- Open Access
A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics
© Wolf et al; licensee BioMed Central Ltd. 2009
- Received: 3 July 2009
- Accepted: 31 July 2009
- Published: 31 July 2009
Nipah virus (NiV) and Hendra virus (HeV) are the only paramyxoviruses requiring Biosafety Level 4 (BSL-4) containment. Thus, study of henipavirus entry at less than BSL-4 conditions necessitates the use of cell-cell fusion or pseudotyped reporter virus assays. Yet, these surrogate assays may not fully emulate the biological properties unique to the virus being studied. Thus, we developed a henipaviral entry assay based on a β-lactamase-Nipah Matrix (βla-M) fusion protein. We first codon-optimized the bacterial βla and the NiV-M genes to ensure efficient expression in mammalian cells. The βla-M construct was able to bud and form virus-like particles (VLPs) that morphologically resembled paramyxoviruses. βla-M efficiently incorporated both NiV and HeV fusion and attachment glycoproteins. Entry of these VLPs was detected by cytosolic delivery of βla-M, resulting in enzymatic and fluorescent conversion of the pre-loaded CCF2-AM substrate. Soluble henipavirus receptors (ephrinB2) or antibodies against the F and/or G proteins blocked VLP entry. Additionally, a Y105W mutation engineered into the catalytic site of βla increased the sensitivity of our βla-M based infection assays by 2-fold. In toto, these methods will provide a more biologically relevant assay for studying henipavirus entry at less than BSL-4 conditions.
- Envelope Protein
- Nipah Virus
- Surrogate Assay
- Hendra Virus
- Entry Characteristic
The henipaviruses, Nipah (NiV) and Hendra (HeV), are emerging zoonoses; the former caused multiple outbreaks of fatal encephalitis in Malaysia, Bangladesh, and India with mortalities ranging from 40-70% while the latter produced respiratory syndromes among thoroughbred horses in Australia whilst also being implicated in the death of a horse handler [1–4]. These two paramyxoviruses, both designated Category C priority pathogens by the NIAID Biodefense Research Agenda, require strict Biosafety Level 4 (BSL-4) containment due to their extreme pathogenicity, unverified mode(s) of transmission, and lack of pre- or post-exposure treatments.
BSL-4 containment limits the opportunities for thorough analysis of live henipavirus entry characteristics. Surrogate assays to study henipavirus entry at less than BSL-4 conditions exist, such as cell-cell fusion or VSV-based NiV-envelope pseudotyped reporter assays. These assays have been used to probe envelope receptor interactions and characterize the determinants of fusion with regards to both the fusion (F) and attachment (G) envelope glycoproteins [5–10]. However, cell-cell fusion lacks the geometric and kinetic constraints found in virus-cell fusion while pseudotyped VSV particles physically resemble Rhabdoviridae rather than the pleomorphic Paramyxoviridae. Therefore, neither assay may fully recapitulate the biological properties of native envelope structures of live henipaviruses. Moreover, pseudotype reporter virus assays depend on efficient transcription and translation of a reporter gene after virus entry. Thus, earlier steps in viral entry, such as matrix uncoating, may also not be resolved by either of these assays.
Many viruses form virus-like particles (VLPs) via expression of their matrix alone (e.g. Sendai, HPIV-1, Ebola, HIV, Rabies) or only in combination with envelope proteins (e.g. Simian Virus 5, Measles) [11–19]. Paramyxoviral matrix proteins direct budding of virions from the surface of infected cells and interact with the endodomain of envelope proteins, ultimately assisting in viral assembly[11, 20]. Specifically, NiV matrix (NiV-M) alone, or in combination with its fusion protein (NiV-F) and receptor-binding protein (NiV-G), buds and forms VLPs efficiently[21, 22]. Additionally, matrix may function to recruit the nucleoprotein-encased genome to the budding site[15, 23]. Paramyxoviral matrix proteins perform essential roles in viral release/budding and presumably rely on late domains[20, 24] for these functions; although typical late domain motifs have not been found in certain paramyxoviral M proteins. Thus, NiV matrix-based VLPs will likely better reflect the biological properties of their live-virus counterparts in entry assays. Here, we developed a VLP-based assay that can be used for analyses of henipaviral entry characteristics under BSL-2 conditions. This VLP assay is based on a β-lactamase-Nipah Matrix (βla-M) fusion reporter protein.
