Kinetics of pulmonary immune cells, antibody responses and their correlations with the viral clearance of influenza A fatal infection in mice
- Jin Lv†1,
- Yanhong Hua†3,
- Dan Wang1,
- Aofei Liu1,
- Juan An1,
- Aimin Li1,
- Yanfeng Wang1,
- Xiliang Wang2Email author,
- Na Jia2Email author and
- Qisheng Jiang1Email author
© Lv et al.; licensee BioMed Central Ltd. 2014
Received: 13 November 2013
Accepted: 13 March 2014
Published: 26 March 2014
Fatal influenza A virus infection is a major threat to public health throughout the world. Lung macrophages and neutrophils have critical roles for both the pathogenesis and viral clearance of fatal viral infections. These are complicated by the interaction of innate immunity and adaptive immunity against viral infection. In this study, we investigated the overall kinetics of lung macrophages, neutrophils, CD4+T cells, CD8+T cells, CD38+ cells, and CD138+ cells, the levels of antibody and cytokine responses, both in the early and late phases of fatal infection with A/PR/8/34 (H1N1) virus in mice. The changes in lung viral load were also evaluated. We found that pulmonary macrophages and neutrophils both accumulated in the early and late phases of fatal infections and they positively correlated with the lung and serum antibody titers, and negatively correlated with the viral load locally. The secretion of IL-6 might relate to high numbers of macrophages and neutrophils in the early infection. The work implies that pulmonary macrophages, neutrophils and the antibody response all have an essential role in virus elimination of fatal influenza A viral infection. These findings may have implications for the development of prophylactic and therapeutic strategies in fatal influenza A viral infection. Further evaluation of the cooperation among macrophages, neutrophils and antibody responses in eliminating the virus with fatal infection is needed.
Influenza is an acute epidemic respiratory disease that results in a high rate of mortality in human beings, especially among the elderly and children. A large number of deaths due to influenza are reported every year around the world [1, 2]. Clinically, influenza A virus is the most important virus among the three types of the influenza virus. Influenza A viruses belong to the family Orthomyxoviridae. On the basis of the antigenicity of their haemagglutinin (HA) and neuraminidase (NA) molecules, they are classified into 16 HA subtypes (H1–H16) and 9 NA subtypes (N1–N9). The point mutations and reassortment events of the viral genomes contribute to the emergence of new variants or strains with epidemic or pandemic potential . Influenza A viruses have caused several pandemics during the last century, and continue to cause epidemics annually. The pandemic of 1918–1919 killed as many as 50 million people worldwide [4, 5]. In 2009, a novel swine-origin influenza virus capable of rapid human transmission was reported. As of 4 April 2010, worldwide more than 213 countries and overseas territories or communities have reported laboratory confirmed cases of pandemic influenza H1N1 2009, including over 3200 deaths . The recent report on the drug resistance to oseltamivir phosphate capsules  and H7N9 outbreak in China  have made the prevention and control of pandemic influenza more difficult. Therefore, understanding the mechanisms of increased pathogenicity of fatal influenza A viral infection is critical to optimize antiviral treatment strategies and control potential pandemics.
The uncontrolled and aberrant activation of the innate immune system has been implicated in the mice model of fatal influenza A viral infection [9–11]. A significantly rapid cell recruitment of macrophages and neutrophils into the lungs was assumed to have a role in the pathogenesis associated with H5N1 highly pathogenic avian influenza virus infection (HPAI) . In addition, macrophages and neutrophils were associated with the increased secretion of some cytokine and chemokines , and increased levels of cytokines are suggested to mediate influenza A infection signs [14, 15]. In addition, they may have a role in the severe symptoms of fatal HPAI H5N1 influenza virus infection [16–19]. However, inhibition of the cytokine response cannot protect against the lethal influenza A infection , and neutrophil or macrophage depletion in the early stage of infection has not had a significant effect on the outcome . These findings have suggested complicated biological effects of macrophages and neutrophils in the fatal influenza A viral infection. In addition, innate immune cells such as macrophages and neutrophils, are the targets of influenza A viruses . The direct infection of macrophages and neutrophils may seriously compromise the adaptive immune response.
