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A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures
Virology Journal volume 16, Article number: 159 (2019)
Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety level-4 (BSL-4) laboratories, which are restricted to trained and authorized individuals.
To overcome this limitation, we developed a live-cell imaging system to visualize MARV nucleocapsid-like structures using fluorescence-conjugated viral proteins, which can be conducted outside BSL-4 laboratories.
Our experiments revealed that nucleocapsid-like structures have similar transport characteristics to those of nucleocapsids observed in MARV-infected cells, both of which are mediated by actin polymerization.
We developed a non-infectious live cell imaging system to visualize intracellular transport of MARV nucleocapsid-like structures. This system provides a safe platform to evaluate antiviral drugs that inhibit MARV nucleocapsid transport.
Marburg virus (MARV), together with Ebola virus (EBOV), belongs to the family Filoviridae, and has a roughly 19 kb non-segmented, single-stranded, negative-sense RNA genome. It can cause severe hemorrhagic fever with high fatality rates. MARV epidemics have occasionally been reported in Central Africa, with the largest one, having a 90% fatality rate, being reported in Angola between 2004 and 2005 . MARV infection was also reported outside Central Africa, such as in Germany, South Africa, Russia, and USA . Currently, there are no approved vaccines or antiviral therapeutics available to prevent or treat MARV infection. Therefore, understanding the interplay between viral and host proteins during MARV replication is necessary to establish countermeasures for the diseases. For example, revealing the mechanisms for the assembly and transport of nucleocapsids, which are responsible for the transcription and replication of the viral genome, might contribute to the development of new therapeutic options.
The main nucleocapsid protein of MARV is NP, which is responsible for the encapsidation of single-stranded viral genomic RNA [3, 4]. In addition to NP, MARV nucleocapsids also contain the minor matrix protein VP24 and the polymerase cofactor VP35, both of which are essential structural elements that directly interact with NP to build a helical nucleocapsid approximately 900 nm in length and 50 nm in diameter [3, 5, 6]. Furthermore, the viral polymerase L and the transcription factor VP30 are also associated with the nucleocapsid [7, 8]. The core complex of the nucleocapsid, formed by the NP, VP35, and VP24 proteins together with the viral RNA, is defined as a nucleocapsid-like structure (NCLS, Fig. 1a). In MARV- and EBOV-infected cells, immunofluorescence microscopy, as well as live-cell imaging, have been used to visualize and analyze nucleocapsids by fluorescently labeling the nucleocapsid proteins [9,10,11,12,13]. According to these reports, nucleocapsid formation occurs in the perinuclear inclusion bodies, following which they are transported into the cytoplasm and redistributed prior to budding through the cell surface. The velocity of nucleocapsid movement inside cells ranges from 100 nm/s to 500 nm/s in MARV- or EBOV-infection [9, 10]. Application of specific cytoskeleton inhibitors revealed that the transport of MARV nucleocapsids was dependent on actin polymerization. Viral matrix protein VP40, which is a peripheral membrane protein and plays a pivotal role in filamentous virion formation, is essential for the recruitment of nucleocapsids to the cell periphery and for their incorporation into progeny virions [3, 14,15,16]. The surface glycoprotein GP, which is an integral membrane protein and is responsible for cell entry, forms the filamentous virions together with nucleocapsid and VP40 (Fig. 1b) [11, 17,18,19].
Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of living organisms. In addition to immunofluorescence microscopy, live-cell imaging microscopy has been utilized to visualize the localization of viral proteins, and interactions between viral and host proteins in various virus-infected cells [11, 20,21,22,23]. However, because of its high pathogenicity, MARV must be handled under the highest biosafety conditions [biosafety level 4 (BSL-4)], which complicates and delays research using live-cell imaging . In this study, we developed a safe, live-cell imaging system, following a previously established method for EBOV , to visualize MARV nucleocapsid-like structures (NCLSs) in cells expressing viral proteins, outside of BSL-4 laboratories. By using this live-cell imaging system, we were able to analyze interactions between NCLSs and the cellular cytoskeleton, as well as intracellular transport of NCLSs.
Materials and methods
Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol) Fetal bovine serum (FBS, PAN Biotech), 5 mM L-glutamine (Q; Life Technologies), 50 U/mL penicillin, and 50 μg/mL streptomycin (PS; Life Technologies).
