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Fig. 3 | Virology Journal

Fig. 3

From: VP39 of Spodoptera litura multicapsid nucleopolyhedrovirus cannot efficiently rescue the nucleocapsid assembly of vp39-null Autographa californica multiple nucleopolyhedrovirus

Fig. 3

Analysis of viral replication. (a) Sf9 cells were transfected with vAcvp39KO, vAcSpltvp39:FLAG, vAcvp39:FLAG, or vAcWT bacmid DNA. Cells were observed using a fluorescence microscope at 24 and 72 h p.t. and a light microscope at 96 h p.t. (b) Sf9 cells were transfected with vAcvp39KO, vAcSpltvp39:FLAG, vAcvp39:FLAG, or vAcWT bacmid DNA. At designated time points, supernatants were harvested, and BV titers were determined using TCID50 endpoint dilution assays. Each value represents the average titer of three independent assays; error bars represent standard deviations

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Virology Journal

ISSN: 1743-422X