The mouse model is very useful in the study of influenza virus pathogenesis, especially of the pneumonia by fatal infection, because the immune response and the correlations between these immune parameters in the lung can be monitored and evaluated directly. Influenza A/PR/8/34 H1N1 virus (PR8) is a mouse-adapted influenza strain, which induced the destruction of type II pneumocytes in alveoli in the mice [21, 22]. In addition, several researchers used PR8 as the backbone virus to generate attenuated epidemic influenza vaccines [23–26]. Therefore, a detailed description on immune responses of PR8 fatal infection in the lung of mice could both contribute to the pathogenesis understanding and provide the useful data for comparison with reassortant influenza virus vaccine with PR8 backbone. In this study, we employed PR8 viruses to investigate the kinetics of innate and adaptive cellular immune responses in a mouse model. An overall picture of immune cell activities was obtained, both for the early and late phases of the fatal infection. The local viral load was also measured and its correlations with cellular responses and antibody levels were evaluated.
Materials and methods
Influenza A/PR/8/34(H1N1) virus was kindly provided by Dr. Yuelong-Shu (Chinese Center for Disease Control and Prevention). Ten-day-old embryonated chicken eggs were infected with 0.1 ml of stock virus diluted to 1:1000 in PBS. After incubation for 48 hours at 35~36°C, the allantoic fluid was collected and clarified by centrifugation at 3500gav for 20 min. Virus stocks were aliquoted and stored at −70°C until use. Fifty percent tissue culture infectious dose (TCID50) and 50% egg infectious dose (EID50) titers were determined by serial titration of viruses in Madin-Darby canine kidney (MDCK) cells and eggs, respectively. Titers were calculated by the method of Reed and Muench.
Infection of mice
Female BALB/c mice were purchased from the Institute of Jingfeng Medical Laboratory Animal and were maintained under specific pathogen-free conditions. Five mice in each group were lightly anesthetized by ethylether inhalation and infected by intranasal inoculation (in 25 μl) of 5 × 105, 5 × 104, 5 × 103, 5 × 102, 5 × 101 p.f.u. of viruses to determine the 50% lethal dose (LD50). Plaque assays were performed on MDCK cells to titration of the viruses. Plaque Forming Units (PFU) is a measure of the number of particles capable of forming plaques per unit volume, such as virus particles. It is a functional measurement rather than a measurement of the absolute quantity of particles: viral particles that are defective or which fail to infect their target cell will not produce a plaque and thus will not be counted. One plaque forming unit means a virus or group of viruses which cause a plaque. In the following experiment, 5 × 105 p.f.u. viruses were used to lethal infection of 20 mice per group and eight groups were used. Mice not infected with influenza A/PR/8/34 virus were used as the control group. The animal experiments were approved by the Animal Subjects Research Review Board of the Beijing Institute of Microbiology and Epidemiology and were conducted according to the institution's guidelines for animal husbandry.
Individual body weights from each group were recorded and monitored daily for disease signs and death for 14 d post infection. On days 2, 4, 6, 8, 10, 12 and 14 post infection, 15 mice at each time point were euthanized, and lungs were collected separately and homogenized in 2 mL sterile PBS. After this process, homogenates were frozen separately in sterile tubes at −80°C for later titration of antibody and cytokine detections. For the detection of lung immune cells, five to six mice at each time point post infection were sacrificed by cervical dislocation. The lungs were dissected and placed into cold DMEM. Lung cell preparations were made by passing tissue through a nylon screen. Red blood cells were removed by lysis buffer treatment (BD, biosciences). Cells were counted and resuspended at appropriate concentrations for each particular experiment.
Flow cytometry and cytokine measurement
Single lung cell suspensions were stained with fluorochrome-labeled anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD11c, anti-Ly-6G/C, anti-CD138 and anti-CD38 antibodies (BD Biosciences). Cells were labeled for 45 min at 4°C in staining buffer (PBS with 1%FBS, 0.02% NaN3), washed twice with PBS and fixed overnight at 4°C with 2% paraformaldehyde. Flow cytometry was performed on a FACS Aria flow cytometer (BD Biosciences). IL-6, IL-10, IL-17, IFN-γand IL-4 cytokine protein levels in the lungs were measured by specific enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN, USA).
