- Open Access
Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice
© Israyelyan et al; licensee BioMed Central Ltd. 2008
- Received: 10 April 2008
- Accepted: 02 June 2008
- Published: 02 June 2008
The NV1020 oncolytic herpes simplex virus type-1 has shown significant promise for the treatment of many different types of tumors in experimental animal models and human trials. Previously, we described the construction and use of the NV1020-like virus OncSyn to treat human breast tumors implanted in nude mice. The syncytial mutation gKsyn1 (Ala-to-Val at position 40) was introduced into the OncSyn viral genome cloned into a bacterial artificial chromosome using double-red mutagenesis in E. coli to produce the OncdSyn virus carrying syncytial mutations in both gB(syn3) and gK(syn1).
The OncdSyn virus caused extensive virus-induced cell fusion in cell culture. The oncolytic potential of the OncSyn and OncdSyn viruses was tested in the highly metastatic syngeneic mouse model system, which utilizes 4T1 murine mammary cancer cells implanted within the interscapular region of Balb/c mice. Mice were given three consecutive intratumor injections of OncSyn, OncdSyn, or phosphate buffered saline four days apart. Both OncSyn and OncdSyn virus injections resulted in significant reduction of tumor sizes (p < 0.05) compared to control tumors. Virus treated mice but not controls showed a marked reduction of metastatic foci in lungs and internal organs. Mouse weights were not significantly impacted by any treatment during the course of the entire study (p = 0.296).
These results show that the attenuated, but highly fusogenic OncSyn and OncdSyn viruses can effectively reduce primary and metastatic breast tumors in immuncompetent mice. The available bac-cloned OncSyn and OncdSyn viral genomes can be rapidly modified to express a number of different anti-tumor and immunomodulatory genes that can further enhance their anti-tumor potency.
- Bacterial Artificial Chromosome
- Vero Cell
- Oncolytic Virus
- Reduce Tumor Volume
- Oncolytic Virotherapy
Recent advances in molecular virology have enabled investigators to construct viruses that selectively destroy cancer cells (oncolytic virotherapy). Genetically engineered viruses belonging to different viral families have been evaluated for their potential as therapeutic agents in the treatment of malignant tumors [1–4]. Efficient replication, cell lysis and spread of HSV, and their natural broad host range make them attractive candidates as oncolytic viral agents [5–7]. Furthermore, the recent availability of cloned HSV genomes into bacterial artificial chromosome vectors greatly facilitates the rapid construction of new recombinant viruses carrying multiple transgenes of interest [8–10]. Tumor treatment with oncolytic HSV has been shown to induce anti-tumor immune responses [11–15]. Although the majority of people are seropositive for HSV-1, oncolytic virotherapy with HSV is not limited by pre-existing anti-HSV immunity [16, 17], and in at least one example, preexisting immunity to HSV-1 enhanced anti-tumor immune responses .
Recently, the NV1020 oncolytic herpes simplex virus type-1(HSV-1) was shown to have significant promise for the treatment of many different types of tumors in preclinical studies in experimental animals as well as in human clinical trials [17, 19–22]. The main advantage of this virus over other HSV oncolytic viruses is that it expresses one of the two original γ134.5 genes allowing the virus to replicate more efficiently, while safety is not compromised [23–26]. The γ134.5 gene is a major neurovirulence gene and an inhibitor of cellular apoptosis. Deletion of this gene drastically attenuates the virus and restricts viral growth to cancer cells because of their lack of intact apoptotic mechanisms [27, 28]. Preclinical studies in mice as well as phase I/II human trials have revealed that oncolytic HSV-1 viruses having both γ134.5 genes deleted did not spread efficiently within tumors . In contrast, deletion of one of the two γ134.5 genes drastically attenuated the virus, while allowing improved virus replication and spread in tumor cells [23–25]. The NV1020 was originally constructed for vaccine purposes and it contains HSV-2 viral sequences coding for glycoproteins gD, gG, gI and gE to facilitate production of anti-HSV-2 immune responses .