Plasmids and transfection
Plasmids encoding the MARV structural proteins (NP, VP35, VP24, L, VP40 and GP): pCAGGS-NP, pCAGGS-VP35, pCAGGS-VP24, pCAGGS-L, pCAGGS-VP40, and pCAGGS-GP, and a MARV minigenome-expressing plasmid which encodes a Renilla luciferase were used [26, 27]. The plasmid pCAGGS-VP30-GFP, coding for the green fluorescent protein-VP30 fusion protein, was produced as previously described . The transfection was performed in 50 μL Opti-MEM without phenol red (Life Technologies) using TranSIT (Mirus) according to the manufacturer’s instructions.
Live cell imaging microscopy
A total of 2 × 104 Huh-7 cells were seeded onto a μ-Slide 4 well (ibidi) and cultivated in DMEM/PS/Q with 10% FBS. Each well was transfected with the following plasmids, encoding all MARV structural proteins: (250 ng of pCAGGS-NP, 50 ng of pCAGGS-VP35, 125 ng of pCAGGS-VP30-GFP, 50 ng of pCAGGS-VP24, 500 ng of pCAGGS-L, 125 ng of pCAGGS-VP40 and 125 ng of pCAGGS-GP), together with a T7-driven, MARV minigenome-expressing plasmid, which encodes a Renilla luciferase, and a T7 polymerase-coding plasmid (pCAGGS-T7) [26, 27]. The inoculum was removed at 1 h post-transfection (p.t.), and 500 μL CO2-independent Leibovitz’s medium (Life Technologies) with PS/Q, non-essential amino acid solution, and 20% (vol/vol) FBS were added. Live-cell time-lapse experiments were recorded with a Nikon ECLIPSE TE2000-E using a 63× oil objective or a GE healthcare Delta Vision Elite using a 60× oil objective in biosafety level-2 laboratories.
Treatment of cells with cytoskeleton-modulating drugs
Cells were treated with 15 μM nocodazole (Sigma), 0.3 μM cytochalasin D (Sigma), or 0.15% dimethyl sulfoxide (DMSO, Sigma), following previous publication . The chemicals were added to the cell culture medium 3 h prior to observation.
Image processing and analysis
Acquired pictures and movie sequences were processed using the Fiji plugin “TrackMate” [28, 29]. We used LoG detector, which enables detection of the targeted signals to the greatest possible extent. Subsequently, a Simple LAP tracker was used to follow trajectories of the moving signals . The majority of the detected signals were VP30-derived movement without formation of NCLSs, which can be differentiated by shorter trajectory length and random movement pattern . To avoid contamination of the signals derived solely from VP30, we omitted the signals demonstrating trajectory length less than 1 μm and velocity less than 10 nm/s, because they do not conform to the typical transport pattern of NCLSs/nucleocapsids, which is characterized by long-distance directional movement [9, 10, 25]. Previously, we used manual tracking of signals, which primarily demonstrates trajectory length more than 5 μm, track duration over 30 s, and velocity of movement over 100 nm/s [9, 10, 25], although it was not optimized for quantitative analyses.
Establishment of a live-cell imaging system for MARV NCLSs transport
The Marburg virus virus-like particle (VLP) system, which models a complete, single infectious cycle, has been developed and used to analyze viral transcription and replication, as well as the budding processes [26, 27]. In this study, we attempted to visualize MARV NCLSs transport in Huh-7 cells, by using the MARV VLP system and based on the procedure established for EBOV . Cells were transfected with the plasmids encoding the VLP components as illustrated in Fig. 2a. We employed this VLP-based system in all subsequent experiments in this study.
At 18 h p.t., we detected a large number of moving signals with various shapes (Additional file 1: Movie S1). Over 1000 signals in the acquired movie sequences were analyzed. The representing sequence was expressed as the maximum intensity projection, in which the image projected maximum intensity of each time lapse. Among the signals, we focused on those signals showing long-distance and directional transport, which represent NCLSs trajectories . We detected trajectories with lengths ranging from 100 nm to 20 μm, with a mean length of 1.89 ± 0.91 μm (Fig. 2c). The direction of movement of each NCLS also varied, though the cause of this variation remains unclear. The speed of MARV NCLSs transport ranged from 10 nm/s to 500 nm/s, with a mean velocity of 167 ± 96 nm/s (Fig. 2d), which is comparable to the velocity of nucleocapsid transport in MARV-infected cells analyzed in BSL-4 laboratory (106 ± 96 nm/s at plasma membrane, 132 ± 59 nm/s inside filopodia, and 411 ± 87 nm/s in cytoplasm) . In summary, the movement characteristics of NCLSs in our system is similar to those of nucleocapsids in MARV-infected cells.
