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Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay



There is widespread interest in the use of innate immune modulators as a defense strategy against infectious pathogens. Using rotavirus as a model system, we developed a cell-based, moderate-throughput screening (MTS) assay to identify compounds that reduce rotavirus infectivity in vitro, toward a long-term goal of discovering immunomodulatory agents that enhance innate responses to viral infection.


A natural product library consisting of 280 compounds was screened in the assay and 15 compounds that significantly reduced infectivity without cytotoxicity were identified. Time course analysis of four compounds with previously characterized effects on inflammatory gene expression inhibited replication with pre-treatment times as minimal as 2 hours. Two of these four compounds, α-mangostin and 18-β-glycyrrhetinic acid, activated NFκB and induced IL-8 secretion. The assay is adaptable to other virus systems, and amenable to full automation and adaptation to a high-throughput format.


Identification of several compounds with known effects on inflammatory and antiviral gene expression that confer resistance to rotavirus infection in vitro suggests the assay is an appropriate platform for discovery of compounds with potential to amplify innate antiviral responses.


Interest in the use of innate immune modulating agents recently has increased in the context of developing effective biodefense strategies. Increasing natural disease resistance by administration of agonists that stimulate pathogen recognition receptors and gene expression pathways is an approach that would provide broad protection from infection without need for pathogen-specific vaccines. Stimulating broadly reactive immune responses to viral, as well as bacterial and protozoan pathogens, has shown efficacy in animal models and is the subject of recent reviews that address the utility of immune potentiators in developing infectious disease defense strategies [1, 2]. The number of such compounds currently in clinical development lends support for this approach [3].

To be seriously considered as a candidate antiviral drug or innate immune agonist, rapid and quantitative assessment of activity and toxicity in cell culture are prerequisite to lead compound development [4]. Therefore, development of screening assays in cell lines that can simultaneously support virus replication and be responsive to measures of inhibition of virus replication are a priority. Cell-based high-throughput screening (HTS) assays that test for compounds active against hepatitis C virus (HCV), HIV and SARS coronavirus utilize recombinant viruses and changes in reporter gene expression in engineered cell lines to measure antiviral activity [58]. For example, a dual replicon assay system that combined reporter gene assays and FRET was used to screen for drugs effective against HCV [5]. Advantages of these cited assays include rapid readout and an ability to perform screens with viruses that must be handled under elevated biosafety conditions.

The intent of the studies reported here was to establish a cell-based screening assay that could identify compounds that inhibit virus replication by inducing antiviral gene expression pathways. Although the assay could identify compounds that target distinct steps of the virus replication cycle, we are interested in those that stimulate cellular responses necessary to confer initial resistance to rotavirus infection. The theory behind this approach is that amplification of the antiviral response will override virus-encoded immune evasion strategies and restrict replication to subclinical levels.

Rotaviruses are responsible for the majority of childhood morbidity and mortality from viral gastroenteritis [9]. Several rotavirus vaccines in clinical trials show promising efficacy, suggesting that a long-term goal of rotavirus gastroenteritis becoming a vaccine preventable disease is attainable [10]. However, the significant mortality associated with rotavirus illness in the developing world suggests approaches to enhance the antiviral immune response and consequent natural resistance to rotavirus infection need to be explored. The importance of this issue and the significant amount of data available on rotavirus replication led us to use this virus as a model system. We report development of an assay that uses unmodified adherent epithelial cells to measure reductions in rotavirus infectivity in response to treatment of cells with a variety of compounds. A natural product library consisting of 280 compounds derived from plant extracts was screened, and several compounds that inhibited rotavirus infectivity in a dose-dependent manner were identified. All of the compounds that passed the designated criteria of a hit have been reported to have direct effects on inflammatory or antiviral gene expression or on virus replication. Development of such a platform to screen compounds for the ability to diminish virus replication easily can be applied to other virus systems where direct measurement of activity in epithelial cells is desirable.


Screening assay development

An ELISA-based assay that successfully measured neutralization of rotavirus infectivity has been reported [11]. Similarly, we adapted an immunofluorescent (IF) infectivity assay to a moderate-throughput screening (MTS) format to measure changes in rotavirus infectivity following treatment of cells with compounds from a natural product library. In the standard IF assay, MA104 cells are cultured to confluence in 96-well microtiter plates, then infected with rotavirus in triplicate wells and incubated for 18–20 hours. Virus replication then is detected by indirect IF and replication is quantified by counting fluorescent focus forming units (FFU) in a dilution series by fluorescence microscopy. To adapt the assay to an MTS format that would necessarily eliminate manual counting of FFU, an HRP-conjugated secondary antibody was used in place of the FITC-conjugated antibody, and the assay was developed with chemiluminescent substrate. Virus replication then was quantified in a microplate fluorometer with readout of relative light units (rlu). Use of an enzymatic signal to measure infectivity was validated by comparing changes in chemiluminescent signal versus virus dose measured by IF. The data shown in figure 1 illustrate the range of increase in chemiluminescent signal that corresponds with increasing infectious units in a 10-1/3 virus dilution series. These data demonstrate that changes in the magnitude of the enzymatic signal accurately reflect changes in the number of infectious units measured by IF.

