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Figure 6 | Virology Journal

Figure 6

From: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Figure 6

A single amino acid (Y105W) mutation within the active site of βla increases cleavage efficiency resulting in a more sensitive entry assay. a) Vero cells were infected with βla-M, βlaY105W-M, βla-M+NiV-F/G and βlaY105W-M+NiV-F/G VLPs. Infected cells (% blue positive) were quantified using flow cytometry with βla-M+NiV-F/G infection normalized as 100%. Data shown as an average of triplicates from one representative experiment ± SD. b) VLPs were lysed and blotted for protein incorporation using anti-HA (NiV-G), anti-AU1 (NiV-F), and anti-NiV-M antibodies.

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Virology Journal

ISSN: 1743-422X