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Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection
Virology Journal volume 6, Article number: 103 (2009)
In order to elucidate how Hepatitis C Virus (HCV) interacts with polarized hepatocytes in vivo and how HCV-induced alterations in cellular function contribute to HCV-associated liver disease, a more physiologically relevant hepatocyte culture model is needed. As such, NASA-engineered three-dimensional (3-D) rotating wall vessel (RWV) bioreactors were used in effort to promote differentiation of HCV-permissive Huh7 hepatoma cells.
When cultured in the RWV, Huh7 cells became morphologically and transcriptionally distinct from more standard Huh7 two-dimensional (2-D) monolayers. Specifically, RWV-cultured Huh7 cells formed complex, multilayered 3-D aggregates in which Phase I and Phase II xenobiotic drug metabolism genes, as well as hepatocyte-specific transcripts (HNF4α, Albumin, TTR and α1AT), were upregulated compared to 2-D cultured Huh7 cells. Immunofluorescence analysis revealed that these HCV-permissive 3-D cultured Huh7 cells were more polarized than their 2D counterparts with the expression of HCV receptors, cell adhesion and tight junction markers (CD81, scavenger receptor class B member 1, claudin-1, occludin, ZO-1, β-Catenin and E-Cadherin) significantly increased and exhibiting apical, lateral and/or basolateral localization.
These findings show that when cultured in 3-D, Huh7 cells acquire a more differentiated hepatocyte-like phenotype. Importantly, we show that these 3D cultures are highly permissive for HCV infection, thus providing an opportunity to study HCV entry and the effects of HCV infection on host cell function in a more physiologically relevant cell culture system.
Hepatitis C virus (HCV), a liver tropic positive-stranded RNA flavivirus, infects ~170 million people worldwide, causing acute and chronic hepatitis and hepatocellular carcinoma . However, since its discovery in 1989, a major obstacle impeding HCV research has been the lack of robust cell culture and small animal infection models. Notably significant advancement has been made with the identification of a genotype 2a HCV consensus clone (Japanese Fulminant Hepatitis, JFH-1) that can replicate and produce infectious HCV in vitro in the Huh7 human hepatoma-derived cell line [2–4], allowing for the study of the entire viral life cycle. This system, however, is limited in that it makes use of a non-differentiated cell line that does not recapitulate the cellular conditions encountered by HCV in vivo [5, 6]. In particular, hepatocyte polarity is likely relevant to HCV entry as growing evidence suggests interplay between HCV and tight junction (TJ) proteins claudin-1 (CLDN1)  and occludin [8, 9] is essential for viral uptake. In fact, recent reports surprisingly suggests that hepatocyte polarity may restricts HCV entry [10, 11]. While an inverse relationship between viral entry and hepatocyte polarity would potentially represent a unique determinant of HCV entry, to date attempts to dissect this relationship have been difficult and inconclusive due to the inability of cell culture grown hepatocyte-derived cell lines, such as Huh7 cells, to mimic the complex polarized phenotype of hepatocytes in vivo. To circumvent these restriction, studies investigating HCV entry into Caco-2 cells  and HepG2 cells  have been performed as these cells can polarize to differing degrees in vitro, however, neither Caco-2 or HepG2 cells supports efficient HCV infection limiting their utility. As such, a more physiologically relevant hepatocyte tissue culture model is still needed to assess if cell polarity negatively affects HCV infection.
The NASA-engineered RWV is a horizontally rotating cylindrical culture vessel which reduces shear and turbulence associated with conventional stirred bioreactors; therefore, it simulates aspects of microgravity similar to the environment encountered during fetal development [12–14]. In contrast to conventional static tissue culture systems, cells grown in the RWV are cultured in "suspended animation" where they are continuously free-falling [12, 15]. Thus, while the 2-D environment of plastic substrates may alter gene expression and prevent cellular differentiation [12, 16–21], the fluid dynamics of the RWV culture system allow cells to co-localize into three-dimensional (3-D) aggregates, promoting in vivo-like exchange of growth factors and efficient cell-to-cell interactions [12–14, 20, 21]. This in vivo-like environment thus can promote transformed and primary cell lines to become more structurally and functionally similar to their in vivo counterparts [13, 15, 20–24].