Figure 1
figure 1

Luminescent signal magnitude corresponds with infectious units. Cells were infected with 10-1/3 serial dilutions of rotavirus for 18 hours. Plates were fixed and probed with anti-VP6 mAb A6M followed by peroxidase-conjugated goat anti-mouse IgG. Signals were developed with chemiluminescent substrate and measured on a ThermoElectron Fluroskan and scored as relative light units (rlu). Error bars are standard errors of the means (n = 5).

In order to establish internal inhibition controls for the assay, we measured reductions in infectivity in response to cytokines known to induce antiviral gene expression. Cells were pre-treated with IFNα, IFNγ, or a combination of both cytokines. IFNα and IFNγ both reduced infectivity, and the magnitude of reduction increased to ~80% when cells were treated with the combination of both cytokines (Figure 2). The combination of IFNα and IFNγ consistently yielded the greatest reduction in virus replication. Therefore, this mixture of cytokines, and IL-2 which is not known to have direct antiviral activity, were included as internal positive and negative controls, respectively, in each plate of every experiment.

Figure 2
figure 2

Establishment of internal controls: IFNα and IFNγ reduce rotavirus infectivity in the assay. Cells were treated with indicated amounts of each cytokine or a combination of both prior to infection. Infections were allowed to proceed for 18 hours and chemiluminescent measurement of reduction in rotavirus infectivity was performed as described in the text and in the legend to Figure 1. Error bars are standard errors of the means.

Assay validation

Plate uniformity assessments were performed according to the recommendations of the NIH Chemical Genomics Center in order to determine whether our assay was suitable for eventual adaptation to a high-throughput format. The Z' coefficient was established with mock infected cells representing the minimum signal value, virus infected cells representing the maximum signal value, and the IFNα/IFNγ mixture as the midrange signal. Experiments consisting of three plates each run on three consecutive days were performed and an example of the data from one day is shown in Figure 3. No evidence of significant drift or edge effect was observed. The Z' coefficient measures the quality of an HTS assay by comparing data variation and the signal dynamic range, and a value of > 0.5 establishes the assay as excellent for screening [12, 13]. The Z' values ranged from 0.69 – 0.82 for individual plates, and the aggregate Z' value from all plates and all days was 0.64. A signal-to-noise value of 4.96 was calculated with the aggregate data from all plates. All of the statistical criteria for intra-plate assessment were met [14].

The assay results show some inter-plate variability. For example, although all within-day fold-shifts in signal were less than 2, all average (between)-day fold-shifts were not. We expect some degree of variability due simply to the variations in the growth status of cells from day to day. Importantly, we established sufficient intra-plate controls to account for minor variances associated with cell status. Together the validation and statistical data support the assay as a viable platform for screening compound libraries.

Figure 3
figure 3

Assay validation. Cells were treated according the provided template [14]. Three individual experiments, each containing three plates, were conducted on three separate days. A) The left panels show a representative set of data from day two of the plate uniformity assay with the data graphed in a well-row orientation where the wells are labeled horizontally. The panels on the right contain the same data in a well-column orientation. B) Aggregate data of all nine plates over three days in a well-row orientation.

Compounds from a natural product library reduce rotavirus infectivity

A 280 compound natural product library was screened to identify compounds that reduced rotavirus infectivity. Forty-seven (17%) compounds were selected for a second round of screening and were tested both for dose-dependency and for effects on cell viability. A representative dataset is shown in Figure 4. Figure 4A shows data obtained from the initial screen, and the data in figure 4B illustrate dose-dependent reductions in rotavirus infectivity in response to treatment of cells with each of the four representative compounds. The observed reduction in infectivity was not a result of generalized cytotoxicity because cell viability did not decrease upon treatment beyond the DMSO only controls (Figure 4B). The one exception was that mangostin was toxic at 10 μg/ml. Dose-dependency and cell viability assays were performed in parallel with cells seeded at the same densities from the same cell suspension.

