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VP39 of Spodoptera litura multicapsid nucleopolyhedrovirus cannot efficiently rescue the nucleocapsid assembly of vp39-null Autographa californica multiple nucleopolyhedrovirus
Virology Journal volume 18, Article number: 81 (2021)
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) vp39 is conserved in all sequenced baculovirus genomes. In previous studies, VP39 has been identified as the major capsid structure protein of baculoviruses and found to be essential for nucleocapsid assembly. The nucleocapsid composition and structure of Group I and II NPVs of the Alphabaculovirus genus are very similar. It is not clear whether the major capsid structure protein VP39 of Group I NPVs is functionally identical to or substitutable with the Group II NPV VP39. In this study, the function of Group II Spodoptera litura MNPV (SpltMNPV) VP39 in Group I AcMNPV was characterized.
Sequence alignment of AcMNPV VP39 and SpltMNPV VP39 was performed using Clustal X and edited with GeneDoc. To determine whether VP39 of Group I NPVs can be functionally substituted by Group II NPV VP39, a vp39-null AcMNPV (vAcvp39KO) and a vp39-pseudotyped AcMNPV (vAcSpltvp39:FLAG), in which the Group I AcMNPV vp39 coding sequence was replaced with that of SpltMNPV from Group II NPVs, were constructed via homologous recombination in Escherichia coli. Using an anti-FLAG monoclonal antibody, immunoblot analysis was performed to examine SpltMNPV VP39 expression. Fluorescence and light microscopy were used to monitor viral replication and infection. Viral growth curve analysis was performed using a fifty percent tissue culture infective dose (TCID50) endpoint dilution assay. Viral morphogenesis was detected using an electron microscope.
Sequence alignment indicated that the N-termini of AcMNPV VP39 and SpltMNPV VP39 are relatively conserved, whereas the C-terminus of SpltMNPV VP39 lacks the domain of amino acid residues 306–334 homologous to AcMNPV VP39. Immunoblot analysis showed that SpltMNPV VP39 was expressed in vAcSpltvp39:FLAG. Fluorescence and light microscopy showed that vAcSpltvp39:FLAG did not spread by infection. Viral growth curve analysis confirmed a defect in infectious budded virion production. Electron microscopy revealed that although masses of abnormally elongated empty capsid structures existed inside the nuclei of Sf9 cells transfected with vAcSpltvp39:FLAG, no nucleocapsids were observed.
Altogether, our results demonstrated that VP39 from SpltMNPV cannot efficiently substitute AcMNPV VP39 during nucleocapsid assembly in AcMNPV.
Baculoviridae is a family of enveloped, rod-shaped, large, circular, double-stranded DNA viruses . These viruses are composed of four genera: Alphabaculovirus, Betabaculovirus, Gammabaculovirus, and Deltabaculovirus . The members of the Alphabaculovirus genus can be further divided into Group I and II nucleopolyhedroviruses (NPVs) . Taxonomically, Autographa californica multiple NPV (AcMNPV) is the prototype of the Baculoviridae family. Two different forms of virions, namely, budded virions (BVs) and occlusion-derived virions (ODVs), are produced during the biphasic replication cycle of baculoviruses. BVs are required for spreading infections among susceptible insect cells and tissues, resulting in systemic infection. ODVs initiate primary infection in a susceptible host and are responsible for transmitting infections among insect hosts . The main difference between BVs and ODVs is the composition and origin of their envelopes, whereas their nucleocapsid structures appear to be similar and are composed of a nucleoprotein core and a cylindrical capsid sheath. Viral DNA is synthesized in the virogenic stroma (VS) and packaged into capsids to form nucleocapsids, and capsid assembly seems to be independent of viral DNA packaging .