In the current study we demonstrate that RWV-cultured Huh7 cells formed complex, multilayered, 3-D aggregates that exhibited up-regulation of metabolic and hepatocyte-specific transcripts as well as increased expression and re-localization of tight junction, cell adhesion, and polarity markers. Importantly, these aggregates remained highly permissive for HCV infection suggesting that hepatic polarity does not limit HCV entry in 3-D-cultured Huh7 cells. As such, RWV-cultured Huh7 cells may represent a more appropriate physiologically relevant system for further in vitro studies of HCV entry and infection dynamics.
Cell culture and viruses
Huh7 cells (also known as Huh7/scr cells [25, 26] and Huh7-1 cells ) were obtained from Dr. Chisari (The Scripps Research Institute, La Jolla, CA)  and cultured as previously described . 3-D Huh7 cultures were established using previously described techniques [13, 14], with minor modifications. Briefly, 5 × 106 Huh7 cells were trypisinized, incubated with 250 mg Cytodex-3 microcarrier beads (Sigma, St. Louis, MO) for 30 minutes at room temperature in a total volume of 30 ml complete DMEM. Cell-bead complexes were introduced into the RWV bioreactor (Synthecon, Inc., Houston, TX) at a ratio of 20 cells/bead, transferred to 37°C, and vessel rotation was initiated at 20 rotations per minute. Medium was replenished every 24 h and rotation speed was increased as aggregates developed to maintain cells in free-falling suspension.
Protocols for JFH-1 in vitro transcription and HCV RNA electroporation have been described elsewhere . JFH-1 cell culture-propagated HCV (HCVcc) viral stocks were obtained by infection of naïve Huh7-1 cells at a multiplicity of infection (MOI) of 0.01 focus forming units (FFU)/cell, using medium collected from Huh7 cells on day 18 post transfection with in vitro transcribed pJFH-1 RNA as previously described .
RNA isolation and RTqPCR
Total cellular RNA was isolated by the guanidine thiocyanate method using standard protocols . One μg of RNA was used for cDNA synthesis using TaqMan reverse transcription reagents (Applied Biosystems, Foster City, CA), followed by SYBR green real-time quantitative PCR analysis (RTqPCR) using an Applied Biosystems 7300 real-time thermocycler as previously described . Relative expression levels of hepatocyte-specific genes and Phase I and Phase II metabolic genes were assessed using the primers described in  and normalized to β-actin levels. HCV JFH-1 and GAPDH transcript levels were determined relative to a standard curve of serially diluted plasmid containing the JFH-1 cDNA or the human GAPDH gene, respectively, using primers described in .
Immunofluorescence analysis of aggregates was performed as previously described . Briefly, Huh7 3-D aggregates were fixed with 4% (v/v) paraformaldehyde (Sigma, St. Louis, MO), free aldehydes quenched with 50 mM NH4Cl in DPBS for 10 minutes at room temperature and cells permeabilized with 0.1% Triton-X 100 (Fisher). In parallel, Huh7 2-D monolayers were seeded in 8-well chamber slides at 80% confluence and fixed 48 hours post seeding. 3-D aggregates and 2-D monolayer cells were stained with antibodies specific for scavenger receptor class B member 1 (SR-BI) (BD Biosciences, Franklin Lakes, NJ), CD81 (AbD Serotec, Raleigh, NC), CLDN1 (Abnova, Taipei, Taiwan), CD26 (Abcam, Cambridge, UK), β-Catenin (Zymed, San Francisco, CA), E-cadherin (Zymed), zona occludens 1 (ZO-1) (Zymed), Occludin (Zymed) or HCV E2 (C1 ) overnight at 4°C, followed by incubation with a 1:1,000 dilution of an appropriate Alexa555-conjugated secondary antibody (Molecular Probes, Carlsbad, CA) for 1 h at room temperature. Cell nuclei were stained by Hoechst dye. Bound antibodies were visualized via confocal microscopy (630×, Zeiss LSM 510, Germany). Images were analyzed using Zeiss LSM Alpha Imager Browser v4.0 software (Zeiss), and brightness and contrast were adjusted using Adobe® Photoshop® (San Jose, CA). Alternately, 3-D aggregates were embedded in OCT freezing medium (TissueTek, Fisher) or paraffin, sectioned and stained with Hoechst dye or Hematoxylin and Eosin (H&E), respectively.
