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Table 1 Primers and probes for real-time RT–PCR and qPCR

From: Incidence of human adenoviruses and Hepatitis A virus in the final effluent of selected wastewater treatment plants in Eastern Cape Province, South Africa

Enteric virus

Primers and Labelled TaqMan Probe

Reference

Hepatitis A virus

HAV68 (F): 5′-TCA CCG CCG TTT GCC TAG-3′

[53, 54]

HAV240 (R): 5′-GGA GAG CCC TGG AAG AAA G-3′

HAV150 (P): 5′-FAM-CCT GAA CCT GCA GGA ATT AA- MGBNFQ-3′

Adenovirus

JTVX(F) 5′-GGACGCCTCGGAGTACCTGAG-3′

[30]

JTVX(R) 5′-ACIGTGGGGTTTCTGAACTTGTT-3′

JTVX(P) 5′-FAM-CTGGTGCAGTTCGCCCGTGCCA-MGBFQ-3′

  1. F forward/sense, R reverse/antisense, P probe, FAM 6-carboxyfluorescein (reporter dye), MGBNFQ minor groove binder/nonfluorescent quencher