Figure 4
figure 4

Natural products identified in the MTS assay reduce rotavirus infectivity in a dose-dependent manner. Representative data are shown for four compounds: 18-β-glycyrrhetinic acid, abietic acid, mangostin, and all-trans retinoic acid. A) Data from initial screen. The y-axis is relative light units (n = 3). B) Secondary screen that includes a dose-response (red) and cell viability (blue) data. The left y-axis is relative light units and the right y-axis is viability (n = 3). Error bars are standard errors of the means. The highest concentration of mangostin was toxic under the conditions of this assay.

Forty-seven compounds from the primary screen were subjected to second round screening and 32 were eliminated from consideration for follow-up studies. Ten compounds did not reduce infectivity above the established threshold in the second round, generating a true false positive hit rate of ~20%. Twenty compounds proved cytotoxic as measured by the cell viability assay, and two compounds are known toxins with gross effects on cell metabolism (e.g. protein synthesis inhibitors). The two toxins were eliminated from further consideration because we are interested in identifying compounds that stimulate or enhance antiviral signaling pathways. The remaining 15 compounds were designated true hits (5 %) and are listed in Table 1.

Table 1 Compounds that reduce rotavirus infectivity without cytotoxicity

Time course of inhibitory effects

The four compounds shown as representative data in Figure 4 were analyzed further because of prior reports of their effects on components of innate immune signaling pathways [1517]. A time-course and dose-response of each compound were established in the immunofluorescent focus reduction assay. Cells were treated with each compound for various times ranging from 12 hours pre-infection to 2 hours post-infection (Figure 5). Significant levels of inhibition of virus replication were observed with each compound when added as early as 2 hours prior to infection. The level of inhibition did not change dramatically as pre-treatment times increased up to 12 hours. All compounds showed some ability to reduce infectivity when added at the time of infection, and 18-β glycyrrhetinic acid, all-trans retinoic acid and mangostin were somewhat effective when added at 1 or 2 hours post-infection. Interestingly, addition of abietic acid post-infection showed a small, but significant increase in infectivity that also was dose-dependent.

Figure 5
figure 5

Time course of inhibition of rotavirus replication. Cells were treated with indicated compounds for various intervals ranging from 12 hr pre-infection to 2 hr post-infection. Each time point was assayed in triplicate and virus was quantified by counting FFU. Open squares are IFN control. Red, 7.5 μg/ml, blue, 5 μg/ml, and green, 2.5 μg/ml The y-axis represents percent inhibition and the x-axis is time of infection. Error bars are standard errors of the means (n = 3).

α-mangostin and 18 β-glycyrrhetinic acid activate NFκB and induce IL-8 secretion

The design of our assay measures inhibition of rotavirus replication but does not distinguish whether antiviral signaling pathways are activated or whether the compounds block specific steps of the virus replication cycle. To test the hypothesis that virus replication was reduced because cell signaling pathways involved in antiviral and inflammatory gene expression were induced by selected compound treatment, NFκB activation and IL-8 secretion was measured following each treatment. α-mangostin and 18 β-glycyrrhetinic acid induced NFκB activation, whereas all-trans retinoic acid and abietic acid did not beyond the levels of the control (Figure 6). The levels of IL-8 expression measured for each compound were consistent with levels of activation of NFκB (Figure 7).

Figure 6
figure 6

NFκB activation in infected and uninfected cells in the presence of selected compounds. Infected or mock infected MA104 cells were treated with indicated compounds at a final concentration of 7.5 μg/ml. Nuclear extracts were prepared 6 hours post-infection and activation of NFκB was assessed and quantified by commercial ELISA. Squares indicate DMSO only treated cultures and triangles indicate compound treated cultures. The y-axis is OD450 nm. The data were analyzed by two-way ANOVA (n = 3).

Figure 7
figure 7

IL-8 expression cells treated with selected compounds. MA104 cells were treated with indicated compounds for 24 hours and IL-8 in the supernatants was measured with a commercial ELISA. ** = p < 0.01, *** = p < 0.001, n = 4.


We designed a MTS assay capable of identifying compounds that reduce rotavirus infectivity, toward a long-term goal of discovering compounds that activate innate immune signaling pathways to reduce the disease impact of acute viral infections. We screened a library consisting of 280 natural products purified from plant extracts, and several compounds were identified that reproducibly inhibited rotavirus replication without cytotoxicity. The assay has been validated statistically, as well as by the observation that compounds selected for further study were purchased from different sources and showed reproducible inhibition of virus replication. In addition, follow-up assays measured reductions in infectivity in response to compound treatment in the standard rotavirus IF assay and the degrees of replication inhibition and dose-dependence correlated with the values obtained in the MTS assay. We calculated a true hit rate for the natural product library of 5% and a false positive rate of ~20%. We performed numerous optimization assays to reduce variability presented by variation in growth properties of our cell line. We also have initiated a screen of a 10,000 compound synthetic chemical library, and after screening ~2500 compounds, our true hit rate is 1.8% (data not shown). These true hit percentages yield a large, but not prohibitive, number of compounds for follow-up studies both in vitro and in animal models. Together the data suggest our assay is an effective platform for screening candidate compounds for antiviral activity in adherent epithelial cells.

