Identification and differential expression analysis of MicroRNAs encoded by Tiger Frog Virus in cross-species infection in vitro
© Yuan et al. 2016
Received: 17 November 2015
Accepted: 19 April 2016
Published: 30 April 2016
Tiger frog virus (TFV), dsDNA virus of the genus Ranavirus and family Iridoviridae, causes a high mortality of tiger frog tadpoles cultured in Southern China. MicroRNAs (miRNAs) have been identified in many viruses especially DNA viruses such as Singapore Grouper Iridoviruses (SGIV). MicroRNAs play important roles in regulating gene expression for virus subsistence in host. Considering that TFV infects cells of different species under laboratory conditions, we aim to identify the specific and essential miRNAs expressed in ZF4 and HepG2 cells.
We identified and predicted novel viral miRNAs in TFV-infected ZF4 and HepG2 cells by deep sequencing and software prediction. Then, we verified and described the expression patterns of TFV-encoded miRNAs by using qRT-PCR and Northern blot.
Deep sequencing predicted 24 novel TFV-encoded miRNAs, and qRT-PCR verified 19 and 23 miRNAs in TFV-infected ZF4 (Group Z) and HepG2 (Group H) cells, respectively. Northern blot was performed to validate eight and five TFV-encoded miRNAs in Groups H and Z, respectively. We compared the expression of TFV-encoded miRNAs from two groups and defined TFV-miR-11 as the essential viral miRNA and TFV-miR-13 and TFV-miR-14 as the specific miRNAs that contribute to HepG2 cell infection.
We identified novel viral miRNAs and compared their expression in two host cells. The results of this study provide novel insights into the role of viral miRNAs in cross-species infection in vitro.
As first identified in the nematode Caenorhabditis elegans [1, 2], microRNAs (miRNAs) have been found in almost all multicellular eukaryotes . Since let7 from C. elegans was found completely conserved within the genomes of mice and humans , more than 15,000 novel miRNAs have been identified . Mature miRNAs are noncoding RNAs approximately 21 to 22 nucleotides long  that regulate cellular processes such as immunity, apoptosis, development and other important aspects, by guiding the RNA-induced silencing complex to complementary mRNAs in 3’ untranslated regions (3’ UTRs) .
The first virus-encoded miRNAs were identified from Epstein–Barr virus (EBV) in 2004 . Since then, several virus-encoded miRNAs have been found in different DNA viruses , including members of the Herpesviridae, Polyomaviridae, Ascoviridae, Baculoviridae, Iridoviridae and Adenoviridae families , and in a lone RNA virus, bovine leukemia virus . The herpesviruses contribute the most virus-encoded miRNAs thus far and have been the focus of several studies on miRNA function . Some virus-encoded miRNAs mimic host miRNAs and regulate the host transcripts to help the virus stay in the host . For example, miR-BART5 encoded by EBV inhibits the protein p53 upregulated modulator of apoptosis (PUMA) to prevent cell apoptosis, which facilitates the establishment of latent infection . Viral miRNAs also limit the lytic cycle; for instance, Kaposi’s sarcoma-associated herpesvirus (KSHV)-encoded miR-K12-1 targets the IκBα transcript 3’ UTR to reduce the expression and thus rescue the NF-κB activity inhibited by IκBα and delay viral lytic replication . Virus-encoded miRNAs not only target cellular genes but also regulate viral genes. The KSHV miRNAs miR-K12-9 and miR-7-5p inhibit the expression of the replication and transcription activator (RTA), which is the master switch of the latent–lytic cycle [16, 17]. In Simian vacuolating virus 40, virus-encoded miRNAs cleave the early viral mRNAs to reduce the viral T-antigen expression at the late stages of infection and evade the immune response . These studies show that virus-encoded miRNAs play vital roles during virus life cycle, which includes infection, replication, and release.
