Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms

Table 1 Effects of matrix metalloprotease and secretory pathway inhibitors on GP1 shedding and cytotoxicty in HEK-293T/17 cells

inhibitor	[] in assay	Notes/references	effect on GP1 shedding	cytotox.(MTT)	morphology (microscopy)
2,2 dipyridyl	1 μM	Calbiochem	-	-	enlarged cells
1,10 phenanthroline	10 μM	ref. [12]	-	-	detached cells
bestatin	10 μM	1 - 10 μM working []	-	-	enlarged cells
α1-antichymotrypsin	4 μM	ref. [12]	-	-	normal
phosphoramidon	10 μM	IC₅₀ = 4.6 μM	-	-	enlarged cells
captopril	10 μM	ref. [12]	-	-	normal
MMP-9 inhibitor I	50 nM	IC₅₀ = 5 nM	-	-	detached cells
MMP-2 inhibitor I	100 μM	K_i = 1.7 μM	-	+	enlarged cells
GM6001	5 μM	K_i = 400 pM - 27 nM	-	-	normal
TAPI-1	50 μM	IC₅₀ = 100 nM	-	-	normal
EDTA	5 mM	1 - 10 mM working []	-	-	rounded cells
EGTA	5 mM	1 - 10 mM working []	-	-	rounded cells
brefeldin A	15 μg/mL	BD Golgi Plug™	+++	+	rounded cells
monensin	10 μM	BD Golgi Stop™	+++	+	rounded cells

ISSN: 1743-422X