β-lactamase (βla) is a commonly used reporter protein whose reporter activity depends on its ability to cleave β-lactam ring-containing fluorescent or colorimetric substrates. For our purposes, CCF2-AM proved useful as a cell-permeant fluorescent substrate engineered to exhibit a shift from green to blue fluorescence upon βla cleavage [26–28]. CCF2-AM cell loading is nearly 100% efficient, practically irreversible (cytoplasmic esterases prevent CCF2 from diffusing out of the cells), and permits loading of a variety of cell types including primary neuron or microvascular endothelial cells, the main targets of NiV infection. Thus, virus-cell fusion of envelope bearing βla-M VLPs should deliver βla-M to the cytosol leading to fluorescent conversion of the pre-loaded CCF2 substrate. The shift from green to blue fluorescence can then be quantified by flow cytometry or quantitative microscopy.
Genetic optimization of both the expression and the intrinsic enzymatic efficiency of the βla-M reporter allowed for sensitive, specific and relatively high-throughput analyses of henipavirus entry in the absence of vaccinia augmentation. Our results suggest that this strategy may be generalized to other viruses where matrix is the primary determinant of budding and virion morphology.
Synthesis of the β-lactamase-Nipah Matrix (βla-M) fusion construct and its incorporation into virus-like particles (VLPs)
Codon-optimized NiV-M and βla-M were equivalently expressed in transfected 293T cells (Fig. 1b). Notably, fusion of codon-optimized βla to wild-type NiV-M (NiV-M WT ) resulted in almost undetectable expression of βla-M under similar transfection conditions (data not shown). To verify incorporation of NiV-M and βla-M into VLPs, we transfected 293T cells with codon-optimized NiV-M or βla-M along with the corresponding codon-optimized NiV-F and NiV-G envelope glycoproteins. After isolating VLPs from the transfected cell supernatants, we verified the presence of NiV-M or βla-M within the lysed VLPs by immunoblotting with NiV-M-specific antibodies (Fig. 1c). Only M-containing VLPs with both NiV-F and NiV-G on their surface will be infectious in our entry assays and these data suggest that fusion of βla to NiV-M did not perturb the ability of NiV-M to form VLPs or incorporate cognate viral envelope glycoproteins. Coexpression of nucleocapsid (N) along with NiV-M or βla-M did not alter the overall production of M-containing VLPs (data not shown), consistent with findings from other groups.
βla-M+NiV-F/G VLPs morphologically, biochemically, and biologically mimic live NiV
Next, we characterized the morphology of the VLPs by imaging the βla-M VLPs via electron microscopy. Fig. 2b shows that βla-M VLPs closely resembled the morphology and size of standard NiV-M VLPs, and both exhibited the standard pleomorphic shape representative of Paramyxoviridae, ranging in size from 50 nm to 800 nm. The images also resolved the presence of viral "spikes" protruding from the particles; these represent the viral envelope glycoproteins of NiV on the surface of the particle, confirming their incorporation into the VLPs. Tellingly, pseudotyped VSV+NiV-F/G particles resembled classical bullet-shaped Rhabdoviridae particles (Fig. 2b). This further underscores potential biological differences that may occur when using NiV-M based VLPs versus VSV pseudotypes.
Fig. 2c shows the specificity and sensitivity of our βla-M VLP entry assay via flow cytometry analyses. Entry of βla-M+NiV-F/G VLPs into Vero cells produced signals with a 25-fold dynamic range over βla-M VLPs lacking NiV viral envelope proteins (Fig. 2c). For simplicity, we will refer to successful entry of βla-M+NiV-F/G VLPs into susceptible cells as "infection" and to βla-M VLPs lacking NiV viral envelope proteins as "bald" VLPs. To verify receptor-specificity within our assay, we infected in the presence of soluble NiV receptor, ephrinB2-Fc, which successfully inhibited infection while a non-receptor homologue, ephrinB1-Fc, did not (Fig. 2c). In addition, anti-NiV-F and anti-NiV-G polyclonal antibodies[10, 37], but not the pre-immune sera, also inhibited infection (Fig. 2c) emphasizing that the βla-M+NiV-F/G VLPs emulate the known roles of F and G in mediating paramyxoviral entry. Green to blue color shifts in CCF2-loaded cells were also confirmed visually (Fig. 2d) before flow analyses. Collectively, these data establish that the βla-M VLPs physically and biochemically resemble NiV while the infection reflects the receptor and envelope specificity of live Nipah viruses.
