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Figure 5 | Virology Journal

Figure 5

From: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Figure 5

βla-M VLPs enveloped with the NiV-G E505A mutant recapitulate differential receptor usage. a) Enveloped βla-M VLPs incorporating an E505A mutation in NiV-G were used to infect CHO-B2 or CHO-B3 cells stably expressing only ephrin-B2 or ephrin-B3, respectively. Infected cells (% blue positive) were quantified using flow cytometry with ephrin-B2 mediated entry normalized as 100%. Data shown as an average of triplicates from three individual experiments ± SEM. b) VLPs from (5a) were lysed and blotted for protein incorporation using anti-HA (NiV-G/NiV-GE505A), anti-AU1 (NiV-F), or anti-NiV-M antibodies.

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Virology Journal

ISSN: 1743-422X