- Open Access
Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution
© Yutin et al; licensee BioMed Central Ltd. 2009
- Received: 27 October 2009
- Accepted: 17 December 2009
- Published: 17 December 2009
The Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) comprise an apparently monophyletic class of viruses that infect a broad variety of eukaryotic hosts. Recent progress in isolation of new viruses and genome sequencing resulted in a substantial expansion of the NCLDV diversity, resulting in additional opportunities for comparative genomic analysis, and a demand for a comprehensive classification of viral genes.
A comprehensive comparison of the protein sequences encoded in the genomes of 45 NCLDV belonging to 6 families was performed in order to delineate cluster of orthologous viral genes. Using previously developed computational methods for orthology identification, 1445 Nucleo-Cytoplasmic Virus Orthologous Groups (NCVOGs) were identified of which 177 are represented in more than one NCLDV family. The NCVOGs were manually curated and annotated and can be used as a computational platform for functional annotation and evolutionary analysis of new NCLDV genomes. A maximum-likelihood reconstruction of the NCLDV evolution yielded a set of 47 conserved genes that were probably present in the genome of the common ancestor of this class of eukaryotic viruses. This reconstructed ancestral gene set is robust to the parameters of the reconstruction procedure and so is likely to accurately reflect the gene core of the ancestral NCLDV, indicating that this virus encoded a complex machinery of replication, expression and morphogenesis that made it relatively independent from host cell functions.
The NCVOGs are a flexible and expandable platform for genome analysis and functional annotation of newly characterized NCLDV. Evolutionary reconstructions employing NCVOGs point to complex ancestral viruses.
- Gene Repertoire
- Unicellular Eukaryote
- Approximately Unbiased
- Giant Virus
- Phyletic Pattern
The 6 NCLDV families used for the NCVOG construction
Genome size range, kb
Green algae; algal symbionts of paramecia and hydras
Nucleus and cytoplasm
Animals: insects, reptiles, birds, mammals
Asco- and Iridoviridae
Invertebrates and non-mammalian vertebrates
Insects, mainly, Noctuids
Nucleus and cytoplasm
Insects, cold-blooded vertebrates
Nucleus and cytoplasm
Nucleus and cytoplasm(?)
Generally, the NCLDV do not show strong dependence on the host replication or transcription systems for completing their replication . This relative independence of the viruses from the host cells is consistent with the fact that all these viruses encode several conserved proteins that mediate most of the processes essential for viral reproduction. These key proteins include DNA polymerases, helicases, and DNA clamps responsible for DNA replication, Holliday junction resolvases and topoisomerases involved in genome DNA manipulation and processing, transcription factors that function in transcription initiation and elongation, ATPase pumps for DNA packaging, and chaperones involved in the capsid assembly [5, 6]. Although only 9 genes were found to be conserved in all NCLDV (with sequenced genomes), a considerable number of additional genes are shared by diverse viruses from multiple families. An evolutionary reconstruction using a parsimony approach mapped approximately 40 genes to the putative common ancestor of the NCLDV . Thus, it appears that the ancestral NCLDV already was a complex virus that generally resembled the extant members of this group and was capable of relatively independent reproduction in the cytoplasm of the host cells, the exact identity of the host notwithstanding [6, 8].
The NCLDV share some of the virus hallmark genes  with other large DNA viruses such as herpesviruses and baculoviruses. Examples of such shared hallmark genes include the B-family DNA polymerases, DNA primases, and Superfamily 2 helicases related to herpesvirus origin-binding protein UL9. However, most of the NCLDV share a considerable number of additional genes to the exclusion of other large DNA viruses of eukaryotes. Cases in point include the Superfamily 3 helicase (typically, fused with primase in NCLDV), the packaging ATPase, the disulfide oxidoreductase involved in virion morphogenesis, and more. The existence of these signature NCLDV genes, despite the notable connectivity of the virus world, justifies the classification of the NCLDV as distinct, monophyletic class of viruses [5, 6].
