Incidence of human adenoviruses and Hepatitis A virus in the final effluent of selected wastewater treatment plants in Eastern Cape Province, South Africa
© Osuolale and Okoh. 2015
Received: 21 January 2015
Accepted: 16 June 2015
Published: 24 June 2015
Municipal effluent constitutes a large reservoir of human enteric viruses and bacteria. Contemporary monitoring practices rely on indicator bacteria, and do not test for viruses. Different viruses, including Norwalk-like viruses, Hepatitis A virus (HAV), adenoviruses, and rotaviruses, are important agents of illnesses in humans. The burden of disease caused by adenoviruses manifests as pneumonia, bronchiolitis, otitis media, conjunctivitis, and tonsillitis, whereas HAV infection can manifest as acute inflammatory diseases of the liver, fever, anorexia, malaise, nausea, and abdominal discomfort, followed by jaundice and dark urine. The public health implications of these viruses depend upon the physiological status of the wastewater microbial community.
The occurrence of human adenovirus (HAdV) and HAV was determined in the final effluents of five wastewater treatment plants (WWTPs) in the Eastern Cape, South Africa, over 12 months (September 2012–August 2013). The viruses were detected with real-time PCR, and conventional PCR was used for serotyping.
Adenovirus was detected in effluent samples from all five WWTPs and in 64 % of the total samples, whereas HAV was not detected in any effluent sample. At WWPT-A, samples were collected from the final effluent tank (adenoviral concentrations ranged from 1.05 × 101 to 1.10 × 104 genome/L, with a 41.7 % detection rate) and the discharge point (adenoviral concentrations ranged between 1.2 × 101 and 2.8 × 104 genome/L, with a 54.5 % detection rate). At WWPT-B, HAdV was detected in 91.7 % of samples, with viral concentrations of 7.92 × 101–2.37 × 105 genome/L. The HAdV concentrations at WWPT-C were 5.32 × 101–2.20 × 105 genome/L, and the detection rate was 75 %. The adenoviral concentrations at WWPT-D were 1.23 × 103–1.05 × 104 genome/L, and the detection rate was 66.7 %. At WWPT-E, the viral concentrations were 1.08 × 101–5.16 × 104 genome/L, and the detection rate was 54.5 %. Characterization of the adenoviruses revealed HAdV serotypes 2 (1.4 %) and 41 (7.1 %), in species C and F, respectively.
This study is the first to report the prevalence of HAdV in the final effluents of WWTPs in the Eastern Cape, South Africa. The adenoviral detection rates indicate the potential contamination of the environment, with adverse effects on public health.
KeywordsAdenovirus Hepatitis A virus Wastewater Eastern Cape Effluent Public health
Human adenoviruses are ubiquitous in the environment and humans are the only reservoir for them. They are excreted in large numbers in human feces. Although adenoviruses have been reported to infect a variety of animals, they are more reported in humans to be highly specific to them. The viruses persist wherever the environment has been polluted by human feces or sewage [1–3]. Therefore, in natural aquatic environments, the incidence of human adenovirus is probably attributable to contamination with untreated or inefficiently treated sewage . Various variants of adenovirus have been identified, and over 50 serotypes are known  throughout the world [5, 6]. Human adenovirus (HAdV) has been implicated in infections causing gastroenteritis, conjunctivitis, and respiratory diseases , and chronic systemic infections in immunosuppressed individuals [8, 9].
Hepatitis A virus (HAV) is the principal cause of acute hepatitis and is currently recognized as one of the most important human food-borne pathogens in the world. It is responsible for around half the cases of hepatitis diagnosed worldwide. HAV has a worldwide distribution and its presence varies between regions and localities . It can be transmitted via the fecal–oral route, either by person-to-person contact or by the ingestion of contaminated water and food, especially in endemic areas . Like HAdV, humans are the only known reservoir for HAV .
