Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
© Branco and Garry; licensee BioMed Central Ltd. 2009
Received: 10 September 2009
Accepted: 24 September 2009
Published: 24 September 2009
There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, detection of early markers of Lassa virus (LASV) infection may improve diagnosis and ultimately successful treatment with antivirals. Characterization of LASV GP1 ectodomain shedding is an important step toward developing sensitive diagnostics to detect circulating levels of this viral glycoprotein in infected patient sera.
Secretion of GP1 from mammalian cells expressing a native LASV GPC gene was not mediated by proteolytic cleavage, as determined by treatment with a panel of matrix metalloprotease (MMP) inhibitors. The shedding of GP1 was also not the result of over-expression of GPC under the control of a strong intron-A containing CMV promoter, as the soluble component could be immunoprecipitated from supernatants of cells expressing low levels of GPC under the control of an intronless promoter. Cells transfected with GPC retained surface membrane-associated expression of GP1 as determined by immunofluorescence assay, in addition to secreting the glycoprotein.
Secreted GP1 derived from GPC expression has a higher content of high mannose N-linked glycosylation than sGP1 expressed independently from the GP2 portion of the protein. Neither GP1 isoform contains sialylated N-glycans, O-linked carbohydrate chains, or galactose-β(1-4)-N-acetylglucosamine commonly present in complex and hybrid N-glycan structures.
These results demonstrate the non-proteolytic secretory nature of GP1 shedding during expression of the arenaviral glycoprotein complex. This phenomenon parallels shedding of a secretory glycoprotein component in filovirus replication. The glycosylation pattern of soluble GP1 resulting from expression of GPC was different from that of a soluble GP1 construct (sGP1-RRAA-FLAG), highlighting the intricately orchestrated post translational processing of the LASV glycoprotein complex.
Lassa virus, a member of the Arenaviridae family, is the etiologic agent of Lassa fever, which is an acute and often fatal illness endemic to West Africa. There are an estimated 300,000 - 500,000 cases of Lassa fever each year [1–3], with a mortality rate of 15%-20% for hospitalized patients and as high as 50% during epidemics [4, 5]. Presently, there is no licensed vaccine or immunotherapy available for preventing or treating this disease. Although the antiviral drug ribavirin is beneficial, it must be administered at an early stage of infection to successfully alter disease outcome, thereby limiting its utility . Furthermore, there is no commercially available Lassa fever diagnostic assay, thus preventing early detection and rapid implementation of existing treatment regimens (e.g. ribavirin administration). The lack of adequate countermeasures and means of detection, coupled with the severity of disease, contributed to the classification of LASV as a National Institutes of Allergy and Infectious Diseases (NIAID) Category A pathogen and biosafety level-4 (BSL-4) agent.
The LASV genome is comprised of two ambisense, single-stranded RNA molecules, designated small (S) and large (L) . Two genes on the S segment encode the nucleoprotein (NP) and two envelope glycoproteins (GP1 and GP2); whereas, the L segment encodes the viral polymerase (L protein) and RING finger Z matrix protein. GP1 and GP2 subunits result from post-translational cleavage of a precursor glycoprotein (GPC) by the protease SKI-1/S1P . GP1 serves a putative role in receptor binding, while GP2 has the structural features characteristic of class I viral fusion proteins .
Recently we reported that expression of wild type LASV GPC in mammalian cells results in the generation of significant levels of soluble GP1 in the supernatants of transfected cells, that is not associated with GP2 . GP1 ectodomain shedding from cells expressing wild type LASV GPC establishes potential new correlates of disease progression and highlights additional opportunities for development of diagnostics targeting the early stages of Lassa fever. In these studies the mechanism of LASV GP1 ectodomain shedding was further elucidated and its characteristics compared and contrasted to a similar phenomenon in filoviruses, as previously reported for Ebola virus (EBOV) .
Matrix metalloprotease inhibitors do not affect the secretion of GP1 from LASV GPC expressing cells
Secretory pathway inhibitors abrogate secretion of GP1 from LASV GPC expressing cells
Secretion of GP1 occurs in cells expressing high or low levels of LASV GPC
GP1 remains associated with the cell membrane in LASV GPC expressing cells
Secreted GP1 derived from expression of LASV GPC and sGP1 have disparate glycosylation patterns
Secreted GP1 from GPC and sGP1 expression have identical N-terminal sequences
Immunoprecipitated and PNGase-F deglycosylated GP1 protein from GPC and sGP1-RRAA-FLAG were subjected to SDS-PAGE and transferred to PVDF membranes for N-terminal sequence analysis by Edman degradation. The six N-terminal amino acids from each protein were T-S-L-Y-K-G, thus representing the SPase-cleaved form of the LASV GP1 protein, as previously characterized .