HCV infection kinetics
Huh7 3-D aggregates were infected with JFH-1 HCVcc at an MOI of 0.01 FFU/cell at day 1, 7 or 14 post RWV seeding by injection of the viral inoculum directly into the RWV. At indicated times post infection (p.i.), medium was harvested for titration analysis and RNA was isolated from ~0.5 ml of aggregates for reverse transcription followed by RTqPCR as described above.
Infectivity titration assay
Culture supernatants were serially diluted 10-fold and used to infect triplicate Huh7 cultures in 96-well plates. At 24 h p.i., cultures were overlayed with complete DMEM containing 0.4% methylcellulose (Fluka BioChemika, Switzerland) to give a final concentration of 0.25% methylcellulose. Seventy-two hours p.i., cells were fixed in 4% paraformaldehyde (Sigma), and immunohistochemically stained for HCV E2 using the anti-HCV E2 antibody C1 . Viral titers are expressed as FFU/ml, determined by the average E2-positive foci number detected at the highest HCV-positive dilution.
Establishment of Huh7 3-D Aggregates
To assess the utility of the RWV as a culture method for Huh7 cells, Huh7 cells were cultured on Cytodex-3 microcarrier beads in the RWV for 26 days. Morphological and cytological examination of cultures demonstrated that Huh7 cells efficiently adhered to the collagen-coated microcarrier beads and that these individual beads then assembled to form 3-D tissue-like aggregates containing ~10–20 beads per aggregate (Fig. 1A). To determine if these aggregates consisted of multilayered cells, aggregates were embedded in OCT freezing medium or paraffin, sectioned, stained with either Hoechst stain (Fig. 1B) or H&E (Fig. 1C–D) and examined by fluorescence or light microscopy, respectively. Panels C and D highlight the multilayered cellular infrastructure of the Huh7 3-D aggregates, while Hoechst's staining in Panel B illustrates similar infrastructure and confirms that the aggregates are devoid of necrotic cores.
Gene Expression within Huh7 3-D Aggregates
One measure of hepatocyte differentiation is up-regulation of expression of transcription factors such as hepatocyte nuclear factors (HNF) [32, 33], which regulate the expression of liver secretory proteins  such as albumin , alpha-1-antitrypsin (α1AT; ), and transthyretin (TTR; ). Likewise, induction of enzymes and transporters involved in Phase I and II xenobiotic metabolism [37, 38], which include cytochrome P450s (CYPs) and UDP-glucuronosyltransferase (UGTs) enzymes, respectively, is another hallmark of hepatocyte differentiation. Hence, to determine whether culturing Huh7 cells in the RWV allows for cellular differentiation at the transcriptional level, expression of hepatocyte-specific genes, CYPs, and UGTs were analyzed. At indicated times post seeding, total cellular RNA was extracted from 0.5 ml of 3-D Huh7 aggregates or 2-D Huh7 monolayers grown to confluence, and relative gene expression was assessed by RTqPCR analysis. As illustrated in Fig. 2, mRNA levels for the hepatocyte-specific genes and the CYP and UGT enzymes were significantly induced in 3-D Huh7 aggregates (relative to 2-D Huh7 monolayers) and increased in a time-dependent manner while cultured in the RVW.