βla-M+NiV-F/G VLPs infect biologically relevant cells in a receptor-dependent manner
Hendra virus (HeV) envelope proteins package efficiently onto βla-M(NiV) and produce infectious VLPs
βla-M VLPs enveloped with the NiV-GE505A mutant recapitulate differential receptor usage
A Y105W mutation within the active site of βla increases cleavage efficiency resulting in a more sensitive entry assay
Many viral entry studies on highly pathogenic agents have relied on cell-cell fusion and envelope pseudotyped reporter assays which have permitted detailed analyses of their entry characteristics without high-level biosafety containment. Yet, these surrogate assays may not fully emulate the biological properties unique to the virus being studied. Cell-cell fusion assays do not mimic virus-cell fusion kinetics and are not constrained by the geometry of virus-cell fusion, and envelope pseudotyped viral systems reflect the virion morphology of the backbone virus rather than the parental virus from which the envelopes are derived. Such differences may confound accurate dissection of the entry pathway under study. Pseudotyped reporter virus assays also require efficient replication and transcription of the reporter gene in the cell type used, and thus, post-entry factors may influence the efficiency of reporter gene expression. For BSL-4 containment viruses like NiV and HeV, the problems are compounded by the limited availability of resources to confirm the results of surrogate assays in live henipaviruses. Thus, we sought to develop a system that more faithfully replicates the native henipavirus entry process. This will allow for a more detailed and biologically relevant analysis of early entry events and will facilitate the development of high-throughput screens for inhibitors of bona fide henipavirus entry processes.
VLPs can be produced via expression of viral matrices alone or in combination with their respective envelope proteins [11–19]. Paramyxoviral matrix proteins, abundant within the virion, seemingly act as the 'bandleader' by coordinating several events within the viral life cycle: envelope protein localization, assembly and budding, nucleocapsid or genome recruitment, and particle disassembly or uncoating[11, 47]. Thus, these VLPs more faithfully mimic their live virus counterparts and permit a more biologically relevant analysis of entry and uncoating kinetics. Despite these many functionalities, none appear to be significantly disrupted by fusing large reporter proteins like GFP, Renilla luciferase, or βla to the N-terminus of NiV-M (Fig. 2 and unpublished observations). Thus, we sought to exploit this property by fusing the β-lactamase enzyme to the N-terminus of NiV-M in an effort to create a sensitive and specific viral entry assay.
Several viral entry assays have been developed that rely on cytosolic delivery, or intravirion detection, of a virion associated reporter fusion protein. For example, entry assays using vpr-βla for HIV and βla-matrix for Ebola have been described[48,49], yet the published assays would appear to be less sensitive than our current system[48, 50]. In the process of making our βla-M reporter, we discovered that both the NiV-M and the βla genes tended to use rare mammalian codons (Fig. 1a and see Additional file 1). Indeed, our βla-M fusion construct yielded significant protein expression only when both genes were fully codon-optimized (Fig. 1b-c and data not shown). This could explain why NiV-M is poorly expressed in the absence of vaccinia augmentation and why βla based real-time fusion assays are more sensitive and robust when using codon-optimized βla. Codon-optimization alone likely results in the larger dynamic range and greater sensitivity of our βla-M based assays.
Our βla-M VLPs adopt the pleomorphic morphology of paramyxoviruses and incorporate henipaviral envelopes in a manner indistinguishable from wild-type NiV-M VLPs. NiV and HeV envelope bearing βla-M VLPs recapitulate their biological phenotypes in terms of receptor usage and the requirements for F and G in the paramyxoviral entry process (Figs. 2, 3, 4 and 5). Importantly, βla-M VLPs can be used to study early entry events in primary cell targets of henipavirus infections, such as HMVECS, without potentially confounding factors like virus replication mediated cytotoxicity or other post-entry restriction factors. Significantly, the βla-M VLPs can also assay virus uncoating (i.e. virus-cell content mixing) via detection of viral matrix protein exposure to the cellular cytoplasm.
While it is clear that Rhabdoviridae can functionally accommodate many different heterologous envelopes [51–54], it is less clear whether paramyxoviral matrix proteins have the ability to functionally cross-complement other members of the family. We demonstrated here that βla-M(NiV) was able to complement and package the HeV envelope proteins, emphasizing the relatedness between these two viruses. Our results open the possibility that other paramyxoviral envelope proteins can functionally cross-complement onto βla-M(NiV), or their own respective βla-matrix fusion constructs, thereby providing a more efficient and high-throughput assay to study paramyxoviral entry. Arguably, short of reverse genetics to study matrix and envelope mutants in the context of parent paramyxoviruses, this βla-M VLP assay better reflects the native biology of paramyxoviral entry than other surrogate assays. To further improve the sensitivity of this assay for high-throughput applications, we exploited the vast literature on β-lactam structure-function studies and engineered a Y105W mutation into the active site of βla known to increase the cleavage efficiency of the enzyme [43–46] (Fig. 6).
