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Fig. 6 | Virology Journal

Fig. 6

From: Identification and differential expression analysis of MicroRNAs encoded by Tiger Frog Virus in cross-species infection in vitro

Fig. 6

a Difference in the expression of TFV-encoded miRNAs between infected HepG2 (Group H) and ZF4 (Group Z) cells. As the most abundant miRNA, TFV-miR-11 was taken as the 100-value, with the expression levels of all the other novel miRNAs calculated relative to it. Most of the miRNAs had higher expression levels in Group Z, except for TFV-miR-13 and -14, which were more highly expressed in Group H. b Role of TFV miR-11 in TFV life cycle. Absolute qRT-PCR was applied to detect the copy number of virions by amplifying MCP. The TFV miR-11 inhibitor and negative control were transfected HepG2 cells following TFV infection. The samples were harvested at 12, 24, 48, 72, 96, and 120 h post-infection. Mixture: mixture of cells and supernatant; supernatant: supernatant from infected cells

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