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Molecular characterization and impacts of a strain of Grapevine leafroll-associated virus 2 causing asymptomatic infection in a wine grape cultivar

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Abstract

Background

Grapevine leafroll (GLD) is considered as the most economically important virus disease affecting wine grapes (Vitis vinifera L.) in many grapevine-growing regions. GLD produces distinct symptoms in red- and white-berried cultivars. In this study, we determined the complete genome sequence of an asymptomatic strain of Grapevine leafroll-associated virus 2 (GLRaV-2) and studied its impacts on fruit yield and berry quality attributes in an own-rooted, red-berried wine grape cultivar.

Findings

The complete genome of GLRaV-2 obtained from a red-berried wine grape cultivar Sangiovese, designated as GLRaV-2-SG, was determined to be 16,474 nucleotides in length. In pairwise comparisons, using complete genome sequences of GLRaV-2 strains available in GenBank, GLRaV-2-SG was more closely related to GLRaV-2-OR1 from Oregon, USA, and GLRaV-2-93/955 from South Africa, and distantly related to GLRaV-2-BD from Italy and GLRaV-2-RG from USA. Fruit yield estimates and berry quality analysis at the time of commercial harvest indicated that GLRaV-2-SG had little impact on fruit yield and total soluble solids, juice pH and total anthocyanins of berry skin.

Conclusions

Because so little is known about the effects of asymptomatic virus infections in wine grapes, this study expanded our knowledge of the occurrence and impacts of GLRaV-2 causing asymptomatic infections. Our results indicated that an asymptomatic strain of GLRaV-2 may not cause significant effects to overall fruit yield and berry quality in own-rooted vines, but can affect its host in more subtle ways. Since disease symptoms are not apparent, relying on visual symptoms during disease surveys may result in the escape of asymptomatic strains of GLRaV-2. Thus, it is necessary to use appropriate diagnostic assays for reliable detection of viruses causing asymptomatic infections.

Findings

Diseases caused by plant viruses are one of the major factors limiting sustainable production of wine grapes (Vitis vinifera L.) in many grapevine-growing regions [1, 2]. Among them, grapevine leafroll (GLD) is considered as the most economically important disease [3]. GLD produces distinct symptoms in red- and white-berried cultivars [4] and is known to affect vine vigor, fruit yield and berry quality in both grafted and own-rooted plants [59]. A number of genetically distinct viruses, designated as grapevine leafroll-associated viruses (GLRaVs) and numbered serially as GLRaV-1, -2, -3, etc. in the order of their documentation, have been reported in grapevines affected with GLD [10]. All GLRaVs belong to the family Closteroviridae, with majority of them assigned to the genus Ampelovirus. In contrast, GLRaV-2 belongs to the genus Closterovirus and GLRaV-7 is assigned to the proposed genus Velarivirus. GLRaV-8 is no longer considered as a valid species in the family Closteroviridae, since the reported sequence was from the grapevine genome [10].

In addition to distinct taxonomic and genomic characteristics that set apart GLRaV-2 from other GLRaVs, the virus is known to occur as divergent molecular variants segregating into six distinct lineages [11]. GLRaV-2 variants have been implicated in a wide range of pathological properties, including leafroll, graft union-incompatibility on sensitive rootstocks, young vine decline and rootstock stem lesion disease [1217]. A recent study indicated correlation between variants of GLRaV-2 belonging to distinct phylogenetic lineages and their pathological properties [16]. Most of these observations, however, were made with grafted vines using specific rootstocks. In contrast, the behavior of GLRaV-2 variants in own-rooted vines is not well studied.

In a recent study, molecular diversity of field isolates of GLRaV-2 collected from commercial vineyards in the Pacific Northwest region of the U.S. was reported [11]. Although most of these isolates were collected from own-rooted, red and white--berried wine grape cultivars showing GLD symptoms, some isolates were obtained from own-rooted Sangiovese vines showing no apparent visual symptoms. In this study, we determined the complete genome sequence of a GLRaV-2 isolate, designated as GLRaV-2-SG, causing asymptomatic infection in cv. Sangiovese, analyzed its genome characteristics with respect to other characterized GLRaV-2 strains and studied its impact on fruit yield and berry quality attributes.