HSV can be transmitted from cell-to-cell by causing limited amounts of virus-induced cell fusion, thus avoiding the extracellular environment. Specific mutations within viral glycoproteins are known to greatly enhance virus-induced cell fusion. Specifically, syncytial mutations that cause extensive virus-induced cell fusion can arise in at least two of the glycoprotein genes: the UL27 gene, encoding glycoprotein B (gB) [30–32], and the UL53 gene, coding for glycoprotein K (gK) [33, 34]. Work in our laboratory has shown that gK functions as a heterodimer with the UL20 viral protein and the UL20/gK heterodimer is necessary for virus-induced cell fusion [35, 36].
The HSV-1 oncolytic virus Onc was constructed based on the NV1020 genomic arrangement with the exception that there are no genomic re-arrangements and no HSV-2 genes inserted within the viral genome. Recently, we reported that the OncSyn virus carrying a syncytial mutation in gB, enabling the virus to spread among cells by virus-induced cell fusion, replicated efficiently in breast cancer cells in vitro and drastically reduced tumor volumes in vivo . In this study we constructed and tested the OncdSyn virus, which in addition to the gBsyn3 mutation also carried the gKsyn1 mutation known to enable the virus to fuse even difficult to fuse cells . Intra-tumor injections of either virus effectively reduced tumor volumes as well as inhibited tumor metastases to internal organs.
Construction and characterization of the Oncolytic HSV-1 mutant virus OncdSyn
Phenotypic characteristics of the OncSyn and OncdSyn viruses on Vero and 4T1 cells
Kinetics of viral replication on Vero and 4T1 cells
Metastatic nodes in internal organs
No. of mice in group
No. of mice with metastases in internal organs
No. of mice with metastases in liver
No. of mice with metastases in spleen
No. of mice with metastases in kidney
Metastatic nodes in lungs
No. of mice in group
No. of metastatic nodes in lungs of experimental animalsa
The oncolytic HSV-1-based virus NV1020 has shown strong promise for treatment of different tumors in animal models and human clinical trials [17, 19–22]. To facilitate the construction of recombinant viruses carrying multiple transgenes of interest, we cloned the NV1020-like HSV-1 recombinant virus OncSyn into a bacterial artificial chromosome (bac) vector. The OncSyn virus specifies a syncytial mutation in gB (Arg-to-His change at aa 858) that increases its ability to spread in tumor cells via virus-induced cell fusion . In this study, we introduced the syncytial mutation syn1 (Ala-to-Val change at aa 40) within the gK gene to further enhance the fusogenicity of the new virus OncdSyn. The OncdSyn virus reduced primary tumor sizes and inhibited metastases to distal organs in the 4T1 syngeneic mouse model system.
The syn1 mutation within gK has been shown to produce extensive virus-induced cell fusion in all cells tested. In comparison, the gBsyn3 mutation produced virus-induced cell fusion in most cells, but it was unable to fuse certain hard to fuse cells, such as Hep-2 cells derived from human laryngeal carcinoma . Therefore, to further increase the ability of the OncSyn virus to fuse all types of cells, we generated the OncdSyn virus carrying both the gBsyn3 and gKsyn1 mutations. As expected, the OncdSyn virus caused extensive virus-induced cell fusion and fused Hep-2 cells, while the OncSyn virus did not (not shown). Furthermore, the OncdSyn virus caused more extensive fusion than OncSyn in both Vero and 4T1 cells. The OncdSyn virus appeared to produce intact syncytia that remained attached to the cell culture flasks, while the OncSyn virus-induced syncytia contained infected single cells, which detached easier than the OncdSyn-infected syncytia. This phenomenon has been previously observed for the gB and gK syncytial mutations and it is probably due, in part, to the extensive virus-induced cell fusion caused by the gK syncytial mutation, which appears to also fuse internal membranes such as nuclear membranes in addition to plasma membranes of cells (Kousoulas, unpublished). Viral titers of the OncdSyn virus were lower in Vero cells than titers of the OncSyn virus and substantially lower than titers of the OncSyn virus in 4T1 cells. Typically, HSV-1 syncytial mutants produce lower viral titers than their parental wild-type viruses, most likely because of their direct effect on cellular membranes. In this regard, the increased ability of the OncdSyn virus to cause extensive virus-induced cell fusion is probably responsible for the observed decrease in viral titers in comparison to the OncSyn virus.