Iridoviruses are icosahedral cytoplasmic viruses that have double-stranded DNA genomes with terminal redundancy and circular permutation . According to the Ninth Report of the International Committee on Taxonomy of Viruses, the Iridoviridae family consists of five genera: Chloriridovirus, Iridovirus, Lymphocystivirus, Megalocytivirus and Ranavirus . Tiger Frog Virus (TFV) is the causative pathogen of abdominal distension disease, which causes a high mortality of tiger frog (Rana tigrina rugulosa) tadpoles cultured in Southern China . TFV has been isolated from diseased tadpoles of tiger frog and classified as a member of the genus Ranavirus via complete genome sequencing . Computational analysis revealed that the deduced TFV gene products exhibit more than 90 % similarity to those of frog virus 3 (FV3), the type species of Ranavirus . As a member of Ranavirus, TFV can infect different cells, including fish cells, fathead minnow (FHM) cells, zebrafish embryonic fibroblast (ZF4) cells , and mammalian HepG2 cells , under laboratory conditions. The miRNAs encoded by iridoviruses were first identified using stem-loop quantitative real-time PCR (qRT-PCR) in Singapore grouper iridovirus (SGIV) . In the present study, we identified novel viral miRNAs encoded by TFV and analyzed the diversity of miRNA expression in fish (ZF4) and mammalian (HepG2) cells to define the essential and specific miRNAs in cross-species in vitro.
Cells and virus
FHM cells were cultured in Medium 199 (Gibco, Life Technologies, USA) supplemented with 10 % fetal bovine serum (FBS) (Biochrom, Merck Millipore, Darmstadt, Germany) at 27 °C. ZF4 cells were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (Gibco, Life Technologies, USA) supplemented with 10 % FBS at 27 °C. HepG2 cells were cultured in complete Dulbecco’s modified Eagle’s medium (Gibco, Life Technologies, USA) supplemented with 10 % FBS at 37 °C in a 2.5 % CO2-humidified chamber .
TFV was originally isolated from diseased tiger frog tadpoles from Nanhai (Guangdong, China) and is being maintained in our laboratory . FHM cells were used as the host cells to propagate TFV at 27 °C, and the virus was harvested 3 days after the cytopathic effect was marked. Virus suspension was produced by freeze–thaw the virus–cell mixture three times, followed by filtration with a 0.45 μm Millex-HV Filter (Merck Millipore, Darmstadt, Germany). The virus titer was determined through the 50 % endpoint method using the 50 % tissue culture infective dose (TCID50), and a multiplicity of infection value of 10 was used for infection [28, 29].
Sampling and RNA isolation
ZF4 and HepG2 cells were inoculated with TFV and incubated at 27 °C for 1 h. After infection, the inoculum was removed, and the two types of cells were cultured in their respective medium. Infected ZF4 cells were collected at 4, 12, 24, 48, and 72 h postinfection, whereas infected HepG2 cells were collected without medium at 4, 12, 24, 48, 72, 96, and 120 h postinfection. The two samples were then pooled for RNA isolation. Total RNA was extracted using TRI Reagent® (Sigma, USA) following the technical bulletin, quantified using an ND-1000 Nanodrop® Spectrophotometer (Thermo Scientific, USA), and then verified for quality using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Only samples with an RNA integrity number greater than nine were used for sequencing.
Small RNA library construction and Solexa sequencing
For small RNA library construction, approximately 20 μg of total RNA was size-fractionated on a denaturing polyacrylamide gel (PAGE), and small RNAs (18–30 nt) were eluted from the excised gel slice. Proprietary adaptors were then ligated to the 5’ and 3’ termini of the RNA to allow reverse transcription and PCR amplification. The generated cDNA library was used for Solexa sequencing in an Illumina Genome Analyzer (Illumina, USA) in accordance with the manufacturer’s instructions [30, 31].