In the last few years, the NCLDV attracted much new attention owing, primarily, to the discovery and genome sequencing of the giant Mimivirus that was isolated from Acanthamoeba. At ~1.2 Mb, the Mimivirus and the closely related Mamavirus possess by far the largest genomes of all known viruses [9–13]. These viruses encompass the full complement of conserved NCLDV genes but also possess numerous genes homologous to genes of cellular organisms including several encoding translation system components. The unexpected discovery of these genes in the mimivirus led to speculation on the origin of the giant viruses from a putative "fourth domain of cellular life" by genome degradation . However, comparison of the mimivirus gene repertoire with those of other NCLDV combined with phylogenetic analysis of both conserved NCLDV genes and the homologs of host genes encoded by the mimivirus indicate that the Mimivirus is a bona fide NCLDV and appears to be related to phycodnaviruses and iridoviruses . The homologs of genes of cellular organisms, in all likelihood, were acquired in the course of evolution of the mimivirus lineage, probably, from a variety of distinct cellular sources; the same process of horizontal acquisition of cellular genes occurred, on a smaller scale, in all other families of the NCLDV [6, 8, 15–18].
Very recently, another giant virus, named Marseillevirus, was isolated from Acanthamoeba. Genome analysis of Marseillevirus indicated that it represents a putative novel family of NCLDV that appears to be distantly related to iridoviruses and ascoviruses . In addition, comparative-genomic analysis revealed probable gene exchange between Marseillevirus and Mimiviruses, emphasizing the role of amoeba as a "melting pot" of NCLDV evolution.
An interesting new perspective on the NCLDV emerged from the rapid progress of metagenomic studies. It turns out that the DNA samples from the Global Ocean Survey contain numerous sequences homologous to genes of all known NCLDV families, except for Poxviridae and Ascoviridae, and possibly, representatives of new families as well [19–23]. Thus, there seems to exist a considerable unexplored diversity of NCLDV that most likely infect various unicellular eukaryotes but, possibly, also marine invertebrates .
As the number of available viral genomes quickly grows, both challenges and the potential of comparative and evolutionary genomics of the NCLDV increase. A pre-requisite of an informative comparative-genomic study of any group of organisms is an accurate delineation of the sets of orthologous genes, that is, genes that evolved from the same gene in the genome of the last common ancestor of the compared genomes [25, 26]. Accurate identification of clusters of orthologous (COGs) is essential both for functional annotation of uncharacterized genes and for evolutionary reconstructions. The COG analysis has been initially applied in a comprehensive manner, to all then available genomes of archaea, bacteria and unicellular eukaryotes [27, 28], but subsequently, with the exponential growth of the collections of sequenced genomes, it became more realistic to derive COGs for compact taxa such as archaea or cyanobacteria [29, 30]. The NCLDV, with their large (on the virus scale) genomes consisting of genes with different degrees of evolutionary conservation, are in need of and amenable to the same approach. Here we describe the construction of clusters of orthologous genes for the NCLDV which we abbreviate as NCVOGs (Nucleo-Cytoplasmic Virus Orthologous Genes) which we intend as a platform for the functional and evolutionary analysis of new NCLDV genomes. We also report some patterns of evolution of the NCLDV that can be inferred from a preliminary analysis of the NCVOGs.
Clusters of orthologous genes for the NCLDV (NCVOGs)
Phylogenies of the core proteins of the NCLDV
As the number of genomes of cellular life available for comparative analysis increases, the set of universal genes, which comprised a small fraction of the genes even in the original COG analysis , continues to shrink [31, 32]; in large part, this is a consequence of non-orthologous displacement whereby the same indispensable function is mediated by unrelated genes in different life forms . Non-orthologous gene displacement as well as lineage-specific gene loss seem to be important in the evolution of the NCLDV as well, the result being that only a few genes are conserved in all viruses of this class. In the present analysis, only 5 NCVOGs included proteins from all 45 analyzed viruses, namely, the major capsid protein (orthologs of vaccinia virus D13 protein), primase-helicase (VV D5), Family B DNA polymerase (VV E9), packaging ATPase (VV A32), and transcription factor (VV A2). Given the previous conclusions on the origin of the NCLDV from a single ancestral virus [5, 6], we sought to reconstruct the phylogeny of the NCLDV by analyzing the phylogenetic trees of these highly universal proteins as well as additional highly conserved proteins. The capsid protein is not suitable for reconstructing NCLDV phylogeny: the sequences of the capsid protein ortholog in poxviruses (VV D13) are extremely divergent, resulting in low information content of the alignment, and other viruses encode multiple paralogs of the capsid protein). The remaining 4 conserved proteins yielded phylogenetic trees with somewhat conflicting topologies (Additional File 2). Assuming that the conflicts were caused by tree construction artifacts rather than genuinely different histories of different core gene of the NCLDV, we employed the consensus tree approach (see Methods for details) to reconstruct the putative NCLDV phylogeny using 10 trees of genes that are represented in all or nearly all of the NCLDV. Specifically, the phylogenies of the following 10 conserved genes contributed to the consensus tree: Superfamily II helicase, A2L-like transcription factor, RNA polymerase A subunit, RNA polymerase B subunit, mRNA capping enzyme, A32-like packaging ATPase, small subunit of ribonucleotide reductase, Myristylated envelope protein, primase-helicase, and DNA polymerase (See Additional File 2).