Actin polymerization is required for MARV NCLSs transport
In MARV-infected cells, the microtubule depolymerizing drug nocodazole does not alter the movement of nucleocapsids, whereas the actin depolymerizing drug cytochalasin D arrests it . To confirm the relevance of the live-cell imaging system we developed in this study, we analyzed NCLSs movement after treatment with cytoskeletal modulating drugs. Huh-7 cells were transfected with plasmids as described in Fig. 2a. The culture medium was replaced at 15 h p.t. with Leibovitz’s medium containing either 0.15% DMSO (control), 0.15 M nocodazole, or 0.3 μM cytochalasin D (Fig. 3a-d, Additional file 2: Movie S2, Additional file 3: Movie S3, Additional file 4: Movie S4) . After incubating the cells with cytoskeletal modulating drugs for 3 h, time-lapse images were acquired. Nocodazole treatment did not alter the trajectory length of NCLSs transport in comparison to the control, whereas cytochalasin D treatment induced immediate cessation of long-distance transport (Fig. 3e-g). The mean velocity of NCLSs transport in the control or nocodazole-treated cells was 188 ± 98 nm/s and 219 ± 99 nm/s, respectively (Fig. 3h). The mean velocity of nucleocapsid transport in MARV-infected cells treated with DMSO and nocodazole was 379 ± 61 nm/s and 334 ± 55 nm/s, respectively . A reduction in mean velocity is derived from the difference in the inclusion criteria for analysis, which was described in the Materials and Methods. Briefly, the trajectories of long-distance signals in the cytoplasmic region were analyzed in the previous reports [9, 25], whereas we applied software-based quantitative analysis, including the signals in the cytoplasm, plasma membrane, and inside filopodia, in the current study. On the other hand, only a few non-specific NCLSs movements were detectable in cytochalasin D-treated cells, with a mean velocity of 2.73 ± 23 nm/s (Fig. 3i-j). These results confirmed that NCLSs transport is dependent on actin polymerization, as well as the suitability of our assay to test candidate drugs which target intracellular transport of nucleocapsid.
In the present study, we developed a live-cell imaging system for cells expressing MARV proteins, which can be safely used without BSL-4 laboratories. Furthermore, we demonstrated the relevance of our system as a substitute for the analysis of nucleocapsids transport in MARV-infected cells.
We previously developed a live-cell imaging system visualizing intracellular transport of NCLSs in EBOV proteins-expressing cells . According to this, the transport characteristics of EBOV NCLSs, velocity and mediated by actin polymerization, are similar to those of nucleocapsids observed in EBOV-infected cells [10, 25]. In the previous reports, we manually measured and calculated the trajectory length and velocity of nucleocapsids and NCLSs transport [9, 10, 25]. To improve quantity of analysis for NCLSs transport, here we applied automatic detection of moving signals using Fiji plugin TrackMate . By adopting selection criteria, we collected sufficient moving signals except for Brownian like random movement. The calculated velocity of moving NCLSs is similar in both methods.
Currently, cellular factors involved in the nucleocapsid transport have not been fully understood. Although there are several actin-dependent motor proteins such as myosins, and actin-associated proteins necessary for polymerization, such as Arp2/3 and N-WASP, only Arp2/3 was known to be directly associated with Ebola virus nucleocapsid transport . The combined approach of gene silencing and inhibitor screening using the system we developed might represent a powerful tool to identify the key host factors for the intracellular transport of MARV nucleocapsids. Moreover, it is noteworthy that the technical approach developed here might be applicable to study the nucleocapsid transport of other mononegaviruses, as well as to characterize antivirals inhibiting nucleocapsid transport.