Defects of viral replication and spread in mouse cancer cells have been described in the literature for oncolytic herpesviruses [11, 14]. HSV-1 does not replicate efficiently in mouse cell lines [41, 42] most likely because it cannot as efficiently utilize the mouse nectin-1 receptor, which is approximately 5% different in its amino acid sequence to the human nectin-1 receptor . Nectin-1 is also known to facilitate virus-induced cell fusion and virus-spread . Consequently, both OncSyn and OncdSyn viruses replicated much less efficiently in 4T1 cells than in Vero cells. In this regard, the limited replication and spread of these viruses in 4T1 cells would be expected to adversely affect their oncolytic ability in 4T1-derived tumors in vivo. Previously, we reported that the OncSyn virus effectively reduced primary human breast cancer tumors in nude mice . The disadvantage of the MDA-MB-435S human breast tumors is that these tumors would be rapidly eliminated if they were implanted in immunocompetent mice. Therefore, we chose the 4T1/Balb/c mouse model system for additional testing of both the previously constructed OncSyn virus as well as the newly constructed OncdSyn virus. Both OncSyn and OncdSyn viruses substantially reduced the growth of 4T1 tumors compared to the PBS controls, despite the fact that these viruses did not efficiently replicate in 4T1 cells in cell culture. Apparently, viral replication and infectious virus production in cell cultures did not correlate with the oncolytic efficacy of these viruses, because the OncdSyn virus reduced tumor volumes equally-well with the OncSyn virus, despite the fact that OncdSyn replicated approximately less than half a log than the OncSyn virus in 4T1 cells. Therefore, the relative increased ability of the OncdSyn virus to destroy tumors in vivo must be attributed to its enhanced fusogenicity.
Multiple murine tumor models have been used as preclinical settings for therapeutic purposes. The 4T1 mammary carcinoma model has several distinct advantages to be used as such model. It is regarded as a highly physiological, clinically-relevant mouse model that closely resembles stage IV human breast cancer in its properties . 4T1 cells are considered to be very weakly immunogenic (relative antigenic strength is less than 0.01 with 9.9 being the most immunogenic) [45, 46], and they spontaneously metastasize to distal parts of the body [40, 47]. Metastatic tumor foci in liver and spleen were substantially reduced in OncSyn and OncdSyn-treated mice in comparison to PBS-treated control mice. Reduction of metastatic foci in internal organs (lung, spleen, kidney and liver) was dependent on the size of the original 4T1 tumor, as well as the time of necropsy post implantation of tumor cells. Specifically, there was drastic reduction in tumor foci detected in lungs when the initial tumor size treated with the virus was approximately 35–40 mm3 and necropsies were performed at 33 days after tumor implantation. Furthermore, lungs appeared to have the same number of metastatic foci with PBS-treated controls when the initial treated tumors where 80–90 mm3 and necropsies were performed at day 42 after tumor implantation. This metastatic pattern revealed that lungs were the primary metastatic site of the subcutaneous implanted 4T1 cells. Regardless of the size of the initial tumor treated and the time of necropsies post tumor implantation, it was evident that OncSyn and OncdSyn viruses appeared to efficiently reduce the growth of the primary tumor as well as substantially inhibit or eliminate formation of metastatic foci.
It is highly likely that reduction of the primary tumor after oncolytic virotherapy with the OncSyn and OncdSyn viruses is responsible for the observed reduction in the formation of secondary tumor foci, since treatment of the smaller (35–40 mm3) tumors appeared to drastically reduce lung metastases. Alternatively, it is possible that anti-tumor immune responses were elicited by exposure of tumor antigens after destruction of 4T1 cells within the primary tumor by the OncSyn and OncdSyn viruses. In this regard, a fusogenic oncolytic HSV-1 Synco-2D was reported to elicit anti-tumor immune responses when studied in a similar animal model of mammary carcinoma utilizing 4T1 cells . A strong T-cell response was reported also by an HSV-2 derivative oncolytic virus FusOn-H2 effectively treating primary and metastatic mammary tumors in vivo .