RT-PCR detection of the viral load
The viral load in the lung tissues was determined by the real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR). We extracted RNA from lung homogenates with Trizol (Invitrogen, USA) reagent. Then, reverse transcriptions targeting at a conserved region of influenza matrix (M) gene were performed, as previously described .
Virus-specific Antibody assays
Influenza-specific serum and lung homogenate antibodies were measured by ELISA, using plates coated with 1 μg/ml per well of purified A/PR/8/34 (H1N1) influenza virus. Briefly, two-fold serial dilutions of sample (1:25 to1:3200 for IgA detection, 1:50 to 1:102400 for IgG detection) were incubated in the plates. Bound Ab was detected with HRP-conjugated goat anti-mouse Abs specific for IgG and IgA, and was developed with TMB (Sigma, USA). Absorbance was read at 450 nm on a Bio-RAD model 550 Microplate Reader. The virus-specific Ab titer was defined as the reciprocal of the highest sample dilution giving an absorbance value greater than twice that of the samples from the negative controls. The titers were gained in duplicate.
Haemagglutination inhibition (HI) assay was performed as previous described [28, 29]. The HI titers were expressed as the reciprocal of the highest dilution that completely inhibited haemagglutination of erythrocyte.
Statistical differences at each time point were determined by one-way ANOVA tests. A Spearman correlation analysis was performed to detect the correlation among viral load and frequencies of different cells, and antibody titers with SPSS19.0. Values of p <0.05 were considered significant.
Comparing the kinetics of lung macrophage and neutrophils with other immune cells in lungs
Comparing the kinetics of pulmonary macrophages and neutrophils with lung antibody responses in lung and serum of mice with fatal influenza A virus infection
Correlations between antibody responses in lungs and serum and pulmonary macrophages and neutrophils in fatal mice infection
Correlations between viral load with pulmonary macrophages, neutrophils and antibody responses in lungs and serum
Correlation analysis between viral load and pulmonary macrophages, neutrophils and antibody levels in serum and lungs in fatal mice infection
Kinetics of cytokines in the lung
Correlations of cytokine responses with macrophages, neutrophils and viral load in lungs
Correlations among the cytokine responses, macrophages, neutrophils and viral load in lungs in fatal mice infection
Macrophages and neutrophils have a complicated role in protecting against high-dose lethal infection of influenza A virus. In this study, the mice were fatally infected with A/PR/8/34 (H1N1) influenza virus. We monitored the kinetics of the macrophages, neutrophils, CD4+T cell, CD8+ T cell, CD138+cell and CD38+cell in the lungs during the viral infection. We found that macrophages and neutrophils accumulated in the lungs, both in the early and late phases of the virus infection. The decreased viral load significantly correlated with the increased macrophages and neutrophils, and antibody levels both in the lung and serum. The present study suggests that macrophages and neutrophils and the antibody responses both have an important role in eliminating of influenza virus locally.
As to both induce the fatal immunopathology in the lung and ensure the enough mice to survive for further experiment, we at first established an appropriate dose of virus. We found 5 × 105 p.f.u. viruses had a decreased lethality in mice than the dose of 5 × 104 and 5 × 103 p.f.u. The reason might be that 5 × 104 p.f.u. could replicate more efficiently than 5 × 105p.f.u.viruses in the upper respiratory tract, thus came higher lethality. We understood it was not common for other influenza viruses, and did not know whether it was correlated with the characteristic of PR8 as a mouse-adapted virus strain, although there was no published article reported the similar experiments.
Observations from de Jong et al.  supported the presence of an inflammatory response role in the pathogenesis of human H5N1 disease. In addition, post-mortem studies in H5N1-infected individuals have not shown predominance of lymphocytes, but rather of macrophages, in pulmonary in-filtrates . Although CD4+T cell and CD8+T cells also have a protective effect during the pathogen infection, especially during high pathogenic avian influenza viruses infection, the protective was also seen in the absence of all T and B cells as well as in the depletion of neutrophils or NK cells . Whereas, depletion of innate lymphoid cells resulted in loss of airway epithelial integrity, diminished lung function and impaired airway remodeling . Furthermore, study by Tate MD  showed that neutrophil depletion early after infection with influenza virus did not alter influenza virus-derived antigen presentation or naïve CD8+T-cell expansion in the secondary lymphoid organs and of trafficking of virus-specific CD8+T cells into the infected pulmonary airways. Instead early neutrophils reduced both the overall magnitude of influenza virus-specific CD8+T cells, together with impaired cytokine production and cytotoxic effector function. In contrast to that, the depletion of macrophages lead to the death of all of the mice even those challenged with a sublethal dose of virus , therefore it was impossible to evaluate the protective effect with removal of macrophages.
