Time course of inhibition of rotavirus replication. Cells were treated with indicated compounds for various intervals ranging from 12 hr pre-infection to 2 hr post-infection. Each time point was assayed in triplicate and virus was quantified by counting FFU. Open squares are IFN control. Red, 7.5 μg/ml, blue, 5 μg/ml, and green, 2.5 μg/ml The y-axis represents percent inhibition and the x-axis is time of infection. Error bars are standard errors of the means (n = 3).