Overall, our results showed that both OncSyn and OncdSyn viruses can efficiently reduce the primary and metastatic growth of 4T1 tumors established in immunocompetent mice. It is expected that these viruses would be even more efficacious against human breast cancer tumors by virtue of the fact that they can replicate substantially more efficiently (more than one log) in human than mouse cells. The availability of both OncSyn and OncdSyn viruses as bacterial artificial chromosomes will enable the generation of additional recombinant viruses that carry multiple anti-tumor and immunomodulatory transgenes, which could further enhance the anti-tumor efficacy of these viruses.
African green monkey kidney (Vero) cells and mouse mammary tumor cells (4T1)  were obtained from the American Type Culture Collection (Manassas, VA). Vero cells were maintained in Dulbecco's modified Eagle's medium (Gibco-BRL; Grand Island, N.Y.), supplemented with 10% fetal calf serum (FCS) and antibiotics. 4T1 cells were maintained in RPMI 1640 medium (Hyclone, Logan, UT) containing 10% FCS. The cultures were maintained at 37°C in a humidified atmosphere of 5% CO2/95% air.
Construction of the doubly fusogenic recombinant virus HSV-1 OncdSyn
The previously published OncSyn viral genome recovered as a bacterial artificial chromosome (bac) into E. coli (pOncSyn)  was used for the construction of pOncdSyn bac plasmid utilizing a new methodology – the double-red mutagenesis technique in E. coli  enabling the markerless introduction of the gKsyn1 mutation (Ala-toVal at aa 40). The OncdSyn virus was recovered after transfection of Vero cells with the pOncdSyn plasmid. The OncdSyn viral genome and the pOncdSyn bac were extensively characterized by diagnostic PCR and DNA sequencing to ensure the stability of the viral genomes, the presence of the parental Onc deletions and the presence of the gKsyn1 mutation within the gK gene, as described previously for the OncSyn virus .
Phenotypic characterization and replication kinetics of the OncSyn and OncdSyn viruses
Cells (both Vero and 4T1) were seeded into 6-well plates and infected the following day (when they reached approximately 95% confluency) with the OncSyn or OncdSyn viruses at a multiplicity of infection (MOI) ranging from 0.001–1 plaque forming units per cell (PFU/cell). Cells were cultured in a maintenance medium (containing 2% FCS) and were left for 2 days to allow for the plaques and the cell fusion to develop. Photographs of the infected cells were taken using a fluorescence microscope. For assessment of viral plaque morphologies, Vero and 4T1 cells were infected with HSV-1(F), OncSyn or OncdSyn viruses and visualized after immunohistochemistry at 48 hours post-infection (h.p.i.) using horseradish peroxidase-conjugated anti-HSV antibody (Dako, Carpinteri, CA) and Novared substrate development kit (VectorLabs, Burlingame, CA).
To determine the replication kinetics of the viruses, one-step growth kinetics were performed as described previously [48, 49]. Briefly, nearly confluent monolayers of either Vero or 4T1 cells were infected with each virus at an MOI of 2 at 4°C for 1 h. Thereafter, virus was allowed to penetrate for 2 h at 37°C. Any remaining extracellular virus was inactivated by low-pH treatment with phosphate buffered saline at pH 3.0. Cells and supernatants were harvested immediately thereafter (0 h) or after 12 or 24 h of incubation at 37°C. Virus titers were determined by endpoint titration of virus stocks on Vero cells.