Effects of matrix metalloprotease and secretory pathway inhibitors on GP1 shedding and cytotoxicty in HEK-293T/17 cells
 in assay
effect on GP1 shedding
1 - 10 μM working 
IC50 = 4.6 μM
MMP-9 inhibitor I
IC50 = 5 nM
MMP-2 inhibitor I
Ki = 1.7 μM
Ki = 400 pM - 27 nM
IC50 = 100 nM
1 - 10 mM working 
1 - 10 mM working 
BD Golgi Plug™
BD Golgi Stop™
The mammalian expression system employed in these studies was originally developed for high level production of recombinant LASV proteins for diagnostic assay development . High level expression of soluble LASV GPC resulted in both secretion of glycoprotein precursor uncleaved by SKI-1/S1P, and high levels of sGP1. Although CMV promoter strength has not been compared to that of the LASV promoter, it is reasonable to suggest that very high level expression of recombinant LASV genes in mammalian cells could result in artefactual processing, secretion, and stoichiometry of glycoprotein complex components. It is evident that high level expression of soluble GPC in HEK-293T/17 cells driven by CMV plus intron-A overwhelms cellular SKI-1/S1P protease, as approximately one half of the secreted glycoprotein is uncleaved . In order to address the possibility that overexpression of GPC resulted in artefactual secretion of GP1 we expressed the glycoprotein from an intronless CMV-driven promoter. Expression of GPC from CMV-intron-A driven vectors resulted in 8 - 10 fold higher levels of cleaved intracellular GP1 than from intronless counterparts. Low levels of sGP1 in the supernatants of cells expressing intronless GPC were detected only when the glycoprotein was immunoprecipitated. An 8 - 10 fold dilution of culture supernatant from cells expressing sGP1 still resulted in detection of significant levels of secreted glycoprotein, whereas similar levels of immunoprecipitated GP1 from intronless GPC expression required 400 fold higher volume of supernatant. These results suggest that overexpression of GPC results in disproportionately enhanced secretion of sGP1 when compared to lower expression levels of the glycoprotein. Generally, expression and secretion levels of GP1 in the HEK-293T/17 line paralleled those obtained with VERO cells, a common line used for the propagation of Lassa virions and analysis of LASV gene expression (Figure 3D).
Association of GP1 with the cell membrane as a component of the tripartite glycoprotein complex was verified by staining live cells overexpressing GPC. After extensive cell washing to remove sGP1, followed by staining with a LASV GP1-specific mAb, cell surface associated GP1 could be readily detected. The staining pattern was less diffuse than with fixed and permeabilized cells overexpressing GPC, with bright fluorescence primarily associated with the cell membrane. Thus, a fraction of the GP1 protein associates with GP2 and SSP on the cell membrane of GPC-transfected cells, whereas a soluble, un-associated component is shed into the cellular milieu.
Sequencing of GP1 protein from expression of GPC or sGP1 revealed identical N-termini through six amino acids, suggesting that both forms of the glycoprotein result from cleavage of pre-GPC by SPase at amino acid 58, and that the SSP functions as the secretory signal.