Plant material was collected from Sangiovese vines planted in 1997 in a commercial vineyard. The name and location of this block was withheld to maintain grower confidentiality. Scrapings of cambial tissues from hardwood cuttings, obtained from a single grapevine tested positive only for GLRaV-2 ([11] Unpublished results) were used for isolating genomic-length, double-stranded (ds) RNA as described by Valverde et al. [18]. The dsRNA-enriched preparation was subjected to DNase I (Invitrogen, Carlsbad, CA, USA) treatment and subsequently used as a template for amplification of different portions of the virus genome.

Consensus primers were designed based on alignment of nucleotide sequence of the complete genome of GLRaV-2 strains available in GenBank (Additional file 1: Table S1). One step-single tube RT-PCR assays were carried out to amplify overlapping segments of the virus genome using the following conditions: cDNA synthesis at 50°C for 45 min followed by initial denaturation at 95°C for 5 min, 40 consecutive cycles of 95°C for 30 s, 57°C for 60 s and 72°C for 90 s per cycle, and a final extension step for 10 min at 72°C. The amplicons were cloned into pCR2.1 vector (Life Technologies, Carlsbad, CA, USA) and three independent clones per amplicon were sequenced in both orientations. Wherever necessary, additional clones were sequenced to resolve nucleotide sequence ambiguities. The 5′ and 3′ terminal sequence of GLRaV-2 was determined using the FirstChoice® RLM-RACE Kit (Ambion, Austin, TX, USA) following the manufacturer's instructions.

GLRaV-2-specific primers (SG5p: 5′-GAGCGACTGAGCAGGGAAGGGTGG-3′ and SG3p: 5′-GAGCGACTGAGCAGGGAAGGGTGG-3′) were used in combination with primers provided in the RACE kit to amplify approximately 150 bp and 250 bp fragments corresponding to the 5′ and 3′ termini, respectively. Amplicons were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer’s instructions, cloned and 15 recombinant clones per amplicon sequenced in both orientations. Nucleotide sequence annotation, ORF prediction and sequence assembly were carried out using Vector NTI Advance 11 program (Life Technologies, Carlsbad, CA, USA). Multiple alignments of nucleotide and amino acid sequences and construction of Neighbor-Joining (NJ) phylogenetic trees were performed using ClustalW with default settings from MEGA5 software [19].

To study impacts of GLRaV-2-SG, five pairs of own-rooted Sangiovese vines, with each pair consisting of GLRaV-2-SG-infected and non-infected grapevines located adjacent to each other in a given row, were selected to minimize error in experimental results due to variations in soil and other growing conditions. At the time of commercial harvest in October 2012, berry clusters from individual grapevines were counted and their combined weight measured. Juice extracted from 100 berries collected randomly from individual GLRaV-2-SG-infected and non-infected vines was used separately to measure total soluble solids (TSS), pH, titratable acidity (TA) and total anthocyanins as described previously [20]. Statistical significance of the data between GLRaV-2-SG-infected and non-infected vines was analyzed by One-way ANOVA. Data are presented as mean ± SE (standard error). The minimum accepted P value for significance was 0.05.

