Induction of IL-6 and CCL5 (RANTES) in human respiratory epithelial (A549) cells by clinical isolates of respiratory syncytial virus is strain specific
- Ruth Levitz†1,
- Rachel Wattier†3, 4,
- Pamela Phillips1,
- Alexandra Solomon1,
- Jessica Lawler1,
- Isaac Lazar3, 5,
- Carla Weibel3 and
- Jeffrey S Kahn1, 2Email author
© Levitz et al.; licensee BioMed Central Ltd. 2012
Received: 10 February 2012
Accepted: 24 August 2012
Published: 10 September 2012
Respiratory syncytial virus (RSV) is the major respiratory pathogen of infants and young children. During each seasonal epidemic, multiple strains of both subgroup A and B viruses circulate in the community. Like other RNA viruses, RSV genome replication is prone to errors that results in a heterogeneous population of viral strains some of which may possess differences in virulence. We sought to determine whether clinical isolates of RSV differ in their capacity to induce inflammatory cytokines IL-6 and CCL5 (previously known as RANTES [regulated upon activation, normal T-cell expressed and secreted protein]), which are known to be induced in vitro and in vivo in response to RSV, during infection of A549 cells.
Screening of subgroup A and B isolates revealed heterogeneity among strains to induce IL-6 and CCL5. We chose two subgroup B strains, New Haven (NH)1067 and NH1125, for further analysis because of their marked differences in cytokine inducing properties and because subgroup B strains, in general, are less genetically heterogeneous as compared to subgroup A strains. At 12 and 24 hours post infection RSV strains, NH1067 and NH1125 differed in their capacity to induce IL-6 by an order of magnitude or more. The concentrations of IL-6 and CCL5 were dependent on the dose of infectious virus and the concentration of these cytokines induced by NH1125 was greater than that of those induced by NH1067 when the multiplicity of infection of NH1067 used was as much as 10-fold higher than that of NH1125. The induction of IL-6 was dependent on viable virus as infection with UV-inactivated virus did not induce IL-6. The difference in IL-6 induction most likely could not be explained by differences in viral replication kinetics. The intracellular level of RSV RNA, as determined by quantitative RT-PCR, was indistinguishable between the 2 strains though the titer of progeny virus produced by NH1125 was greater than that produced by NH1067 at 16, 24 and 36 hours but essentially equal at 48 and 72 hours. Full genome sequencing of the 2 strains revealed 193 polymorphisms and 4 insertions in NH1067when compared to NH1125 (2 single base insertions in non-coding regions and 2 duplications of 3 and 60 bases in the RSV G gene). Of the polymorphisms, 147 occurred in coding regions and only 30 resulted in amino acid changes in 7 of the RSV genes.
These data suggest that RSV strains may not be homogeneous with regard to pathogenesis or virulence. Identification of the genetic polymorphisms associated with variations in cytokine induction may lead to insights into RSV disease and to the development of effective antiviral agents and vaccines.
Respiratory syncytial virus (RSV) is a major respiratory pathogen of infants and young children as well as the elderly and immunocompromised populations . Clinical isolates can be categorized into 1 of 2 major subgroups of RSV, A or B, by immunological or genetic methods [2, 3]. Within each subgroup, there are several distinct genotypes, a variety of which co-circulate during yearly epidemics [3–5].
The inflammatory response to RSV infection plays a major role in the disease pathogenesis and this inflammation may result in signs of restrictive and obstructive lung disease long after RSV can be detected in respiratory secretions. RSV elicits the production of a variety of cytokines and chemokines during and after infection [6, 7]. Both in vivo and in vitro data suggest that IL-6, a pro-inflammatory cytokine, is a key component of the response of the host to RSV infection. Microarray analysis of RSV-infected human alveolar type II epithelial (A549) cells reveals that IL-6 mRNA was induced ~20-fold 30 minutes after infection . IL-6 is present in the respiratory secretions of RSV-infected individuals and serum levels of IL-6 are increased during RSV infection [9, 10]. The mechanism by which RSV induces IL-6 production is poorly understood. The chemokine CCL5 (previously known as RANTES [regulated upon activation, normal T-cell expressed and secreted protein]) is induced in humans and animal models of RSV and evidence suggests that this molecule plays an important role in pathogenesis [6, 11]. Therefore, exploration of the induction of IL-6 and CCL5 by clinical isolates of RSV may be insightful.