In this study, we sought to identify compounds with innate immune modulating effects that resulted in cellular resistance to rotavirus infection. The format of the assay, that is, readout of reduction in infectivity, does not distinguish between compounds that act on antiviral signaling pathways and those that may target specific steps in the virus replication cycle, such as entry or replicase activity. However, two of the four compounds selected for follow-up study activated NFκB and induced IL-8 secretion. Moreover, the majority of compounds called a hit in the screen for which data is available has been previously described to affect inflammatory or anti-inflammatory gene expression or pathogen growth in vitro (Table 1). These observations strongly support the assertion that our assay is appropriate, but not exclusive, for discovery of immune potentiators, as we intended.

There has been a resurgence of interest in natural products as drug candidates for a variety of reasons including increased interest in infectious disease prevention and therapy, a lower percentage of chemical properties that negatively affect permeation and absorption, and the propensity of natural products to act by affecting protein-protein interactions [18]. Important for the studies described here, natural products are known to modulate immune responses and cell signaling pathways. Table 1 lists compounds designated hits in this study, along with some of the reported functions. Compounds were grouped into those chosen for further study (Group A), and those known to stimulate or repress inflammatory responses or innate immune signaling pathways and two with unknown functions (Group B). Also included in Group B are compounds with reported antiviral, antibacterial, or anti-protozoan activity. We have not yet deciphered mechanisms by which these compounds inhibit rotavirus replication in vitro. However, the fact that several of the compounds, for example, abietic acid, genistein, and capsaicin, interfere with NFκB activation and cyclo-oxygenase 2 (COX2) expression is noteworthy (see Table 1 for references). NFκB activation is an important regulator of COX2 expression; COX2 activity and COX-mediated prostaglandin synthesis is necessary for rotavirus infectivity in CaCo-2 intestinal cells [19]. Interestingly, the addition of prostaglandin E2 (PGE2) restored infectivity reduced by the COX inhibitor [19] and three of the compounds we chose for follow-up studies all have an inhibitory effect on either synthesis or release of PGE2 (see Table 1 references). The ability of α-mangostin and 18 β-glycyrrhetinic acid to inhibit rotavirus replication when both compounds activate NFκB is most likely because antiviral states are established upon treatment of the cells with each compound prior to infection. The definitive mechanisms by which the compounds identified in this natural product library screen warrant further investigation.

We intend this MTS assay to serve as a platform for discovery of candidate adjuvants that will be effective against acute viral infections at mucosal surfaces. Rotavirus is an ideal model system for these purposes for several reasons. First, rotaviruses cause gastrointestinal illness in most mammalian species and so their relevance as mucosal pathogens is clear. Second, these viruses are well characterized with respect to structure, antigenicity, and mechanisms of virus replication, and thus an excellent resource for mechanistic follow-up studies is available. Third, rotavirus is promiscuous in its tropism for cultured cell lines, and multiple cell lines of different types and species of origin, including primary cell lines [20], support productive virus replication. The ability to propagate rotavirus in a variety of cell types supports high throughput applications that study general as well as cell-type specific innate immune responses. Fourth, both small and large animal models of natural infection allow relatively rapid evaluation of the efficacy of candidate compounds in vivo. Finally, the fact that rotavirus employs a mechanism to down-regulate antiviral gene expression allows consideration of possible evasion strategies when selecting and testing candidate immune potentiators [21, 22]. Current development efforts include expansion of screening studies to other lytic RNA viruses such as influenza virus, and adaptation of the assay to a fully automated format.


Cells, virus, and rotavirus monoclonal antibodies

MA104 monkey kidney epithelial cells were maintained in M199 medium (MediaTech Cell Grow) supplemented with 5% fetal bovine serum (FBS; Atlanta Biologicals). Isolation and cultivation of G serotype 6 (G6) bovine rotavirus strain NCDV has been described [23]. Monoclonal antibody (MAb) E4 reacts with major structural protein VP6 of most group A rotavirus strains [24]. MAb A6M recognizes VP6 and was generated by immunizing mice with G6 bovine rotavirus strain B641 and screening hybridoma supernatants for antibodies that react with rotavirus specific proteins.