Protein deglycosylation and lectin binding studies revealed significant heterogeneity in the carbohydrate composition of the LASV GP1 protein. Whereas secreted GP1 from expression of LASV GPC could be fully deglycosylated with PNGase F (Figure 5A, lane 2), two distinct forms could be observed in sGP1 from sGP1-RRAA-FLAG expression. The 22 KDa band corresponding to a fully deglycosylated sGP1 migrated just below a ca. 24 KDa form of the protein, and it was consistently detected on western blots (Figure 5B, lane 2). The average 14-residue precursor of N-linked oligosaccharides adds approximately 2.6 KDa to the mass of a target protein ([GlnNAc × 3] + [Man × 9] + [Glc × 3]). Thus, the 24 KDa form of sGP1-RRAA-FLAG that remains after digestion with PNGase F suggests the presence of an oligosaccharide branch other than N-linked in nature. Alternately, as PNGase F is not able to cleave N-linked glycans from glycoproteins when the innermost GlcNAc residue is linked to an α1-3 Fucose residue , the possibility of this form of linkage in sGP1-RRAA-FLAG exists. This type of modification is most commonly found in plant and some insect glycoproteins, however. Computational analysis of the LASV GP1 protein sequence using OGPET v1.0 (University of Texas, El Paso  revealed the possibility of an O-GalNAc (mucin type) glycosylation in GP1 at Ser 80 (SnIS TnHL), with a predictive score of 0.599833 (cutoff = 0.4247). Additionally, NetGlycate 1.0 Server , a predictor of glycation of ε amino groups of lysines in mammalian glycoproteins, attributed a score of 0.914 to Lys5 in LASV GP1, which is significantly above the threshold of 0.5. Lectin-binding assays did not aid in resolving the glycosylation pattern of the 24 KDa form of sGP1-RRAA-FLAG. The lectins PNA (Peanut agglutinin) and DSA (Datura stramonium agglutinin), which recognize the core disaccharide galactose (1-3) N-acetylgalactosamine, and Gal-(1-4)GlcNAc in complex and hybrid glycans, in O-glycans, and GlnNAc in O-glycans, respectively, did not bind to the 24 KDa sGP1 form. A higher degree of N-linked glycosylation heterogeneity on LASV GP1 was obtained in Endo H digestion profiles. Endo H removes only high mannose and some hybrid types of N-linked carbohydrates from glycoproteins, and will not cleave more complex glycan structures [19, 20]. Soluble GP1 from GPC expression revealed a more heterogeneous distribution of high mannose and hybrid glycosylation than were present in sGP1-RRAA-FLAG, indicating a higher content of mannose and/or hybrid structures. Conversely, it appears that sGP1-RRAA-FLAG has a significantly higher content of complex glycan structures, as determined by reduced EndoH activity on the glycoprotein. These results were confirmed by the differential staining of both GP1 forms by GNA; soluble GP1 from GPC expression was heavily stained with GNA, whereas sGP1-RRAA-FLAG showed significantly dimmer reactivity. Densitometry analysis showed a ratio of 2.27 ± 0.014 in GNA staining intensity of GPC-derived GP1 versus sGP1-RRAA-FLAG (N = 3). The marked differences in glycosylation of each form of LASV GP1 could be attributed to a potentially different translational and post-translational modification profile and timeline in expressing cells. The process of translation, ER translocation, SPase and SKI-1/S1P cleavage, ER retention of glycoproteins, and assembly of the LASV tripartite complex (GP1+GP2+SSP) has been somewhat dissected, and constitutes a finely orchestrated process in the ER and Golgi of mammalian cells. The correct processing and assembly of the tripartite complex is essential for subsequent binding of LASV virions via GP1 to the α-dystroglycan cellular receptor, and initiation of viral entry via the fusion peptide on the N-terminus of GP2, a process largely mediated by the presence of the intact SSP peptide. Thus, it is conceivable that LASV GPC-derived glycoproteins remain accessible to ER resident glucosyltransferases, and the homologous lectins calnexin and calreticulin longer than sGP1-RRAA-FLAG, resulting in an overall different endpoint glycosylation pattern. Glucosyltransferases act as one of the primary protein folding quality control mechanisms in the ER, in concert with lectins, peptidyl-propyl-isomerases, and chaperones that are tightly regulated by nuclear transcription mediated by ER membrane resident proteins (e.g. ATF6) that sense high levels of protein misfolding in this organelle . The formation of hybrid and complex glycans takes place in the medial and trans Golgi compartments. Therefore, proteins residing in the ER which have not passed through the Golgi cannot possess hybrid or complex N-linked structures. Thus, the intricate assembly process for the LASV GPC complex would be expected to differ from that of single glycoprotein expression from the sGP1-RRAA-FLAG construct. The kinetics of GP1 secretion from expression of GPC and sGP1-RRAA-FLAG do not differ significantly, as the onset for detection of each form of the glycoprotein in the extracellular medium occurs at approximately 12 hours post transfection (with CMV+intronA driven constructs). Post-translational processing and secretion of sGP1-RRAA-FLAG is not necessarily faster than GPC expressed GP1, but it is executed differently. It is noteworthy to point that such findings contrast with previously reported results by our group that showed clear and complete removal of high mannose glycans from sGP1 and sGP2 generated with the same expression constructs and cell type . Such contrasting results point to subtle differences in culture conditions that could significantly