Expression and Organization of Cellular Tight Junction and Polarity Markers in 3-D Huh7 Aggregates
While the effect of HCV on cell polarity and TJs (and vice-versa) cannot be accurately studied in 2-D monolayer Huh7 cultures , these interactions are of particular interest as TJ proteins are involved in the entry of numerous viruses [39–41] and the TJ proteins CLDN1  and occludin [8, 9] have recently been shown to be involved in HCV entry. Therefore, we assessed the expression and organization of the HCV receptors (CD81 and SR-B1), cell adhesion molecules (E-Cadherin and β-Catenin), cellular TJ proteins (CLDN1, ZO-1 and Occuldin-1) and the polarity marker (CD26) in 3-D Huh7 aggregates and their 2-D monolayer counterparts (Fig. 3). The expression of known HCV receptors and polarity markers were increased in 3-D Huh7 aggregates as compared 2-D Huh7 monolayers, similar to that observed by Mee et al in polarized Caco-2 cells . This was not a consequence of increased mRNA levels, as normalized transcript levels for all markers examined were similar between 3-D and 2-D Huh7 cultures, as determined by RTqPCR (data not shown).
As expected, the cell adhesion molecules E-Cadherin and β-Catenin were membrane localized both in 2-D and 3-D Huh7 cultures; however, there was a profound decrease in the accumulation of nuclear β-Catenin-containing complexes in the 3-D Huh7 aggregates. Because atypical nuclear localization of β-Catenin in transformed cells has been well documented , the loss of this cancer-specific phenotype in the 3-D cultured Huh7 aggregates is consistent with the loss of cancer-specific markers observed in other continuous cell lines when cultured in the RWV [14, 23]. Additionally, in contrast to the 2-D Huh7 monolayers, TJ markers localized to apicalateral and/or basolateral planes in the 3-D Huh7 aggregates consistent with localization patterns observed in primary hepatocytes [6, 43] and normal liver tissues . Finally, CD26, a cell surface ectopeptidase that localizes to the apical plane of polarized cells , was non-detectable in 2-D Huh7 monolayers, while, apical staining of this marker was apparent in distinct areas of 3-D Huh7 aggregates (Fig. 3). Taken together, these data demonstrate that the expression and distribution of cell adhesion and TJ proteins, including known HCV entry receptors, is enhanced and more polarized in 3-D Huh7 cultures than in 2-D monolayers.
HCVcc Infection of Huh7 3-D Aggregates
Because it has been suggested that hepatocyte polarization is inversely related to the permissiveness of the cell for HCVcc infection [10, 11], we sought to determine if Huh7 3-D cultures were permissive for HCVcc infection. As such, 3-D Huh7 cultures were inoculated with HCVcc JFH-1 1, 7, or 14 days post RWV-seeding and culture supernatant and cellular RNA were harvested at various time points p.i. for titration of extracellular viral titers and RTqPCR analysis of viral RNA, respectively. Fig. 4A illustrates that HCV not only infected the Huh7 3-D aggregates, but that the kinetics of HCV RNA expansion and infectious virus production increased exponentially to levels comparable to those reported using the robust 2-D Huh7 system [2, 46]. To determine the percentage of cells expressing HCV proteins, indirect immunofluorescence analysis of infected 3-D Huh7 aggregates was performed. Fig. 4B shows that the majority of Huh7 cells were positive for the HCV E2 glycoprotein and that the entire aggregate was permissive for HCV infection rather than just the cells at the periphery, demonstrating that HCV can spread throughout the aggregates. Importantly, Fig. 4D and 4E illustrate that aggregates allowed to differentiate in the RWV for 7 or 14 days were as equally permissive for HCVcc infection as cells infected 1 day post RWV seeding (Fig. 4B-C), suggesting that differentiation and polarization does not negatively affect HCVcc infection in this 3-D cell culture model.
