Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for the rapid detection of antibodies induced by FMDV infection

Table 2 Antibody to FMDV NSP in sera from cattle and sheep collected following repeated vaccination

Sample source	Total no. samples	3B-cCLIA			3A + 3B-cCLIA			3ABC-bELISA			PrioCHECK® NSP
Sample source	Total no. samples	P^a	N^b	False-positive rate	P^a	N^b	False-positive rate	P^a	N^b	False-positive rate	P^a	N^b	False-positive rate (%)
Vaccinated dairy cows with commercial O/A divalent vaccine (2 times)	30	1	29	3.33%	1	29	3.33%	1	29	3.33%	1	29	3.33
Vaccinated dairy cows with commercial O/A divalent vaccine (5 times)	33	2	31	6.06*	2	31	6.06*	3	30	9.09%	3	30	9.09%
Vaccinated dairy cows with commercial O/A divalent vaccine (10 times)	66	9	57	13.64%	10	56	15.15%	15	51	22.73%	11	55	16.67%
Vaccinated sheep with O, A or Asia 1 univalent inactivated vaccine (1 time)	29	0	29	0%	1	28	3.45%	0	29	0%	0	29	0%
Vaccinated sheep with O, A or Asia 1 univalent inactivated vaccine (2 times)	30	2	28	6.67%	1	29	3.33%	1	29	3.33%	0	30	0%
Vaccinated sheep with O, A or Asia 1 univalent inactivated vaccine (3 times)	18	3	15	16.67%	2	16	11.11%	5	13	27.78%	2	16	11.11%

ISSN: 1743-422X