Fig. 2

Live-cell imaging system of MARV nucleocapsid-like structures. a The experimental setting for detection of NCLS transport. Huh-7 cells were transfected with plasmids encoding NP, L, VP35, VP24, VP40, GP, Marburg virus specific minigenome, T7 polymerase, and VP30-GFP. b Plasmid-transfected Huh-7 cells were observed at 18 h p.t. The image shows the maximum-intensity projection of time-lapse images of cells, recorded for 90 s; images were captured every 2 s. c, d Over 1000 selected signals were analyzed using the Fiji plugin “TrackMate”. c The length of the NCLS trajectories was evaluated. The y-axis represents the number of signals in each range (x-axis). The numbers indicate mean ± SD (μm). d The velocity of NCLSs transport was evaluated. The y-axis represents the number of signals in each range (x-axis). The numbers indicate mean ± SD (nm/s)