Reagents and chemicals

A library containing 0.5 mg each of 280 natural products purified from plant extracts was purchased from TimTec (TimTec Corporation). Compounds were reconstituted in 500 μl of dimethyl sulfoxide (DMSO) and the library was stored at -80°C. Individual compounds α-mangostin (TimTec or Indofine), 18-β-glycyrrhetinic acid (18-BGA; Fluka), abietic acid (AA; Sigma), and all-trans-retinoic acid (ATRA; Sigma) were reconstituted in DMSO to final stock concentrations of 25 mg/ml.

Recombinant human interferon-α (IFN-α; BioSource International, Inc) was diluted to a concentration of 1 × 105 U/ml in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA). Recombinant human interferon-γ (IFN-γ) and recombinant human interleukin-2 (IL-2; Peprotech Inc) were diluted to 2 × 106 U/ml and 10 μg/ml, respectively, in PBS.

Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L), HRP-conjugated goat anti-mouse IgG (H+L) F(ab')2 fragments, and FITC-conjugated goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories.

Screening assay

Compound treatments and virus infections

Working stock solutions of each compound from the library were prepared to twice the desired final concentrations of 20 μg/ml, 10 μg/ml, and 2 μg/ml in serum-free M199. Working stock solutions of assay controls consisted of 2%, 1% DMSO, and 0.2% DMSO, a mixture of 200 U/ml each of IFN-α and IFN-γ, and 2 ng/ml of IL-2.

MA104 cells were cultured to confluence in 96-well black-walled plates (Costar). The culture media was decanted and replaced with 50 μl of M199. 50 μl of 2X control and experimental stock solutions were added to respective wells, in triplicate, and plates were incubated for 4 hours at 37°C. Following the 4 hour incubation, the contents of each plate were removed and 8.9 × 105 ffu/well of trypsin-activated NCDV in 0% M199 was added to appropriate wells. Mock infected wells received 50 μl of 0% M199. 50 μl of fresh 2X control and experimental compounds were added and at 18 hours post-infection, the cells were fixed for 10 minutes with 80% acetone.

Cell-based ELISA

The wells were blocked for one hour at room temperature with 100 μl of PBS containing 3% bovine serum albumin (w/v) and 0.05% Tween-20. The plates were washed one time with 400 μl of wash buffer consisting of PBS and 0.05% Tween 20. 50 μl of 20 μg/ml A6M or E4 (1:25 hybridoma supernatant) in PBS containing 0.05% Tween 20 and 0.5% dry milk was added to the wells and incubated for one hour at room temperature. The plates were washed four times with wash buffer, then 50 μl of a 1:500 dilution of HRP-conjugated F(ab')2 in 0.5% Blotto was added and the plates were incubated for one hour at room temperature. Following a final wash, 100 μl of BM Chemiluminescence ELISA Substrate (Roche Diagnostics) was added and reactions were allowed to proceed for 4 minutes to reach a steady state of enzymatic activity. Signals were measured on a ThermoElectron Fluroskan (ThermoElectron Cooperation) with an integration time of 1,000 ms.

Cell viability assay

Cell viability assays for compound toxicity were set up similar to compound screening except cells were not infected over the course of the experiments. Cell viability was measured with the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to instructions provided by the manufacturer.

Statistics and criteria for "hit" designation

Compounds that showed a greater than 60% decrease in signal at 5 μg/ml when compared to the 0.5% DMSO control, and p < 0.05 as determined by a one-tailed student's t test, were selected for secondary screening. Additionally, compounds that showed a greater than 90% decrease in signal when compared to the 1% DMSO control and p < 0.05 also were selected for further screening. Compounds that showed a greater than 10% decrease in signal and a p < 0.05 in the cell viability assay were determined to be toxic.

Plate uniformity assessment

The recommendations of the National Institutes of Health Chemical Genomics Center's Assay Guidance Manual Version 4.1 for plate uniformity assessments were followed. Three separate experiments consisting of three plates each were performed on three different days. Confluent MA104 cells were pretreated for 4 hours with 100 μl of either media or a mixture of 100 U/ml each of IFN-α and IFN-γ. All media was serum free and contained 1% DMSO.

The pre-treatment media was decanted and the cells were infected with 50 μl of 8.9 × 105 pfu/ml of trypsin-activated NCDV. Mock infected wells were treated with 50 μl of serum free media. 50 μl of fresh 2X treatment media containing 2% DMSO was added to the respective wells and the plates were incubated for 18 hours. The plates were fixed, labeled and quantified according to the assay procedure described above. All calculations were performed using the Assay Guidance Manual's spreadsheet [14].

Immunofluorescent focus assay

Immunofluorescent assays (IF) for rotavirus infectivity were performed as previously described [24]. Cells were cultured in 96-well plates and were mock infected or infected with NCDV at approximately 150 ffu/well. Pre- or post-treatment with compounds was performed for the indicated times as described above. 18 hours post-infection, the cells were fixed for 10 minutes with 80% acetone. Incubations with primary and secondary antibody were as described above, except the secondary antibody was FITC-conjugated goat anti-mouse IgG. Fluorescent foci were counted by microscopy (Nikon Eclipse TE300).