The accumulation of macrophages and neutrophils in the early phase also have been observed in mice  and chickens  fatally infected with influenza viruses. In this study, we observed a significant increased frequency of macrophages in the lung at day 4 post infection. We also evaluated the kinetics of macrophages and neutrophils in the late phase infection. When the viral load in the lung significantly decreased at day 10 post infection, the macrophage and neutrophil frequencies were maintained at high levels. Previous research also found that in the late recovery phase, macrophage and neutrophil inhibition led to a marked delay in the elimination of the virus. All these observations suggest that macrophages and neutrophils may contribute to late-phase clearance of influenza viruses . Previous evidence has suggested that influenza A virus infected cells are subjected to apoptosis-dependent phagocytosis and degrade together with the invading virus within phagocytes. It has been speculated that macrophages and neutrophils accumulate in lung tissues and maximize the efficiency of the phagocytic elimination of infected cells .
IL-6 secretion significantly increased early at day 2 post-infection and quickly decreased to the level of the control, suggesting a correlation with the accumulation of macrophage. Suzuki  also reported that IL-6 mRNA was quickly induced at 24 h post-infection, but 8 h later, mRNA levels became dramatically lower. IL-6 concentrations were significantly correlated to the symptoms and signs of influenza A infection in humans [14, 15]. Elevated IL-6 values have been detected in HPAI H5N1 influenza virus infected human cells and mice –[19, 38]. As a multifunctional cytokine expressed by both lymphoid and non-lymphoid cells , IL-6 has a central role in elucidating an innate immune response and directing the transition from innate to adaptive immunity . However, IL-6 cytokine inhibition does not directly protect against death from lethal H5N1 influenza virus infection .
Our study evaluated kinetic responses and correlation of several types of immune cells, cytokines and antibodies in a mouse model, but did not reveal how they were orchestrated in the virus clearance. Further evaluation of the cooperation between macrophages, neutrophils and antibody responses in eliminating the virus with fatal infection is needed.
JL, YHH, XLW, QSJ and NJ conceived and designed the study. Experiments were performed by JL, YHH, DW, and AFL. Data were analysed by JL, AJ, AML, YFW, and NJ. JL and NJ participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
We would like to thank Mr. Shijia-Pei (Cellular Technology Ltd.) for ELISPOT reading and Liangyan-Zhang for Flow cytometry measurement. This study was supported by the grants of National Natural Science Foundation of China (81172130, 81301662).