In summary, we have developed a codon-optimized catalytically improved βla-M based VLP system that can be used for henipaviral entry studies. The flexibility of using either colorimetric or cell permeant fluorimetric substrates in the same βla-M VLP system allows for efficient, quantitative, and more high throughput analyses of henipavirus fusion and entry characteristics that more closely reflect those of authentic viral particles. Whether βla-M can be complemented with other paramyxoviral envelopes remains to be determined, but such studies will provide information into the specificity of matrix-envelope interactions. Lastly, our results imply that such a codon-optimized, catalytically improved βla-M based entry system may be adapted to other viruses that possess a matrix protein primarily responsible for virion morphology and budding characteristics.
Codon optimization and expression plasmids
The codon-optimized NiV-F or HeV-F and NiV-G or HeV-G gene products were tagged at their C-termini with an AU1 or hemagglutinin (HA) tag, respectively, as previously described[37, 39]. NiV-M WT was synthesized by Origene (Rockville, MD). GeneArt (Regensburg, Germany) performed mammalian codon-optimization of the NiV-M gene (M) product according to in-house proprietary software that addresses codon usage, elimination of cryptic splicing sites, as well as the stability of DNA/RNA secondary structures. NiV-M was subcloned into pcDNA3.1 (Invitrogen, Carlsbad, CA) between HindIII and XhoI restriction enzyme sites. The sequence of the codon-optimized NiV-M has been deposited into GenBank (Accession: EU480491). Origene (Rockville, MD) codon-optimized the βla gene, which was then subcloned into a pVAX1 (Invitrogen) expression vector between the KpnI and XhoI restriction enzyme sites. The sequence of the mammalian codon-optimized βla has been deposited into GenBank (Accession: EU744548). The βla gene was fused upstream of the NiV-M gene by overlap PCR and subsequently cloned into pcDNA3.1 via flanking KpnI and XhoI restriction enzyme sites with a NotI restriction enzyme site engineered in between the two genes. A single Y105W amino acid mutation within the βla active site was introduced using site-directed mutagenesis with QuikChange™ (Stratagene, La Jolla, CA). βlaY 105Wwas then cloned into pcDNA3.1 via flanking KpnI and NotI restriction enzyme sites. All gene products were confirmed by sequencing.
Production protocols to provide polyclonal antibodies (Rb. #2702, terminal bleed) via immunized rabbits (using a 20-mer antigenic peptide sequence corresponding to amino acids 29-49 of NiV-M) were generated by the Pinnacle Antibody Program (21st Century Biochemicals, Marlboro, MA). Monoclonal anti-HeV specific antibodies were produced by expressing HeV-F, HeV-G, and NiV-M in rabbits then isolating and screening specific anti-HeV lymphocytes from rabbit spleens as previously described for anti-NiV-F specific monoclonal antibodies.
293T cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) containing 10% fetal bovine serum (FBS) (Omega Scientific, Tarzana, CA). Vero cells were grown in minimal essential medium alpha (Invitrogen), containing 10% FBS. CHO stable cell lines expressing ephrinB2 or ephrinB3 were derived and maintained as previously described. HMVECs were grown in EGM-2 media supplemented with the MV Bullet Kit (Cambrex, Baltimore, MD). 293T and Vero cells were purchased from the ATCC. HMVEC cells were a kind gift from R. Shao.
Production of βla-M(NiV) VLPs
βla-M expression plasmids (25 μg) and either NiV-F and G or HeV-F and G (10 μg each) or pcDNA3 (20 μg) expression plasmids were transfected into 10 cm dishes of 293T cells using Lipofectamine 2000 (Invitrogen). At 24 h post-transfection, supernatants were collected and clarified before pelleting the VLPs at 110,000 g through a 20% sucrose (in PBS) cushion followed by resuspension in PBS (Invitrogen) containing 5% sucrose.
Immunoblotting of VLP proteins
βla-M VLP-containing supernatants were lysed and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently detected by immunoblotting using rabbit-anti-NiV-matrix (to detect all NiV-M proteins), goat-anti-HA-HRP (to detect all G proteins) (Novus Biologicals, Littleton, CO), or mouse-anti-AU1 (to detect all F proteins) (Covance, Princeton, NJ) antibodies. Primary and secondary antibodies were used at 1:1,000 and 1:80,000 dilutions, respectively, or 1:10,000 for anti-HA-HRP followed by FEMTO (Pierce, Rockford, IL) detection. Due to the similar molecular weights of βla-M (~70 kDa) and NiV-G (~67 kDa), membranes were probed for NiV-M, NiV-F or HeV-F, and NiV-G or HeV-G individually.
