- Short report
- Open Access
Evolution of African cassava mosaic virus by recombination between bipartite and monopartite begomoviruses
Virology Journal volume 9, Article number: 67 (2012)
Cassava mosaic disease (CMD) is a major constraint on cassava cultivation in Africa. The disease is endemic and is caused by seven distinct cassava mosaic geminiviruses (CMGs), some of them including several variants.
From cassava leaf samples presenting CMD symptoms collected in Burkina Faso, four DNA-A begomovirus components were cloned and sequenced, showing 99.9% nucleotide identity among them. These isolates are most closely related to African cassava mosaic virus (ACMV) but share less than 89% nucleotide identity (taxonomic threshold) with any previously described begomovirus. A DNA-B genomic component, sharing 93% nucleotide identity with DNA-B of ACMV, was also characterized. Since all genomic components have a typical genome organization of Old World bipartite begomoviruses, this new species was provisionally named African cassava mosaic Burkina Faso virus (ACMBFV). Recombination analysis of the new virus demonstrated an interspecies recombinant origin, with major parents related to West African isolates of ACMV, and minor parents related to Tomato leaf curl Cameroon virus and Cotton leaf curl Gezira virus.
This is the first report of an ACMV-like recombinant begomovirus arisen by interspecific recombination between bipartite and monopartite African begomoviruses.
Cassava (Manihot esculenta Crantz), the primary food crop in sub-Saharan Africa, is severely affected by cassava mosaic disease (CMD) with losses estimated to up to 19 millions tons (1.9-2.7 billions dollars) annually . CMD is caused by distinct cassava mosaic geminiviruses (CMGs) (family Geminiviridae: Genus Begomovirus) which are transmitted by the whitefly Bemisia tabaci (Genn.) . Whereas begomoviruses are either bipartite with two genomic components (DNA-A and DNA-B) or monopartite with only a DNA-A-like component, all described CMGs are bipartite. Their two circular single stranded DNA molecules (~2.8 kb each) encode a total of eight viral proteins responsible, among other functions, for replication, transcription enhancement, encapsidation and viral movement (see  for a review).
CMD is endemic in Africa and seven distinct species are found associated with the disease: African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV), East African cassava mosaic Malawi virus (EACMMV), East African cassava mosaic Zanzibar virus (EACMZV) and South African cassava mosaic virus (SACMV) . Several strains of the EACMV species are also described throughout Africa , many of which resulting from inter- or intra-species recombination . Indeed, it was established that begomoviruses are subject to frequent recombination which provide the viruses with a large sequence space and may promote the adaptation to environmental changes and new ecological niches [4–7]. In contrast to other CMGs, including the EACMV-like viruses (EACMV, EACMCV, EACMKV, EACMMV, EACMZV), SACMV, Sri Lanka cassava mosaic virus (SLCMV) and Indian cassava mosaic virus (ICMV), the genomes of ACMV isolates present little genetic variation and do not display evidence of recombination, suggesting that ACMV has evolved as a distinct lineage (reviewed by ).
In West Africa, cassava is frequently cultivated in association with several other crops such as vegetables (tomato, pepper, okra), tubers (cassava, sweet potato, yam), and cereals. In August/September 2008, leaves from cassava plants presenting CMD symptoms were collected from such a field around Ouagadougou (12°23'31.42"N; 1°30'19.40"W; Burkina Faso). Total DNA was extracted using DNeasy® Plant Minikit (Qiagen) and tested for the presence of begomoviruses using polymerase chain reaction (PCR) with degenerate primers representative of either DNA-A  or DNA-B .
Ten samples showing mosaic symptoms were found to be positive for the presence of begomovirus DNA using PCR. The primary results showed the presence of ACMV and EACMV-UG in seven and one samples respectively . From two of those samples (BF127 and BF128), full-length viral genomes were successfully amplified by rolling-circle amplification (RCA) . The amplified DNAs were digested with endonucleases Bam HI, Pst I, Apa I or Nco I and the DNA fragments of the expected size (~2.8 kb) were cloned into pGEM® vectors (Promega Biotech). Cloned genome components were sequenced by Macrogen Inc. (South Korea) by primer walking and genomic components were assembled using the DNAMAN software (version 5.2.9; Lynnon, Quebec, Canada). Four full-length DNA-A nucleotide sequences, one from sample BF127 (BF:Oua:BF127:08) and three from sample BF128 (BF:Oua:BF128A:08; BF:Oua:BF128B:08; BF:Oua:BF128C:08), as well as one full-length DNA-B nucleotide sequence from sample BF127 (BF:BF127:08), were obtained (Table 1). The sequences were deposited in the EMBL database under the accession numbers HE616777 to HE616781.