Sequencing data and annotation analysis
Sequencing data were cleaned by removing adaptor sequences, low-quality tags, and contaminants to obtain clean reads and assess the length distribution of small RNAs. After exploring small RNA distribution across the zebrafish (Danio rerio) and human (Homo sapiens) genomes, rRNA, tRNA, snRNA, snoRNA, and other noncoding RNAs were identified by alignment using the Rfam 10.1 (http://rfam.xfam.org/) and GenBank (http://www.ncbi.nlm.nih.gov/genbank/) databases; known miRNAs were identified by alignment using miRBase (Version 19.0) (http://www.mirbase.org/); and repeat-associated small RNAs and degradation fragments of mRNAs were identified.
Novel miRNA identification
After annotation analysis, the unannotated sequences were aligned against the TFV genome (GenBank: AF389451.1) to search perfectly matched sequences for novel miRNA prediction. The 100 nucleotides flanking each side of the selected sequences were used to predict novel miRNA candidates by analyzing hairpin structures and calculating secondary structures with Mireap (http://sourceforge.net/projects/mireap/) . Only sequences conserved within the stem loop that folded into hairpins were considered putative novel miRNAs.
The infected cells were harvested, and total RNA was extracted using TRI Reagent® as described above. miRNA qRT-PCR primers specific for novel miRNAs and human U6 were designed by RiboBio (Guangzhou, China) and described as a bulge-loop™ miRNA qRT-PCR primer set (one reverse transcription primer and a pair of qRT-PCR primers in every set) . The zebrafish U6 primers for signal normalization were designed as previously described . Reverse transcription and RT-PCR were performed in LightCycler 480 (Roche, Mannheim, Germany) in accordance with the manufacturer’s instructions.
Northern blot was performed as previously described with slight modifications . In brief, 20 μg of total RNA extracted from infected cells was separated on a 15 % denaturing PAGE (7.5 M urea). The sample was transferred to positively charged nylon membranes (Roche, Mannheim, Germany) and cross-linked to the membranes through UV irradiation (120 mJ/cm2) . After pre-hybridization in Ultrasensitive Hybridization Buffer (Ambion, Life technologies, USA) for more than 0.5 h at 37 °C, blots were hybridized overnight at 37 °C with 0.5 nM miRCURY LNA™ probes modified both by 5’ digoxigenin (DIG) and 3’ DIG (Exiqon, Vedbaek, Denmark). Afterward, the blots were sequentially washed using low-stringency, high-stringency, and washing buffers at 37 °C and then incubated in blocking buffer (Roche, Mannheim, Germany) for 3 h. The blots were subsequently incubated in blocking buffer with an anti-DIG-AP Fab fragment solution (Roche, Mannheim, Germany) for 0.5 h and then washed in DIG washing buffer (Roche, Mannheim, Germany). The blots were visualized through autoradiography using a CDP-Star chemiluminescent substrate for alkaline phosphatase (Roche, Mannheim, Germany).
Transfection of the TFV miR-11 inhibitor
micrOFF™ inhibitor of TFV miR-11 and micrOFF™ inhibitor Negative Control #24 were purchased from RiboBio (Guangzhou, China). Lipofectamine® 2000 Transfection Reagent (Invitrogen, ThermoFisher Scientific, USA) was used for transfection to HepG2 in accordance with the standard protocol. In brief, the cells were seeded until 30 % confluence at transfection. The inhibitor (25 pmol) and Lipofectamine® 2000 Transfection Reagent (1 μL) were diluted in 50 μL of Opti-MEM® I Reduced Serum Medium (Invitrogen, ThermoFisher Scientific, USA) and then incubated for 5 min at room temperature. The inhibitor and Lipofectamine® 2000 Transfection Reagent were mixed and then incubated for 20 min at room temperature. The mixture was transferred to a 400 μL culture medium. After incubation at 37 °C for 4 h, the transfection mixture was replaced with fresh medium with 10 % serum and then infected with TFV.