200-mesh Formvar carbon-coated copper grids (Electron Microscopy Sciences, Hatfield, PA) were floated on drops of the NiV VLP suspensions at room temperature, then blotted and stained with 1% aqueous uranyl acetate (UA) for NiV VLPs and 2% aqueous solution of phosphotungstic acid (PTA) for VSV particles. Electron microscopy studies were performed on a Philips 201 electron microscope at 70 kV.
Quantification of βla-M VLP entry via FACS Aria
Cells were plated into 24-well plates at a confluency of 75% and spinoculated (2,000 g) with βla-M VLPs for 2 h at 37°C. Although not required for efficient VLP entry, spinoculation has been shown to significantly improve the entry efficiency of several viruses (e.g. HIV, HHV-6, CMV) into target cells[55, 56] and, indeed, improved the signal to noise ratio within our assay (data not shown). Target cells were then stained with CCF2-AM substrate according to the manufacturer recommendations (Panvera, Madison, WI). The enzymatic reaction was allowed to take place at 25°C for 18 h. The cells were then washed, resuspended in FACS-buffer (2% FBS in PBS) and fixed with 2% paraformaldehyde. Cells were then acquired using FACS-Diva software on a FACS Aria machine (BD Biosciences, San Diego, CA) with excitation at 407 nm and emission at 520 nm and 447 nm. Samples were analyzed using FACS Convert and FCS Express v3 (De Novo Software, Los Angeles, CA). Soluble ephrinB1-Fc and ephrinB2-Fc fusion proteins were purchased from R&D Systems (Minneapolis, MN). Data were analyzed by GraphPad™ Prism Software (San Diego, CA) and represented as percentage infection (% blue positive cells).
We thank members of the Lee lab, especially Jennifer Fulcher for technical assistance and Frederic Vigant for quintessential review of the manuscript. This work was supported by NIH grants AI069317, AI060694, AI070495, and AI059051 to B.L. M.C.W. was supported by NIH grant AI07323 and the UCLA Warsaw Fellowship. We greatly appreciate all the time and wonderful assistance given from Stephanie Matyas at the Center For Aids Research flow cytometry core supported by NIH grants CA16042 and AI28697.
- Selvey LA, Wells RM, McCormack JG, Ansford AJ, Murray K, Rogers RJ, Lavercombe PS, Selleck P, Sheridan JW: Infection of humans and horses by a newly described morbillivirus. Med J Aust 1995, 162: 642-645.PubMedGoogle Scholar
- Field H, Young P, Yob JM, Mills J, Hall L, Mackenzie J: The natural history of Hendra and Nipah viruses. Microbes Infect 2001, 3: 307-314. 10.1016/S1286-4579(01)01384-3View ArticlePubMedGoogle Scholar
- O'Sullivan JD, Allworth AM, Paterson DL, Snow TM, Boots R, Gleeson LJ, Gould AR, Hyatt AD, Bradfield J: Fatal encephalitis due to novel paramyxovirus transmitted from horses. Lancet 1997, 349: 93-95. 10.1016/S0140-6736(96)06162-4View ArticlePubMedGoogle Scholar
- Wolf MC, Negrete OA, Lee B: Pathobiology of henipavirus entry: insights into therapeutic strategies. Future Virology 2007,2(3):267-282. 10.2217/174607188.8.131.527View ArticleGoogle Scholar
- Bossart KN, Wang LF, Flora MN, Chua KB, Lam SK, Eaton BT, Broder CC: Membrane fusion tropism and heterotypic functional activities of the Nipah virus and Hendra virus envelope glycoproteins. J Virol 2002, 76: 11186-11198. 10.1128/JVI.76.22.11186-11198.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Pager CT, Wurth MA, Dutch RE: Subcellular localization and calcium and pH requirements for proteolytic processing of the Hendra virus fusion protein. J Virol 2004, 78: 9154-9163. 10.1128/JVI.78.17.9154-9163.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Moll M, Kaufmann A, Maisner A: Influence of N-glycans on processing and biological activity of the nipah virus fusion protein. J Virol 2004, 78: 7274-7278. 10.1128/JVI.78.13.7274-7278.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Negrete OA, Levroney EL, Aguilar HC, Bertolotti-Ciarlet A, Nazarian R, Tajyar S, Lee B: EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus. Nature 2005, 436: 401-405.PubMedGoogle Scholar