Municipal effluent constitutes a large reservoir of human enteric viruses and bacteria . Contemporary monitoring practices are based on indicator bacteria, and do not test for viruses. Various viruses, including the Norwalk-like viruses, Hepatitis A virus, adenoviruses, and rotaviruses, are important agents of illnesses in humans . Their public health implications depend upon the physiological status of the microbial communities in wastewater . The occurrence of HAdV and HAV in raw water sources reflects the epidemiological features of the environment, including disease outbreaks in particular communities . Globally, adenoviruses have been detected in various types of water, including swimming pools, oceans, river water, and wastewater , and they contaminate the surface waters when pollution enters a water body [14, 15]. HAV and HAdV have been detected in raw and treated water, dams, rivers, and rivers receiving effluent discharges in South Africa [16–20]. Screening stool samples in surveillance programs has confirmed the presence of adenoviral [21, 22] and hepatitis A virus antigens in the samples [23, 24]. The viruses have been detected in the stools of patients in South Africa [19, 25], and gastroenteritis in infants, toddlers, and children has been attributed to them [26, 27].
The epidemiological importance of viruses as water-borne pathogens continues to receive attention, and wastewater is a significant object of research because the diversity of viruses excreted in human waste is high . Human feces and urine can contain enormous amounts of enteric viruses excreted from infected individuals. Therefore, wastewater is one of the major concentrated sources of these viruses . The detection of water-borne viruses is very important to public health, in the deterrence of illness and in response to outbreaks. Although these viruses are considered important, insufficient data are available to evaluate their prevalence and distributions in the environment . Although their presence in the environment is of considerable concern to public health, no health guidelines or regulations exist to provide a baseline for monitoring these viruses in the environment .
PCR methods allow the simultaneous detection of multiple viruses. It can also be used to monitor specific viruses, which may complement the bacterial indicators already used. Some enteric viruses, like the enterovirus, adenovirus, and orthoreovirus groups, are most readily detected with the currently available methods and are recommended for routine monitoring . Another benefit of quantitative real-time PCR is that it permits the evaluation of adenoviral concentrations in environmental samples . In this study, we assessed the prevalence of HAdV and HAV in effluent samples collected from five wastewater treatment plants (WWTPs) located in the Eastern Cape, South Africa. The WWTPs were selected based on their proximity to communities (rural, suburban, and urban areas), treatment technologies, and the lack of research data available for them. To the best of our knowledge, this is the first study of viruses present in the final effluents of WWTPs in the Eastern Cape Province of South Africa.
PCR specificity, sensitivity, standard curves, and detection limits
HAdV and HAV were examined with real-time PCR assays. The reactivity of the primers and probes were observed using viral DNA and RNA standards as the templates. Real-time PCR detected HAdV 41 and HAV. The resulting standard curves (HAdV 41, slope −3.53 and Y-intercept 28.34; HAV, slope −3.22 and Y-intercept 36.64) had strong correlation coefficients (r2) of 0.99 and 0.98, respectively. The PCR amplification efficiency for the reactions exceeded 92 %. No product was amplified in the negative controls, confirming the absence of PCR carryover contamination.
Quantification of human enteric viruses in wastewater
Adenoviral species and serotypes
Typing the HAdV detected in the effluent samples indicated the presence of two of the four assayed species of adenovirus. The samples analyzed were positive for adenoviral species C and F. Adenoviral species C was positive for serotype 2 and negative for serotypes 1, 5, and 6. Adenoviral species F was positive for serotype 41, whereas serotype 40 was not detected. Five Adenoviral serotype 41 of the species F was detected in five samples (7.1 %), and was the most prevalent serotype, followed by serotype 2 of adenovirus species C (1.4 %). No other species or serotype was detected.
HAV was not detected in any of the samples collected from four WWTPs. However, it was detected at WWTP-D in 41.7 % of the samples analyzed. However, the viral concentration was ≤ 1 genome copy/L, below the set detection limit.
In this study, we provide conclusive evidence of the presence of HAdV in effluent samples from the East Cape Province, together with its genomic concentrations, but HAV was generally not detected. The total genomic copies of HAdV detected reflect the concentrations of the virus being released into the environment. In this study, HAdV was detected in 64 % of samples and HAV was detected in none. These findings are similar to those of previous studies, which reported HAdV in ≥ 50 % of wastewater and environmental water samples [29, 33–35]. The failure to detect HAV in this study is consistent with previous reports [35, 36], and other studies have shown that HAV is the least detected of the enteric viruses . High concentrations of HAV have been found in sewage samples, with higher concentrations in raw samples than in treated samples . It is possible that HAV was present during the course of the 12-month sampling period at concentrations below our detection limit (10 genome/L). However, HAV was only detected at WWTP-D and at very low concentrations, far below the set detection limit.