Several groups, including our own, have demonstrated a potential association between viral genotype and severity of illness, suggesting that clinical isolates of RSV may differ in virulence [9, 12–15]. However, the study of strain-specific factors in vivo is complicated by a variety of host factors influencing the severity of infection. In order to eliminate these factors, we chose to study strain differences in a cell culture model system. As such, we screened and plaque purified clinical isolates of RSV and assessed their ability to induce cytokines in human alveolar type II epithelial (A549) cells. The results demonstrate that clinical isolates differ dramatically in their ability to induce certain cytokines in cell culture. These findings suggest that the genetic variability of clinical isolates of RSV may contribute to the severity of infection caused by the virus.
Screening of clinical isolates of RSV for their ability to induce IL-6
Dynamics of replication of clinical isolates NH1067 and NH1125
Induction of cytokines is dose-dependent
Genome sequence comparisons of NH1067 and NH1125
Sequence comparisons of clinical isolates NH1067, NH1125
Length in basesb
Association of genotype and cytokine induction phenotype
RSV elicits the production of a wide variety of cytokines and chemokines during and after infection [6, 7, 20–23]. There is now mounting evidence that the type of immune response to RSV, specifically the spectrum of host cytokine and chemokine production, ultimately affects disease pathogenesis and chronicity . Cytokine expression likely affects the balance between viral clearance and progression of disease. Therefore, there is a critical need to identify viral factors that regulate cytokine expression during RSV infection .
The vast majority of studies into RSV pathogenesis have used laboratory strains or recombinant virus derived from these laboratory strains. These laboratory strains were isolated decades ago and the passage histories of these strains are not known or are poorly documented. Several studies suggest that sole investigation of laboratory strains may not be ideal to define and characterize RSV pathogenesis. For example, Line 19, a potential vaccine strain derived from a subgroup A isolate possesses biological features that differ from the reference strain A2 (isolated in Australia in 1961) . These phenotypic differences have been mapped to polymorphisms in the RSV F gene . Reference strain A2 differs from several clinical isolates in its ability to replicate on primary human bronchial epithelial cells and to induce interferon-inducible protein 10 and CCL5 suggesting that A2 alone may not be the ideal strain to study RSV pathogenesis . Recently, Stokes et al. demonstrated that several clinical isolates differ in their capacity to induce airway mucous production and virulence in mice . Therefore, the study of clinical isolates will likely yield important insights into the fundamental aspects of pathogenesis.
At least 2 RSV proteins have been implicated in the induction of IL-6, RSV F and G. In monocytes, RSV F stimulates IL-6 through interactions with CD14+/TLR4 complex by promoting nuclear translocation of NF-ĸB . IL-6, along with other cytokines, such as IL-1β and IL-8, play an important role in neutrophil and macrophage chemotaxis. The cellular inflammatory response during severe RSV infection is characterized by a preponderance of neutrophils and macrophages, suggesting that the expression of IL-6 at the site of infection is an essential feature in disease progression . Differences in IL-6 induction may explain, in part, differences in severity of illness seen with RSV infection. Indeed, clinical studies of both infants and adults have demonstrated that single nucleotide polymorphisms in the IL-6 promoter (position −174) were associated with a greater severity of illness suggesting a pivotal role of IL-6 in RSV pathogenesis [30, 31]. Our data suggests that variability in the induction of IL-6 may, at least, partially explain differences in the observed severity of human RSV disease associated with infection due to specific RSV genotypes [9, 12–15]. The RSV F protein of NH1067 and NH1125 differs at only 4 amino acid residues (positions 67, 292, 490 and 529). It did not appear that these amino acid changes can account for the differences in induction of IL-6 observed because the specific amino acid residues at these positions did not correlate to induction phenotype when other strains were examined.