Female Balb/c mice were obtained from Harlan (Indianapolis, IN) and housed in an animal room which was kept at 25°C with a 12 hour light-dark cycle. All experimental procedures involving animals were approved by the institutional animal care and use committee (IACUC) of the Louisiana State University. At 6–7 weeks of age the animals (19–20 g body weight) were implanted subcutaneously in the interscapular area with 1 × 105viable 4T1 cells suspended in 0.2 ml of PBS using a 27 gauge needle. Body weights were determined weekly, and tumor sizes were monitored beginning 7 days after tumor inoculation by direct measuring with a digital microcaliper. Tumor volumes were calculated using the following formula: volume = (length × width × height)/2. At an average tumor volume of approximately 80–90 mm3 (first experiment) or 35–40 mm3 (second experiment), animals were randomized into 3 groups (first experiment) or 2 groups (second experiment) using a randomization plan. The groups of mice received 3 intratumoral injections of the OncSyn, OncdSyn viral particles, or PBS every four days for the first experiment and injections of the OncdSyn or PBS every third day for the second experiment. Each tumor was injected with approximately 1 × 106 viruses per injection in 250 μl volume, while control mice received 250 μl of PBS. Injections were performed slowly at 3 different sites per tumor. On day 42 for the first experiment and day 33 for the second experiment after initial tumor cell implantation, mice were humanely euthanized in a CO2 chamber and subjected to gross as well as microscopic histological examination. Lung and other internal organ metastases were counted using a dissecting microscope after placing the resected organs in fixative for 24 hours. The primary tumor site, lungs, heart, liver, spleen, and kidneys from each animal were fixed in 10% neutral buffered formalin, trimmed, paraffin embedded, sectioned, stained with hematoxylin and eosin (H&E), and evaluated by light microscopy.
Statistical methods and analyses
The SAS® statistical package (Version 9.1.3) was used for the analyses of the in vivo studies. Distributions were examined for normality using the UNIVARIATE procedure with a Shapiro-Wilk test of normality. For the repeated measures part of the analyses of tumor volumes and tumor weights, the GLM procedure was used to conduct a repeated measures design analyzed as a split-plot arrangement of treatments with TREATMENT (OncSyn, OncdSyn, and PBS) and MOUSE within TREATMENT as main plot factors. Subplot factors included PERIOD (days of measurements) and TREATMENT by PERIOD interaction. When overall analyses determined significance (p = 0.05), Tukey's HSD test was used to examine pairwise differences for main effects, and pairwise comparisons of least square means with regard to interaction effects were examined with preplanned t-tests. The Wilcoxon Two-Sample test was used to analyze the difference of lung metastatic node counts between PBS and OncdSyn groups.
This work was supported by the grant "Novel Cancer Treatment Modalities" from the Louisiana Governor's Biotechnology Initiative (GBI), Louisiana Board of Regents and by the grant R01 AI43000 from NIH:NIAID to K.G.K. The project was also supported by the Louisiana Gene Therapy Program of the LSU Health Sciences Center, New Orleans. The authors gratefully acknowledge BioMMED's support by the LSU School of Veterinary Medicine and helpful discussions with Marlene Orandle and Karen Peterson (Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University).
- Chiocca EA: Oncolytic viruses. Nat Rev Cancer 2002, 2: 938-950. 10.1038/nrc948View ArticlePubMedGoogle Scholar
- Kirn D: Oncolytic virotherapy as a novel treatment platform for cancer. Ernst Schering Res Found Workshop 2003, 89-105.Google Scholar