affect resulting glycosylation patterns. Although the HEK-293T/17 clone used in both studies was obtained from the same source (American Type Culture Collection [ATCC]), the studies were performed with different lots of culture media and supplements, namely fetal bovine serum. It has been extensively reported that subtle differences in cell culture environments, including changes in medium composition can result in significant differences in glycosylation patterns of secreted glycoproteins. Such impact on glycosylation is a primary focus of product quality control in many glycoprotein-based biopharmaceutical products, such as therapeutic antibodies, erythropoietin, and reproductive hormones [22–26]. Lastly, treatment of secreted GP1 from GPC and sGP1-RRAA-FLAG expression did not result in observable shifts with neuraminidase. Neuraminidase (Acetyl-neuraminyl hydrolase; Sialidase) catalyzes the hydrolysis of α2-3, α2-6, and α2-8 linked N-acetyl-neuraminic acid residues from glycoproteins and oligosaccharides [27, 28]. These results were further confirmed by the lack of binding to both forms of GP1 by the lectins MAA (Maackia amurensis agglutinin) and SNA (Sambucus nigra agglutinin), which are specific for α2-3 and α2-6 galactose, respectively. Thus, it appears that LASV GP1 is not synthesized with terminal sialic acid residues in its carbohydrate profile, irrespective of the glycoprotein precursor.
It is well known that hydrophobic interactions predominate over the proper and improper folding of proteins. Further, the hydrophobic areas of high mannose N-glycans are closely associated with promotive effects on protein folding, possibly depressing the hydrophobic interactions unfavorable to the proper folding of nascent polypeptides in the E.R. It has been established by Jitsuhara et al. , Narhi et al. , and others that higher oligomannose chains of glycosylated proteins promote protein folding more strongly than lower ones . Of noteworthy mention is the folding of influenza virus hemagglutinin with a truncated N-glycan Glc1Man5GlcNAc2 moiety that normally interacts with the lectin calnexin in the E.R. is markedly depressed . The E.R. resident lectins calnexin and calreticulin tether monoglucosylated high mannose N-glycans from nascent proteins, resulting in suppression of aggregation, thus promoting protein folding . Many studies have established the correlation between proper protein glycosylation with the acquisition and retention of glycoprotein biological activities. It is reasonable to expect that in the arenaviral tripartite complex the proper folding of the individual glycoprotein subunits, and the interaction with the SSP, have critical roles in establishing functional binding of virions to host cell receptors and subsequent fusion and viral entry. Our own previous LASV GP1 protein expression studies in multiple biological systems have resulted in markedly different results. Although expression of full length GP1 in E. coli was achievable, the lack of glycosylation in the prokaryotic system required the presence of sodium dodecyl sulfate (SDS), reducing agents (e.g. dithiothreitol [DTT]), and the zwitterion morpholino-ethanesulfonic acid (MES) in the purification scheme in order to yield non-aggregated protein . Further processing of bacterial expressed GP1 protein for analytical assays that required removal of SDS and DTT readily resulted in aggregation. In contrast, expression of multiple forms of LASV GP1 in mammalian cells always resulted in glycosylated, soluble protein, although aggregation of the standalone glycoprotein remained an issue . Further characterization of post-translational processing of the arenaviral glycoprotein complex may be of crucial importance in the establishment of viral infectivity correlates, as well as pave the way for identification of disease susceptibility markers, and development of effective vaccines and therapeutics.
Together, these data strongly suggest dual modalities in LASV GPC expression; the formation of a fully functional tripartite complex for display on the surface of infectious virions, and the secretion of a soluble GP1 component with as of yet uncharacterized function. The fate of un-associated GP2 during expression of LASV GPC was not investigated in these studies, but it warrants analysis, as the stoichiometry of the glycoprotein complex depends on the ratio of the available individual components. Dolnik et al.  have partly characterized the role of a soluble GP component in EBOV, and have proposed a role in the infectivity of the virus in vivo. According to Dolnik et al., the secreted EBOV GP component acts as an immune decoy, by targeting the humoral immune system and possibly removing from circulation antibodies with innate neutralizing activity against the virus. This mechanism would provide circulating infectious virions a clearer path toward infection of new target cells, with enhanced virulence. Also, Wahl-Jensen et al. have further delineated the role of secreted EBOV GP in activation of primary target cells [35, 36]. These studies revealed that rigid presentation of the EBOV glycoprotein, as it's displayed on the surface of virions is critical for activation of pro-inflammatory cytokines and chemokines, whereas soluble glycoprotein failed to elicit any response. Additionally, EBOV GP(1,2) in viral particle-associated form mediated endothelial cell activation and decreased cell barrier function, whereas sGP, the major soluble glycoprotein of EBOV, demonstrated an anti-inflammatory role by protecting the endothelial cell barrier function.