High detection rates and viral concentrations of HAdV occurred in winter, between June and August 2013, at all the WWTPs tested. During winter, the treatment efficiency of the WWTPs is reduced because low temperatures negatively affect the process units, which in turn affects the quality of the treated effluent [39, 40]. The detection of HAdV specifically in winter has been reported by Rigotto et al. , Haramoto et al. , and Haramoto et al. . Furthermore, infections attributable to adenovirus are reported by Modarres and Jam-Afzon  to peak in winter, while remaining low in summer .
In the present study, we detected no virus at high chlorine concentrations of > 0.20 mg/L (data not shown), whereas at chlorine concentrations ≥ 0.15 mg/L, low concentrations of HAdV were detected, specifically in the WWTP-A and WWTP-E treated effluents. The the other WWTPs showed high detection levels, where effluent quality was poor and chlorine disinfection inefficient. This was evident at WWPT-B and WWPT-C, and WWPT-B recorded the highest concentrations and detection rates of HAdV. Simmons and Xagoraraki  and the Water Research Foundation  reported that, given the right treatment system and the correct configuration of treatment process, chlorine disinfection will inactivate any HAdV in the effluent. Studies by Thurston-Enriquez et al. [47, 48] demonstrated the inactivation of HAdV 40 with chlorine and chlorine dioxide. They found that the disinfection process is effective at pH 5–8 and temperatures of 5–15 °C, and that contact times < 30 min are sufficient to inactivate HAdV 40. However, they were quick to point out the shielding effects associated with particular matter, which could explain the inefficient disinfection at the WWTPs with high viral loads.
Most of the HAdV serotypes in the effluents were from species C and F. The detection rates for species C and F were 2.9 % and 14.3 %, respectively. A similar study by Van Heerden et al.  identified serotypes 2 and 41 in river water receiving wastewater discharge. The incidence of these two serotypes in wastewater effluents has also been reported by Fong et al.  and Kuo et al. , who detected HAdV 41 more frequently than HAdV 2, which is consistent with our findings. Sibanda and Okoh  reported the detection of species C and F HAdV in river water in the Eastern Cape. A study of HAdV in sewage from the Taiwan area reported the presence of both adenoviral species C and F, with a preponderance of HAdV serotype 41 . However, in contrast to the report of Sibanda and Okoh , more species C HAdV than species F was detected in river water receiving wastewater discharge in the present study. Respiratory illnesses have been attributed to species C adenoviruses , whereas species F is considered one of the major causes of viral gastroenteritis . Species F is reported to cause serious infections in immunocompromised individuals , and is often a coinfection in patients with Human immunodeficiency virus infections .
The high detection rate and high concentrations of HAdV in the final effluents demonstrate the prevalence of the virus in the environment and the disease burden this virus poses in the community. Throughout the months of sampling, the virus was detected regularly at some WWTPs. Wastewater treatment, access to sanitation, and the restoration and rehabilitation of the existing wastewater infrastructure are under discussion at present. With greater droughts occurring globally and the need to move from wastewater treatment to resource recovery, and particularly water recycling, it is extremely important to understand the concentrations of viral pathogens. Real-time PCR, with its high specificity for adenoviruses, is a reliable tool for monitoring viral contamination and pollution in the environment. The use of HAdV as an indicator of fecal contamination is recommended because it is reportedly more stable in the environment and more abundant than other enteric viruses [6, 51]. The frequency of detection of this HAdV in this study supports these views.
In this study, a 12-month sampling program was conducted at five WWTPs to determine the prevalence of HAdV and HAV in their final effluents. The identification and confirmation of HAdV serotype 2 (species C) and HAdV serotype 41 (species F) as the predominant adenoviral species in this study does not necessarily imply the absence of other serotypes. However, their presence signifies the imminent danger posed to public health by the discharge of poorly treated effluent into the environment because these two adenoviral species have been implicated in clinical illnesses. The presence of viral genomes indicates that the quality of the effluent is low from the perspective of infection risk, although other infectivity assays should be performed to corroborate the potential infectivity of these viruses. To our knowledge, this is the first study to demonstrate the presence and prevalence of HAdV and HAV in the final effluents of WWTPs in the Eastern Cape Province of South Africa.