Several lines of evidence indicate that the RSV G glycoprotein also plays a role in pathogenesis and can induce IL-6 among other cytokines [32–37]. Monocyte stimulation experiments using recombinant viruses with altered or absent G gene and synthetic peptides, corresponding to regions of the RSV G protein, revealed that portions of the highly conserved cysteine-rich region, specifically amino acids 164–176, inhibit the innate immune response . The CX3C motif (amino acid 182–186) of the RSV G glycoprotein mimics the CX3C chemokine fractalkine, competes for the chemokine-specific receptor and interferes with fractalkine-mediated leukocyte chemotaxis . RSV deficient in the soluble form of G (generated by initiation at the second AUG corresponding to amino acid 48) induced a pro-inflammatory response in A549 cells . Amino acids 164–176 and 182–186 were identical in NH1067 and NH1125. Both strains contained a methionine at position 48. Therefore, these regions of the RSV G gene, although likely important for cytokine induction pathways, cannot account for the differences observed in IL-6 induction between NH1067 and NH1125.
Our data suggest that the differences in the RSV F protein between NH1067 and NH1125 are unlikely to account for the differences in cytokine induction phenotype strongly suggesting that other viral factors, perhaps in other domains of the G protein, may play a role in the differences in cytokine induction observed in the clinical isolates. These factors may not necessarily be viral proteins. A single nucleotide substitution in the transcriptional start signal of the M2 gene, within a non-coding region of the RSV genome  is the major determinant of the temperature-sensitive, attenuated phenotype of a potential vaccine candidate. However, the consensus transcriptional start and stop signals for each gene were identical between NH1067 and NH1125. It appears as though viral transcriptional and/or replication activity rather than binding of virion-associated glycoproteins to cellular receptors is required as UV-inactivated virus failed to induce IL-6, an observation that has been reported elsewhere [40, 41].
RSV causes a wide-spectrum of disease and this may be due, in part, to the differences in biological properties of the infecting virus. This phenomenon has been observed with other viruses. For example, mutations in the HCV NS5A gene are associated with a sustained virological response to interferon therapy . A neurotropic variant of HIV, containing a mutation in the envelope gene, has been identified, which is present at a high frequency in brain tissue in AIDS patients with dementia . Mutations in several genes may account for the increased virulence of the 1918 influenza pandemic strain . Identification of the viral gene(s) involved in the induction of key cytokines, chemokines and inflammatory mediators would potentially lead to targeted antiviral therapies and would be a significant advance in the study of RSV pathogenesis.
This work demonstrates that clinical isolates of RSV are not homogeneous in their ability to induce specific cytokines. Screening of a relatively small number of clinical isolates resulted in the identification of strains, NH1067 and NH1125, with markedly different cytokine-inducing phenotypes. This work demonstrates the utility of investigating clinical isolates, whose naturally-occurring mutations result in distinct phenotypes. These data suggest that further investigation, particularly with the use of primary human cells (airway epithelial cells or PBMCs), of these and other clinical isolates of RSV may yield insights into pathogenesis and virulence.
Viruses and cells
HEp-2 (CCL-23) and A549 (CCL-185) cells were obtained from the American Type Culture Collection (Manassas, VA) and cultivated in EMEM with 10 % fetal bovine serum. HEp-2 cells were used in the initial propagation and titration of the clinical strains; A549 cells were used in the cytokine experiments described below. Clinical isolates were obtained from RSV-infected individuals as described previously from New Haven, Connecticut  and Dallas, Texas. Viruses used in this study were propagated from specimens submitted to the clinical (diagnostic) laboratory (in New Haven, CT and Dallas, TX). All specimens from which viruses were obtained were submitted as part of routine care. Only left over material was used for viral propagation. Collection of specimens from the Clinical Virology Laboratory at Yale-New Haven Hospital was approved by the Yale University Human Investigations Committee. The single isolate from Dallas, Texas was propagated from a de-identified clinical specimen obtained from the Clinical Microbiology Laboratory at Children’s Medical Center, Dallas. Collection and use of clinical isolates followed all institutional requirements and guidelines and was consistent with policies and regulations for the use of patient derived materials.
Isolates were plaque purified 3 times on HEp-2 cells. Working stocks were prepared using 30%-60% (w/v) non-continuous sucrose density centrifugation (SW-28, 28,000 rpm, 4°C for 90 minutes). The virus containing band at the 30%-60% interface was collected, distributed into aliquots, and stored at −80°C. Viral titers were determined using a plaque assay . Plaques were detected with an immunohistochemical staining technique using either goat anti-RSV HRP-conjugated antibody (Fitzgerald Industries International, Acton, MA) or a primary anti-RSV antibody (palivizumab, MedImmune, Gaithersberg, MD) and secondary HRP conjugated anti-human antibody (Jackson ImmunoResearch Laboratories, Inc., West grove, PA). Viruses were inactivated by subjecting viral inoculums to 2 doses of 5,000 joules of ultraviolet (UV) light. UV-inactivation was confirmed by cell culture.