We further constructed a different type of tree for the NCLDV, one that was based on the comparison of gene repertoires, more specifically, the patterns of representation of viruses in NCVOGs, also known as phyletic patterns . The trees were produced from the 15 × 1445 matrix of subfamily-level phyletic patterns using the neighbor-joining tree reconstruction method and 4 different methods for distance calculation (see Methods for details and Additional File 3). The topologies of these gene content trees were generally compatible with that of the consensus tree (Figure 3), indicating that the evolution of the gene repertoire of the NCLDV, largely, mirrored the evolution of the conserved core genes. However, there was one notable exception to this congruence: in 3 of the 4 gene content trees, Marseillevirus clustered with the Mimiviridae. This similarity of gene repertoires, most probably, stems from the reproduction of these viruses in the same host (Acanthamoeba) where the viruses repeatedly exchanged genes during their evolution .
Conserved genes and reconstruction of the evolution of the NCLDV gene repertoire
Some of the core functions are prone to non-orthologous displacement among the NCLDV, sometimes showing complex evolutionary patterns. A case in point is the DNA ligase that is an essential activity for DNA replication. The previous reconstruction of the ancestral NCLDV gene repertoire tentatively identified the ATP-dependent ligase as an ancestral NCLDV gene [5, 6]. However, entomopoxviruses, mimiviruses, and some of the iridoviruses lack the ATP-dependent ligase and instead encode a distinct NAD-dependent ligase (of apparent bacterial origin) (see Additional Files 1 and 4). Furthermore, some poxviruses, such as Molluscum Contagiosum virus , encode no ligase at all, apparently, as a result of lineage-specific gene loss; in such cases, this essential replication function is probably supplied by a host ligase. The present maximum-likelihood reconstruction mapped both ligases to the ancestral NCLDV genome. However, phylogenetic analysis of the ATP-dependent and NAD-dependent ligases yielded an unexpected result: the NAD-dependent ligases of the NCLDV formed an unequivocally supported clade whereas the ATP-dependent showed different phylogenetic affinities . The conclusion, perhaps, a counterintuitive one is that the NAD-dependent ligase, of bacteriophage or bacterial origin, is the ancestral NCLDV gene that was repeatedly displaced by ATP-dependent ligases in different viral lineages . These findings reveal inherent limitations of reconstructions of ancestral gene repertoires based on patterns of gene presence-absence.
Owing to non-orthologous displacement, some of genes encoding (nearly) essential functions might not have made it to the reconstructed ancestral gene repertoire. An interesting potential case of such missing function is that of phospholipase that is likely to be required for NCLDV morphogenesis as well as for the escape of the virus from the host phagosomes. A large subset of the NCLDV including mimiviruses, Marseillevirus, and some phycodnaviruses and iridovirsues encode a patatin-family phospholipase (Additional File 1) that has been implicated in the pathogen-host interaction of intracellular bacterial parasites such as Legionella . In poxviruses, this phospholipase is missing but there are one or two paralogous genes encoding a distinct enzyme of the phospholipase D family which is part of the virus envelope  and is involved in the formation of virus-specific vesicles in infected cells . It seems plausible that the ancestral NCLDV encoded the patatin-like phospholipase that was subsequently displaced by the unrelated phospholipase D-like enzyme in poxviruses. Similar patterns of non-orthologous gene displacement are likely to involve additional NCLDV genes, emphasizing the inevitable conservative character of the evolutionary reconstruction.
