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Fig. 4 | Virology Journal

Fig. 4

From: Identification and differential expression analysis of MicroRNAs encoded by Tiger Frog Virus in cross-species infection in vitro

Fig. 4

Verification of TFV-encoded miRNAs from infected HepG2 and ZF4 cells by bulge-loop qRT-PCR. a Expression profiles of miRNAs from HepG2 cells infected by TFV. TFV-miR-01, -04, -05, -07, -08, -09, -10, -11, -12, -13, -14, -15, -17, -18, -19, -20, -21, -22, and -24 were detected in Group H. b Expression profiles of miRNAs from ZF4 cells infected by TFV. TFV-miR-01, -02, -03, -04, -05, -06, -07, -08, -09, -10, -11, -12, -13, -14, -15, -16, -17, -18, -19, -20, -21, -22, and -24 were detected in Group Z. a, b Expression of miRNAs increased with the period of infection. c Amplification curves of qRT-PCR. Compared with the uninfected cells, the typical miRNAs (TFV-miR-01, -11, and -17) detected by qRT-PCR show notable amplification curves. The black arrow indicate the curve of miRNAs, the black arrow head indicate the curve of negative control that is from uninfected cells

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