Using the dsRNA-enriched preparation from a single asymptomatic Sangiovese vine as a template (Additional file 1: Figure S1), we amplified overlapping cDNA fragments spanning the entire genome of the virus with primers listed in Additional file 1: Table S1. By cloning and sequencing of amplicons, we assembled the 16,474 nucleotide (nt) genome of GLRaV-2-SG isolate (GenBank: KF220376). Our analysis indicated that the organization of GLRaV-2-SG genome is similar to other characterized strains of GLRaV-2 and encodes nine putative ORFs with 106 nt long 5′ non-translated region (NTR) and 206 nt long 3′NTR (Figure 1 and Table 1). A pairwise comparison of the complete genome sequence of GLRaV-2-SG with other GLRaV-2 sequences available in GenBank indicated that GLRaV-2-SG shares 85% sequence identity with GLRaV-2-93/955 (NC007448) from South Africa and GLRaV-2-OR1 (FJ436234) from Oregon, USA, and 72% identity with GLRaV-2-BD (DQ286725) from Italy and GLRaV-2-RG (NC004724) from USA. A pairwise comparison of individual ORFs encoded by GLRaV-2-SG with corresponding sequences from other isolates are shown in Table 1. The data indicated greater than 86% amino acid (aa) sequence identities among all ORFs, except p19 that showed 83 and 84% aa identity with corresponding sequence of isolates OR1 and 93/955 and 71% identity with BD and RG isolates. The regions with the lowest nt sequence identity were identified within ORF1a between nt 950–1193, nt 1575–2196 and nt 5769–6828. GLRaV-2-SG shared 78-89% aa sequence identity with other GLRaV-2 isolates (OR1, 93/955, BD and RG) (Figure 1) in these three regions resulting in significant aa sequence differences (data not shown). Since the aa identity values of the ORF1b, p6, heat-shock protein 70 homolog (HSP70h), p63, coat protein (CP), minor coat protein (CPm) and p24, with the exception of p19, fall within the 10-15% identity range, GLRaV-2-SG may be considered as a distinct strain of GLRaV-2 based on the criteria established for sequence identity threshold values suggested for demarcation of species and their strains of GLRaVs [10].

Figure 1
figure1

Sliding-window Simplot graph showing genome-wide comparison of Grapevine leafroll-associated virus 2, strain SG (GLRaV-2-SG) with other strains of GLRaV-2 (OR1, 93/955, BD and RG; GenBank: FJ436234; NC007448; DQ286725; NC004724). The Simplot was generated using multiple sequence alignment of all strains with a window size of 200 nt and a step size of 20 nts. Diagrammatic representation of the GLRaV-2 genome with location of 9 open reading frames (ORFs) from 5′ to 3′ are shown above the graph. See Martelli et al. [10] for details of genome organization of the virus.

Table 1 Percent (%) nucleotide (nt) and amino acid (aa) identities between non-translated regions, protein-coding genes and complete genome of SG strain Grapevine leafroll-associated virus 2 (GLRaV-2-SG; KF220376) and other strains of GLRaV-2 (OR1, 93/955, BD and RG; GenBank: FJ436234; NC007448; DQ286725; NC004724)

Fruit yield measurements at the time of commercial harvest indicated no significant differences in the number of clusters and cumulative weight of fruits between GLRaV-2-SG-positive and negative Sangiovese vines (Table 2). Analysis of fruit quality parameters indicated that TSS (measured as °Brix) and TA was reduced by 3.2% and 3.8%, respectively, in berries from vines infected with GLRaV-2-SG, compared to berries from virus-negative vines. Likewise, no significant differences were observed in berry juice pH between virus-positive and virus-negative vines. Total extractable anthocyanins of berries were less by 17.7% in virus-positive vines compared to adjacent virus-negative vines. However, the difference was considered statistically insignificant due to high standard deviation that is more than three quarters of the value of the difference between virus-positive and negative samples. Additional studies involving more sampling size are in progress to resolve this discrepancy. Overall, these results suggest that GLRaV-2-SG showed no significant negative impacts on fruit yield and berry quality in infected vines.

Table 2 Impact of SG strain Grapevine leafroll-associated virus 2 (GLRaV-2-SG) on fruit yield and berry quality attributes

In conclusion, this study described the molecular characterization of a naturally occurring GLRaV-2 strain causing asymptomatic infection in an own-rooted wine grape cultivar Sangiovese. GLRaV-2-SG infection with no visually apparent symptoms caused insignificant impacts on yield and fruit quality. A recent study found asymptomatic infections of GLRaV-7 in wine grape cultivars, such as Pinot Noir and Cabernet Franc [21]. Although the mechanism(s) behind asymptomatic infection of GLRaVs has yet to be elucidated, our results, together with observations reported by Al Rwahnih et al. [21] for GLRaV-7, support the possibility of the occurrence of GLRaVs causing asymptomatic infections in wine grape cultivars. Further research is in progress to study if the asymptomatic phenotype of GLRaV-2-SG is an intrinsic property of the virus or dependent on specific virus-cultivar interactions. Regardless, this study provides further evidence that visual symptoms alone are not a reliable criterion for the field diagnosis of GLD and it is more likely that GLRaVs causing asymptomatic infections can be disseminated via vegetative propagation. Thus, rigorous testing of grapevines is vital to prevent escape of asymptomatic strains through the supply of planting materials to nurseries and grape growers.