Although a similar mechanism has not been ascribed to arenaviruses, our work presents a platform upon which to investigate this phenomenon in vivo. The secreted GP1 component resulting from GPC expression has not been detected in the serum of LASV infected patients, and further work will be performed by our group in an attempt to test the feasibility of this hypothesis. If the phenomenon of GP1 shedding is present in vivo the glycoprotein should be preferentially detected in the early stages of arenaviral infection, when circulating antigens are present at the highest levels. Soluble GP1 in serum could be separated from virions by selective size filtration, thus providing a simple and easily deployable platform for identification of the soluble glycoprotein component. If confirmed, arenaviral glycoprotein shedding could result in the development of more sensitive and earlier detection diagnostics, as well as establish new correlates of pathogenesis and immune evasion by this class of hemorrhagic fever viruses.
The shedding of GP1 from cells expressing GPC results from secretion of a cleaved and un-associated fraction of the protein. This phenomenon is not the result of cleavage of GP1 from the tripartite complex expressed on the cell surface, and it is not mediated by cellular MMPs. Instead, secretion of GP1 occurs when GPC is processed into GP1 and GP2 in the ER, and a significant fraction of this protein is shuttled through the secretory pathway and is released into the extracellular medium. Both forms of secreted GP1 share N-terminal sequences, but have dissimilar glycosylation profiles that suggest a differential retention and glycoprotein processing timeline in the ER and Golgi compartments.
Cells, plasmids, antibodies
HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS).
Vero cells (ATCC CRL 1587) were maintained in cDMEM supplemented with NEAA and 5% ΔFBS. Plasmid constructs expressing LASV GPC, GP1-TM, and sGP1, and the backbone vector pcDNA3.1+zeo:intA were described elsewhere . For immunoassays, Dr. Randal Schoepp kindly provided the LASV-specific GP1 mAb L52-74-7A, which was generated against purified gamma-irradiated LASV, as previously described . Horseradish peroxidase (HRP)-conjugated secondary antibodies specific for mouse IgG were purchased from Kirkegaard and Perry Laboratories (KPL, Gaithersburg, MD). Alexa Fluor 488-conjugated goat F(ab')2 anti-mouse IgG secondary antibodies for immunofluorescence assays were purchased from Molecular Probes. Anti-LASV GP1 mAb L52-74-7A was biotinylated with EZ-Link® NHS-Biotin (Pierce, Rockford, IL), and desalted with Zeba™ Desalt Spin Columns (Thermo Scientific, Rockford, IL), according to manufacturer's instructions.
Transient expression of LASV gene constructs
Recombinant LASV protein expression was analyzed in HEK-293T/17 or VERO cells transiently transfected with mammalian expression vector DNAs, which were prepared using the PureLink HiPure plasmid filter midiprep kit (Invitrogen, Carlsbad, CA). The negative control vector pcDNA3.1(+):intA was included in all transfections. Briefly, 1 × 106 cells were seeded per well of a Poly-D-Lysine-coated 6-well plate in 2 mL of cDMEM. After overnight incubation in growth conditions cells were transfected with unrestricted recombinant plasmid DNAs using Lipofectamine™ 2000 (Invitrogen), according to the manufacturer's instructions. Four μg of each plasmid DNA were used per transfection. Transfections were incubated for the times described in results, at 37°C, 5% CO2, 90% RH, after which cell culture supernatants were collected and clarified by centrifugation. To prepare cell extracts from transfected cultures, cell monolayers were carefully washed twice with Ca++- and Mg++-free PBS, pH7.4, collected by gentle dislodging, transferred to 1.5 mL polypropylene tubes, and lysed for 10 minutes in a mammalian cell lysis buffer comprised of 50 mM Tris buffer, pH 7.5, 1 mM EDTA, 0.1% SDS, 0.5% deoxycholic acid, 1% Igepal CA-360, and a protease inhibitor cocktail (Sigma Aldrich, St. Louis, MO), according to the manufacturer's instructions. The insoluble fraction was pelleted by centrifugation at 14,000 × g for 10 minutes, and the supernatants were transferred to fresh tubes. Protein concentration was determined for each sample by A280 with A260 subtraction, and verified using a Micro BCA™ Protein Assay Kit, as outlined by the manufacturer (Thermo Scientific).
