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Fig. 2 | Virology Journal

Fig. 2

From: Development of a competitive chemiluminescence immunoassay using a monoclonal antibody recognizing 3B of foot-and-mouth disease virus for the rapid detection of antibodies induced by FMDV infection

Fig. 2

Early production of antibodies against FMDV SPs and NSPs detected in sera from experimentally challenged swine. a Thirty-two serum samples from four unvaccinated control swine after challenge with FMDV O/Mya98 were collected at 0 dpi and 2–8 dpi and tested using O-LPBE and ID Screen® FMD Type O Competition to detect anti-SP antibodies. The dashed lines – – – – – and …… represent the cut-off values of O-LPBE (reciprocal log 10 ≥ 1.65 was considered positive) and ID Screen® FMD Type O Competition (S/N ≤ 50% was considered positive), respectively. b The above thirty-two serum samples were also tested using the 3B-cCLIA, the 3A + 3B-cCLIA, and two commercial diagnostic kits (3ABC-bELISA and PrioCHECK FMDV NSP ELISA) to detect anti-NSP antibodies. The dashed line (– – – – –) represents the cut-off value of the 3B-cCLIA, 3ABC-bELISA, and PrioCHECK FMDV NSP ELISA (PI ≥ 50 was considered positive), and the dashed line (……) represents the cut-off value of the 3A + 3B-cCLIA (PI ≥ 40 was considered positive). The bar represents the range of values obtained by detecting sera from four swine at the given time

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Virology Journal

ISSN: 1743-422X