The results of the evolutionary reconstruction indicate that the common ancestor of the NCLDV already was a bona fide virus of this class and, in particular, possessed the same degree of independence of the host cell functions as the extant NCLDV. The NCLDV infect diverse eukaryotes including a wide range of unicellular forms, and moreover, remarkable diversity of the hosts is seen even within some of the NCLDV branches; the relationship between irido-ascoviruses infecting animals and Marseillevirus that reproduces in Acanthamoeba is a case in point (Figures 3 and 4). Thus, it appears most likely that this full-fledged ancestral NCLDV evolved at an early stage of eukaryotic evolution, prior to the divergence of the eukaryotic supergroups, and that the radiation of the branches of the NCLDV was a very early event as well. It is tempting to speculate that this initial radiation of the NCLDV occurred as a "Big Bang-like" event concomitantly with eukaryogenesis , a model similar to that recently elaborated for a completely different group of eukaryotic viruses, the picorna-like superfamily of RNA viruses .
The actual genome size and complexity of the ancestral NCLDV is a wide-open question. Clearly, the 47 genes mapped to the ancestral genome in the present reconstruction comprise only the core of most highly conserved, essential viral genes involved in key functions. Given that the ancestral NCLDVs undoubtedly reproduced in unicellular eukaryotes, and this type of host supports the propagation of extant giant viruses, such as the mimiviruses [13, 24], it cannot be ruled out that already at an early stage of evolution the ancestral NCLDV genome grew highly complex. Thus, the common ancestor of all extant NCLDV even might have been a giant virus.
The goal of this work was to classify the genes from the growing collection of the NCLDV genomes into clusters of probable orthologs and in-paralogs in order to facilitate annotation of newly sequenced viral genomes and analysis of viral evolution. It is our hope that the curated set of NCVOGs will serve these purposes, in particular, with respect to new giant viruses that undoubtedly will be isolated from unicellular eukaryotes in the nearest future. The comparative analysis of the NCLDV genes showed that only 177 of the 1445 NCVOGs include representatives from more than one virus family. An even smaller set of 47 conserved genes was mapped to the common ancestor of the NCLDV by the maximum-likelihood reconstruction. This reconstructed ancestral gene set is robust to the parameters of the reconstruction procedure and does not dramatically differ from the ancestral gene set reconstructed previously on a smaller collection of viral genomes and using a simpler, parsimony method . In particular, the inclusion of representatives of two additional virus families, the Ascoviridae and the putative new family represented by the Marseillevirus, did not result in an erosion of the reconstructed ancestral gene set. However, detailed phylogenetic analysis can lead to some revisions of the ancestral gene set as illustrated by the case of ATP-dependent and NAD-dependent DNA ligases. These caveats notwithstanding, it seems that the reconstruction reflects the gene core of the ancestral NCLDV with a reasonable accuracy and indicates that this virus encoded a complex machinery of replication, expression and morphogenesis that made it relatively independent from host cell functions.
Construction of the NCVOGs
For the construction of the NCVOGs, we used 45 annotated protein sets of Nucleo-Cytoplasmic Large DNA viruses (NCLDV) (see Additional File 6; 5 closely related Orthopoxviruses were not included).
Ankyrin repeat-containing proteins were the most abundant proteins in the data set (~400 proteins, or 3.5% of the data set). Owing to the low sequence complexity of these proteins, they produced large number of false-positive hits during similarity searches. These proteins were removed from the data set prior to clustering.
Multiple alignments of the initial cluster members were constructed using the MUSCLE program . The alignments were used to construct position-specific scoring matrices (PSSM) for a PSI-BLAST search against the NCLDV protein dataset. Hits with e-values below 0.01 were reviewed, and clusters were merged when appropriate.
Clusters were further manually checked and edited using BLASTCLUST http://www.ncbi.nlm.nih.gov/IEB/ToolBox/C_DOC/lxr/source/doc/blast/blastclust.html and RPS-BLAST . As a result of these refinement procedures, 1,445 NCVOGs consisting of 9,261 proteins were obtained.
The NCVOGs were manually annotated on the basis of RPS-BLAST and PSI-BLAST hits of cluster members.
The NCVOGs are available at ftp://ftp.ncbi.nih.gov/pub/wolf/COGs/NCVOG/.
Multiple alignment and phylogenetic tree construction
The sequences for phylogenetic analysis were aligned using MUSCLE . Poorly conserved positions and positions including gaps in more than one-third of the sequences were removed prior to tree computation.
Maximum Likelihood trees (ML) were constructed using TreeFinder , with the estimated site rates heterogeneity and the WAG (Whelan and Goldman) substitution model . The Expected-Likelihood Weights (ELW) of 1,000 local rearrangements were used as confidence values of TreeFinder tree branches. Phylogenetic tree topologies were compared using the Approximately Unbiased (AU) test .