Here we demonstrate that Huh7 cells cultured in RWV bioreactors form multi-layered tissue-like aggregates that are phenotypically distinct from traditional Huh7 2-D monolayers (Fig. 1 and 2). Specifically, the RWV-environment promoted increases in hepatocyte-specific, as well as Phase I and II metabolic gene transcripts in 3-D Huh7 aggregates relative to Huh7 monolayers (Fig. 2). Additionally, we observed increased expression and organization of cellular HCV receptors, cell adhesion, tight junction and polarity-specific proteins, and the loss of cancer-associated nuclear localization of β-Catenin, in RWV 3-D Huh7 aggregates as compared to 2-D monolayers (Fig. 3). These data therefore suggest that the RWV environment promotes differentiation of Huh7 cells down a more hepatocyte-like route. Importantly, since these 3-D Huh7 cultures remain highly permissive for HCVcc infection, this system represents a new in vitro cell culture system for the study of HCV infection and antiviral drug studies in more polarized, xenobiotically-competent cells.
Relevant to the study of HCV, expression of the HCV receptors CD81 and SR-B1 were both diffuse and poorly organized in 2-D cultured Huh7s cells, while their expression was increased and localized to apical TJ regions and basolateral-sinusoidal surfaces in 3-D aggregates. Likewise, TJ proteins, which typically localize to the apical surface in polarized hepatocytes , were more concentrated at the apical surface of 3-D Huh7 aggregates as compared to monolayer cultures. Notably however, the TJ protein CLDN1, a recently identified HCV receptor , not only localized to TJs, but was also present at both apical and basolateral surfaces in 3-D aggregates. This localization pattern is in agreement with other studies  and the model proposed by Reynolds et al., describing tight-junctional (apical) and nonjunctional (basolateral) forms of CLDN1 in polarized hepatocytes . As suggested by Mee et al, it may be that these non-junctional pools of CLDN1 have a direct role in HCV entry . Interestingly, Battle et al., have demonstrated a correlation between HNF4α and cell adhesion and TJ molecules expression and organization . Whether this is also the case in the 3-D Huh7 aggregates, which have increased HNF4α expression (Fig. 2A) remains to be determined. Nonetheless, the ability of 3-D cultured Huh7 cells to better organize cell adhesion and TJ proteins is a phenotype consistent with other RWV-cultured cell types [14, 21, 23]. As such, RWV-cultured Huh7 cells provide an appropriate model for investigating HCV entry, particularly the interaction, organization, and stoichiometry of HCV receptors and TJ proteins. Additional analyses to determine the extent of differentiation and polarization of 3-D Huh7 aggregates is still warranted and a focus of ongoing studies.
To date, attempts to study HCV in polarized cells have been limited to colorectal adenocarcinoma Caco-2 cells  or HepG2 cells , neither of which support robust HCVcc infection. Although an inverse relationship between cell polarization and HCV entry into polarized Caco-2  and HepG2  cells has been observed no such phenotype was observed in 3-D Huh7 aggregates. Specifically, 3-D Huh7 aggregates, infected at various stages of differentiation (e.g. day 1, 7 or 14 post seeding), were equally permissive for HCVcc infection (Fig. 4B–E). Furthermore, 3-D aggregates treated with PMA, a known disruptor of TJ formation , were no more permissive for HCV infection as compared to untreated parallel aggregates (data not shown), suggesting that the TJ barriers formed in 3-D Huh7 aggregates are not inhibitory for HCVcc infection.
Growing evidence suggests interplay between TJ protein expression, localization and function and HCV infection. Although, the current HCV infectious 2-D Huh7 cell culture system does not amend itself well to elucidating these dynamic relationships, the highly HCV-permissive 3-D Huh7 cell culture system described herein more closely mimics the differentiated and polarized state of hepatocytes. As such the RWV 3-D Huh7 cell culture system should prove useful for understanding the dynamic relationship between HCV and TJ protein expression as well as elucidating how HCV interacts with and disrupts key aspects of hepatocyte physiology.
hepatitis C virus
Japanese Fulminant Hepatitis
rotating wall vessel
hepatitis C virus cell-cultured produced
multiplicity of infection
focus forming units
real-time quantitative PCR
scavenger receptor class B member 1
zona occludens 1
hematoxylin and eosin
hepatocyte nuclear factors
Chisari FV: Unscrambling hepatitis C virus-host interactions. Nature 2005, 436: 930-932.