VP39, the major capsid structure protein, is arranged in stacked rings around the nucleoprotein core with monomers [6, 7]. The AcMNPV vp39 (orf89) gene is located between AcMNPV nucleotides (nt) 75,534 and 76,577 . Homologs of AcMNPV vp39 are present in all sequenced baculovirus genomes. Deletion of vp39 from Bombyx mori NPV (BmNPV) resulted in no obvious BV production . Recently, in a study reported by Bai et al., deletion of AcMNPV vp39 led to a defect in nucleocapsid assembly . The conserved glycine residue 276 plays an important role in the BmNPV VP39 function, including the structural assembly of capsids and viral DNA packaging .
The nucleocapsid composition and structure are very similar between Group I and II NPVs of the Alphabaculovirus genus, whereas their gene content and BV fusion protein are significantly different [1, 12]. It is not clear whether the major capsid structure protein VP39 of Group I NPVs is functionally identical to or substitutable with the Group II NPV VP39. The amino acid sequence identity of VP39 ranges from 35.28% to 51.32% between Group I and Group II NPVs and ranges from 37.22% to 45.71% between AcMNPV and Group II NPVs. The overall amino acid sequence identity between AcMNPV VP39 and SpltMNPV VP39 is 37.22% . Sequences of AcMNPV VP39 and SpltMNPV VP39 were aligned and edited using Clustal X  and GeneDoc , respectively. As shown in Fig. 1, AcMNPV VP39 and SpltMNPV VP39 are relatively conserved in the N-terminus, whereas SpltMNPV VP39 lacks a domain of amino acid residues 306–334 homologous to AcMNPV VP39 in the C-terminus. To determine whether the major capsid structure protein VP39 in Group I AcMNPV can be functionally substituted with Group II SpltMNPV VP39, we produced a recombinant AcMNPV (vAcSpltvp39:FLAG), in which AcMNPV vp39 was replaced with SpltMNPV vp39.
To determine whether SpltMNPV VP39 could function in AcMNPV, the bMON14272 bacmid was employed to generate a vp39-knockout AcMNPV bacmid (bAcvp39KO) via the λ Red recombination system. According to AcMNPV transcriptomics , the late transcription start site of cg30 and early transcription start site of lef4 were located 759 nt and 37 nt downstream of the initiation codon ATG of vp39, respectively. In the bAcvp39KO bacmid, a 344-bp region of vp39 (AcMNPV nt 75,934–76,277, between 300 and 645 nt downstream of the vp39 ATG) was replaced with a 1038-bp chloramphenicol resistance (Cm) gene cassette, with 300 nt of the 5′-end and 400 nt of the 3′-end of the vp39 ORF retained to avoid any potential effects on cg30 and lef4 (Fig. 2a). The absence of vp39 and replacement with the Cm gene in the AcMNPV bacmid were confirmed by PCR analysis. As shown in Fig. 2b, primers Acvp39PF2 (5′-GAGCTCCAAATTTGATTTCAATTTTATCGTGTTGGT-3′)/Acvp39PR2 (5′-GGATCCTTACTTATCGTCGTCATCCTTGTAATCGACGGCTATTCCTCCACCTGCTTC-3′) produced a 1468-bp PCR product in AcMNPV bacmid bMON14272 and a 2162-bp fragment in bAcvp39KO. Similarly, primers CmPF (5′-CCCTTTCGTCTTCGAATAAATACCT-3′)/CmPR (5′-TAAACCAGCAATAGACATAAGCGGC-3′), Acvp39PF2/CmPR, and CmPF/Acvp39PR2 produced no PCR product in AcMNPV bacmid bMON14272, but 1038-, 1738-, and 1438-bp fragments were amplified in bAcvp39KO, respectively. These results demonstrated that the Cm gene successfully replaced the target deletion region of vp39. The SpltMNPV vp39 gene (SpltMNPV nt 77,450–78,358) under the control of the