Materials and methods
For confidentiality, the WWTPs are listed as WWTP-A, WWTP-B, WWTP-C, WWTP-D, and WWTP-E. WWTP-A operates an activated sludge system with a design capacity of about 8 ML/day; WWTP-B has a design capacity of 5 ML/Day and operates a biofilter/PETRO® (pond enhanced treatment and operation) treatment system; WWTP-C operates an activated sludge system with a design capacity of 40 ML/day; WWTP-D operates both a biofilter and an activated sludge system, with a design capacity of 12 ML/day; and WWTP-E has a design capacity of 1.8 ML/day and operates a biofilter system. All the WWTPs use chlorine disinfection.
Samples were collected from the five WWTPs from September 2012 to August 2013, at two sampling points for WWTP-A: the final effluent point (FE) just after chlorination and the discharge point (DP), immediately before the wastewater is discharged into the river. WWTP-B, WWTP-C, WWTP-D, and WWTP-E were sampled at FE only, their DP was inaccessible. Effluent samples were collected in sterile 1.7-L Nalgene bottles containing sodium thiosulfate to dechlorinate the samples. A cooler box was used to store all the samples and transport them to the laboratory for processing within 2 h. The effluent samples were collected as part of the routine surveillance of enteric viruses at each WWTP. The samples were collected once a month at each WWTP (n = 12). No samples were collected at WWTP-A (DP) in December 2012 or at WWPT-E in September 2012 because climatic conditions were unfavorable, so a total of 70 samples were processed.
Concentration of water samples for viral detection
The viruses in the effluent samples were concentrated with the adsorption–elution method, as described by Haramoto et al. , with some modifications. A sample (5 mL) of 250 mM AlCl3 was passed through a Millipore type HA filter held for 5 min (0.45-μm pore size and 47-mm diameter) to generate a cation (Al3+)-coated filter, which was attached to a 250-mL Millipore sterile filtration system on t3-place filtration manifold. A 1.25-L sample of effluent was passed through the filter, and 200 mL of 0.5 mM H2SO4 was then filtered through the membrane. The viral particles were eluted into a Petri dish with 10 mL of 1 mM NaOH. The eluates were placed in Centriprep™ Centrifugal Filter Units with Ultracel-50 membranes, containing 0.1 mL of 50 mM H2SO4 and 0.1 mL of 100 × Tris–EDTA (TE) buffer to neutralize them before further concentration. The Centriprep™ YM-50 ultra-filtration device (Millipore) was centrifuged to produce a final volume of approximately 700 μL. In exceptional cases, when the eluate was turbid, the centrifugation time was increased and the clogged membrane was cleared with sterile forceps. The concentrated samples were stored at −80 °C until use.
The prototype strains of HAdV (ATCC VR-931, strain Dugan) and HAV (ATCC VR-1357, strain PA21) used in this work were obtained from the American Type Culture Collection (ATCC, Rockville, MD).
Extraction of viral nucleic acids
Viral nucleic acids were extracted from 200 μL of the concentrated effluent samples with Quick-gDNA™ MiniPrep and a Zymo Viral RNA Extraction Kit (Zymo Research Corporation, 17062 Murphy Ave. Irvine, CA 92614, U.S.A) using the spin column technique, according to the manufacturer’s instructions. All samples were tested for the presence of HAdV and HAV nucleic acids with real-time PCR.