The cloned genomes showed an organization typical of bipartite begomoviruses from the Old World, i.e. DNA-A components (2768 to 2770 nt each) with two ORFs on the viral strand (AV2/MP and AV1/CP), and four on the complementary-sense strand (AC1/Rep, AC2/TrAP, AC3/REn and AC4) and a DNA-B component (2737 nt) with one ORF on the viral-sense (BV1/MP), and one on the complementary-sense (BC1/NSP). Sequences were aligned with representative isolates of begomoviruses using the Muscle  and ClustalW  alignment tools implemented in the MEGA 5 software . Pairwise sequence comparisons performed with MEGA 5 (with pairwise deletion of gaps) showed that the four genomic DNA-A components are genetically related with 99.9% identity between one another. The four DNA-A sequences displayed highest nucleotide identity (87%) with ACMV-[Côte d'Ivoire:1999] (Accession n° AF259894) and ACMV-[Nigeria:1990] (X17095). The DNA-B sequence displayed 93% nucleotide identity with ACMV-[Nigeria:Ogo:2002] (AJ427911). Based on the species demarcation criterion of the genus Begomovirus (< 89% nucleotide identity for DNA-A) , the begomovirus molecularly characterised in this work could be considered as a novel species, provisionally named African cassava mosaic Burkina Faso virus (ACMBFV).
The putative Rep protein binding site of the four ACMBFV isolates included a single core binding site (GGGGT) with a potential inverted repeat (GGACC), and the corresponding iteron-related domain (IRD) was identified to be MAPPRKFRIN. These features are different of those from previously described ACMV isolates (Iteron: GGAGA and IRD: MRTPRFRIQ or MRTPRFRVQ), but are similar to those found in isolates of Tomato leaf curl Cameroon virus (ToLCCMV) and Cotton leaf curl Gezira virus (CLCuGV), two monopartite begomoviruses [16, 17]. Interestingly, the presence of the same iteron (GGGGT) in the intergenic region of the ACMBFV DNA-B is consistent with its trans-replication by the Rep protein encoded by the ACMBFV DNA-A component.
As these results suggested that the ACMBFV isolates could have a recombinant origin, a recombination analysis was performed on a set of 234 sequences, with 222 sequences representing the whole Old World begomovirus diversity, ten Curtovirus and two Topocuvirus sequences. The data set was retrieved from public sequence databases using taxonomy browser (http://www.ncbi.nlm.nih.gov) on September 2011 and aligned as described above with our sequences. Detection of potential recombinant sequences, identification of likely parental sequences, and localization of possible recombination breakpoints was carried out using RDP , GENECONV , BOOTSCAN , MAXIMUM CHI SQUARE , CHIMAERA , SISTER SCAN  and 3Seq  recombination detection methods as implemented in RDP3 . The analysis was performed so that only triplets of sequences involving at least two CMG sequences were considered. Default settings for the different detection methods and a Bonferroni corrected P-value cut-off of 0.05 where used. Only events detected with 3 methods or more were accepted.
Three distinct recombination events (a, b and c; Figure 1) were detected within the genomic DNA-A of ACMBFV, whereas no recombination was detected within the DNA-B. Recombination event (a) involves a major parent related to the Ivory Coast isolate of the bipartite ACMV (ACMV-[Côte d'Ivoire:1999]), and a minor parent related to the Cameroon isolate of the monopartite ToLCCMV (ToLCCMV-[Cameroon:Buea:Okra:2008]). Event (b) detected in the intergenic region occurred between ACMV-[Nigeria] as a major parent and CLCuGV-[Cameroon:2008], another monopartite begomovirus described in Cameroon on okra as a minor parent. The event (c) was detected into the event (a) between the same major parent and an unknown minor parent (undescribed or already extinct virus).