- CDC: Emergence of avian influenza A(H7N9) virus causing severe human illness - China, February-April 2013. MMWR Morb Mortal Wkly 2013, 62: 366-371.Google Scholar
- Wain-Hobson S: Pandemic influenza viruses: time to recognize our inability to predict the unpredictable and stop dangerous gain-of-function experiments. EMBO Mol Med 2013, 5: 1637-1641. 10.1002/emmm.201303475PubMedPubMed CentralView ArticleGoogle Scholar
- Li W, Shi W, Qiao H, Ho SY, Luo A, Zhang Y, Zhu C: Positive selection on hemagglutinin and neuraminidase genes of H1N1 influenza viruses. Virol J 2011, 8: 183. 10.1186/1743-422X-8-183PubMedPubMed CentralView ArticleGoogle Scholar
- Reid AH, Taubenberger JK, Fanning TG: The 1918 Spanish influenza: integrating history and biology. Microbes Infect 2001, 3: 81-87. 10.1016/S1286-4579(00)01351-4PubMedView ArticleGoogle Scholar
- Seynaeve G: Influenza pandemic: a perspective. Prehosp Disaster Med 2009, 24: 473-477.PubMedView ArticleGoogle Scholar
- Schnitzler SU, Schnitzler P: An update on swine-origin influenza virus A/H1N1: a review. Virus Genes 2009, 39: 279-292. 10.1007/s11262-009-0404-8PubMedView ArticleGoogle Scholar
- Dixit R, Khandaker G, Ilgoutz S, Rashid H, Booy R: Emergence of oseltamivir resistance: control and management of influenza before, during and after the pandemic. Infect Disord Drug Targets 2013, 13: 34-45. 10.2174/18715265112129990006PubMedView ArticleGoogle Scholar
- World Health Organization: Number of confirmed human cases of avian influenza A ( H7N9 ) reported to WHO. : WHO/HO; 2013.Google Scholar
- Kobasa D, Jones SM, Shinya K, Kash JC, Copps J, Ebihara H, Hatta Y, Kim JH, Halfmann P, Hatta M, Kim JH, Halfmann P, Hatta M, Feldmann F, Alimonti JB, Fernando L, Li Y, Katze MG, Feldmann H, Kawaoka Y: Aberrant innate immune response in lethal infection of macaques with the 1918 influenza virus. Nature 2007, 445: 319-323. 10.1038/nature05495PubMedView ArticleGoogle Scholar
- Nakajima N, Van Tin N, Sato Y, Thach HN, Katano H, Diep PH, Kumasaka T, Thuy NT, Hasegawa H, San LT, Kawachi S, Liem NT, Suzuki K, Sata T: Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Mod Pathol 2013, 26: 357-369. 10.1038/modpathol.2012.193PubMedView ArticleGoogle Scholar
- Szretter KJ, Gangappa S, Belser JA, Zeng H, Chen H, Matsuoka Y, Sambhara S, Swayne DE, Tumpey TM, Katz JM: Early control of H5N1 influenza virus replication by the type I interferon response in mice. J Virol 2009, 83: 5825-5834. 10.1128/JVI.02144-08PubMedPubMed CentralView ArticleGoogle Scholar
- Perrone LA, Plowden JK, Garcia-Sastre A, Katz JM, Tumpey TM: H5N1 and 1918 pandemic influenza virus infection results in early and excessive infiltration of macrophages and neutrophils in the lungs of mice. PLoS Pathog 2008, 4: e1000115. 10.1371/journal.ppat.1000115PubMedPubMed CentralView ArticleGoogle Scholar
- Tumpey TM, Garcia-Sastre A, Taubenberger JK, Palese P, Swayne DE, Pantin-Jackwood MJ, Schultz-Cherry S, Solorzano A, Van Rooijen N, Katz JM, Basler CF: Pathogenicity of influenza viruses with genes from the 1918 pandemic virus: functional roles of alveolar macrophages and neutrophils in limiting virus replication and mortality in mice. J Virol 2005, 79: 14933-14944. 10.1128/JVI.79.23.14933-14944.2005PubMedPubMed CentralView ArticleGoogle Scholar
- Reemers SS, Van Leenen D, Koerkamp MJ, Van Haarlem D, van de Haar P, Van Eden W, Vervelde L: Early host responses to avian influenza A virus are prolonged and enhanced at transcriptional level depending on maturation of the immune system. Mol Immunol 2010, 47: 1675-1685. 10.1016/j.molimm.2010.03.008PubMedView ArticleGoogle Scholar