Metalloprotease and secretory pathway inhibition assays
Metalloprotease inhibitors were purchased from Sigma (1,10-Phenanthroline monohydrate, 2,2'-Dipyridyl ReagentPlus®, Bestatin hydrochloride, Phosphoramidon disodium salt, α1-Antichymotrypsin, EDTA, EGTA) or Calbiochem (MMP-2 Inhibitor I, MMP-9 Inhibitor I, InSolution™ GM 6001, InSolution™ TAPI-1, Captopril), and resuspended in 100% DMSO (Sigma), or with cell culture medium when appropriate, at 100× concentration. Inhibitors were diluted in growth medium for analysis, at a final DMSO concentration of 1%. For inhibition assays HEK-293T/17 cells were transiently transfected with plasmid DNAs in 6-well plates, as described above, for 12 hours prior to the addition of metalloprotease inhibitors. Transfection medium was then removed and cells were washed twice with 1× PBS, pH7.4, followed by addition of 2 mL of fresh growth medium containing inhibitors. Plates were returned to the growth chamber and reactions were harvested between 42 - 45 hours post transfection by collecting supernatants and generating whole cell protein extracts, as described above. The Golgi secretory pathway inhibitors brefeldin A and monensin were purchased from Sigma, and diluted in growth medium to 15 μg/mL and 10 μM, respectively. Inhibitors were added to cells at the time of transfection with pcDNA3.1(+):intA or pcDNA3.1(+):intA:LASV GPC, or at 12 hours. Supernatants and cell extracts were harvested at 36 hours post transfection for western blot analysis.
Western blot and densitometry analyses
Expression of LASV GP1 in cell extracts and culture supernatants was confirmed by Western blot analysis using anti-LASV GP1 mAb L52-74-7A and a horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) secondary antibody. Briefly, 10 μg of total cell protein in 10 μL (~1 × 105 cell equivalents), or 26 μL of cell culture supernatant were resolved on 10% NuPAGE Novex Bis-Tris gels, according to the manufacturer's specifications (Novex, San Diego, CA). All samples in these studies were denatured and reduced in SDS-PAGE buffer containing DTT. Proteins were transferred to 0.45-μm nitrocellulose membranes, blocked, and probed in 1× PBS, pH 7.4, 5% non-fat dry milk, 0.05% Tween-20, and 0.1% thymerosal. Membranes were then incubated in LumiGlo chemiluminescent substrate (KPL) and exposed to Kodak BioMax MS Film. Developed films were subjected to high resolution scanning for densitometry analysis. Quantification of band intensity was performed using National Institutes of Health ImageJ 1.41o software http://rsb.info.nih.gov/ij, and following the procedure outlined in http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html, using TIFF files.
Soluble GP1 was immunoprecipitated from 1 mL of transfected culture supernatants with 2 μg of anti-LASV GP1 mAb L52-74-7A and Protein G-Sepharose® 4B Fast Flow, using a Protein G Immunoprecipitation Kit (Sigma), according to the manufacturer's instructions. Immunoprecipitated proteins were denatured and reduced, separated by SDS-PAGE, and blotted as outlined above. GP1 was detected with biotinylated anti-LASV GP1 mAb L52-74-7A and ImmunoPure® Streptavidin-HRP (Thermo Scientific).
Cell proliferation assay
HEK-293T/17 cell cytotoxicity induced by metalloprotease inhibitors was monitored with a TACS™ MTT Cell Proliferation Assay (R&D Systems, Minneapolis, MN), according to manufacturer's instructions. The transfection procedure outlined above for 6-well plates was scaled down to a 96-well format, with each condition analyzed in triplicate. Data was plotted as mean absorbance at 570 nm, with standard deviation, and background correction at 650 nm.