Quantification of viral genomes with real-time PCR
Primers and probes for real-time RT–PCR and qPCR
Primers and Labelled TaqMan Probe
Hepatitis A virus
HAV68 (F): 5′-TCA CCG CCG TTT GCC TAG-3′
HAV240 (R): 5′-GGA GAG CCC TGG AAG AAA G-3′
HAV150 (P): 5′-FAM-CCT GAA CCT GCA GGA ATT AA- MGBNFQ-3′
The HAV RNA virus was quantified with a two-step protocol, in which the RNA was first transcribed into cDNA in a separate reverse-transcription step. Briefly, 10 μL of template RNA, 1 μL of 100 μM random hexamer primer, 1 μL of 100 mM dNTP mix, 2.5 μL diethylpyrocarbonate (DEPC)-treated water, 4 μL of 5 × RT buffer, 0.5 μL of 40 U/μL RiboLock RNase Inhibitor, and 1 μL of 200 U/μL RevertAid Premium Reverse Transcriptase (Fermentas Life Sciences) were added in the order indicated to a 0.5-mL PCR tube on ice, mixed by vortexing briefly, and centrifuged (15,000 × g). The tube was incubated at 25 °C for 10 min, and then for 30 min at 60 °C. The reaction was terminated by heating at 85 °C for 5 min. An aliquot of 5 μL of the resultant cDNA was used as the template for a real-time qPCR reaction containing reagents in the same proportions as were used to amplify HAdV. HAV strain PA21 (ATCC VR-1357) was used as the positive control. Fluorescence data were collected at the end of the annealing step.
Identification of adenoviral species and serotypes
Primers for the detection of adenoviral serotypes
Sequence (5′ to 3′)
CAC TTA ATG CTG ACA CG
ACT GGA TAG AGC TAG CG
Prevention of PCR carryover contamination
All standard precautions were taken to prevent PCR contamination, with adherence to strict laboratory practices. The pre-PCR manipulations (DNA isolation and PCR set-up) were performed in a clean room that was physically isolated from the real-time PCR machine and the post-PCR processing area. Dedicated pipettes and reagents were used at each location. Negative controls were run with all assays, and no indications of contamination were detected. The DNA used to generate the standard curves was prepared in a separate room.
Sensitivity and specificity studies
To validate the real-time PCR assays before their application to the effluent samples, the detection limit and amplification efficiency of each reaction were determined as described by Simmons and Xagoraraki . The sensitivity of our real-time PCR assay was evaluated with the nucleic acid from a stock culture of HAV and with HAdV DNA from a serial seven-fold dilution of a genomic extract. A detection limit of 10 copies of target DNA per reaction was set for all PCR assays.
Standard curve construction
Standard curves were generated to quantify the sensitivity of the assays using stocks of HAdV and HAV. The extracted DNA and RNA were prepared and their concentrations determined spectrophotometrically with a Qubit® 1.0 Fluorometer (Life Technologies), according to the manufacturer’s instructions. The viral DNA and RNA were serially diluted seven-fold in nuclease-free water to generate the standard curves. All the standard curve reactions were run in triplicate.
The authors would like to thank the Water Research Commission of South Africa and the South African Medical Research Council for their financial support.
- Pond K. Water Recreation and Disease: Plausibility of Associated Infections- Acute Effects, Sequelae and Mortality. 2005.Google Scholar
- Ministry of Health: Guidelines for Drinking-Water Quality Management for New Zealand. Wellington; 2013.Google Scholar
- Jiang SC. Human adenoviruses in water: occurrence and health implications: a critical review. Environ Sci Technol. 2006;40:7132–40.PubMedView ArticleGoogle Scholar
- Blyn LB, Hall T a, Libby B, Ranken R, Sampath R, Rudnick K, et al. Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry. J Clin Microbiol. 2008;46:644–51.PubMed CentralPubMedView ArticleGoogle Scholar
