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Figure 4 | Virology Journal

Figure 4

From: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Figure 4

Hendra virus (HeV) envelope proteins package efficiently onto βla-M(NiV) and produce infectious VLPs. a) VLPs collected from βla-M(NiV)+ HeV-F/G or βla-M(NiV)-only transfected 293T supernatant were purified as described in the materials and methods. VLPs were lysed and blotted for proteins using anti-HA (HeV-G), anti-AU1 (HeV-F), or anti-NiV-M antibodies. b) HMVECs were infected by βla-M(NiV)+ HeV-F/G VLPs in the presence of anti-HeV-F specific (mAb 36) or anti-NiV-F specific (mAb 66)[37] monoclonal antibodies with non-specific monoclonal antibodies as a negative control to a final concentration of 20 μg/ml. Infected cells (% blue positive) were quantified using flow cytometry with untreated (NoTx) entry normalized as 100%. Data shown as an average of singlets from three individual experiments ± SD.

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Virology Journal

ISSN: 1743-422X