AcMNPV vp39 promoter (AcMNPV nt 76,578–76,977) with a FLAG tag prior to the SpltMNPV vp39 stop codon, together with the enhanced green fluorescence protein (egfp; referred to as gfp in the present study) gene and the AcMNPV polyhedrin (polh) gene, which were driven by the AcMNPV ie1 promoter and its own promoter, respectively, were inserted into the polh locus of bAcvp39KO to construct the vp39-pseudotyped AcMNPV, vAcSpltvp39:FLAG (Fig. 2d). A vp39-knockout virus, vAcvp39KO, was used as a negative control and was constructed by inserting the polh and gfp genes into the polh locus of bAcvp39KO (Fig. 2d). Similarly, a wild-type AcMNPV, vAcWT, was used as a positive control and was generated by inserting the two genes into bMON14272 (Fig. 2c). A rescue virus, vAcvp39:FLAG, was constructed to confirm that the phenotype observed in vAcvp39KO resulted from the deletion of vp39 by inserting the vp39 gene under the control of its native promoter with a FLAG tag prior to the vp39 stop codon along with the polh and gfp genes into the polh locus of bAcvp39KO (Fig. 2d). To demonstrate that SpltMNPV VP39 could be expressed in vAcSpltvp39:FLAG, vAcvp39KO or vAcSpltvp39:FLAG bacmid DNA was transfected into Sf9 cells using Cellfectin and harvested at 72 h post transfection (p.t.). Using an anti-actin or anti-FLAG monoclonal antibody, western blotting was performed to detect actin (endogenous control) or SpltMNPV VP39 expression, respectively. SpltMNPV VP39 was detected in Sf9 cells transfected with vAcSpltvp39:FLAG but not in Sf9 cells transfected with vAcvp39KO (Fig. 2e).
To investigate the effect of vp39 replacement on viral replication, vAcvp39KO, vAcSpltvp39:FLAG, vAcvp39:FLAG, or vAcWT bacmid DNA was transfected into Sf9 cells, and viral replication and infection were monitored by fluorescence microscopy and light microscopy. At 24 h p.t., no obvious differences in the amounts of GFP-positive cells were observed among all four groups, indicating comparable transfection efficiencies (Fig. 3a). As expected, almost all Sf9 cells transfected with vAcWT or vAcvp39:FLAG bacmid DNA showed GFP fluorescence at 72 h p.t., and the number of fluorescent cells exhibited no detectable increase from 24 to 72 h p.t. in the vAcvp39KO-transfected cells. Notably, in vAcSpltvp39:FLAG-transfected cells, the variation in the number of fluorescent cells from 24 to 72 h p.t. was the same as that of vAcvp39KO, showing the inability of vp39-pseudotyped AcMNPV to produce infectious BVs to infect adjacent cells (Fig. 3a). In the four groups, occlusion body (OB) formation was observed using a light microscope (Fig. 3a), indicating that the infection progression among the four viruses was similar. Using virus supernatants harvested from vAcvp39KO-, vAcSpltvp39:FLAG-, vAcvp39:FLAG-, or vAcWT-transfected cells, viral growth curve analysis was performed using a fifty percent tissue culture infective dose (TCID50) endpoint dilution assay to further evaluate the ability of vAcSpltvp39:FLAG to produce infectious BV. No significant difference in the ability to generate infectious BVs was noted between vAcvp39:FLAG and vAcWT, whereas the infectious BVs of vAcvp39KO or vAcSpltvp39:FLAG were undetectable at any time point, even up to 120 h p.t. (Fig. 3b). These results showed that SpltMNPV VP39 cannot rescue the infectious BV production of vp39-deleted AcMNPV.