Absolute qRT-PCR of genomic DNA
The supernatant and the supernatant–cell mixture were harvested, and total genomic DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN, USA). Absolute qRT-PCR was performed using primers for the TFV Major Caspd Protein (MCP) gene (forward primer: 5’-TCGCTGGTGGAGCCCTGGTA-3’, reverse primer: 5’-GGCGTTGGTCAGTCTGCCGTA-3’). This primer pair was used to amplify a region from 97034 to 97163 of the TFV genome. The plasmid pCMV-myc-TFV MCP, which served as the internal standard, was serially diluted by 10-fold to generate a standard curve of absolute qRT-PCR. The PCR reaction contained 5 μL of 2 × SYBR® Premix Ex Taq™ (TaKaRa, China), 1 μL of DNA template, 0.2 μL of 10 μM primers, and 3.6 μL of H2O. The absolute qPCR conditions were as follows: one cycle of 95 °C for 20 s and 40 cycles of 10 s at 95 °C, 20 s at 60 °C, and 1 s at 72 °C. Absolute qRT-PCR was performed at three replicates per sample.
This study was performed in strict accordance with the recommendations in the Guide for the Institutional Animal Care and Use Commission (IACUC). The protocol was approved by the Committee on the Ethics of Animal Experiments of the Sun Yat-sen University. All surgery was performed under MS-222 anesthesia, and every effort was made to minimize suffering.
Deep sequencing of small RNAs from ZF4 and HepG2 cells infected by TFV
Distribution of small RNAs from TFV-infected HepG2 and ZF4 cells
Prediction of novel TFV-encoded miRNAs
Small RNAs matched to TFV genome
miRNA matched to TFV genome
Names and sequences of TFV-encoded miRNAs
5’- GTTGCGGTGCCTGGGGTCGGGA -3’
5’- AGACGGTCGGGCTTGACGTTGAG -3’
5’- GCGGGCACAGGGTGGTGACTTT -3’
5’- GCTGGTGTAGATGGTGCCTGTCC -3’
5’- AGACAGCTGCCTGACGTGAAA -3’
5’- AGGCGGTGGCGTCGTAGGTCTTG -3’
5’- GGACCTGCCGTCGGGGACGTG -3’
5’- AGTCTCGGGCCTGAAGGGAAGGAC -3’
5’- GTAGGCCTTGACGCACCTGACGTG -3’
5’- AGGCTGATGTCCATGTCGTCG -3’
5’- TGAGGGTGACCTGACAGGGCTGT -3’
5’- GAGCCAAGTCTGCCTGTCGTCGGC -3’
5’- GGACGTCCCCGCGCCCGTGCTG -3’
5’- CCTGCACACGGGGTCGGACGCCA -3’
5’- GAGTCTCTCTTGTTGGTCTTTGG -3’
5’- CCCACTGGACGGGCTCGGACGGCC -3’
5’- CAACGGGACTGTGGGGTTGAG -3’
5’- GCTCAGGGCGGTGGAGTAGGT -3’
5’- TGTGCGCCCAGGAGCTGTCTGT -3’
5’- CCCACGGCGGGGGGGACAATGT -3’
5’- AGAGATTGGTCCTGAAGAGTTT -3’
5’- AGACGTTGGCCTCGACGGTGTCGG -3’
5’- TCTCGGGGTCGATGGAATTTG -3’
5’- GGGTCCGCGTACAGCAGCGACG -3’
Localization of TFV-encoded miRNAs in genome
Verification of novel TFV-encoded miRNAs by bulge-loop qRT-PCR
In both groups, miRNA expression increased with the period of infection. In Group H, TFV-miR-4, -10, -12, -13, and -14 were detected at 4 h postinfection (hpi); TFV-miR-01, -08, and -09 were detected at 24 hpi; TFV-miR-07, -11, -15, -17, -18, -19, and -20 started at 48 hpi; and TFV-miR-05, -22, and -24 were expressed last at 72 hpi. For Group Z, no miRNAs were detected at 4 hpi; the earliest miRNAs appeared at 24 hpi, including TFV-miR-09, -10, -11, -12, -14, -17, -18, -19, and -20; TFV-miR-01, -04, -05, -06, -07, -08, -13, -15, -16, -21, -22, and -24 were detected at 48 hpi; and TFV-miR-2 and -3 appeared at 72 hpi (Fig. 4b, Additional file 2: Figure S2). The amplification curves of some typical miRNAs between the highest expression and negative control (uninfected cells) are shown in Fig. 4c. These results verified existing miRNAs and showed that viral miRNAs were expressed earlier in Group H, i.e., the infected mammalian cells, than in Group Z.