- Nemunaitis J, Edelman J: Selectively replicating viral vectors. Cancer Gene Ther 2002, 9: 987-1000. 10.1038/sj.cgt.7700547View ArticlePubMedGoogle Scholar
- Nemunaitis J: Live viruses in cancer treatment. Oncology (Williston Park) 2002, 16: 1483-1492. discussion 1495–1487Google Scholar
- Lachmann R: Herpes simplex virus-based vectors. Int J Exp Pathol 2004, 85: 177-190. 10.1111/j.0959-9673.2004.00383.xPubMed CentralView ArticlePubMedGoogle Scholar
- Fu X, Nakamori M, Tao L, Amato R, Zhang X: Antitumor effects of two newly constructed oncolytic herpes simplex viruses against renal cell carcinoma. Int J Oncol 2007, 30: 1561-1567.PubMedGoogle Scholar
- Fu X, Tao L, Cai R, Prigge J, Zhang X: A mutant type 2 herpes simplex virus deleted for the protein kinase domain of the ICP10 gene is a potent oncolytic virus. Mol Ther 2006, 13: 882-890. 10.1016/j.ymthe.2006.02.007View ArticlePubMedGoogle Scholar
- Meseda CA, Schmeisser F, Pedersen R, Woerner A, Weir JP: DNA immunization with a herpes simplex virus 2 bacterial artificial chromosome. Virology 2004, 318: 420-428. 10.1016/j.virol.2003.09.033View ArticlePubMedGoogle Scholar
- Schmeisser F, Weir JP: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes. BMC Biotechnol 2007, 7: 22. 10.1186/1472-6750-7-22PubMed CentralView ArticlePubMedGoogle Scholar
- Jeyaretna DS, Kuroda T: Recent advances in the development of oncolytic HSV-1 vectors: 'arming' of HSV-1 vectors and application of bacterial artificial chromosome technology for their construction. Curr Opin Mol Ther 2007, 9: 447-466.PubMedGoogle Scholar
- Thomas DL, Fraser NW: HSV-1 therapy of primary tumors reduces the number of metastases in an immune-competent model of metastatic breast cancer. Mol Ther 2003, 8: 543-551. 10.1016/S1525-0016(03)00236-3View ArticlePubMedGoogle Scholar
- Todo T, Rabkin SD, Sundaresan P, Wu A, Meehan KR, Herscowitz HB, Martuza RL: Systemic antitumor immunity in experimental brain tumor therapy using a multimutated, replication-competent herpes simplex virus. Hum Gene Ther 1999, 10: 2741-2755. 10.1089/10430349950016483View ArticlePubMedGoogle Scholar
- Miller CG, Fraser NW: Requirement of an integrated immune response for successful neuroattenuated HSV-1 therapy in an intracranial metastatic melanoma model. Mol Ther 2003, 7: 741-747. 10.1016/S1525-0016(03)00120-5PubMed CentralView ArticlePubMedGoogle Scholar
- Nakamori M, Fu X, Rousseau R, Chen SY, Zhang X: Destruction of nonimmunogenic mammary tumor cells by a fusogenic oncolytic herpes simplex virus induces potent antitumor immunity. Mol Ther 2004, 9: 658-665. 10.1016/j.ymthe.2004.02.019View ArticlePubMedGoogle Scholar
- Li H, Dutuor A, Fu X, Zhang X: Induction of strong antitumor immunity by an HSV-2-based oncolytic virus in a murine mammary tumor model. J Gene Med 2007, 9: 161-169. 10.1002/jgm.1005View ArticlePubMedGoogle Scholar
- Hoffmann D, Bayer W, Wildner O: Local and distant immune-mediated control of colon cancer growth with fusogenic membrane glycoproteins in combination with viral oncolysis. Hum Gene Ther 2007, 18: 435-450. 10.1089/hum.2006.185View ArticlePubMedGoogle Scholar
- Kemeny N, Brown K, Covey A, Kim T, Bhargava A, Brody L, Guilfoyle B, Haag NP, Karrasch M, Glasschroeder B, et al.: Phase I, Open-Label, Dose-Escalating Study of a Genetically Engineered Herpes Simplex Virus, NV1020, in Subjects with Metastatic Colorectal Carcinoma to the Liver. Hum Gene Ther 2006, 17: 1214-1224. 10.1089/hum.2006.17.1214View ArticlePubMedGoogle Scholar
- Miller CG, Fraser NW: Role of the immune response during neuro-attenuated herpes simplex virus-mediated tumor destruction in a murine intracranial melanoma model. Cancer Res 2000, 60: 5714-5722.PubMedGoogle Scholar
- Cozzi PJ, Burke PB, Bhargav A, Heston WD, Huryk B, Scardino PT, Fong Y: Oncolytic viral gene therapy for prostate cancer using two attenuated, replication-competent, genetically engineered herpes simplex viruses. Prostate 2002, 53: 95-100. 10.1002/pros.10138View ArticlePubMedGoogle Scholar