- Negrete OA, Wolf MC, Aguilar HC, Enterlein S, Wang W, Muhlberger E, Su SV, Bertolotti-Ciarlet A, Flick R, Lee B: Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus. PLoS Pathog 2006, 2: e7. 10.1371/journal.ppat.0020007PubMed CentralView ArticlePubMedGoogle Scholar
- Aguilar HC, Matreyek KA, Filone CM, Hashimi ST, Levroney EL, Negrete OA, Bertolotti-Ciarlet A, Choi DY, McHardy I, Fulcher JA, et al.: N-glycans on Nipah virus fusion protein protect against neutralization but reduce membrane fusion and viral entry. J Virol 2006, 80: 4878-4889. 10.1128/JVI.80.10.4878-4889.2006PubMed CentralView ArticlePubMedGoogle Scholar
- Takimoto T, Portner A: Molecular mechanism of paramyxovirus budding. Virus Res 2004, 106: 133-145. 10.1016/j.virusres.2004.08.010View ArticlePubMedGoogle Scholar
- Schmitt AP, Lamb RA: Escaping from the cell: assembly and budding of negative-strand RNA viruses. Curr Top Microbiol Immunol 2004, 283: 145-196.PubMedGoogle Scholar
- Takimoto T, Murti KG, Bousse T, Scroggs RA, Portner A: Role of matrix and fusion proteins in budding of Sendai virus. J Virol 2001, 75: 11384-11391. 10.1128/JVI.75.23.11384-11391.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Sugahara F, Uchiyama T, Watanabe H, Shimazu Y, Kuwayama M, Fujii Y, Kiyotani K, Adachi A, Kohno N, Yoshida T, Sakaguchi T: Paramyxovirus Sendai virus-like particle formation by expression of multiple viral proteins and acceleration of its release by C protein. Virology 2004, 325: 1-10. 10.1016/j.virol.2004.04.019View ArticlePubMedGoogle Scholar
- Coronel EC, Murti KG, Takimoto T, Portner A: Human parainfluenza virus type 1 matrix and nucleoprotein genes transiently expressed in mammalian cells induce the release of virus-like particles containing nucleocapsid-like structures. J Virol 1999, 73: 7035-7038.PubMed CentralPubMedGoogle Scholar
- Timmins J, Scianimanico S, Schoehn G, Weissenhorn W: Vesicular release of ebola virus matrix protein VP40. Virology 2001, 283: 1-6. 10.1006/viro.2001.0860View ArticlePubMedGoogle Scholar
- Noda T, Sagara H, Suzuki E, Takada A, Kida H, Kawaoka Y: Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol 2002, 76: 4855-4865. 10.1128/JVI.76.10.4855-4865.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Jasenosky LD, Neumann G, Lukashevich I, Kawaoka Y: Ebola virus VP40-induced particle formation and association with the lipid bilayer. J Virol 2001, 75: 5205-5214. 10.1128/JVI.75.11.5205-5214.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Mebatsion T, Konig M, Conzelmann KK: Budding of rabies virus particles in the absence of the spike glycoprotein. Cell 1996, 84: 941-951. 10.1016/S0092-8674(00)81072-7View ArticlePubMedGoogle Scholar
- Lamb RA, Kolakofsky D: Paramyxoviridae: the viruses and their replication. In Fields Virology. Fourth edition. Edited by: Knipe D, MaH PM. Philadelphia: Lippincott Williams and Wilkins; 2001:689-724.Google Scholar
- Patch JR, Crameri G, Wang LF, Eaton BT, Broder CC: Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix protein. Virol J 2007, 4: 1. 10.1186/1743-422X-4-1PubMed CentralView ArticlePubMedGoogle Scholar
- Ciancanelli MJ, Basler CF: Mutation of YMYL in the Nipah virus Matrix Protein Abrogates Budding and Alters Subcellular Localization. J Virol 2006, 80: 12070-12078. 10.1128/JVI.01743-06PubMed CentralView ArticlePubMedGoogle Scholar
- Coronel EC, Takimoto T, Murti KG, Varich N, Portner A: Nucleocapsid incorporation into parainfluenza virus is regulated by specific interaction with matrix protein. J Virol 2001, 75: 1117-1123. 10.1128/JVI.75.3.1117-1123.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Bieniasz PD: Late budding domains and host proteins in enveloped virus release. Virology 2006, 344: 55-63. 10.1016/j.virol.2005.09.044View ArticlePubMedGoogle Scholar