Zhong J, Gastaminza P, Cheng G, Kapadia S, Kato T, Burton DR, Wieland SF, Uprichard SL, Wakita T, Chisari FV: Robust hepatitis C virus infection in vitro. Proc Natl Acad Sci USA 2005, 102: 9294-9299.
Lindenbach BD, Evans MJ, Syder AJ, Wolk B, Tellinghuisen TL, Liu CC, Maruyama T, Hynes RO, Burton DR, McKeating JA, Rice CM: Complete replication of hepatitis C virus in cell culture. Science 2005, 309: 623-626.
Wakita T, Pietschmann T, Kato T, Date T, Miyamoto M, Zhao Z, Murthy K, Habermann A, Krausslich HG, Mizokami M, Bartenschlager R, Liang TJ: Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 2005, 11: 791-796.
Moradpour D, Penin F, Rice CM: Replication of hepatitis C virus. Nat Rev Microbiol 2007, 5: 453-463.
Decaens C, Durand M, Grosse B, Cassio D: Which in vitro models could be best used to study hepatocyte polarity? Biol Cell 2008, 100: 387-398.
Evans MJ, von Hahn T, Tscherne DM, Syder AJ, Panis M, Wolk B, Hatziioannou T, McKeating JA, Bieniasz PD, Rice CM: Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry. Nature 2007, 446: 801-805.
Liu S, Yang W, Shen L, Turner JR, Coyne CB, Wang T: Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfection. J Virol 2009, 83: 2011-2014.
Ploss A, Evans MJ, Gaysinskaya VA, Panis M, You H, de Jong YP, Rice CM: Human occludin is a hepatitis C virus entry factor required for infection of mouse cells. Nature 2009, 457: 882-886.
Mee CJ, Grove J, Harris HJ, Hu K, Balfe P, McKeating JA: Effect of cell polarization on hepatitis C virus entry. J Virol 2008, 82: 461-470.
Mee CJ, Harris HJ, Farquhar MJ, Wilson G, Reynolds G, Davis C, van ISC, Balfe P, McKeating JA: Polarization restricts hepatitis C virus entry into HepG2 hepatoma cells. J Virol 2009, 83: 6211-6221.
Schwarz RP, Goodwin TJ, Wolf DA: Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity. J Tissue Cult Methods 1992, 14: 51-57.
Nickerson CA, Goodwin TJ, Terlonge J, Ott CM, Buchanan KL, Uicker WC, Emami K, LeBlanc CL, Ramamurthy R, Clarke MS, Vanderburg CR, Hammond T, Pierson DL: Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis. Infect Immun 2001, 69: 7106-7120.
Lamarca HL, Ott CM, Honer Zu Bentrup K, Leblanc CL, Pierson DL, Nelson AB, Scandurro AB, Whitley GS, Nickerson CA, Morris CA: Three-dimensional growth of extravillous cytotrophoblasts promotes differentiation and invasion. Placenta 2005, 26: 709-720.
Goodwin TJ, Prewett TL, Wolf DA, Spaulding GF: Reduced shear stress: a major component in the ability of mammalian tissues to form three-dimensional assemblies in simulated microgravity. J Cell Biochem 1993, 51: 301-311.
Gomez-Lechon MJ, Donato MT, Castell JV, Jover R: Human hepatocytes in primary culture: the choice to investigate drug metabolism in man. Curr Drug Metab 2004, 5: 443-462.
Reid LM, Fiorino AS, Sigal SH, Brill S, Holst PA: Extracellular matrix gradients in the space of Disse: relevance to liver biology. Hepatology 1992, 15: 1198-1203.
Zeilinger K, Sauer IM, Pless G, Strobel C, Rudzitis J, Wang A, Nussler AK, Grebe A, Mao L, Auth SH, Unger J, Neuhaus P, Gerlach JC: Three-dimensional co-culture of primary human liver cells in bioreactors for in vitro drug studies: effects of the initial cell quality on the long-term maintenance of hepatocyte-specific functions. Altern Lab Anim 2002, 30: 525-538.