- Cozzi PJ, Malhotra S, McAuliffe P, Kooby DA, Federoff HJ, Huryk B, Johnson P, Scardino PT, Heston WD, Fong Y: Intravesical oncolytic viral therapy using attenuated, replication-competent herpes simplex viruses G207 and Nv1020 is effective in the treatment of bladder cancer in an orthotopic syngeneic model. Faseb J 2001, 15: 1306-1308.PubMedGoogle Scholar
- Gutermann A, Mayer E, Von Dehn-Rothfelser K, Breidenstein C, Weber M, Muench M, Gungor D, Suehnel J, Moebius U, Lechmann M: Efficacy of oncolytic herpesvirus NV1020 can be enhanced by combination with chemotherapeutics in colon carcinoma cells. Hum Gene Ther 2006, 17: 1241-1253. 10.1089/hum.2006.17.1241View ArticlePubMedGoogle Scholar
- McAuliffe PF, Jarnagin WR, Johnson P, Delman KA, Federoff H, Fong Y: Effective treatment of pancreatic tumors with two multimutated herpes simplex oncolytic viruses. J Gastrointest Surg 2000, 4: 580-588. 10.1016/S1091-255X(00)80106-7View ArticlePubMedGoogle Scholar
- Advani SJ, Chung SM, Yan SY, Gillespie GY, Markert JM, Whitley RJ, Roizman B, Weichselbaum RR: Replication-competent, nonneuroinvasive genetically engineered herpes virus is highly effective in the treatment of therapy-resistant experimental human tumors. Cancer Res 1999, 59: 2055-2058.PubMedGoogle Scholar
- Meignier B, Longnecker R, Roizman B: In vivo behavior of genetically engineered herpes simplex viruses R7017 and R7020: construction and evaluation in rodents. J Infect Dis 1988, 158: 602-614.View ArticlePubMedGoogle Scholar
- Meignier B, Martin B, Whitley RJ, Roizman B: In vivo behavior of genetically engineered herpes simplex viruses R7017 and R7020. II. Studies in immunocompetent and immunosuppressed owl monkeys (Aotus trivirgatus). J Infect Dis 1990, 162: 313-321.View ArticlePubMedGoogle Scholar
- Cadoz M, Seigneurin JM, Mallaret MR, Baccard C, Morand P: Phase I trial of R7020: A live attenuated recombinant herpes simplex virus (HSV) candidate vaccine. In Program and Abstacts of the 32nd Interscience Conference on Antimicrobial Agents and Chemotherapy. American Society of Microbiology, Washington, D.C; 1992.Google Scholar
- Chou J, Kern ER, Whitley RJ, Roizman B: Mapping of herpes simplex virus-1 neurovirulence to gamma 134.5, a gene nonessential for growth in culture. Science 1990, 250: 1262-1266. 10.1126/science.2173860View ArticlePubMedGoogle Scholar
- Chou J, Roizman B: The gamma 1(34.5) gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shutoff of protein synthesis characteristic of programed cell death in neuronal cells. Proc Natl Acad Sci USA 1992, 89: 3266-3270. 10.1073/pnas.89.8.3266PubMed CentralView ArticlePubMedGoogle Scholar
- Kramm CM, Chase M, Herrlinger U, Jacobs A, Pechan PA, Rainov NG, Sena-Esteves M, Aghi M, Barnett FH, Chiocca EA, Breakefield XO: Therapeutic efficiency and safety of a second-generation replication-conditional HSV1 vector for brain tumor gene therapy. Hum Gene Ther 1997, 8: 2057-2068. 10.1089/hum.1997.8.17-2057View ArticlePubMedGoogle Scholar
- Bzik DJ, Fox BA, DeLuca NA, Person S: Nucleotide sequence specifying the glycoprotein gene, gB, of herpes simplex virus type 1. Virology 1984, 133: 301-314. 10.1016/0042-6822(84)90397-0View ArticlePubMedGoogle Scholar
- Pellett PE, Kousoulas KG, Pereira L, Roizman B: Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. J Virol 1985, 53: 243-253.PubMed CentralPubMedGoogle Scholar
- Bzik DJ, Fox BA, DeLuca NA, Person S: Nucleotide sequence of a region of the herpes simplex virus type 1 gB glycoprotein gene: mutations affecting rate of virus entry and cell fusion. Virology 1984, 137: 185-190. 10.1016/0042-6822(84)90022-9View ArticlePubMedGoogle Scholar
- Bond VC, Person S: Fine structure physical map locations of alterations that affect cell fusion in herpes simplex virus type 1. Virology 1984, 132: 368-376. 10.1016/0042-6822(84)90042-4View ArticlePubMedGoogle Scholar