Relationships between viral families
At the first step, relationships between the 6 NCLDV families (Poxviridae, Asfarviridae, Irido- and Ascoviridae, Mimiviridae, Phycodnaviridae, and Marseillevirus) were resolved by analysis of the 49 NCVOGs that included representatives of at least 4 of the 6 families (49 clusters; Additional File 1). For these NCVOGs, ML trees were built from protein sequence alignments. Only 10 out of 49 NCVOGs produced alignments and trees deemed suitable for further analysis; the rest were discarded for one of the following reasons: there were too few (less than two) representatives from one or more families; there were too few (less than 100) conserved positions; one or more viral families appeared non-monophyletic. All 105 possible topologies corresponding to the relationships between 6 viral families were compared to the topologies of the 10 trees of individual conserved genes using the TOPD software . The consensus topology (Figure 7) was supported by 5 of the 10 NCVOGs (HelicaseII, A2L-like transcription factor (Pox_VLTF3), RNA polymerase A, RNA polymerase B, mRNA capping enzyme) and was accordingly chosen as the family-level consensus topology.
Relationships between species
At the second step, topologies inside Irido-, Phycodna-, and Poxviridae were resolved as follows. NCVOGs with high representation of family members (19 NCVOGs for Iridoviridae, 12 for Phycodnaviridae and 43 for Poxviridae) were used to build ML trees from protein sequence alignments. Two to four orthologs from other NCLDV families or cellular homologs were used as the outgroup for Iridoviridae and Phycodnaviridae; Poxviridae trees were rooted between Chordopoxvirinae and Entomopoxvirinae. After discarding poorly conserved families (less than 100 conserved positions) 17, 6 and 42 trees remained for Iridoviridae, Phycodnaviridae and Poxviridae, respectively. The topology most compatible with the rest of the family-specific trees was identified using the Bootsplit method  and used as the consensus.
Full consensus tree
The topologies obtained at the first and second steps were combined in a consensus tree. A concatenated alignment of four proteins present in all 45 species (D5_helicase_primase, DNApol_B, Pox_A32_pfam04665 and Pox_VLTF3) was used to calculate branch lengths and ELW values for the consensus tree using TreeFinder .
Neighbor-Joining gene content trees from phyletic patterns
Gene content trees for 15 NCLDV subfamilies were constructed as follows. Original 45 × 1445 binary presence/absence matrix (genome-level phyletic patterns) was converted into the 15 × 1445 subfamily-level presence/absence matrix by applying the logical OR operation within a subfamily (i.e. a subfamily registers a presence of an NCVOG if at least one genome of this subfamily has a protein from this NCVOG). For each pair of subfamilies the number of NCVOGs present in each of them (N1 and N2) as well as the number of NCVOGs present in both (NU) were computed. Then a gene content similarity measure (s) was calculated as either s = NU/min(N1, N2) or s = NU/sqrt(N1 × N2) and converted to a distance measure (d) as either d = 1-s or d = -ln(s). Neighbor-joining trees were constructed from the distance matrices using the NEIGHBOR program of Phylip 3.66 . Bootstrap values were obtained by 100 resamplings of the subfamily-level phyletic patterns.
Reconstruction of gene gain and loss events during the evolution of NCLDVs
Reconstruction of gene content evolution in the history of the NCLDV was performed using Count software http://www.iro.umontreal.ca/~csuros/gene_content/count.html[37, 53]. The software infers gene gain, loss and duplication rates on the branches of the species tree from the 45 × 1445 matrix of genome-level phyletic patterns using the likelihood maximization based on a phylogenetic birth-and-death model. The consensus tree (Figure 3) was used as the guide topology; the model assumed the Poisson family size distribution at the tree root and uniform gain, loss and duplication rates. Inferred model parameters include probabilities for each NCVOG to be present in each of the ancestral nodes. The sum of these probabilities gives a relatively robust estimate of the ancestral genome size, whereas the specific list of the ancestral NCVOGs is a subject to much uncertainty because it might include multiple low-confidence families. Here we chose to report high-confidence (p > 0.9) genes as the likely candidates for the ancestral gene set.
