Skip to main content
Figure 3 | Virology Journal

Figure 3

From: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Figure 3

βla-M+NiV-F/G VLPs infect a biologically relevant cell line in a receptor-dependent manner. a) HMVECs were infected with βla-M+NiV-F/G or βla-M-only VLPs and quantified via flow cytometry. Soluble ephrinB2-Fc or ephrinB1-Fc was added at a final concentration of 75 nM. Infected cells (% blue positive) were quantified using flow cytometry with untreated entry (NoTx) normalized as 100%. Data shown as an average of triplicates from three individual experiments ± SEM. b) Representative flow cytometry plots of the data from (3a). c) βla-M+NiV-F/G VLPs from (a) were diluted in increments and used to infect HMVECs as previously described. Infected cells (% blue positive) were quantified using flow cytometry. Data shown as singlets from a single representative experiment.

Back to article page

Virology Journal

ISSN: 1743-422X