Freshney RI: Culture of animal cells: a manual of basic technique. 4th edition. New York, N.Y.: Wiley-Liss; 2000.
Unsworth BR, Lelkes PI: Growing tissues in microgravity. Nat Med 1998, 4: 901-907.
Hammond TG, Hammond JM: Optimized suspension culture: the rotating-wall vessel. Am J Physiol Renal Physiol 2001, 281: F12-25.
Khaoustov VI, Risin D, Pellis NR, Yoffe B: Microarray analysis of genes differentially expressed in HepG2 cells cultured in simulated microgravity: preliminary report. In Vitro Cell Dev Biol Anim 2001, 37: 84-88.
Carterson AJ, Honer zu Bentrup K, Ott CM, Clarke MS, Pierson DL, Vanderburg CR, Buchanan KL, Nickerson CA, Schurr MJ: A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect Immun 2005, 73: 1129-1140.
Walther I: Space bioreactors and their applications. Adv Space Biol Med 2002, 8: 197-213.
Gastaminza P, Kapadia SB, Chisari FV: Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles. J Virol 2006, 80: 11074-11081.
Zhong J, Gastaminza P, Chung J, Stamataki Z, Isogawa M, Cheng G, McKeating JA, Chisari FV: Persistent hepatitis C virus infection in vitro: coevolution of virus and host. J Virol 2006, 80: 11082-11093.
Sainz B Jr, Barretto N, Uprichard SL: Hepatitis C Virus infection in phenotypically distinct Huh7 cell lines. PLoS ONE 2009, in press.
Uprichard SL, Chung J, Chisari FV, Wakita T: Replication of a hepatitis C virus replicon clone in mouse cells. Virol J 2006, 3: 89.
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162: 156-159.
Choi S, Sainz B Jr, Corcoran P, Uprichard SL, Jeong H: Characterization of increased drug metabolism activity in dimethyl sulfoxide (DMSO)-treated Huh7 hepatoma cells. Xenobiotica 2009, 39: 205-217.
Law M, Maruyama T, Lewis J, Giang E, Tarr AW, Stamataki Z, Gastaminza P, Chisari FV, Jones IM, Fox RI, Ball JK, McKeating JA, Kneteman NM, Burton DR: Broadly neutralizing antibodies protect against hepatitis C virus quasispecies challenge. Nat Med 2008, 14: 25-27.
Jochheim A, Hillemann T, Kania G, Scharf J, Attaran M, Manns MP, Wobus AM, Ott M: Quantitative gene expression profiling reveals a fetal hepatic phenotype of murine ES-derived hepatocytes. Int J Dev Biol 2004, 48: 23-29.
Nacer-Cherif H, Bois-Joyeux B, Rousseau GG, Lemaigre FP, Danan JL: Hepatocyte nuclear factor-6 stimulates transcription of the alpha-fetoprotein gene and synergizes with the retinoic-acid-receptor-related orphan receptor alpha-4. Biochem J 2003, 369: 583-591.
Kamiya A, Kinoshita T, Ito Y, Matsui T, Morikawa Y, Senba E, Nakashima K, Taga T, Yoshida K, Kishimoto T, Miyajima A: Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer. Embo J 1999, 18: 2127-2136.
Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PG, Irving JA, Lomas DA, Luke CJ, Moyer RW, Pemberton PA, Remold-O'Donnell E, Salvesen GS, Travis J, Whisstock JC: The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature. J Biol Chem 2001, 276: 33293-33296.
Dickson PW, Aldred AR, Menting JG, Marley PD, Sawyer WH, Schreiber G: Thyroxine transport in choroid plexus. J Biol Chem 1987, 262: 13907-13915.
Nakata K, Tanaka Y, Nakano T, Adachi T, Tanaka H, Kaminuma T, Ishikawa T: Nuclear receptor-mediated transcriptional regulation in Phase I, II, and III xenobiotic metabolizing systems. Drug Metab Pharmacokinet 2006, 21: 437-457.