Northern blot of TFV-encoded miRNAs
Comparison of expression profiles of TFV-encoded miRNAs in infected HepG2 and ZF4 cells
We also compared the expression levels of TFV miR-08, -11, and -22 by performing Northern blot, with 18S RNA as the control. Consistent with the results of qRT-PCR, TFV-miR-08 and -22 had higher expression levels in Group Z, whereas TFV-miR-11 had a higher expression level in Group H (Fig. 5c). The difference in expression levels of the TFV-encoded miRNAs from the two groups might provide a clue to elucidate the mechanism by which the virus adapts itself to different types of host cells.
TFV miR-11 antagomir inhibits the production and induces the release of TFV virions
TFV miR-11 is the most abundant miRNA among all the TFV encoded miRNAs in both Groups H and Z. We transfected the TFV miR-11 inhibitor by binding to mature miRNAs into HepG2 cells following TFV infection to test the role of TFV miR-11 in the life cycle of TFV. We detected the amount of virions from the supernatant and the supernatant–cell mixture through absolute qRT-PCR of TFV MCP. In the series of infection time points, viral genomic DNA was detected at 12 hpi and significantly increased at 48 hpi in the supernatant–cell mixture. Less viral genomic DNA was detected in the TFV miR-11 inhibitor-transfected cells at every time point (Fig. 6b). A large amount of viral genomic DNA was detected at 96 hpi in the supernatant, and much more viral genomic DNA was detected after transfection with the TFV miR-11 inhibitor (Fig. 6b). At 120 hpi, the copy numbers of viral genomic DNA in the supernatant and the mixture were almost the same. This result indicates that almost all the virions were released from the hosts after transfection with TFV miR-11. This result indicates that TFV miR-11 induces virion release from host cells.
Discussion and Conclusions
Several studies have identified miRNAs encoded by DNA viruses, but little is known about the roles of viral miRNAs in cross-species infection. As a member of the genus Ranavirus , TFV can infect a wide range of host cells under laboratory conditions, similar to the type species FV3; thus, TFV can be used as a model to investigate the functions of miRNAs in different types of host cells [24, 25, 39]. In the present study, we selected HepG2 and ZF4 cells as infection models to investigate the distribution of miRNA expression in cross-species infection. After deep sequencing small RNAs, we obtained 22,030,626 and 25,952,342 high-quality reads from infected HepG2 and ZF4 cells, respectively, and predicted 24 novel viral miRNAs. We then identified 23 novel TFV-encoded miRNAs through qRT-PCR and validated 10 of them through Northern blot. This study is the first to report the diversity of miRNA expression in TFV-infected HepG2 and ZF4 cells and define TFV-miR-11 as the essential viral miRNA and TFV-miR-13 and -14 as the specific viral miRNAs for HepG2 infection. We transfected the TFV miR-11 inhibitor before infection and found that this inhibitor induce the release of virions. Significant differences in miRNA expression were found between HepG2 and ZF4 cells, indicating new mechanisms of cross-species infection.
The patterns of viral miRNA location in the genome significantly varied among different types of viruses, which provides clues to the function of viral miRNAs . The 12 miRNAs encoded by KSHV, a well-known oncogenic human herpesvirus, were located as a cluster in the region of ORF K12 mRNA to the start codon of ORF71 [40, 41]. These 12 miRNAs are highly expressed in latently infected B cells, miR-K12 is located in ORF K12, miR-K10 is located in the 3’ UTR of K12, and the 10 other miRNAs are all in one intron, which is reminiscent of the function of latent infection by KSHV-encoded miRNAs . For the first viral miRNAs identified in iridovirus, the locations of SGIV-encoded miRNAs are completely different. The SGIV-encoded miRNAs are scattered throughout the entire genome, situated both on and in between the ORFs. This type of miRNA may not only focus on one virus gene but also on a number of genes from both the virus and the host. Similar to SGIV-encoded miRNAs, TFV-encoded miRNAs are located throughout the entire genome. Those that are antisense to the ones located within ORF and in the 3’ downstream sequence of the neighboring ORFs may inhibit the corresponding viral genes. Others such as TFV-miR-01 located in the intragenic region between the 5’ upstream sequences of ORF003L and ORF004R may focus on host mRNAs.
