Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for the rapid detection of antibodies induced by FMDV infection

Table 1 Comparison of 3B-cCLIA with 3A + 3B-cCLIA, 3ABC-bELISA and PrioCHECK FMDV NSP ELISA for the detection of NSP antibodies in sera from naïve, vaccinated and infected animals

Sample source	Total no. samples	3B-cCLIA			3A + 3B-cCLIA			3ABC-bELISA			PrioCHECK® NSP
Sample source	Total no. samples	P^a	N^b	Accuracy rate	P^a	N^b	Accuracy rate	P^a	N^b	Accuracy rate	P^a	N^b	Accuracy rate
Naïve swine	310	1	309	99.68%	1	309	99.68%	2	308	99.35%	0	310	100.00%
Vaccinated swine with multiple-epitope recombinant vaccine	100	1	99	99.00%	1	99	99.00%	1	99	99.00%	0	100	100.00%
Infected swine	107	104	3	97.20%	105	2	98.13%	102	5	95.33%	91	16	85.05%
Naïve bovine	175	1	174	99.43%	1	174	99.43%	2	173	98.86%	0	175	100.00%
Infected bovine	70	67	3	95.71%	67	3	95.71%	66	4	94.29%	63	7	90.00%
Naïve sheep	61	1	60	98.36%	1	60	98.36%	2	59	96.72%	0	61	100.00%
Infected sheep	52	50	2	96.15%	50	2	96.15%	51	1	98.08%	48	4	92.31%
Vaccinated sows with commercial O/A divalent vaccine (3–15 times)	120	1	119	99.17%	1	119	99.17%	3	117	97.50%	0	120	100.00%
Vaccinated dairy cows with commercial O/A divalent vaccine (2–10 times)	129	12	117	90.70%	13	116	89.92%	19	110	85.27%	15	114	88.37%
Vaccinated sheep with O, A or Asia 1 univalent inactivated vaccine (1–3 times)	77	5	72	93.51%	4	73	94.81%	6	71	92.21%	2	75	97.40%
Swine in the filed	173	13	160		14 (13)	159 (159)	99.42%^c	15 (11)	158 (156)	96.53% ^c	9 (7)	164 (158)	95.37% ^c

^aPositive
^bNegative
^cThe coincidence rate of PI value of 3B-cCLIA with those of 3A + 3B-cCLIA, 3ABC-bELISA, and PrioCHECK FMDV NSP ELISA in testing field sera collected from swine was 99.42% (172/173), 96.53% (167/173), and 95.37% (165/173), respectively

ISSN: 1743-422X