Martinez-Jimenez CP, Jover R, Donato MT, Castell JV, Gomez-Lechon MJ: Transcriptional regulation and expression of CYP3A4 in hepatocytes. Curr Drug Metab 2007, 8: 185-194.
Connolly-Andersen AM, Magnusson KE, Mirazimi A: Basolateral entry and release of Crimean-Congo hemorrhagic fever virus in polarized MDCK-1 cells. J Virol 2007, 81: 2158-2164.
Coyne CB, Bergelson JM: Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions. Cell 2006, 124: 119-131.
Galen B, Cheshenko N, Tuyama A, Ramratnam B, Herold BC: Access to nectin favors herpes simplex virus infection at the apical surface of polarized human epithelial cells. J Virol 2006, 80: 12209-12218.
Levrero M: Viral hepatitis and liver cancer: the case of hepatitis C. Oncogene 2006, 25: 3834-3847.
Stevenson BR, Keon BH: The tight junction: morphology to molecules. Annu Rev Cell Dev Biol 1998, 14: 89-109.
Reynolds GM, Harris HJ, Jennings A, Hu K, Grove J, Lalor PF, Adams DH, Balfe P, Hubscher SG, McKeating JA: Hepatitis C virus receptor expression in normal and diseased liver tissue. Hepatology 2008, 47: 418-427.
Darmoul D, Lacasa M, Baricault L, Marguet D, Sapin C, Trotot P, Barbat A, Trugnan G: Dipeptidyl peptidase IV (CD 26) gene expression in enterocyte-like colon cancer cell lines HT-29 and Caco-2. Cloning of the complete human coding sequence and changes of dipeptidyl peptidase IV mRNA levels during cell differentiation. J Biol Chem 1992, 267: 4824-4833.
Sainz B Jr, Chisari FV: Production of infectious hepatitis C virus by well-differentiated, growth-arrested human hepatoma-derived cells. J Virol 2006, 80: 10253-10257.
Rahner C, Mitic LL, Anderson JM: Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut. Gastroenterology 2001, 120: 411-422.
Battle MA, Konopka G, Parviz F, Gaggl AL, Yang C, Sladek FM, Duncan SA: Hepatocyte nuclear factor 4alpha orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver. Proc Natl Acad Sci USA 2006, 103: 8419-8424.
Schmitt M, Horbach A, Kubitz R, Frilling A, Haussinger D: Disruption of hepatocellular tight junctions by vascular endothelial growth factor (VEGF): a novel mechanism for tumor invasion. J Hepatol 2004, 41: 274-283.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25: 402-408.
We thank Drs. Heather L. LaMarca and Kerstin Hönzer zu Bentrup for helpful discussions, Dr. Francis Chisari for Huh7 cells, Dr. Takaji Wakita for the JFH-1 containing plasmid (pJFH-1), Dr. Dennis Burton for the monoclonal anti-HCV E2 human antibody (C1), Dr. Mei Ling Chen for assistance with confocal microscopy and Patricia A. Mavrogianis for paraffin embedding and sectioning of 3-D aggregates.
This work was supported by Public Health Service grant AI-070827 from the National Institute of Allergy and Infectious Diseases, Public Health Service grant CA-133266 from the National Cancer Institute and the University of Illinois Chicago Council to support Gastrointestinal and Liver Disease (UIC GILD). VTC was supported by an Institutional Ruth L. Kirchstein National Research Service Award (DK-007788-07) from the National Institute of Diabetes and Digestive and Kidney Diseases.
The authors declare that they have no competing interests.
BS and VT participated in the design of the study, performed the experiments and drafted the manuscript. SLU designed the study and participated in drafting the manuscript. All authors read and approved the final manuscript.
Bruno Sainz Jr, Veronica TenCate contributed equally to this work.
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Sainz, B., TenCate, V. & Uprichard, S.L. Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection. Virol J 6, 103 (2009). https://doi.org/10.1186/1743-422X-6-103
- Huh7 Cell
- Tight Junction Protein
- Rotate Wall Vessel
- Tight Junction Protein Expression
- Rotate Wall Vessel Bioreactor