We thank Pere Puigbo Avalos (NCBI), Liran Carmel (Hebrew University) and Miklós Csûrös (Université de Montréal) for their help with phylogenetic analysis and ancestral genome reconstruction. The research of NY, YIW and EVK is supported by the DHHS Intramural Program (NIH, National Library of Medicine).
- Fields BN, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE, Knipe DM, (eds.): Fields Virology. New York: Lippincott Williams & Wilkins; 2001.Google Scholar
- Forterre P: The origin of viruses and their possible roles in major evolutionary transitions. Virus Res 2006,117(1):5-16. 10.1016/j.virusres.2006.01.010View ArticlePubMedGoogle Scholar
- Raoult D, Forterre P: Redefining viruses: lessons from Mimivirus. Nat Rev Microbiol 2008,6(4):315-319. 10.1038/nrmicro1858View ArticlePubMedGoogle Scholar
- Koonin EV, Senkevich TG, Dolja VV: The ancient Virus World and evolution of cells. Biol Direct 2006, 1: 29. 10.1186/1745-6150-1-29PubMed CentralView ArticlePubMedGoogle Scholar
- Iyer LM, Aravind L, Koonin EV: Common origin of four diverse families of large eukaryotic DNA viruses. J Virol 2001,75(23):11720-11734. 10.1128/JVI.75.23.11720-11734.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Iyer LM, Balaji S, Koonin EV, Aravind L: Evolutionary genomics of nucleo-cytoplasmic large DNA viruses. Virus Res 2006,117(1):156-184. 10.1016/j.virusres.2006.01.009View ArticlePubMedGoogle Scholar
- Van Etten JL: Unusual life style of giant chlorella viruses. Annu Rev Genet 2003, 37: 153-195. 10.1146/annurev.genet.37.110801.143915View ArticlePubMedGoogle Scholar
- Filee J: Lateral gene transfer, lineage-specific gene expansion and the evolution of Nucleo Cytoplasmic Large DNA viruses. J Invertebr Pathol 2009,101(3):169-171. 10.1016/j.jip.2009.03.010View ArticlePubMedGoogle Scholar
- La Scola B, Desnues C, Pagnier I, Robert C, Barrassi L, Fournous G, Merchat M, Suzan-Monti M, Forterre P, Koonin E, Raoult D: The virophage as a unique parasite of the giant mimivirus. Nature 2008,455(7209):100-104. 10.1038/nature07218View ArticlePubMedGoogle Scholar
- Raoult D, Audic S, Robert C, Abergel C, Renesto P, Ogata H, La Scola B, Suzan M, Claverie JM: The 1.2-megabase genome sequence of Mimivirus. Science 2004,306(5700):1344-1350. 10.1126/science.1101485View ArticlePubMedGoogle Scholar
- Claverie JM, Abergel C: Mimivirus and its Virophage. Annu Rev Genet 2009, 43: 49-66. 10.1146/annurev-genet-102108-134255View ArticlePubMedGoogle Scholar
- Claverie JM, Abergel C, Ogata H: Mimivirus. Curr Top Microbiol Immunol 2009, 328: 89-121. full_textPubMedGoogle Scholar
- Suzan-Monti M, La Scola B, Raoult D: Genomic and evolutionary aspects of Mimivirus. Virus Res 2006,117(1):145-155. 10.1016/j.virusres.2005.07.011View ArticlePubMedGoogle Scholar
- Claverie JM, Ogata H, Audic S, Abergel C, Suhre K, Fournier PE: Mimivirus and the emerging concept of "giant" virus. Virus Res 2006,117(1):133-144. 10.1016/j.virusres.2006.01.008View ArticlePubMedGoogle Scholar
- Koonin EV: Virology: Gulliver among the Lilliputians. Curr Biol 2005,15(5):R167-169. 10.1016/j.cub.2005.02.042View ArticlePubMedGoogle Scholar
- Filee J, Pouget N, Chandler M: Phylogenetic evidence for extensive lateral acquisition of cellular genes by Nucleocytoplasmic large DNA viruses. BMC Evol Biol 2008, 8: 320. 10.1186/1471-2148-8-320PubMed CentralView ArticlePubMedGoogle Scholar
- Moreira D, Brochier-Armanet C: Giant viruses, giant chimeras: the multiple evolutionary histories of Mimivirus genes. BMC Evol Biol 2008, 8: 12. 10.1186/1471-2148-8-12PubMed CentralView ArticlePubMedGoogle Scholar