Thin sections of vAcvp39KO-, vAcSpltvp39:FLAG-, vAcvp39:FLAG-, and vAcWT-transfected Sf9 cells at 72 h p.t. were observed using an electron microscope to further investigate whether the replacement of vp39 interferes with virus morphogenesis (Fig. 4). Sf9 cells transfected with vAcWT (data not shown) or vAcvp39:FLAG showed typical characteristics of baculovirus infection, including a well-defined VS containing numerous rod-shaped, electron-dense nucleocapsids formed in the nuclei (Fig. 4a), numerous virus-induced intranuclear microvesicles emerged in the ring zone (data not shown), a large number of ODVs containing nucleocapsids (Fig. 4d) and developing OBs embedding numerous ODVs existed in the ring zone (Fig. 4g). In cells transfected with vAcvp39KO, although morphologically indistinguishable VS was observed in the nuclei (Fig. 4b), no capsid structures emerged in the VS (Fig. 4b) and ring zone (Fig. 4e). A large number of abnormal electron-dense bodies, which typically exist in AcMNPV-infected cells with anomalous nucleocapsids [17, 18], appeared in the VS (Fig. 4b). Numerous virus-induced intranuclear microvesicles existed in the ring zone (Fig. 4e), but no ODVs were observed in the ring zone (Fig. 4e) or the OBs (Fig. 4h). Similar to vAcvp39KO, in all vAcSpltvp39:FLAG-transfected Sf9 cells, a typical VS without nucleocapsids but with a large number of electron-dense bodies was observed in the nuclei (Fig. 4c), numerous virus-induced intranuclear microvesicles emerged in the ring zone (Fig. 4f), and no ODVs existed in the ring zone (Fig. 4f) or the OBs (Fig. 4i). However, interestingly, in vAcSpltvp39:FLAG-transfected Sf9 cells, many abnormally long electron-lucent capsid-like structures lacking an electron-dense core indicative of nucleoprotein that contains viral DNA were observed in the nuclei (Fig. 4f). These results demonstrated that SpltMNPV VP39 has the ability to assemble tubular capsid-like structures but not nucleocapsids in AcMNPV.
Similar to vAcSpltvp39:FLAG, the phenotypes of a series of nucleocapsid-associated gene individual deletion AcMNPV, such as pk-1, ac53, vp1054, vlf-1, vp91, 38 k, p6.9, BV/ODV-C42, and ac102, included the cessation of nucleocapsid assembly and the appearance of a large number of long empty capsid-like structures in the nucleus [17,18,19,20,21,22,23,24,25]. This finding emphasizes the need for further research to completely elucidate the mechanism of nucleocapsid assembly. Among the proteins encoded by these genes, P6.9 can bind baculoviral DNA and is responsible for baculoviral DNA condensation for packaging into capsids to form nucleocapsids [26, 27], and 38 K is critical for P6.9 to perform this function by dephosphorylating it .
Nucleocapsid assembly is a very complex process that demands numerous proteins to be involved in coordinatively. Previous studies showed that VP39 can interact with itself and other nucleocapsid-associated proteins and cellular proteins, including 38 K, ODV-EC27, BV/ODV-C42, P78/83, and actin [29,30,31,32], implying that these proteins may be jointly involved in crucial steps in nucleocapsid assembly.
Once proteins interact with each other, allosteric transition may confer a structure that differs from that noted for individual proteins . Here, we report that replacing AcMNPV vp39 with SpltMNPV vp39 resulted in interruption of nucleocapsid assembly, although a number of long capsid structures lacking viral DNA appeared in the nucleus. Considering that SpltMNPV VP39 lacks the homologous sequence of a C-terminal domain in AcMNPV VP39 (Fig. 1), which may lead to the structural difference between SpltMNPV VP39 and AcMNPV VP39, it is possible that, in the case of vAcSpltvp39:FLAG, SpltMNPV VP39 cannot appropriately interact with 38 K, ODV-EC27, BV/ODV-C42, P78/83, actin or other related proteins, or that the interactions are weak, which may affect the allosteric transition of these proteins. The incomplete transition might not allow proteins to be completely functional; for instance, the decreased activity of 38 K may not be enough to properly mediate the dephosphorylation of P6.9, thus resulting in no viral DNA packaging into capsids to form normal nucleocapsids in vAcSpltvp39:FLAG. Further studies are required to understand the details of this process.