- Schmitz H, Wigand R, Heinrich W. Worldwide epidemiology of human adenovirus infections. Am J Epidemiol. 1983;117:455–66.PubMedGoogle Scholar
- Mena KD, Gerba CP. Waterborne adenovirus. Rev Environ Contam Toxicol. 2009;198:133–67.PubMedGoogle Scholar
- Kuo DH-W, Simmons FJ, Blair S, Hart E, Rose JB, Xagoraraki I. Assessment of human adenovirus removal in a full-scale membrane bioreactor treating municipal wastewater. Water Res. 2010;44:1520–30.PubMedView ArticleGoogle Scholar
- Selvaraj G, Kirkwood C, Bines J, Buttery J. Molecular epidemiology of adenovirus isolates from patients diagnosed with intussusception in Melbourne, Australia. J Clin Microbiol. 2006;44:3371–3.PubMed CentralPubMedView ArticleGoogle Scholar
- Jong J De. Epidemiology of enteric adenoviruses 40 and 41 and other adenoviruses in immunocompetent and immunodeficient individuals. Perspect Med Virol 2003:407–445.Google Scholar
- Kokkinos P, Ziros P, Filippidou S, Mpampounakis I, Vantarakis A. Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece. Virol J. 2010;7:235.PubMed CentralPubMedView ArticleGoogle Scholar
- De Paula VS, Baptista ML, Lampe E, Niel C, Gaspar AMC. Characterization of hepatitis A virus isolates from subgenotypes IA and IB in Rio de Janeiro, Brazil*. J Med Virol. 2002;27(May 2001):22–7.View ArticleGoogle Scholar
- Morace G, Aulicino F a, Angelozzi C, Costanzo L, Donadio F, Rapicetta M. Microbial quality of wastewater: detection of hepatitis A virus by reverse transcriptase-polymerase chain reaction. J Appl Microbiol. 2002;92:828–36.PubMedView ArticleGoogle Scholar
- Perdek JM, Arnone RD, Stinson MK, Tuccillo ME: Managing Urban Watershed Pathogen Contamination. Cincinnati, Ohio; 2003.Google Scholar
- Hundesa A, Maluquer de Motes C, Bofill-Mas S, Albinana-Gimenez N, Girones R. Identification of human and animal adenoviruses and polyomaviruses for determination of sources of fecal contamination in the environment. Appl Environ Microbiol. 2006;72:7886–93.PubMed CentralPubMedView ArticleGoogle Scholar
- Jiang S, Noble R, Chu W. Human adenoviruses and coliphages in urban runoff-impacted coastal waters of Southern California. Appl Environ Microbiol. 2001;67:179–84.PubMed CentralPubMedView ArticleGoogle Scholar
- Van Heerden J, Ehlers MM, Heim A, Grabow WOK. Prevalence, quantification and typing of adenoviruses detected in river and treated drinking water in South Africa. J Appl Microbiol. 2005;99:234–42.PubMedView ArticleGoogle Scholar
- Sibanda T, Okoh AI. Assessment of the incidence of enteric adenovirus species and serotypes in surface waters in the eastern cape province of South Africa: Tyume River as a case study. ScientificWorldJournal. 2012;2012:949216.PubMed CentralPubMedView ArticleGoogle Scholar
- Genthe B, Gericke M, Bateman B, Mjoli N, Kfir R. Detection of enteric adenoviruses in south african waters using gene probes. Water Sci Technol. 1995;31:345–50.View ArticleGoogle Scholar
- Taylor MB, Cox N, Vrey MA, Grabow WOK. The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa. Water Res. 2001;35:2653–60.PubMedView ArticleGoogle Scholar
- Chigor VN, Okoh AI. Quantitative RT-PCR detection of hepatitis A virus, rotaviruses and enteroviruses in the Buffalo River and source water dams in the Eastern Cape Province of South Africa. Int J Environ Res Public Health. 2012;9:4017–32.PubMed CentralPubMedView ArticleGoogle Scholar
- Audu R, Omilabu AS, Peenze I, Steele DA. Isolation and identification of adenovirus recovered from the stool of children with diarrhoea in Lagos, Nigeria. Afr J Health Sci. 2004;9.Google Scholar
- Pring-Åkerblom P, Adrian T, Köstler T. PCR-based detection and typing of human adenoviruses in clinical samples. Res Virol. 1997;148:225–31.PubMedView ArticleGoogle Scholar