We developed a live-cell imaging system for cells expressing MARV proteins, which can be safely used without BSL-4 laboratories, and demonstrated the relevance of our system as a substitute for the analysis of nucleocapsids transport in MARV-infected cells. Our developed live-imaging system might contribute to study the nucleocapsid transport of other mononegaviruses, as well as to characterize antivirals inhibiting nucleocapsid transport.
Availability of data and materials
The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request.
Dulbecco’s Modified Eagle Medium
Fetal bovine serum
Penicillin and streptomycin
CDC: Chronology of Marburg Hemorrhagic Fever Outbreaks. 2014.
Prevention CfDCa: Outbreaks chronology: Marburg hemorrhagic fever. 2014.
Bharat TA, Noda T, Riches JD, Kraehling V, Kolesnikova L, Becker S, Kawaoka Y, Briggs JA. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. Proc Natl Acad Sci U S A. 2012;109:4275–80.
Muhlberger E, Lotfering B, Klenk HD, Becker S. Three of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomes. J Virol. 1998;72:8756–64.
Huang Y, Xu L, Sun Y, Nabel GJ. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell. 2002;10:307–16.
Watanabe S, Noda T, Kawaoka Y. Functional mapping of the nucleoprotein of Ebola virus. J Virol. 2006;80:3743–51.
Hartlieb B, Modrof J, Muhlberger E, Klenk HD, Becker S. Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide. J Biol Chem. 2003;278:41830–6.
Biedenkopf N, Hartlieb B, Hoenen T, Becker S. Phosphorylation of Ebola virus VP30 influences the composition of the viral nucleocapsid complex: impact on viral transcription and replication. J Biol Chem. 2013;288:11165–74.
Schudt G, Kolesnikova L, Dolnik O, Sodeik B, Becker S. Live-cell imaging of Marburg virus-infected cells uncovers actin-dependent transport of nucleocapsids over long distances. Proc Natl Acad Sci U S A. 2013;110:14402–7.
Schudt G, Dolnik O, Kolesnikova L, Biedenkopf N, Herwig A, Becker S. Transport of Ebolavirus Nucleocapsids is dependent on actin polymerization: live-cell imaging analysis of Ebolavirus-infected cells. J Infect Dis. 2015;212(Suppl 2):S160–6.
Becker S, Klenk HD, Muhlberger E. Intracellular transport and processing of the Marburg virus surface protein in vertebrate and insect cells. Virology. 1996;225:145–55.
Kolesnikova L, Bamberg S, Berghofer B, Becker S. The matrix protein of Marburg virus is transported to the plasma membrane along cellular membranes: exploiting the retrograde late endosomal pathway. J Virol. 2004;78:2382–93.
Kolesnikova L, Berghofer B, Bamberg S, Becker S. Multivesicular bodies as a platform for formation of the Marburg virus envelope. J Virol. 2004;78:12277–87.
Noda T, Watanabe S, Sagara H, Kawaoka Y. Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus. J Virol. 2007;81:3554–62.
Noda T, Sagara H, Suzuki E, Takada A, Kida H, Kawaoka Y. Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol. 2002;76:4855–65.
Dolnik O, Kolesnikova L, Stevermann L, Becker S. Tsg101 is recruited by a late domain of the nucleocapsid protein to support budding of Marburg virus-like particles. J Virol. 2010;84:7847–56.
Mittler E, Kolesnikova L, Strecker T, Garten W, Becker S. Role of the transmembrane domain of Marburg virus surface protein GP in assembly of the viral envelope. J Virol. 2007;81:3942–8.
Beniac DR, Booth TF. Structure of the Ebola virus glycoprotein spike within the virion envelope at 11 a resolution. Sci Rep. 2017;7:46374.
Booth TF, Rabb MJ, Beniac DR. How do filovirus filaments bend without breaking? Trends Microbiol. 2013;21:583–93.
Nanbo A, Watanabe S, Halfmann P, Kawaoka Y. The spatio-temporal distribution dynamics of Ebola virus proteins and RNA in infected cells. Sci Rep. 2013;3:1206.
Hoenen T, Shabman RS, Groseth A, Herwig A, Weber M, Schudt G, Dolnik O, Basler CF, Becker S, Feldmann H. Inclusion bodies are a site of ebolavirus replication. J Virol. 2012;86:11779–88.
