mean CLn ≥ 6,000,000 and mean PI of the standard positive control > 80% indicated assay validity.
Similarly, the 3A + 3B-cCLIA was developed; detailed information is presented in Additional file 1.
Cut-off value, Dsn, and Dsp
The cut-off value of the 3B-cCLIA was determined by testing 875 serum samples from different origins. NSP-negative sera from swine (n = 410), cattle (n = 175), and sheep (n = 61) were used to estimate the Dsp for each species, and NSP-positive sera from swine (n = 107), cattle (n = 70), and sheep (n = 52) were used to estimate the Dsn for each species using MedCalc software.
Comparison of accuracy rates and diagnostic performances
The accuracy rates of two commercial diagnostic kits were also evaluated using the abovementioned 875 NSP-negative and NSP-positive serum samples. The diagnostic performances of the 3B-cCLIA, 3A + 3B-cCLIA and two commercial diagnostic kits were compared by testing 430 serum samples, including 120, 60, and 77 samples from vaccinated swine, cattle, and sheep, respectively, and 173 samples from the field. In addition, serum samples from four swine challenged with FMDV O/Mya98 collected at different times were utilized to evaluate and compare the early diagnostic performances of the four assays.
Estimation of the repeatability and stability of the 3B-cCLIA
To calculate the intra- and interbatch repeatability performances, three replicates of seven serum samples with varying PI values were evaluated on various days using plates coated in the same and different batches. To determine the shelf life of the coated plates, they were vacuum packed and stored at 37 °C for 15 days or at 4 °C for 1 year after blocking.