- Polish LB, Robertson BH, Khanna B, Krawczynski K, Spelbring J, Olson F, et al. Excretion of hepatitis A virus (HAV) in adults: Comparison of immunologic and molecular detection methods and relationship between HAV positivity and infectivity in tamarins. J Clin Microbiol. 1999;37:3615–7.PubMed CentralPubMedGoogle Scholar
- De Filippis P, Divizia M, Mele A, Adamo B, Paná A. Detection of Hepatitis A virus in the stools of healthy people from endemic areas. Eur J Epidemiol. 1987;3:172–5.PubMedView ArticleGoogle Scholar
- Moore PL, Steele AD, Alexander JJ. Relevance of commercial diagnostic tests to detection of enteric adenovirus infections in South Africa. J Clin Microbiol. 2000;38:1661–3.PubMed CentralPubMedGoogle Scholar
- Taylor MB, Marx FE, Grabow WO. Rotavirus, astrovirus and adenovirus associated with an outbreak of gastroenteritis in a South African child care centre. Epidemiol Infect. 1997;119:227–30.PubMed CentralPubMedView ArticleGoogle Scholar
- Phan TG, Nguyen T a, Yan H, Okitsu S, Ushijima H. A novel RT-multiplex PCR for enteroviruses, hepatitis A and E viruses and influenza A virus among infants and children with diarrhea in Vietnam. Arch Virol. 2005;150:1175–85.PubMedView ArticleGoogle Scholar
- Cantalupo PG, Calgua B, Zhao G, Hundesa A, Wier AD, Katz JP, et al. Raw sewage harbors diverse viral populations. MBio. 2011;2:e00180–11.PubMed CentralPubMedView ArticleGoogle Scholar
- Simmons FJ, Xagoraraki I. Release of infectious human enteric viruses by full-scale wastewater utilities. Water Res. 2011;45:3590–8.PubMedView ArticleGoogle Scholar
- Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD. Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 2005;71:3131–6.PubMed CentralPubMedView ArticleGoogle Scholar
- Singh A. Surveillance of Microbial Pathogens in the Umgeni River. Durban South Africa: University of KwaZulu-Natal; 2013.Google Scholar
- Hartmann NM, Dartscht M, Szewzyk R, Selinka H-C. Monitoring of adenovirus serotypes in environmental samples by combined PCR and melting point analyses. Virol J. 2013;10:190.PubMed CentralPubMedView ArticleGoogle Scholar
- Fong T-T, Phanikumar MS, Xagoraraki I, Rose JB. Quantitative detection of human adenoviruses in wastewater and combined sewer overflows influencing a Michigan river. Appl Environ Microbiol. 2010;76:715–23.PubMed CentralPubMedView ArticleGoogle Scholar
- Spilki FR, Luz RB d, Fabres RB, Soliman MC, Kluge M, Fleck JD, et al. Detection of human adenovirus, rotavirus and enterovirus in water samples collected on dairy farms from Tenente Portela, Northwest of Rio Grande do Sul, Brazil. Brazilian J Microbiol. 2013;44:953–7.View ArticleGoogle Scholar
- Vantarakis A, Papapetropoulou M. Detection of enteroviruses, adenoviruses and hepatitis a viruses in Raw sewage and treated effluents by nested-PCR. Water Air Soil Pollut. 1999;114:85–93.View ArticleGoogle Scholar
- Prado T, Fumian TM, Miagostovich MP, Gaspar AMC. Monitoring the hepatitis A virus in urban wastewater from Rio de Janeiro, Brazil. Trans R Soc Trop Med Hyg. 2012;106:104–9.PubMedView ArticleGoogle Scholar
- Hellmér M, Paxéus N, Magnius L, Enache L, Arnholm B, Johansson A, et al. Detection of pathogenic viruses in sewage provided early warnings of hepatitis A virus and norovirus outbreaks. Appl Environ Microbiol. 2014;80:6771–81.PubMed CentralPubMedView ArticleGoogle Scholar
- Villar LM, de Paula VS, Diniz-Mendes L, Guimarães FR, Ferreira FFM, Shubo TC, et al. Molecular detection of hepatitis A virus in urban sewage in Rio de Janeiro, Brazil. Lett Appl Microbiol. 2007;45:168–73.PubMedView ArticleGoogle Scholar
- Gagnon V, Maltais-Landry G, Puigagut J, Chazarenc F, Brisson J. Treatment of hydroponics wastewater using constructed wetlands in winter conditions. Water Air Soil Pollut. 2010;212:483–90.View ArticleGoogle Scholar