- Schmitt AP, Leser GP, Morita E, Sundquist WI, Lamb RA: Evidence for a new viral late-domain core sequence, FPIV, necessary for budding of a paramyxovirus. J Virol 2005, 79: 2988-2997. 10.1128/JVI.79.5.2988-2997.2005PubMed CentralView ArticlePubMedGoogle Scholar
- Gao W, Xing B, Tsien RY, Rao J: Novel fluorogenic substrates for imaging beta-lactamase gene expression. J Am Chem Soc 2003, 125: 11146-11147. 10.1021/ja036126oView ArticlePubMedGoogle Scholar
- Raz E, Zlokarnik G, Tsien RY, Driever W: beta-lactamase as a marker for gene expression in live zebrafish embryos. Dev Biol 1998, 203: 290-294. 10.1006/dbio.1998.8999View ArticlePubMedGoogle Scholar
- Zlokarnik G, Negulescu PA, Knapp TE, Mere L, Burres N, Feng L, Whitney M, Roemer K, Tsien RY: Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter. Science 1998, 279: 84-88. 10.1126/science.279.5347.84View ArticlePubMedGoogle Scholar
- Irie T, Licata JM, Harty RN: Functional characterization of Ebola virus L-domains using VSV recombinants. Virology 2005, 336: 291-298. 10.1016/j.virol.2005.03.027PubMed CentralView ArticlePubMedGoogle Scholar
- Irie T, Licata JM, McGettigan JP, Schnell MJ, Harty RN: Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A. J Virol 2004, 78: 2657-2665. 10.1128/JVI.78.6.2657-2665.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Martin-Serrano J, Bieniasz PD: A bipartite late-budding domain in human immunodeficiency virus type 1. J Virol 2003, 77: 12373-12377. 10.1128/JVI.77.22.12373-12377.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Martin-Serrano J, Perez-Caballero D, Bieniasz PD: Context-dependent effects of L domains and ubiquitination on viral budding. J Virol 2004, 78: 5554-5563. 10.1128/JVI.78.11.5554-5563.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Harty RN, Brown ME, Wang G, Huibregtse J, Hayes FP: A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: implications for filovirus budding. Proc Natl Acad Sci USA 2000, 97: 13871-13876. 10.1073/pnas.250277297PubMed CentralView ArticlePubMedGoogle Scholar
- Harty RN, Paragas J, Sudol M, Palese P: A proline-rich motif within the matrix protein of vesicular stomatitis virus and rabies virus interacts with WW domains of cellular proteins: implications for viral budding. J Virol 1999, 73: 2921-2929.PubMed CentralPubMedGoogle Scholar
- Chong LD, Rose JK: Interactions of normal and mutant vesicular stomatitis virus matrix proteins with the plasma membrane and nucleocapsids. J Virol 1994, 68: 441-447.PubMed CentralPubMedGoogle Scholar
- Eaton BT, Mackenzie JS, Wang LF: Henipaviruses. In Fields Virology. Fifth edition. Edited by: Knipe D, MaH PM. Philadelphia: Lippincott Williams and Wilkins; 2007:1587-1598.Google Scholar
- Aguilar HC, Matreyek KA, Choi DY, Filone CM, Young S, Lee B: Polybasic KKR Motif in the Cytoplasmic Tail of Nipah Virus Fusion Protein Modulates Membrane Fusion by Inside-Out Signaling. J Virology 2007, 81: 4520-4532. 10.1128/JVI.02205-06PubMed CentralView ArticlePubMedGoogle Scholar
- Wong KT, Shieh WJ, Kumar S, Norain K, Abdullah W, Guarner J, Goldsmith CS, Chua KB, Lam SK, Tan CT, et al.: Nipah virus infection: pathology and pathogenesis of an emerging paramyxoviral zoonosis. Am J Pathol 2002, 161: 2153-2167.PubMed CentralView ArticlePubMedGoogle Scholar
- Negrete OA, Chu D, Aguilar HC, Lee B: Single amino acid changes in the Nipah and Hendra virus attachment glycoprotein distinguishes ephrinB2 from ephrinB3 usage. J Virol 2007, 81: 10804-10814. 10.1128/JVI.00999-07PubMed CentralView ArticlePubMedGoogle Scholar
- Bossart KN, Tachedjian M, McEachern JA, Crameri G, Zhu Z, Dimitrov DS, Broder CC, Wang LF: Functional studies of host-specific ephrin-B ligands as Henipavirus receptors. Virology 2007, 372: 357-371. 10.1016/j.virol.2007.11.011View ArticlePubMedGoogle Scholar