In summary, our study showed that SpltMNPV vp39 substitution in AcMNPV interrupted the assembly of nucleocapsids, resulting in no BV or ODV being produced. To the authors’ knowledge, this is the first study reporting on the function of Group II SpltMNPV VP39 in Group I AcMNPV. Further studies are needed to disclose the molecular mechanisms of nucleocapsid assembly. These studies will likely provide information on the evolution of genes to be adapted for each virus-host interaction to facilitate virus replication and amplification in the host.
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Autographa californica multiple nucleopolyhedrovirus
Spodoptera litura MNPV
Bombyx mori NPV
- TCID50 :
Fifty percent tissue culture infective dose
- Cm :
- gfp :
Enhanced green fluorescence protein
- polh :
Rohrmann GF. Baculovirus Molecular Biology. 4th ed. National Library of Medicine: National Center. for Biotechnology Information, Bethesda, MD; 2019.
Virus Taxonomy: 2019 Release. EC 51, Berlin, Germany, July 2019. Email ratification March 2020 (MSL # 35), http://ictv.global/msl.htm.
de Zanotto PM, Kessing BD, Maruniak JE. Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invertebr Pathol. 1993;62:147–164.
Federici BA. Baculovirus pathogenesis. In: Miller LK, editor. The baculoviruses. New York: Plenum Press Inc; 1997. p. 33–56.
Wang Q, Bosch BJ, Vlak JM, van Oers MM, Rottier PJ, van Lent JWM. Budded baculovirus particle structure revisited. J Invertebr Pathol. 2016;134:15–22.
Pearson MN, Russell RL, Rohrmann GF, Beaudreau GS. P39, a major baculovirus structural protein: immunocytochemical characterization and genetic location. Virology. 1988;167:407–13.
Federici BA. Ultrastructure of baculoviruses. In: Granados RR and Federici BA (ed.). The Biology of Baculoviruses. CRC Press, Boca Raton, Florida, 1986. Vol. I, p61–88, 2 vols.
Ayres MD, Howard SC, Kuzio J, Lopez-Ferber M, Possee RD. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology. 1994;202:586–605.
Ono C, Kamagata T, Taka H, Sahara K, Asano S, Bando H. Phenotypic grouping of 141 BmNPVs lacking viral gene sequences. Virus Res. 2012;165:197–206.
Bai H, Hu Y, Hu X, Li J, Mu J, Zhou Y, et al. Major capsid protein of Autographa californica multiple nucleopolyhedrovirus contributes to the promoter activity of the very late viral genes. Virus Res. 2019;273:197758.
Katsuma S, Kokusho R. A conserved glycine residue is required for proper functioning of a baculovirus VP39 protein. J Virol. 2017;91.
Pearson MN, Rohrmann GF. Transfer, incorporation, and substitution of envelope fusion proteins among members of the Baculoviridae, Orthomyxoviridae, and Metaviridae (insect retrovirus) families. J Virol. 2002;76:5301–4.
Pang Y, Yu J, Wang L, Hu X, Bao W, Li G, et al. Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology. 2001;287:391–404.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947–2948.
Nicholas KB, Nicholas HB, Deerfield DW. GeneDoc: analysis and visualization of genetic variation. EMBNEW News. 1997;4:14.
Chen YR, Zhong S, Fei Z, Hashimoto Y, Xiang JZ, Zhang S, et al. The transcriptome of the baculovirus Autographa californica multiple nucleopolyhedrovirus in Trichoplusia ni cells. J Virol. 2013;87:6391–405.
Zhu S, Wang W, Wang Y, Yuan M, Yang K. The baculovirus core gene ac83 is required for nucleocapsid assembly and per os infectivity of Autographa californica nucleopolyhedrovirus. J Virol. 2013;87:10573–86.
Guan Z, Zhong L, Li C, Wu W, Yuan M, Yang K. The Autographa californica multiple nucleopolyhedrovirus ac54 gene is crucial for localization of the major capsid protein VP39 at the site of nucleocapsid assembly. J Virol. 2016;90:4115–26.
Liu C, Li Z, Wu W, Li L, Yuan M, Pan L, et al. Autographa californica multiple nucleopolyhedrovirus ac53 plays a role in nucleocapsid assembly. Virology. 2008;382:59–68.