Monolayers of A549 cells were infected with sucrose-purified clinical isolates of RSV at the moi designated in the text. After 90 minutes of infection, the inoculum was removed, the cells were washed with serum-free media and fresh media (F-12 Kaighn’s modification) containing 5% FBS was added to the infected monolayers. For cytokine analysis, supernatants were collected, clarified by centrifugation, snap frozen in liquid nitrogen and stored at −80°C until the specific assay was performed. For the initial screening of clinical isolates, the concentration of IL-6 was determined by ELISA using the DuoSet Human IL-6 ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s specifications. Standard curves with known concentrations of IL-6 were performed for each assay. All samples were analyzed on the same ELISA plate to minimize technical bias and there were at least 3 biological replicates for each measurement. For experiments that measured concentrations of IL-6 and CCL5, BioPlex assays were performed using Bio-Plex Pro™ with conjugated magnetic beads according to the manufacturer’s instructions. Cytokine data were analyzed using Bio-Plex Manager™ version 4.1.1 software.
Real time PCR
Total RNA was extracted from A549 cell monolayers using the RNeasy Mini kit and QIAshredder columns (QIAGEN, Valencia, CA) according to the manufacturer’s recommendations. RNA extracts were treated with RNase-free DNase I (Roche), then reverse transcribed with Moloney murine leukemia virus reverse transcriptase (New England BioLabs) with a primer specific for the genomic (negative-sense) copy of the RSV N gene, 5'-ATGGCTCTTAGCAAAGTC-3'. PCR amplification of cDNA was performed in a reaction mixture containing 5 μL cDNA template, forward (5'-CTGTCATCCAGCAAATACACTATTCA-3') and reverse (5'-GCACATCATAATTGGGAGTGTCA-3') primers at a concentration of 400 nM each, and 12.5 μL iQ SYBR Green Supermix (Bio-Rad) in a total volume of 25 μL. Reaction conditions were as follows: 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. For cDNA standards, RNA was extracted from plaque-titered stocks of the laboratory reference B strain 8/60 using the QIAamp Viral RNA Mini Kit (Qiagen), reverse-transcribed as above, and serially diluted. RSV genomic RNA quantity in each sample was calculated in “pfu equivalents.”
Viral genome sequencing, phylogenetic analysis and real time PCR
Viral RNA was extracted from clarified supernatants or sucrose-purified virus using QIAamp® Viral RNA Mini kit (QIAGEN). Reverse transcriptase and PCR were performed as previously described . Primers used for the sequencing of clinical isolates are listed in Additional file 1. For sequence of the ends of the viral genome, purified viral RNA was circularized by ligation using T4 RNA ligase (Epicentre, Madison, WI) as described elsewhere . Primers were designed (see Additional File 1) to span the region of the ligated ends. The exact nucleotide at the 3’ and 5’ ends were determined by comparison to available genome sequences. DNA sequencing of PCR products was performed by the McDermott Center Sequencing Core using Applied Biosystems Inc. (ABI) Big Dye Terminator 3.1 chemistry and analyzed on ABI capillary instruments. Sequence analysis including alignment and integration of contigs, sequence comparisons and alignments, and phylogenetic analysis were performed using DNASTAR Lasegene 8 software (MegAlign and Seqman).
RW current institution is The University of California San Francisco. IL current institution is Ben Gurion University, Beer Sheva, Israel. AS is an undergraduate student at Cornell University. JL is an undergraduate student at Texas A&M.
This work was supported, in part, by funds from the University of Texas Southwestern Medical Center and Children’s Medical Center Dallas. We are indebted to George Miller, M.D., John Enders Professor of Pediatrics, Epidemiology and Public Health and Molecular Biophysics and Biochemistry, Yale University School of Medicine for his support and critical review of the data. We thank Marie Landry, M.D., David Ferguson and the staff of the Clinical Virology Laboratory, YaleNew Haven Hospital (CT), and Chris Doern, Ph.D, Director, Clinical Microbiology, Children’s Medical Center for their assistance. We thank Benjamin Goldwater for his critical review of the manuscript.
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