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Figure 2 | Virology Journal

Figure 2

From: Kinetics of pulmonary immune cells, antibody responses and their correlations with the viral clearance of influenza A fatal infection in mice

Figure 2

Kinetics of pulmonary immune cells in mice with fatal dose (5 × 105p.f.u.) of A/PR/8/34 (H1N1) virus infection. The frequencies of macrophages (CD11b+/CD11c/Ly6G/c) and neutrophils (CD11b+/CD11c/Ly6G/c+) were determined by appropriate gating within the total lung leukocytes. For determination of the frequencies of CD4+ and CD8+ T cells, the CD3+ T cells were first determined by appropriate gating within the pulmonary lymphocytes, and then CD4+/CD8T cells and CD4/CD8+ T cells were detected by gating within the CD3+ T cells. In the detection of frequency of CD138+ cells and CD38+ cells, B220/CD45R was used to identify different B cell subtypes within the pulmonary lymphocytes, then CD138+ and CD38+ B cells were determined by appropriate gating within the determined B cell subtype, respectively. Mice without infection were used as the control group. Mean lung cell numbers were representative of at least 3 mice lungs per time point group, and error bars indicated the standard deviations. The dotted lines were the control level. *p < 0.05 compared with control group.

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