NFκB and IL-8 assays

MA104 cells in 100 × 20 mm culture dishes were treated with 7.5 μg/ml of selected compounds in serum-free M199 containing 0.03% DMSO. The effects of the presence of virus on NFκB activation and IL-8 secretion in the assays was tested by infecting cells with trypsin-activated NCDV at an moi of 10 pfu/cell at the time of compound treatment. In all cases, incubation periods were 6 hours.

NF-κB activation was quantified with TransAM NF-κB p50 Transcription Factor Assay Kit (Active Motif). Nuclear extracts were prepared with CelLytic NuCLEAR Extraction Kit (Sigma) following the manufacturer's protocol. Nuclear protein concentration was determined with the Dc Protein Assay (Bio-Rad Laboratories), and 20 μg of nuclear protein was used in the assay. Statistical analysis of the data was performed by two-factor ANOVA.

IL-8 secretion was measured with the Quantikine Human IL-8 system (R & D Systems) following the manufacturer's protocol. Compound treatments and infections were performed as described above, except that the incubation periods were extended to 24 hours. The data were analyzed by student's t test with a threshold of significance set to p < 0.001.


  1. Hackett CJ: Innate immune activation as a broad-spectrum biodefense strategy: prospects and research challenges. J Allergy Clin Immunol 2003, 112: 686-694. 10.1016/S0091-6749(03)02025-6

    Article  CAS  PubMed  Google Scholar 

  2. Pashine A, Valiante NM, Ulmer JB: Targeting the innate immune response with improved vaccine adjuvants. Nat Med 2005, 11: S63-S68. 10.1038/nm1210

    Article  CAS  PubMed  Google Scholar 

  3. Amlie-Lefond C, Paz DA, Connelly MP, Huffnagle GB, Whelan NT, Whelan HT: Innate immunity for biodefense: a strategy whose time has come. J Allergy Clin Immunol 2005, 116: 1334-1342. 10.1016/j.jaci.2005.08.048

    Article  CAS  PubMed  Google Scholar 

  4. Johnston PA, Johnston PA: Cellular platforms for HTS: three case studies. Drug Discov Today 2002, 7: 353-363. 10.1016/S1359-6446(01)02140-7

    Article  CAS  PubMed  Google Scholar 

  5. O'Boyle DR, Nower PT, Lemm JA, Valera L, Sun JH, Rigat K, Colonno R, Gao M: Development of a cell-based high-throughput specificity screen using a hepatitis C virus-bovine viral diarrhea virus dual replicon assay. Antimicrob Agents Chemother 2005, 49: 1346-1353. 10.1128/AAC.49.4.1346-1353.2005

    Article  PubMed Central  PubMed  Google Scholar 

  6. Blair WS, Isaacson J, Li X, Cao J, Peng Q, Kong GF, Patick AK: A novel HIV-1 antiviral high throughput screening approach for the discovery of HIV-1 inhibitors. Antiviral Res 2005, 65: 107-116. 10.1016/j.antiviral.2004.11.001

    Article  CAS  PubMed  Google Scholar 

  7. Adelson ME, Pacchia AL, Kaul M, Rando RF, Ron Y, Peltz SW, Dougherty JP: Toward the development of a virus-cell-based assay for the discovery of novel compounds against human immunodeficiency virus type 1. Antimicrob Agents Chemother 2003, 47: 501-508. 10.1128/AAC.47.2.501-508.2003

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Ivens T, Eynde CV, Acker KV, Nijs E, Dams G, Bettens E, Ohagen A, Pauwels R, Hertogs K: Development of a homogeneous screening assay for automated detection of antiviral agents active against severe acute respiratory syndrome-associated coronavirus. J Virol Methods 2005, 129: 56-63. 10.1016/j.jviromet.2005.05.010

    Article  CAS  PubMed  Google Scholar 

  9. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI: Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 2003, 9: 565-572.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Ward RL: Rotavirus vaccines: is the second time the charm? Curr Opin Investig Drugs 2005, 6: 798-803.