- Filee J, Siguier P, Chandler M: I am what I eat and I eat what I am: acquisition of bacterial genes by giant viruses. Trends Genet 2007,23(1):10-15. 10.1016/j.tig.2006.11.002View ArticlePubMedGoogle Scholar
- Boyer M, Yutin N, Pagnier I, Barrassi L, Fournous G, Espinosa M, Robert C, Azza A, Sun S, Rossmann MG, Suzan-Monti M, La Scola B, Koonin EV, Raoult D: Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimaeric microorganisms. Proc Natl Acad Sci USA 2009, in press.Google Scholar
- Ghedin E, Claverie JM: Mimivirus relatives in the Sargasso sea. Virol J 2005, 2: 62. 10.1186/1743-422X-2-62PubMed CentralView ArticlePubMedGoogle Scholar
- Monier A, Claverie JM, Ogata H: Taxonomic distribution of large DNA viruses in the sea. Genome Biol 2008,9(7):R106. 10.1186/gb-2008-9-7-r106PubMed CentralView ArticlePubMedGoogle Scholar
- Monier A, Larsen JB, Sandaa RA, Bratbak G, Claverie JM, Ogata H: Marine mimivirus relatives are probably large algal viruses. Virol J 2008, 5: 12. 10.1186/1743-422X-5-12PubMed CentralView ArticlePubMedGoogle Scholar
- Kristensen DM, Mushegian AR, Dolja VV, Koonin EV: New dimensions of the virus world discovered through metagenomics. Trends Microbiol 2010, in press.Google Scholar
- Claverie JM, Grzela R, Lartigue A, Bernadac A, Nitsche S, Vacelet J, Ogata H, Abergel C: Mimivirus and Mimiviridae: giant viruses with an increasing number of potential hosts, including corals and sponges. J Invertebr Pathol 2009,101(3):172-180. 10.1016/j.jip.2009.03.011View ArticlePubMedGoogle Scholar
- Fitch WM: Distinguishing homologous from analogous proteins. Systematic Zoology 1970, 19: 99-106. 10.2307/2412448View ArticlePubMedGoogle Scholar
- Koonin EV: Orthologs, Paralogs and Evolutionary Genomics. Annu Rev Genet 2005, 39: 309-338. 10.1146/annurev.genet.39.073003.114725View ArticlePubMedGoogle Scholar
- Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, et al.: The COG database: an updated version includes eukaryotes. BMC Bioinformatics 2003, 4: 41. 10.1186/1471-2105-4-41PubMed CentralView ArticlePubMedGoogle Scholar
- Tatusov RL, Koonin EV, Lipman DJ: A genomic perspective on protein families. Science 1997,278(5338):631-637. 10.1126/science.278.5338.631View ArticlePubMedGoogle Scholar
- Mulkidjanian AY, Koonin EV, Makarova KS, Mekhedov SL, Sorokin A, Wolf YI, Dufresne A, Partensky F, Burd H, Kaznadzey D, Haselkorn R, Galperin MY: The cyanobacterial genome core and the origin of photosynthesis. Proc Natl Acad Sci USA 2006,103(35):13126-13131. 10.1073/pnas.0605709103PubMed CentralView ArticlePubMedGoogle Scholar
- Makarova KS, Sorokin AV, Novichkov PS, Wolf YI, Koonin EV: Clusters of orthologous genes for 41 archaeal genomes and implications for evolutionary genomics of archaea. Biol Direct 2007, 2: 33. 10.1186/1745-6150-2-33PubMed CentralView ArticlePubMedGoogle Scholar
- Koonin EV: Comparative genomics, minimal gene-sets and the last universal common ancestor. Nat Rev Microbiol 2003,1(2):127-136. 10.1038/nrmicro751View ArticlePubMedGoogle Scholar
- Charlebois RL, Doolittle WF: Computing prokaryotic gene ubiquity: rescuing the core from extinction. Genome Res 2004,14(12):2469-2477. 10.1101/gr.3024704PubMed CentralView ArticlePubMedGoogle Scholar
- Koonin EV, Mushegian AR, Bork P: Non-orthologous gene displacement. Trends Genet 1996,12(9):334-336. 10.1016/0168-9525(96)20010-1View ArticlePubMedGoogle Scholar
- Shimodaira H: An approximately unbiased test of phylogenetic tree selection. Syst Biol 2002,51(3):492-508. 10.1080/10635150290069913View ArticlePubMedGoogle Scholar
- Ogata H, Toyoda K, Tomaru Y, Nakayama N, Shirai Y, Claverie JM, Nagasaki K: Remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen African swine fever virus. Virol J 2009, 6: 178. 10.1186/1743-422X-6-178PubMed CentralView ArticlePubMedGoogle Scholar