- To KK, Hung IF, Li IW, Lee KL, Koo CK, Yan WW, Liu R, Ho KY, Chu KH, Watt CL, Luk WK, Lai KY, Chow FL, Mok T, Buckley T, Chan JF, Wong SS, Zheng B, Chen H, Lau CC, Tse H, Cheng VC, Chan KH, Yuen KY: Delayed clearance of viral load and marked cytokine activation in severe cases of pandemic H1N1 2009 influenza virus infection. Clin Infect Dis 2010, 50: 850-859. 10.1086/650581PubMedView ArticleGoogle Scholar
- Cheng X, Xu Q, Song E, Yang CF, Kemble G, Jin H: The hemagglutinin protein of influenza A/Vietnam/1203/2004 (H5N1) contributes to hyperinduction of proinflammatory cytokines in human epithelial cells. Virology 2010, 406: 28-36. 10.1016/j.virol.2010.06.048PubMedView ArticleGoogle Scholar
- Geiler J, Michaelis M, Sithisarn P, Cinatl J Jr: Comparison of pro-inflammatory cytokine expression and cellular signal transduction in human macrophages infected with different influenza A viruses. Med Microbiol Immunol 2011, 200: 53-60. 10.1007/s00430-010-0173-yPubMedView ArticleGoogle Scholar
- Ng YP, Lee SM, Cheung TK, Nicholls JM, Peiris JS, Ip NY: Avian influenza H5N1 virus induces cytopathy and proinflammatory cytokine responses in human astrocytic and neuronal cell lines. Neuroscience 2010, 168: 613-623. 10.1016/j.neuroscience.2010.04.013PubMedView ArticleGoogle Scholar
- Song BM, Kang YM, Kim HS, Seo SH: Induction of inflammatory cytokines and toll-like receptors in human normal respiratory epithelial cells infected with seasonal H1N1, 2009 pandemic H1N1, seasonal H3N2, and highly pathogenic H5N1 influenza virus. Viral Immunol 2011, 24: 179-187. 10.1089/vim.2010.0125PubMedView ArticleGoogle Scholar
- Salomon R, Hoffmann E, Webster RG: Inhibition of the cytokine response does not protect against lethal H5N1 influenza infection. Proc Natl Acad Sci U S A 2007, 104: 12479-12481. 10.1073/pnas.0705289104PubMedPubMed CentralView ArticleGoogle Scholar
- Yetter RA, Lehrer S, Ramphal R, Small PA Jr: Outcome of influenza infection: effect of site of initial infection and heterotypic immunity. Infect Immun 1980, 29: 654-662.PubMedPubMed CentralGoogle Scholar
- Loosli CG, Stinson SF, Ryan DP, Hertweck MS, Hardy JD, Serebrin R: The destruction of type 2 pneumocytes by airborne influenza PR8-A virus; its effect on surfactant and lecithin content of the pneumonic lesions of mice. Chest 1975, 67: 7S-14S. 10.1378/chest.67.2_Supplement.7SPubMedView ArticleGoogle Scholar
- Harvey R, Guilfoyle KA, Roseby S, Robertson JS, Engelhardt OG: Improved antigen yield in pandemic H1N1 (2009) candidate vaccine viruses with chimeric hemagglutinin molecules. J Virol 2011, 85: 6086-6090. 10.1128/JVI.00096-11PubMedPubMed CentralView ArticleGoogle Scholar
- He Q, Martinez-Sobrido L, Eko FO, Palese P, Garcia-Sastre A, Lyn D, Okenu D, Bandea C, Ananaba GA, Black CM, Igietseme JU: Live-attenuated influenza viruses as delivery vectors for Chlamydia vaccines. Immunology 2007, 122: 28-37. 10.1111/j.1365-2567.2007.02608.xPubMedPubMed CentralView ArticleGoogle Scholar
- Song JM, Lee YJ, Jeong OM, Kang HM, Kim HR, Kwon JH, Kim JH, Seong BL, Kim YJ: Generation and evaluation of reassortant influenza vaccines made by reverse genetics for H9N2 avian influenza in Korea. Vet Microbiol 2008, 130: 268-276. 10.1016/j.vetmic.2008.02.005PubMedView ArticleGoogle Scholar
- Yang C, Skiena S, Futcher B, Mueller S, Wimmer E: Deliberate reduction of hemagglutinin and neuraminidase expression of influenza virus leads to an ultraprotective live vaccine in mice. Proc Natl Acad Sci U S A 2013, 110: 9481-9486. 10.1073/pnas.1307473110PubMedPubMed CentralView ArticleGoogle Scholar
