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Fig. 4 | Virology Journal

Fig. 4

From: VP39 of Spodoptera litura multicapsid nucleopolyhedrovirus cannot efficiently rescue the nucleocapsid assembly of vp39-null Autographa californica multiple nucleopolyhedrovirus

Fig. 4

Electron microscopy of Sf9 cells transfected with vAcvp39:FLAG (a, d, and g), vAcvp39KO (b, e, and h), or vAcSpltvp39:FLAG (c, f, and i) at 72 h p.t. (a) Portion of virogenic stroma (VS) showing nucleocapsids with viral DNA. (b) and (c) Portion of VS without nucleocapsids, exhibiting abnormal electron-dense bodies (white arrows). (d) Portion of the ring zone with occlusion-derived virions (ODVs). (e) Portion of the ring zone without ODV, showing numerous microvesicles. (f) Portion of the ring zone without ODV, showing numerous microvesicles and masses of abnormally long electron-lucent capsid-like structures lacking viral DNA (white triangles). (g) Magnified view of occlusion body (OB) embedding with ODVs. (h) and (i) Magnified view of OB without ODVs. NC, nucleocapsid; RZ, ring zone; M, microvesicle; P, OB. Scale bar, 500 nm

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Virology Journal

ISSN: 1743-422X