- Wolf YI, Rogozin IB, Grishin NV, Koonin EV: Genome trees and the tree of life. Trends Genet 2002,18(9):472-479. 10.1016/S0168-9525(02)02744-0View ArticlePubMedGoogle Scholar
- Csuros M, Miklos I: Streamlining and large ancestral genomes in Archaea inferred with a phylogenetic birth-and-death model. Mol Biol Evol 2009,26(9):2087-2095. 10.1093/molbev/msp123PubMed CentralView ArticlePubMedGoogle Scholar
- Senkevich TG, Koonin EV, Bugert JJ, Darai G, Moss B: The genome of molluscum contagiosum virus: analysis and comparison with other poxviruses. Virology 1997,233(1):19-42. 10.1006/viro.1997.8607View ArticlePubMedGoogle Scholar
- Yutin N, Koonin EV: Evolution of DNA ligases of Nucleo-Cytoplasmic Large DNA viruses of eukaryotes: a case of hidden complexity. Biology Direct 2009,4(1):51. 10.1186/1745-6150-4-51PubMed CentralView ArticlePubMedGoogle Scholar
- Banerji S, Aurass P, Flieger A: The manifold phospholipases A of Legionella pneumophila - identification, export, regulation, and their link to bacterial virulence. Int J Med Microbiol 2008,298(3-4):169-181. 10.1016/j.ijmm.2007.11.004View ArticlePubMedGoogle Scholar
- Koonin EV: A duplicated catalytic motif in a new superfamily of phosphohydrolases and phospholipid synthases that includes poxvirus envelope proteins. Trends Biochem Sci 1996,21(7):242-243.View ArticlePubMedGoogle Scholar
- Husain M, Moss B: Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13L protein and phospholipase D. J Virol 2002,76(15):7777-7789. 10.1128/JVI.76.15.7777-7789.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Koonin EV, Wolf YI, Nagasaki K, Dolja VV: The Big Bang of picorna-like virus evolution antedates the radiation of eukaryotic supergroups. Nat Rev Microbiol 2008,6(12):925-939. 10.1038/nrmicro2030View ArticlePubMedGoogle Scholar
- Lukashin AV, Borodovsky M: GeneMark.hmm: new solutions for gene finding. Nucleic Acids Res 1998,26(4):1107-1115. 10.1093/nar/26.4.1107PubMed CentralView ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997,25(17):3389-3402. 10.1093/nar/25.17.3389PubMed CentralView ArticlePubMedGoogle Scholar
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004,32(5):1792-1797. 10.1093/nar/gkh340PubMed CentralView ArticlePubMedGoogle Scholar
- Marchler-Bauer A, Bryant SH: CD-Search: protein domain annotations on the fly. Nucleic Acids Res 2004, (32 Web Server):W327-331. 10.1093/nar/gkh454Google Scholar
- Jobb G, von Haeseler A, Strimmer K: TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. BMC Evol Biol 2004, 4: 18. 10.1186/1471-2148-4-18PubMed CentralView ArticlePubMedGoogle Scholar
- Whelan S, Goldman N: A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach. Mol Biol Evol 2001,18(5):691-699.View ArticlePubMedGoogle Scholar
- Puigbo P, Garcia-Vallve S, McInerney JO: TOPD/FMTS: a new software to compare phylogenetic trees. Bioinformatics 2007,23(12):1556-1558. 10.1093/bioinformatics/btm135View ArticlePubMedGoogle Scholar
- Puigbo P, Wolf YI, Koonin EV: Search for a 'Tree of Life' in the thicket of the phylogenetic forest. J Biol 2009,8(6):59. 10.1186/jbiol159PubMed CentralView ArticlePubMedGoogle Scholar
- Felsenstein J: Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods Enzymol 1996, 266: 418-427. full_textView ArticlePubMedGoogle Scholar
- Csuros M, Miklos I: A probabilistic model for gene content evolution with duplication, loss, and horizontal transfer. Lecture Notes in Computer Science 2006, 3909: 206-220. full_textView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.