    PubMed  Google Scholar 

  11. Knowlton DR, Spector DM, Ward RL: Development of an improved method for measuring neutralizing antibody to rotavirus. J Virol Methods 1991, 33: 127-134. 10.1016/0166-0934(91)90013-P

    Article  CAS  PubMed  Google Scholar 

  12. Iversen PW, Eastwood BJ, Sittampalam GS, Cox KL: A comparison of assay performance measures in screening assays: signal window, Z' factor, and assay variability ratio. J Biomol Screen 2006, 11: 247-252. 10.1177/1087057105285610

    Article  CAS  PubMed  Google Scholar 

  13. Zhang JH, Chung TD, Oldenburg KR: A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 1999, 4: 67-73. 10.1177/108705719900400206

    Article  PubMed  Google Scholar 

  14. []

  15. Nakatani K, Yamakuni T, Kondo N, Arakawa T, Oosawa K, Shimura S, Inoue H, Ohizumi Y: gamma-Mangostin inhibits inhibitor-kappaB kinase activity and decreases lipopolysaccharide-induced cyclooxygenase-2 gene expression in C6 rat glioma cells. Mol Pharmacol 2004, 66: 667-674. 10.1124/mol.104.002626

    Article  CAS  PubMed  Google Scholar 

  16. Takahashi N, Kawada T, Goto T, Kim CS, Taimatsu A, Egawa K, Yamamoto T, Jisaka M, Nishimura K, Yokota K, Yu R, Fushiki T: Abietic acid activates peroxisome proliferator-activated receptor-gamma (PPARgamma) in RAW264.7 macrophages and 3T3-L1 adipocytes to regulate gene expression involved in inflammation and lipid metabolism. FEBS Lett 2003, 550: 190-194. 10.1016/S0014-5793(03)00859-7

    Article  CAS  PubMed  Google Scholar 

  17. Kolla V, Lindner DJ, Xiao W, Borden EC, Kalvakolanu DV: Modulation of interferon (IFN)-inducible gene expression by retinoic acid. Up-regulation of STAT1 protein in IFN-unresponsive cells. J Biol Chem 1996, 271: 10508-10514. 10.1074/jbc.271.18.10508

    Article  CAS  PubMed  Google Scholar 

  18. Koehn FE, Carter GT: The evolving role of natural products in drug discovery. Nat Rev Drug Discov 2005, 4: 206-220. 10.1038/nrd1657

    Article  CAS  PubMed  Google Scholar 

  19. Rossen JW, Bouma J, Raatgeep RH, Buller HA, Einerhand AW: Inhibition of cyclooxygenase activity reduces rotavirus infection at a postbinding step. J Virol 2004, 78: 9721-9730. 10.1128/JVI.78.18.9721-9730.2004

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Buckner D, Wilson S, Kurk S, Hardy M, Miessner N, Jutila MA: Use of Early Passage Fetal Intestinal Epithelial Cells in Semi-high-throughput Screening Assays: An Approach to Identify New Innate Immune System Adjuvants. J Biomol Screen 2006.

    Google Scholar 

  21. Graff JW, Mitzel DN, Weisend CM, Flenniken ML, Hardy ME: Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1. J Virol 2002, 76: 9545-9550. 10.1128/JVI.76.18.9545-9550.2002

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Barro M, Patton JT: Rotavirus nonstructural protein 1 subverts innate immune response by inducing degradation of IFN regulatory factor 3. Proc Natl Acad Sci USA 2005, 102: 4114-4119. 10.1073/pnas.0408376102

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Woode GN, Kelso NE, Simpson TF, Gaul SK, Evans LE, Babiuk L: Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves. J Clin Microbiol 1983, 18: 358-364.

    PubMed Central  CAS  PubMed  Google Scholar 

  24. Zheng SL, Woode GN, Melendy DR, Ramig RF: Comparative studies of the antigenic polypeptide species VP4, VP6, and VP7 of three strains of bovine rotavirus. J Clin Microbiol 1989, 27: 1939-1945.

    PubMed Central  CAS  PubMed  Google Scholar 

  25. Pompei R, Flore O, Marccialis MA, Pani A, Loddo B: Glycyrrhizic acid inhibits virus growth and inactivates virus particles. Nature 1979, 281: 689-690. 10.1038/281689a0

    Article  CAS  PubMed  Google Scholar 

  26. Fernandez MA, Tornos MP, Garcia MD, de las HB, Villar AM, Saenz MT: Anti-inflammatory activity of abietic acid, a diterpene isolated from Pimenta racemosa var. grissea. J Pharm Pharmacol 2001, 53: 867-872. 10.1211/0022357011776027

    Article  CAS  PubMed  Google Scholar 

  27. Hamamoto S, Fukuda R, Ishimura N, Rumi MA, Kazumori H, Uchida Y, Kadowaki Y, Ishihara S, Kinoshita Y: 9-cis retinoic acid enhances the antiviral effect of interferon on hepatitis C virus replication through increased expression of type I interferon receptor. J Lab Clin Med 2003, 141: 58-66. 10.1067/mlc.2003.8

