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Table 1 Effects of matrix metalloprotease and secretory pathway inhibitors on GP1 shedding and cytotoxicty in HEK-293T/17 cells

From: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms

inhibitor [] in assay Notes/references effect on GP1 shedding cytotox.(MTT) morphology (microscopy)
2,2 dipyridyl 1 μM Calbiochem - - enlarged cells
1,10 phenanthroline 10 μM ref. [12] - - detached cells
bestatin 10 μM 1 - 10 μM working [] - - enlarged cells
α1-antichymotrypsin 4 μM ref. [12] - - normal
phosphoramidon 10 μM IC50 = 4.6 μM - - enlarged cells
captopril 10 μM ref. [12] - - normal
MMP-9 inhibitor I 50 nM IC50 = 5 nM - - detached cells
MMP-2 inhibitor I 100 μM Ki = 1.7 μM - + enlarged cells
GM6001 5 μM Ki = 400 pM - 27 nM - - normal
TAPI-1 50 μM IC50 = 100 nM - - normal
EDTA 5 mM 1 - 10 mM working [] - - rounded cells
EGTA 5 mM 1 - 10 mM working [] - - rounded cells
brefeldin A 15 μg/mL BD Golgi Plug™ +++ + rounded cells
monensin 10 μM BD Golgi Stop™ +++ + rounded cells