Liang C, Li M, Dai X, Zhao S, Hou Y, Zhang Y, et al. Autographa californica multiple nucleopolyhedrovirus PK-1 is essential for nucleocapsid assembly. Virology. 2013;443:349–57.
Wu W, Lin T, Pan L, Yu M, Li Z, Pang Y, et al. Autographa californica multiple nucleopolyhedrovirus nucleocapsid assembly is interrupted upon deletion of the 38K gene. J Virol. 2006;80:11475–85.
Li K, Wang Y, Bai H, Wang Q, Song J, Zhou Y, et al. The putative pocket protein binding site of Autographa californica nucleopolyhedrovirus BV/ODV-C42 is required for virus-induced nuclear actin polymerization. J Virol. 2010;84:7857–68.
Hepp SE, Borgo GM, Ticau S, Ohkawa T, Welch MD. Baculovirus AC102 is a nucleocapsid protein that is crucial for nuclear actin polymerization and nucleocapsid morphogenesis. J Virol. 2018.
Wang M, Tuladhar E, Shen S, Wang H, van Oers MM, Vlak JM, et al. Specificity of baculovirus P6.9 basic DNA-binding proteins and critical role of the C terminus in virion formation. J Virol. 2010;84:8821–8.
Vanarsdall AL, Okano K, Rohrmann GF. Characterization of the role of very late expression factor 1 in baculovirus capsid structure and DNA processing. J Virol. 2006;80:1724–33.
Tweeten KA, Bulla LA, Consigli RA. Characterization of an extremely basic protein derived from granulosis virus nucleocapsids. J Virol. 1980;33:866–76.
Funk CJ, Consigli RA. Phosphate cycling on the basic protein of Plodia interpunctella granulosis virus. Virology. 1993;193:396–402.
Lai Q, Wu W, Li A, Wang W, Yuan M, Yang K. The 38K-Mediated specific dephosphorylation of the viral core protein P6.9 plays an important role in the nucleocapsid assembly of Autographa californica multiple nucleopolyhedrovirus. J Virol. 2018;92.
Lanier LM, Volkman LE. Actin binding and nucleation by Autographa california M nucleopolyhedrovirus. Virology. 1998;243:167–77.
Lu S, Ge G, Qi Y. Ha-VP39 binding to actin and the influence of F-actin on assembly of progeny virions. Arch Virol. 2004;149:2187–98.
Braunagel SC, Summers MD. Molecular biology of the baculovirus occlusion-derived virus envelope. Curr Drug Targets. 2007;8:1084–95.
Wu W, Liang H, Kan J, Liu C, Yuan M, Liang C, et al. Autographa californica multiple nucleopolyhedrovirus 38K is a novel nucleocapsid protein that interacts with VP1054, VP39, VP80, and itself. J Virol. 2008;82:12356–64.
Makalliwa GA, Wang X, Zhang H, Zhang N, Chen C, Li J, et al. HearNPV pseudotyped with PIF1, 2, and 3 from MabrNPV: infectivity and complex stability. Virol Sin. 2018;33:187–96.
The authors are grateful to Professors Kai Yang, Wenbi Wu, and Zhihong Huang of Sun Yat-sen University, China, for their valuable suggestions.
This work was supported by the Provincial Natural Science Foundation of Guangdong (2014A030313664), the National Natural Science Foundation of China (31501705), and the Provincial Regular University Key Research Platform and Project Foundation of Guangdong (2018KTSCX248).
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Li, S., Ou, B., Lv, Y. et al. VP39 of Spodoptera litura multicapsid nucleopolyhedrovirus cannot efficiently rescue the nucleocapsid assembly of vp39-null Autographa californica multiple nucleopolyhedrovirus. Virol J 18, 81 (2021). https://doi.org/10.1186/s12985-021-01553-9
- Nucleocapsid assembly
- Viral DNA packaging