Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection

Figure 2

Increased differentiation-specific gene expression in 3-D Huh7 RWV aggregates. At indicated time points post seeding, 0.5 ml aliquots of 3-D Huh7 aggregates (~5 × 104 cells) were removed from the RWV, pelleted at 1400 RPM for 5 minutes and total RNA extracted. Expression of (A) hepatocyte-specific genes, (B) Phase I (CYP) and (C) Phase II (UGT) metabolic genes in Huh7 3-D aggregates was assessed by RTqPCR. Expression of each transcript, relative to 2-D Huh7 monolayer cultures, was determined using the method [50], by normalizing to β-actin expression and is graphed as fold induction compared to 2-D monolayers.

Back to article page