    Article  CAS  PubMed  Google Scholar 

  28. Matsumoto K, Akao Y, Yi H, Ohguchi K, Ito T, Tanaka T, Kobayashi E, Iinuma M, Nozawa Y: Preferential target is mitochondria in alpha-mangostin-induced apoptosis in human leukemia HL60 cells. Bioorg Med Chem 2004, 12: 5799-5806. 10.1016/j.bmc.2004.08.034

    Article  CAS  PubMed  Google Scholar 

  29. Milo GE Jr, Srivastava BI: Effect of cytokinins on tobacco mosaic virus production in tobacco pith tissue cultures. Virology 1969, 39: 621-623. 10.1016/0042-6822(69)90118-4

    Article  CAS  PubMed  Google Scholar 

  30. De Clercq E, Nuwer MR, Merigan TC: The role of interferon in the protective effect of a synthetic double-stranded polyribonucleotide against intranasal vesicular stomatitis virus challenge in mice. J Clin Invest 1970, 49: 1565-1577.

    Article  CAS  PubMed  Google Scholar 

  31. Kessel RW, Braun W: Endotoxin-enhanced host resistance: reversal by kinetin riboside. Nature 1966, 211: 1001-1002. 10.1038/2111001a0

    Article  CAS  PubMed  Google Scholar 

  32. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fukami Y: Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 1987, 262: 5592-5595.

    CAS  PubMed  Google Scholar 

  33. Kim HP, Son KH, Chang HW, Kang SS: Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004, 96: 229-245. 10.1254/jphs.CRJ04003X

    Article  CAS  PubMed  Google Scholar 

  34. Singh S, Natarajan K, Aggarwal BB: Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a potent inhibitor of nuclear transcription factor-kappa B activation by diverse agents. J Immunol 1996, 157: 4412-4420.

    CAS  PubMed  Google Scholar 

  35. Geppetti P, Trevisani M: Activation and sensitisation of the vanilloid receptor: role in gastrointestinal inflammation and function. Br J Pharmacol 2004, 141: 1313-1320. 10.1038/sj.bjp.0705768

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Mensah JL, Lagarde I, Ceschin C, Michel G, Gleye J, Fouraste I: Antibacterial activity of the leaves of Phyllanthus discoideus. J Ethnopharmacol 1990, 28: 129-133. 10.1016/0378-8741(90)90069-6

    Article  CAS  PubMed  Google Scholar 

  37. Weenen H, Nkunya MH, Bray DH, Mwasumbi LB, Kinabo LS, Kilimali VA, Wijnberg JB: Antimalarial compounds containing an alpha, beta-unsaturated carbonyl moiety from Tanzanian medicinal plants. Planta Med 1990, 56: 371-373.

    Article  CAS  PubMed  Google Scholar 

  38. Smith E, Williamson E, Zloh M, Gibbons S: Isopimaric acid from Pinus nigra shows activity against multidrug-resistant and EMRSA strains of Staphylococcus aureus. Phytother Res 2005, 19: 538-542. 10.1002/ptr.1711

    Article  CAS  PubMed  Google Scholar 

  39. Imaizumi Y, Sakamoto K, Yamada A, Hotta A, Ohya S, Muraki K, Uchiyama M, Ohwada T: Molecular basis of pimarane compounds as novel activators of large-conductance Ca(2+)-activated K(+) channel alpha-subunit. Mol Pharmacol 2002, 62: 836-846. 10.1124/mol.62.4.836

    Article  CAS  PubMed  Google Scholar 

  40. Hehner SP, Hofmann TG, Droge W, Schmitz ML: The antiinflammatory sesquiterpene lactone parthenolide inhibits NF-kappa B by targeting the I kappa B kinase complex. J Immunol 1999, 163: 5617-5623.

    CAS  PubMed  Google Scholar 

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This work was supported by DoD contract W9113M-04-1-0010, USDA NRI/CGP 02657 to MEH, the Montana Agriculture Experiment Station, and PHS grant P20 RR020185. ADB gratefully acknowledges financial support from the Arnold and Mabel Beckman Foundation administered through the MSU Beckman Scholars Program.

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Correspondence to Michele E Hardy.

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The author(s) declare that they have no competing interests.

Authors' contributions

MES established the assay, performed most of the experiments and assisted in preparation of the manuscript. ADB performed a significant number of screening assays and data analysis, and participated in manuscript preparation. JWG purified and characterized virus stocks, assisted in assay development and in manuscript preparation. MAJ contributed to study design, assay development, data analysis and manuscript preparation. MEH conceived of the study, participated in design and is responsible for study oversight.

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Shaneyfelt, M.E., Burke, A.D., Graff, J.W. et al. Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay. Virol J 3, 68 (2006).

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