- Debroy C, Pederson N, Person S: Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion. Virology 1985, 145: 36-48. 10.1016/0042-6822(85)90199-0View ArticlePubMedGoogle Scholar
- Foster TP, Chouljenko VN, Kousoulas KG: Functional and Physical Interactions of the Herpes Simplex Virus Type-1 (HSV-1) UL20 Membrane Protein with Glycoprotein K (gK). J Virol 2008, in press.Google Scholar
- Foster TP, Melancon JM, Olivier TL, Kousoulas KG: Herpes simplex virus type 1 glycoprotein K and the UL20 protein are interdependent for intracellular trafficking and trans-Golgi network localization. J Virol 2004, 78: 13262-13277. 10.1128/JVI.78.23.13262-13277.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Israyelyan AH, Melancon JM, Lomax LG, Sehgal I, Leuschner C, Kearney MT, Chouljenko VN, Baghian A, Kousoulas KG: Effective treatment of human breast tumor in a mouse xenograft model with herpes simplex virus type 1 specifying the NV1020 genomic deletion and the gBsyn3 syncytial mutation enabling high viral replication and spread in breast cancer cells. Hum Gene Ther 2007, 18: 457-473. 10.1089/hum.2006.145View ArticlePubMedGoogle Scholar
- Manservigi R, Spear PG, Buchan A: Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins. Proc Natl Acad Sci USA 1977, 74: 3913-3917. 10.1073/pnas.74.9.3913PubMed CentralView ArticlePubMedGoogle Scholar
- Tischer BK, von Einem J, Kaufer B, Osterrieder N: Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. Biotechniques 2006, 40: 191-197.View ArticlePubMedGoogle Scholar
- Aslakson CJ, Miller FR: Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res 1992, 52: 1399-1405.PubMedGoogle Scholar
- Kastrukoff LF, Lau AS, Puterman ML: Genetics of natural resistance to herpes simplex virus type 1 latent infection of the peripheral nervous system in mice. J Gen Virol 1986,67(Pt 4):613-621. 10.1099/0022-1317-67-4-613View ArticlePubMedGoogle Scholar
- Lopez C: Genetics of natural resistance to herpesvirus infections in mice. Nature 1975, 258: 152-153. 10.1038/258152a0View ArticlePubMedGoogle Scholar
- Shukla D, Dal Canto MC, Rowe CL, Spear PG: Striking similarity of murine nectin-1alpha to human nectin-1alpha (HveC) in sequence and activity as a glycoprotein D receptor for alphaherpesvirus entry. J Virol 2000, 74: 11773-11781. 10.1128/JVI.74.24.11773-11781.2000PubMed CentralView ArticlePubMedGoogle Scholar
- Even DL, Henley AM, Geraghty RJ: The requirements for herpes simplex virus type 1 cell-cell spread via nectin-1 parallel those for virus entry. Virus Res 2006, 119: 195-207. 10.1016/j.virusres.2006.01.012View ArticlePubMedGoogle Scholar
- Reif AE: Some key problems for success of classical immunotherapy. In Immunity to Cancer. Edited by: Reif AE, Mitchell EMS. New York: Academic Press; 1985:3-16.Google Scholar
- Miller FR: Models of progression spanning preneoplasia and metastasis: the human MCF10neoT.Tgn series and a panel of mouse mammary tumors subpopulations. In Mammary tumor cell cycle, Differentiation, and Metastasis. Edited by: Dickson MELRB. Boston: Kluwer Academic; 1996:243-263.View ArticleGoogle Scholar
- Pulaski BA, Ostrand-Rosenberg S: Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res 1998, 58: 1486-1493.PubMedGoogle Scholar
- Foster TP, Alvarez X, Kousoulas KG: Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localization of gK but not gK-mediated cell-to-cell fusion. J Virol 2003, 77: 499-510. 10.1128/JVI.77.1.499-510.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Foster TP, Rybachuk GV, Kousoulas KG: Glycoprotein K specified by herpes simplex virus type 1 is expressed on virions as a Golgi complex-dependent glycosylated species and functions in virion entry. J Virol 2001, 75: 12431-12438. 10.1128/JVI.75.24.12431-12438.2001PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.