- Su AI, Wiltshire T, Batalov S, Lapp H, Ching KA, Block D, Zhang J, Soden R, Hayakawa M, Kreiman G, et al.: A gene atlas of the mouse and human protein-encoding transcriptomes. Proc Natl Acad Sci USA 2004, 101: 6062-6067. 10.1073/pnas.0400782101PubMed CentralView ArticlePubMedGoogle Scholar
- Goh KJ, Tan CT, Chew NK, Tan PS, Kamarulzaman A, Sarji SA, Wong KT, Abdullah BJ, Chua KB, Lam SK: Clinical features of Nipah virus encephalitis among pig farmers in Malaysia. N Engl J Med 2000, 342: 1229-1235. 10.1056/NEJM200004273421701View ArticlePubMedGoogle Scholar
- Matagne A, Lamotte-Brasseur J, Frere JM: Catalytic properties of class A beta-lactamases: efficiency and diversity. Biochem J 1998,330(Pt 2):581-598.PubMed CentralView ArticlePubMedGoogle Scholar
- Di Gleria K, Halliwell CM, Jacob C, Hill HA: Site-specific introduction of an electroactive label into a non-electroactive enzyme (beta-lactamase I). FEBS Lett 1997, 400: 155-157. 10.1016/S0014-5793(96)01373-7View ArticlePubMedGoogle Scholar
- Escobar WA, Miller J, Fink AL: Effects of site-specific mutagenesis of tyrosine 105 in a class A beta-lactamase. Biochem J 1994,303(Pt 2):555-558.PubMed CentralView ArticlePubMedGoogle Scholar
- Doucet N, De Wals PY, Pelletier JN: Site-saturation mutagenesis of Tyr-105 reveals its importance in substrate stabilization and discrimination in TEM-1 beta-lactamase. J Biol Chem 2004, 279: 46295-46303. 10.1074/jbc.M407606200View ArticlePubMedGoogle Scholar
- Peeples ME: Paramyxovirus M proteins: pulling it all together and taking it on the road. In The Paramyxoviruses. Edited by: Kingsbury DW. New York: Plenum; 1991:427-456.View ArticleGoogle Scholar
- Cavrois M, De Noronha C, Greene WC: A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes. Nat Biotechnol 2002, 20: 1151-1154. 10.1038/nbt745View ArticlePubMedGoogle Scholar
- Yonezawa A, Cavrois M, Greene WC: Studies of ebola virus glycoprotein-mediated entry and fusion by using pseudotyped human immunodeficiency virus type 1 virions: involvement of cytoskeletal proteins and enhancement by tumor necrosis factor alpha. J Virol 2005, 79: 918-926. 10.1128/JVI.79.2.918-926.2005PubMed CentralView ArticlePubMedGoogle Scholar
- Simmons G, Rennekamp AJ, Chai N, Vandenberghe LH, Riley JL, Bates P: Folate receptor alpha and caveolae are not required for Ebola virus glycoprotein-mediated viral infection. J Virol 2003, 77: 13433-13438. 10.1128/JVI.77.24.13433-13438.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Owens RJ, Rose JK: Cytoplasmic domain requirement for incorporation of a foreign envelope protein into vesicular stomatitis virus. J Virol 1993, 67: 360-365.PubMed CentralPubMedGoogle Scholar
- Kahn JS, Schnell MJ, Buonocore L, Rose JK: Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins: RSV fusion protein can mediate infection and cell fusion. Virology 1999, 254: 81-91. 10.1006/viro.1998.9535View ArticlePubMedGoogle Scholar
- Takada A, Robison C, Goto H, Sanchez A, Murti KG, Whitt MA, Kawaoka Y: A system for functional analysis of Ebola virus glycoprotein. Proc Natl Acad Sci USA 1997, 94: 14764-14769. 10.1073/pnas.94.26.14764PubMed CentralView ArticlePubMedGoogle Scholar
- Jones SM, Feldmann H, Stroher U, Geisbert JB, Fernando L, Grolla A, Klenk HD, Sullivan NJ, Volchkov VE, Fritz EA, et al.: Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses. Nat Med 2005, 11: 786-790. 10.1038/nm1258View ArticlePubMedGoogle Scholar
- Pietroboni GR, Harnett GB, Bucens MR: Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro. J Virol Methods 1989, 24: 85-90. 10.1016/0166-0934(89)90010-4View ArticlePubMedGoogle Scholar
- O'Doherty U, Swiggard WJ, Malim MH: Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. J Virol 2000, 74: 10074-10080. 10.1128/JVI.74.21.10074-10080.2000PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.