Lakdawala SS, Wu Y, Wawrzusin P, Kabat J, Broadbent AJ, Lamirande EW, Fodor E, Altan-Bonnet N, Shroff H, Subbarao K. Influenza a virus assembly intermediates fuse in the cytoplasm. PLoS Pathog. 2014;10:e1003971.
Takamatsu Y, Kajikawa J, Muramoto Y, Nakano M, Noda T. Microtubule-dependent transport of arenavirus matrix protein demonstrated using live-cell imaging microscopy. Microscopy (Oxf). 2019.
Falzarano D, Geisbert TW, Feldmann H. Progress in filovirus vaccine development: evaluating the potential for clinical use. Expert Rev Vaccines. 2011;10:63–77.
Takamatsu Y, Kolesnikova L, Becker S. Ebola virus proteins NP, VP35, and VP24 are essential and sufficient to mediate nucleocapsid transport. Proc Natl Acad Sci U S A. 2018;115:1075–80.
Wenigenrath J, Kolesnikova L, Hoenen T, Mittler E, Becker S. Establishment and application of an infectious virus-like particle system for Marburg virus. J Gen Virol. 2010;91:1325–34.
Hoenen T, Groseth A, de Kok-Mercado F, Kuhn JH, Wahl-Jensen V. Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics systems for filoviruses and other negative stranded hemorrhagic fever viruses. Antivir Res. 2011;91:195–208.
Tinevez JY, Perry N, Schindelin J, Hoopes GM, Reynolds GD, Laplantine E, Bednarek SY, Shorte SL, Eliceiri KW. TrackMate: an open and extensible platform for single-particle tracking. Methods. 2017;115:80–90.
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676–82.
The authors are grateful to Gordian Schudt (Philipps University Marburg, Germany) and Asuka Nanbo (Nagasaki University, Japan) for fruitful discussion.
The work was supported by Japan Society for the Promotion of Science JSPS Grant number 18 J01631, 19 K16666, by the Joint Usage / Research Center on Tropical Disease, Institute of Tropical Medicine, and Nagasaki University (2019-Ippan-24) (to Y.T.), by AMED Research Program on Emerging and Re-emerging Infectious Diseases (18fk0108055h0002), by AMED Japanese Initiative for Progress of Research on Infectious Disease for global Epidemic (18fm0208003h0002), by MEXT Grant-in-Aid for Scientific Research on Innovative Area (19H04831), by JSPS Core-to-Core Program A, the Advanced Research Networks, by Grant for Joint Research Project of the Institute of Medical Science, University of Tokyo, by Joint Usage/Research Center program of Institute for Frontier Life and Medical Sciences Kyoto University, by the Daiichi Sankyo Foundation of Life Science, and by the Takeda Science Foundation (to T.N.), and by the Deutsche Forschungsgemeinschaft (DFG, German research foundation) Project number 197785619-SFB 1021 (to S.B.).
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Additional file 2 : Movie S2. Huh-7 cells were transfected with plasmids encoding NP, L, VP35, VP24, VP40, GP, Marburg virus specific minigenome, T7 polymerase, and VP30-GFP. The cells were treated at 15 h p.t. with 0.15% DMSO for 3 h. Time-lapse images of cells were recorded for 90 s; images were captured every 2 s.
Additional file 3 : Movie S3. Huh-7 cells were transfected with plasmids encoding NP, L, VP35, VP24, VP40, GP, Marburg virus specific minigenome, T7 polymerase, and VP30-GFP. The cells were treated at 15 h p.t. with 0.15 μM nocodazole for 3 h. Time-lapse images of cells were recorded for 90 s; images were captured every 2 s.
Additional file 4 : Movie S4. Huh-7 cells were transfected with plasmids encoding NP, L, VP35, VP24, VP40, GP, Marburg virus specific minigenome, T7 polymerase, and VP30-GFP. The cells were treated at 15 h p.t. with 0.3 μM cytochalasin D for 3 h. Time-lapse images of cells were recorded for 90 s; images were captured every 2 s.
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Cite this article
Takamatsu, Y., Dolnik, O., Noda, T. et al. A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures. Virol J 16, 159 (2019). https://doi.org/10.1186/s12985-019-1267-9
- Marburg virus
- Nucleocapsid-like structures
- Live-cell imaging
- Actin polymerization