Maximum likelihood (ML) phylogenetic trees (with 100 bootstrap replicates) were constructed from full DNA components as well as alignments of both parts of the recombination event (a) using PHYML v3.0.  and the model of sequence evolution selection procedure implemented in RDP3 . Trees were visualized using FigTree v1.3.1 software (available at http://tree.bio.ed.ac.uk/software/figtree/). Consistently, phylogenetic analyses performed with the non-recombinant portion of the genome (nt 27-1727 relative to ACMBFV-[BF:Oua:BF127:08]; covering AV2, AV1, AC2 and AC3) placed ACMBFV in the cluster of ACMV (Figure 2A). With the recombinant portion (nt 1728-26 relative to ACMBFV-[BF:Oua:BF127:08]; covering 90% of 3' portion of Rep, entire AC4 and IR) the same analysis placed ACMBFV in a cluster with ToLCCMV-[CM:Bue:Ok:08] (Figure 2B). Taken together, these results confirm the recombinant nature of the ACMBFV, with the majority of the genome (61.5%) derived from a major ACMV parent, while the rest of the genome (38.5%) is related to ToLCCMV-[CM:Bue:Ok:08] and CLCuGV-[CM:08] minor parents. As expected, phylogenetic reconstruction performed with the DNA-A sequences, using GTR + I + G4 as the best fit model of evolution, placed ACMBFV outside the cluster of ACMV isolates, due to its recombinant nature (Figure 3). A similar analysis performed with the DNA-B sequence of ACMBFV under GTR + G4 evolutionary model placed it within the cluster of ACMV isolates (Figure 4). In order to assess its biological features, the infectivity of our recombinant DNA-A and the need of a cognate DNA-B of ACMV type should be investigated in the future.
In conclusion, we describe for the first time an ACMV-like recombinant begomovirus arisen by interspecific recombination between bipartite and monopartite African begomoviruses. Although current data suggest that ACMV has evolved as a distinct lineage, our results prove that the ACMV genotype is also prone to recombination. Our study confirms that West Africa is likely a centre of begomovirus diversity , where recombination seems to play a major role in the evolution of food and vegetable crops infecting begomoviruses.
Legg JP, Fauquet CM: Cassava mosaic geminiviruses in Africa. Plant Mol Biol 2004, 56: 585-599. 10.1007/s11103-004-1651-7
Patil BL, Fauquet CM: Cassava mosaic geminiviruses: actual knowledge and perspectives. Mol Plant Pathol 2009, 10: 685-701. 10.1111/j.1364-3703.2009.00559.x
Bull SE, Briddon RW, Sserubombwe WS, Ngugi K, Markham PG, Stanley J: Genetic diversity and phylogeography of cassava mosaic viruses in Kenya. J Gen Virol 2006, 87: 3053-3065. 10.1099/vir.0.82013-0
Pita JS, Fondong VN, Sangare A, Otim-Nape GW, Ogwal S, Fauquet CM: Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda. J Gen Virol 2001, 82: 655-665.
Owor EB, Martin DP, Shepherd DN, Edema R, Monjane AL, Rybicki EP, Thomson JA, Varsani A: Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. J Gen Virol 2007, 88: 3154-3165. 10.1099/vir.0.83144-0
Varsani A, Shepherd DN, Dent K, Monjane AL, Rybicki EP, Martin DP: A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family. Virol J 2009, 6: 36. 10.1186/1743-422X-6-36
Lefeuvre P, Lett JM, Varsani A, Martin DP: Widely conserved recombination patterns among single-stranded DNA viruses. J Virol 2009, 83: 2697-2707. 10.1128/JVI.02152-08
Delatte H, Martin DP, Naze F, Golbach RW, Reynaud B, Peterschmitt M, Lett JM: South West Indian Ocean Islands tomato begomovirus populations represent a new major monopartite begomovirus group. J Gen Virol 2005, 86: 1533-1542. 10.1099/vir.0.80805-0
Rojas MR, Gilbertson RL, Russel DR, Maxwell DP: Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminivirus. Plant Dis 1993, 77: 340-347. 10.1094/PD-77-0340
Tiendrébéogo F, Lefeuvre P, Hoareau M, Traoré VSE, Barro N, Reynaud B, Traoré AS, Konaté G, Traoré O, Lett JM: Occurrence of East African cassava mosaic virus-Uganda (EACMV-UG) in Burkina Faso. Plant Pathol 2009, 58: 783.
Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata T: A simple method for cloning the complete begomovirus genome using the bacteriophage φ29 DNA polymerase. J Virol Methods 2004, 116: 209-211. 10.1016/j.jviromet.2003.11.015
Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004, 32: 1792-1797. 10.1093/nar/gkh340
Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22: 4673-4680. 10.1093/nar/22.22.4673
Tamura K, Peterson D, Stecher G, Nei M, Kumar S: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28: 2731-2739. 10.1093/molbev/msr121
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X: Geminivirus strain demarcation and nomenclature. Arch Virol 2008, 153: 783-821. 10.1007/s00705-008-0037-6
Argüello-Astorga GR, Ruiz-Medrano R: An iteron-related domain is associated to motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Arch Virol 2001, 146: 1465-1485. 10.1007/s007050170072
Kon T, Gilbertson RL: Two genetically related begomoviruses causing tomato leaf curl disease in Togo and Nigeria differ in virulence and host range but do not require a betasatellite for induction of disease symptoms. Arch Virol, in press. DOI 10.1007/s00705-011-1139-0
Martin D, Rybicki E: RDP: detection of recombination amongst aligned sequences. Bioinformatics 2000, 16: 562-563. 10.1093/bioinformatics/16.6.562
Padidam M, Sawyer S, Fauquet CM: Possible emergence of new geminiviruses by frequent recombination. Virology 1999, 265: 218-225. 10.1006/viro.1999.0056
Martin DP, Posada D, Grandall KA, Williamson C: A modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints. AIDS Res Hum Retroviruses 2005, 21: 98-102. 10.1089/aid.2005.21.98
Maynard SJ: Analyzing the mosaic structure of genes. J Mol Evol 1992, 34: 126-129.
Gibbs MJ, Armstrong JS, Gibbs AJ: Sister-Scanning: a Monte Carlo procedure for assessing signals in recombinant sequences. Bioinformatics 2000, 16: 573-582. 10.1093/bioinformatics/16.7.573
Boni MF, Posada D, Feldman MW: An Exact Nonparametric Method for Inferring Mosaic Structure in Sequence Triplets. Genetics 2007, 176: 1035-1047.
Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P: RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 2010, 26: 2462-2463. 10.1093/bioinformatics/btq467
Guindon S, Lethiec F, Duroux P, Gascuel O: PHYML Online-a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 2005, 33: 557-559. 10.1093/nar/gki352
This study was supported by the following institutions: AIRES-Sud: a programme from the French Ministry of Foreign and European Affairs implemented by the Institut de Recherche pour le Développement (IRD-DSF), CRSBAN/UFR-SVT (University of Ouagadougou), CIRAD, Conseil Régional de La Réunion and European Union (FEDER) and France Embassy in Burkina Faso. We express our gratitude to Mr François GAUDEAU of the Service de Coopération et d'Action Culturelle (SCAC) of the France Embassy in Ouagadougou (BF). The authors wish to thank Nathalie Becker for her comments, critical reviewing, and for revising the English version of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
FT, VSET and OT collected samples. FT, MH, MAH, ADB and JV cloned and sequenced the viruses; FT, PL and JML analysed the data and prepared the manuscript. JML, OT, NB, GK, AST, VSET and BR secured funding for the project's execution, and provided ideas and comments during manuscript preparation. All authors read and approved the final version of the manuscript.
Electronic supplementary material
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Tiendrébéogo, F., Lefeuvre, P., Hoareau, M. et al. Evolution of African cassava mosaic virus by recombination between bipartite and monopartite begomoviruses. Virol J 9, 67 (2012). https://doi.org/10.1186/1743-422X-9-67
- Cassava mosaic disease
- African cassava mosaic virus