Abbreviations

RdRp:

RNA-dependent RNA polymerase

HSP70h:

Heat-shock protein 70 homolog

CP:

Coat protein

CPm:

Minor coat protein.

References

  1. 1.

    Martelli GP, Boudon Padieu E, International Centre for Advanced Mediterranean Agronomic Studies: Directory of infectious diseases of grapevines. Options Méditerr Ser B Stud Res 2006, 55: 59-75.

  2. 2.

    Oliver JE, Fuchs M: Tolerance and resistance to viruses and their vectors in vitis sp.: a virologist’s perspective of the literature. Am J Enol Vitic 2011, 62: 438-451. 10.5344/ajev.2011.11036

  3. 3.

    Martelli GP: Major graft-transmissible diseases of grapevines: nature, diagnosis, and sanitation. Am J Enol Vitic 2000, 51: 42-48.

  4. 4.

    Rayapati AN, O'Neil S, Walsh D: Grapevine Leafroll Disease. WSU Extension Bulletin EB2027E; 2008:20. http://cru.cahe.wsu.edu/CEPublications/eb2027e/eb2027e.pdf

  5. 5.

    Golino DA, Wolpert J, Sim ST, Benz J, Anderson M, Rowhani A 16th Meeting of International Council for the Study of Virus and Virus like Diseases of Grapevine (ICVG). Virus Effects on Vine Growth and Fruit Components of Three California ‘heritage’ Clones of Cabernet Sauvignon 2009, p243.

  6. 6.

    Lee J, Martin RR: Influence of grapevine leafroll associated viruses (GLRaV-2 and −3) on the fruit composition of Oregon Vitis vinifera L. cv. Pinot noir: Phenolics . Food Chem 2009, 112: 889-896. 10.1016/j.foodchem.2008.06.065

  7. 7.

    Lee J, Keller KE, Rennaker C, Martin RR: Influence of grapevine leafroll associated viruses (GLRaV-2 and −3) on the fruit composition of Oregon Vitis vinifera L. cv. Pinot noir : Free amino acids, sugars, and organic acids. Food Chem 2009, 117: 99-105. 10.1016/j.foodchem.2009.03.082

  8. 8.

    Mannini F, Mollo A, Credi R: Field performance and wine quality modification in a clone of Vitis vinifera cv. Dolcetto after GLRaV-3 elimination. Am J Enol Vitic 2012, 63: 144-147. 10.5344/ajev.2011.11020

  9. 9.

    Alabi OJ, Gutha LR, Casassa LF, Harbertson J, Mirales M, Beaver CW, Davenport J, Naidu RA 63rd American Society for Enology and Viticulture National Conference. Impacts of grapevine leafroll disease on own- rooted wine grape cultivar in cool climate conditions 2012, 69-70.

  10. 10.

    Martelli GP, Abou Ghanem-Sabanadzovic N, Agranowsky AA, Al Rawhanih M, Dolja VV, Dovas CI, Fuchs M, Gugerli P, Hu JS, Jelkmann W, Katis N, Maliogka VI, Melzer MJ, Menzel W, Minafra A, Rott ME, Rowhani A, Sabanadzovic S, Saldarelli P: Taxonomic revision of the family Closteroviridae with special reference to the grapevine leafroll-associated member of the genus Ampelovirus and the putative species unassigned to the family. J Plant Pathol 2012, 94: 7-19.

  11. 11.

    Jarugula S, Alabi OJ, Martin RR, Naidu RA: Genetic variability of natural populations of Grapevine leafroll-associated virus 2 in Pacific Northwest vineyards. Phytopathol 2010, 100: 698-707. 10.1094/PHYTO-100-7-0698

  12. 12.

    Greif C, Garau R, Boscia D, Prota VA, Fiori M, Bass P, Walter B, Prota U: The relationship of grapevine leafroll-associated closterovirus 2 with a graft incompatibility condition of grapevines. Phytopathol 1995, 34: 167-173.

  13. 13.