HEK-293T/17 cells were seeded on Poly-D-Lysine-coated sterile glass cover slips (BD Biosciences, Walkersville, MD) in 24-well plates. Cells were transfected as outlined above. At 72 hours post-transfection cells were washed with cold HBSS and were either fixed in 2% methanol-free formaldehyde, or incubated in 1× PBS, pH 7.4/1% heat inactivated FBS/0.1% NaN3 for live cell staining. Fixed cells were incubated for 20 minutes in 50 mM NH4Cl to reduce background fluorescence, followed by permeabilization with 0.1% Triton-X 100 for 5 minutes, and blocking with 8% heat inactivated goat serum for one hour. Cells were stained with anti-LASV GP1 mAb L52-74-7A diluted to 2 μg/mL in 0.8% goat serum/0.1% Triton-X 100, for one hour. After washing, cells were incubated with Alexa Fluor 488 Goat F(ab')2 anti-mouse IgG (Molecular Probes/Invitrogen) diluted to 1 μg/mL in staining buffer containing 1 μM DAPI (Invitrogen), for 1 hour. Cover slips were mounted on ethanol washed glass slides with ProLong® antifade reagent (Invitrogen). Reactions were cured for 48 hours prior to microscopy. Live cells were stained with the same primary, secondary antibodies and DAPI, for one hour each in 1× PBS, pH 7.4/1% heat inactivated FBS/0.1% NaN3, followed by mounting and curing, as described above. All reactions were performed at room temperature, and were washed three times with 1× DPBS between steps. Stained cells were imaged with a Zeiss Axioplan 2 fluorescent microscope equipped with an Intelligent Imaging Innovations CCD (Denver, CO), using a Plan-APOCHROMAT 63X/1,4 oil DIC objective.
PNGase F, Endo H, and Neuraminidase assays
The glycosylation patterns in secreted GP1 generated from expression of LASV GPC and sGP1 were resolved by treatment with the glycosidases PNGase F, Endo H, and Neuraminidase. Immunoprecipitated GP1 from transiently transfected HEK-293T/17 cell supernatants were subjected to treatment with 500 NEB U of PNGase F, Endo H, or Neuraminidase for 1 hour using the reaction conditions suggested by the manufacturer (New England Biolabs). Control reactions were similarly processed except that enzymes were not added. Following incubation proteins were resolved by reducing SDS-PAGE, blotted, probed with biotinylated anti-LASV GP1 mAb L52-74-7A and Streptavidin-HRP, and developed as described above.
Lectin-based Glycan differentiation assays
Glycosylation patterns on secreted GP1 were further characterized via binding of glycan-specific lectins using a DIG Glycan Differentiation Kit (Roche Applied Science, Mannheim, Germany), according to the manufacturer's instructions. Immunoprecipitated GP1 from expression of LASV GPC and sGP1 was resolved on SDS-PAGE, blotted onto nitrocellulose, and subjected to lectin binding assays.
N-terminal protein sequencing
Immunoprecipitated GP1 protein was resolved by SDS-PAGE, blotted onto PVDF membranes, stained with Ponceau Blue and destained in water. N-terminal sequence determination of the 42 KDa protein was performed by Edman degradation and carried out in an Applied Biosystems Model 4949 CLC protein suquenator. Phenylthiohydantoin derivatives of amino acids were analyzed on-line with an Applied Biosystems Model 785A/140C/610A analyzer. All reagents and solvents were from Applied Biosystems.
Statistical analysis of data was performed with GraphPad InStat, V3.06 (GraphPad Software, Inc., San Diego, CA), using Analysis of Variance (ANOVA).
This work was supported by Department of Health and Human Services/National Institutes of Health/National Institute of Allergy and Infectious Diseases Challenge and Partnership Grant Number 1 UC1 AI067188-01. The authors thank Dr. Kerstin Honer Zu Bentrup, Research Assistant Professor, Manager, Microbiology Imaging Facility, Tulane University Medical School Microbiology and Immunology Departments, for assistance with immunofluorescence microscopy and staining protocols. The authors also thank the Lassa Fever Diagnostic Development Consortium members Corgenix, Inc., Broomfield, CO; Vybion, Inc., Ithaca, NY; Lassa Fever Laboratory - Kenema Government Hospital, Kenema, Sierra Leone, for the supply of critical reagents and helpful discussions of results.
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