- Jelley-Gibbs DM, Dibble JP, Brown DM, Strutt TM, McKinstry KK, Swain SL: Persistent depots of influenza antigen fail to induce a cytotoxic CD8 T cell response. J Immunol 2007, 178: 7563-7570.PubMedView ArticleGoogle Scholar
- Jia N, Li C, Liu YX, Richardus JH, Feng D, Yang H, Dang RL, Ma YH, Xu XN, Cao WC: Lower cellular immune responses to influenza A (H3N2) in the elderly. J Med Virol 2009, 81: 1471-1476. 10.1002/jmv.21544PubMedView ArticleGoogle Scholar
- Shapiro-Shelef M, Calame K: Regulation of plasma-cell development. Nat Rev Immunol 2005, 5: 230-242. 10.1038/nri1572PubMedView ArticleGoogle Scholar
- De Jong MD, Simmons CP, Thanh TT, Hien VM, Smith GJ, Chau TN, Hoang DM, Chau NV, Khanh TH, Dong VC, Qui PT, Cam BV, Ha do Q, Guan Y, Peiris JS, Chinh NT, Hien TT, Farrar J: Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia. Nat Med 2006, 12: 1203-1207. 10.1038/nm1477PubMedPubMed CentralView ArticleGoogle Scholar
- Peiris JS, Yu WC, Leung CW, Cheung CY, Ng WF, Nicholls JM, Ng TK, Chan KH, Lai ST, Lim WL, Yuen KY, Guan Y: Re-emergence of fatal human influenza A subtype H5N1 disease. Lancet 2004, 363: 617-619. 10.1016/S0140-6736(04)15595-5PubMedView ArticleGoogle Scholar
- Hamada H, Bassity E, Flies A, Strutt TM, Garcia-Hernandez Mde L, McKinstry KK, Zou T, Swain SL, Dutton RW: Multiple redundant effector mechanisms of CD8+ T cells protect against influenza infection. J Immunol 2014, 190: 296-306.View ArticleGoogle Scholar
- Monticelli LA, Sonnenberg GF, Abt MC, Alenghat T, Ziegler CG, Doering TA, Angelosanto JM, Laidlaw BJ, Yang CY, Sathaliyawala T, Kubota M, Turner D, Diamond JM, Goldrath AW, Farber DL, Collman RG, Wherry EJ, Artis D: Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus. Nat Immunol 2011, 12: 1045-1054. 10.1038/ni.2131PubMedPubMed CentralView ArticleGoogle Scholar
- Tate MD, Brooks AG, Reading PC, Mintern JD: Neutrophils sustain effective CD8(+) T-cell responses in the respiratory tract following influenza infection. Immunol Cell Biol 2012, 90: 197-205. 10.1038/icb.2011.26PubMedView ArticleGoogle Scholar
- Suzuki K, Okada H, Itoh T, Tada T, Mase M, Nakamura K, Kubo M, Tsukamoto K: Association of increased pathogenicity of Asian H5N1 highly pathogenic avian influenza viruses in chickens with highly efficient viral replication accompanied by early destruction of innate immune responses. J Virol 2009, 83: 7475-7486. 10.1128/JVI.01434-08PubMedPubMed CentralView ArticleGoogle Scholar
- Fujisawa H: Neutrophils play an essential role in cooperation with antibody in both protection against and recovery from pulmonary infection with influenza virus in mice. J Virol 2008, 82: 2772-2783. 10.1128/JVI.01210-07PubMedPubMed CentralView ArticleGoogle Scholar
- Hashimoto Y, Moki T, Takizawa T, Shiratsuchi A, Nakanishi Y: Evidence for phagocytosis of influenza virus-infected, apoptotic cells by neutrophils and macrophages in mice. J Immunol 2007, 178: 2448-2457.PubMedView ArticleGoogle Scholar
- Cheung CY, Poon LL, Lau AS, Luk W, Lau YL, Shortridge KF, Gordon S, Guan Y, Peiris JS: Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet 2002, 360: 1831-1837. 10.1016/S0140-6736(02)11772-7PubMedView ArticleGoogle Scholar
- Kishimoto T: IL-6: from its discovery to clinical applications. Int Immunol 2010, 22: 347-352. 10.1093/intimm/dxq030PubMedView ArticleGoogle Scholar
- Lauder SN, Jones E, Smart K, Bloom A, Williams AS, Hindley JP, Ondondo B, Taylor PR, Clement M, Fielding C, Godkin AJ, Jones SA, Gallimore AM: Interleukin-6 limits influenza-induced inflammation and protects against fatal lung pathology. Eur J Immunol 2013, 43: 2613-2625. 10.1002/eji.201243018PubMedView ArticleGoogle Scholar
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