    Uyemoto JK, Rowhani A, Luvisi D, Randy Krag C: New closterovirus in Redglobe grape causes decline of grafted plants. Calif Agric 2001, 55: 28-31.

  14. 14.

    Bonfiglioli R, Edwards F, Hoskins N, Pantaleo A: Graft incompatibility syndrome in New Zealand Merlot vines involves another possible variant of GLRaV-2. Aust NZ Grapegrower Winemaker 2003, 476: 50-54.

  15. 15.

    Bertazzon N, Angelini E: Advances in the detection of Grapevine leafroll-associated virus 2 variants. J Plant Pathol 2004, 86: 283-290.

  16. 16.

    Bertazzon N, Borgo M, Vanin S, Angelini E: Genetic variability and pathological properties of Grapevine Leafroll-associated Virus 2 isolates. Eur J Plant Pathol 2010, 127: 185-197. 10.1007/s10658-010-9583-3

  17. 17.

    Alkowni R, Zhang YP, Rowhani A, Uyemoto JK, Minafra A: Biological, molecular, and serological studies of a novel strain of Grapevine leafroll-associated virus 2. Virus Genes 2011, 43: 102-110. 10.1007/s11262-011-0607-7

  18. 18.

    Valverde R, Nameth ST, Jordan RL: Analysis of double-stranded RNA for plant virus diagnosis. Plant Dis 1990, 74: 255-258.

  19. 19.

    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28: 2731-2739. 10.1093/molbev/msr121

  20. 20.

    Poojari S, Alabi OJ, Fofanov VY, Naidu RA: Next-generation sequencing of grapevines ( Vitis vinifera ) showing redleaf symptoms implicates a leafhopper-transmissible DNA virus with novel evolutionary lineage in the family Geminiviridae . PLoS ONE 2013, 8: e64194. doi:10.1371/journal.pone.0064194 10.1371/journal.pone.0064194

  21. 21.

    Al Rwahnih M, Dolja VV, Daubert S, Koonin EV, Rowhani A: Genomic and biological analysis of Grapevine leafroll-associated virus 7 reveals a possible new genus within the family Closteroviridae . Virus Res 2012, 163: 302-309. 10.1016/j.virusres.2011.10.018

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Acknowledgements

This study was funded, in part, by the Agricultural Research Center of the College of Agricultural, Human, and Natural Resource Sciences, Washington State University; Washington Wine Commission’s Wine Advisory Committee; USDA-NIFA Specialty Crop Research Initiative (2009-51181- 06027) and USDA-Northwest Center for Small Fruits Research. PPNS number 0635, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center Project Number WNPO 0616, Washington State University, Pullman 99164–6240. We thank Dr. Jim Harbertson and Ms. Maria Mireles for technical assistance in biochemical analysis of berry samples, and Drs. Richard Larsen and Scott Adkins for reviewing the manuscript.

Author information

Correspondence to Rayapati A Naidu.

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Competing interests

The authors have declared that no competing interests exist.

Authors’ contributions

Conceived and designed the experiments: RAN, SP, OJA. Performed the experiments: SP, OJA. Analyzed the data: SP, OJA, RAN. Contributed reagents/materials/analysis tools: RAN. Wrote the paper: SP, OJA, RAN. All authors read and approved the final manuscript.

Electronic supplementary material

Additional file 1: Figure S1: Electrophoretic pattern of dsRNA-enriched preparation of Grapevine leafroll-associated virus 2, strain SG (GLRaV-2-SG) obtained from cv. Sangiovese (lane 2). dsRNA-enriched preparation from Nicotiana benthamiana infected with Potato virus Y (PVY) and Cucumber mosaic virus (CMV) (lane 1) were included as markers. Expected size of dsRNAs is shown by arrows. M represents 1Kb Plus DNA ladder (Life Technologies, Carlsbad, CA, USA). Table S1. Nucleotide sequences of forward (F) and reverse (R) primers used for amplification of the complete genome of GLRaV-2-SG. Position refers to the location of primer sequences on the GLRaV-2-SG genome. (PDF 24 KB)

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Keywords

  • Total Soluble Solid
  • Fruit Yield
  • Asymptomatic Infection
  • Total Anthocyanin
  • Wine Grape