- Massé DI, Masse L. Characterization of wastewater from hog slaughterhouses in Eastern Canada and evaluation of their in-plant wastewater treatment systems. Can Biosyst Eng/Le Genie des Biosyst au Canada. 2000;42:139–46.Google Scholar
- Rigotto C, Victoria M, Moresco V, Kolesnikovas CK, Corrêa AA, Souza DSM, et al. Assessment of adenovirus, hepatitis A virus and rotavirus presence in environmental samples in Florianopolis, South Brazil. J Appl Microbiol. 2010;109:1979–87.PubMedView ArticleGoogle Scholar
- Haramoto E, Katayama H, Oguma K, Ohgaki S. Application of cation-coated filter method to detection of noroviruses, enteroviruses, adenoviruses, and torque teno viruses in the Tamagawa River in Japan. Appl Environ Microbiol. 2005;71:2403–11.PubMed CentralPubMedView ArticleGoogle Scholar
- Haramoto E, Katayama H, Oguma K, Ohgaki S. Quantitative analysis of human enteric adenoviruses in aquatic environments. J Appl Microbiol. 2007;103:2153–9.PubMedView ArticleGoogle Scholar
- Modarres S, Jam-Afzon F. Enteric adenovirus infection in infants and young children with acute gastroenteritis in Tehran. Acta Med Iran. 2006;44:349–53.Google Scholar
- Hamkar R, Yahyapour Y, Noroozi M, Nourijelyani K, Jalilvand S, Adibi L, et al. Prevalence of rotavirus, adenovirus, and astrovirus infections among patients with acute gastroenteritis in, Northern Iran. Iran J Public Health. 2010;39:45–51.PubMed CentralPubMedGoogle Scholar
- WRF:Water Research Foundation: Challenge Organisms for Inactivation of Viruses by Ultraviolet Treatment. 2010.Google Scholar
- Thurston-Enriquez JA, Haas CN, Jacangelo J, Gerba CP. Chlorine Inactivation of Adenovirus Type 40 and Feline Calicivirus. Appl Environ Microbiol. 2003;69:3979–85.PubMed CentralPubMedView ArticleGoogle Scholar
- Thurston-Enriquez JA, Haas CN, Jacangelo J, Gerba CP. Inactivation of enteric adenovirus and feline calicivirus by chlorine dioxide. Appl Environ Microbiol. 2005;71:3100–5.PubMed CentralPubMedView ArticleGoogle Scholar
- Shih 施孟欣 T A - Meng-Hsin: 台灣地區水體環境中腺病毒之親源分析 TT - Phylogenetic Analyses of Human Adenoviruses (HAdVs) In Sewages of Taiwan Area. Volume 碩士. 東海大學; 2013.Google Scholar
- Kolawole O, Oladosu T, Abdulkarim A, Okoh A: Adenovirus respiratory tract and HIV co-infections in patients attending the University Of Ilorin, Teaching Hospital, Ilorin. sci-int.com 2013, 25:501–508.Google Scholar
- Katayama H, Haramoto E, Oguma K, Yamashita H, Tajima A, Nakajima H, et al. One-year monthly quantitative survey of noroviruses, enteroviruses, and adenoviruses in wastewater collected from six plants in Japan. Water Res. 2008;42:1441–8.PubMedView ArticleGoogle Scholar
- Haramoto E, Katayama H, Utagawa E, Ohgaki S. Recovery of human norovirus from water by virus concentration methods. J Virol Methods. 2009;160:206–9.PubMedView ArticleGoogle Scholar
- Costafreda MI, Bosch A, Pintó RM. Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay for quantification of hepatitis A virus in clinical and shellfish samples. Appl Environ Microbiol. 2006;72:3846–55.PubMed CentralPubMedView ArticleGoogle Scholar
- Pintó RM, Costafreda MI, Bosch A. Risk assessment in shellfish-borne outbreaks of hepatitis A. Appl Environ Microbiol. 2009;75:7350–5.PubMed CentralPubMedView ArticleGoogle Scholar
- Metzgar D, Osuna M, Yingst S. PCR analysis of Egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections. J Clin Microbiol. 2005;43:5743–52.PubMed CentralPubMedView ArticleGoogle Scholar
- Tiemessen C, Nel M: Detection and typing of subgroup F adenoviruses using the polymerase chain reaction. J Virol Methods 1996.Google Scholar
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