The aim of our future work is to reveal the functions of TFV-encoded miRNAs. To date, the functions of virus-encoded miRNA can be classified into two groups: one is to mimic the host miRNA by sharing the same seed region to modulate the target mRNA, and the other is to target viral genes. As mentioned above, the localization of TFV-encoded miRNAs in the genome revealed the potential function specific to the viral genes. The regulation of viral protein expression by the miRNA encoded by itself slightly differ from the common pattern . Aside from targeting the 3’ UTR of the viral transcripts [16, 17], virus-encoded miRNAs can also bind viral mRNAs perfectly matched for degradation [18, 43], and the target binding site is also located within the 5’ UTR of the mRNA . In the present study, we characterized the localization of each miRNA in the genome and found that TFV-miR-22 was located in TFV ORF096R and antisense to the transcript. Previous reports have verified ORF096R as the major capsid protein , which is the basic structural protein of the virus. Another miRNA that drew our interest is TFV-miR-08, which was detected by Northern blot in both groups, located in the opposite strand of the 3’ downstream sequence of TFV ORF080L. ORF080L is a putative membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (LITAF), a transcription factor affecting TFV-α expression  and regulating inflammatory cytokines . Recent investigations have revealed that LITAF colocalizes with FV3 ORF75L , which is highly similar to TFV ORF080L, and that SGIV-miR-13 is located in SGIV ORF136R, which has been designated as LITAF . Although the 3’ UTR of TFV ORF096R has not been identified, recent investigations on LITAF have motivated us to seek the relationship between TFV-miR-08 and LITAF in terms of virus pathogenesis. Interestingly, TFV-miR-18 shares the same seed as human miR-1266. Although its seed region is the same as human miRNA and it can be detected in Group H by Northern blot, TFV-miR-18 may be capable of regulating human genes that may be key for TFV infecting mammalian cells.
Similar to FV3, TFV has a wide range of hosts under laboratory conditions, and its pathogenesis in cross-species infections is intriguing . In addition, the miRNA expression pattern in different host cells provides novel insights into the pathogenesis of TFV. The qRT-PCR results described a complete view of all the miRNAs, showing that TFV-miR-11 is the most abundant among the miRNAs. The diversity of TFV-miR-08, -11, and -22 correlated with the Northern blot result that TFV-miR-08 and -22 had higher expression levels in Group Z and TFV-miR-11 had a higher expression level in Group H. Considering that TFV-miR-11 had the highest expression levels in both groups and can be detected by both methods, we conclude that TFV-miR-11 is the essential viral miRNA for TFV life cycle and that TFV-miR-08 and -22 are important in maintaining TFV in different host cells. Moreover, TFV-miR-13 and -14 levels significantly increased in group H and were only detected in group H by Northern blot. This result suggests that these two miRNAs have specific roles in the TFV infection of mammalian cells. However, the mechanism and function of these miRNAs warrant further investigation.
This work was supported by the National Natural Science Foundation of China (Nos. 31322056, 31370048, and 31330080), the Guangdong Natural Science Foundation (Nos. S2013010012161), the Pearl River S&T Nova Program of Guangzhou (No. 2014 J2200055), the Tip-top Scientific and Technical Innovative Youth Talents of Guangdong special support program (No. 2014TQ01N303), the Foundation for Young Teacher (No. 20130171220009), and the Fundamental Research Funds for the Central Universities (Nos. 151gzd04 and 15lgjc05).
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