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Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis
https://doi.org/10.1186/1743-422X-4-81
© Hussain et al; licensee BioMed Central Ltd. 2007
- Received: 04 July 2007
- Accepted: 29 August 2007
- Published: 29 August 2007
Abstract
Background
The human immunodeficiency virus type 1(HIV-1) is a complex retrovirus and the causative agent of acquired immunodeficiency syndrome (AIDS). The HIV-1 Vpu protein is an oligomeric integral membrane protein essential for particle release, viral load and CD4 degradation. In silico models show Vpu to form pentamers with an ion channel activity.
Results
Using Vpu proteins from a primary subtype C and the pNL4-3 subtype B isolates of HIV-1, we show oligomerization of the full-length protein as well as its transmembrane (TM) domain by genetic, biochemical and biophysical methods. We also provide direct evidence of the presence of Vpu pentamers in a stable equilibrium with its monomers in vitro. This was also true for the TM domain of Vpu. Confocal microscopy localized Vpu to the endoplasmic reticulum and Golgi regions of the cell, as well as to post-Golgi vesicles. In fluorescence resonance energy transfer (FRET) experiments in live cells we show that Vpu oligomerizes in what appears to be either the Golgi region or intracellular vesicles, but not in the ER.
Conclusion
We provide here direct evidence that the TM domain, is critical for Vpu oligomerization and the most favourable channel assembly is a pentamer. The Vpu oligomerization appears to be either the Golgi region or intracellular vesicles, but not in the ER.
Keywords
- Fluorescence Resonance Energy Transfer
- Simian Immunodeficiency Virus
- Maltose Binding Protein
- Golgi Region
- Fluorescence Resonance Energy Transfer Analysis
Background
Primate lentiviruses, including HIV, encode a number of accessory proteins that perform essential functions during the viral life cycle [1]. One such protein is viral protein U (Vpu) that is encoded by HIV-1 but not HIV-2 or the simian immunodeficiency virus (SIV) [2]. Certain HIV-2 isolates have been shown to possess a partial Vpu-like activity in their envelope glycoprotein [3, 4]. Whether Vpu is a viral pathogenesis factor remains to be established, but compared to HIV-1, closely related retroviruses such as HIV-2 and SIV that lack expression of a fully functional Vpu protein also cause less severe disease outcomes.
The Vpu protein of HIV-1 is an 81-amino acid type I integral membrane protein in which a short extracellular N-terminus is followed by a transmembrane (TM) domain and a cytoplasmic domain, the latter with two prominent alpha helices [2, 5]. The region between the two helices, is highly conserved and contains two serine residues (S52 and S56) that are phosphorylated by cellular casein kinase II [6]. Two primary functions have been attributed to Vpu during the HIV-1 replication cycle. These include CD4 downmodulation and enhancement of viral particle release [7].
Like all retroviruses, HIV-1 also interferes with the expression of its cellular receptor and uses redundant mechanisms to achieve this [8]. The Vpu protein binds CD4 in the endoplasmic reticulum (ER) [9], and through its phosphoserine residues binds the beta transducin-repeat containing protein (βTrCP) in the cytoplasm [10]. The βTrCP recruits other proteins such as Skp1, Cul-1 and the Cdc34 E2 ubiquitin ligase [11]. This results in ubiquitination of CD4, its dislocation from the ER and degradation by the proteosome [12, 13]. The stable association of Vpu with βTrCP also affects the latter's cellular functions, one of which is to direct the proteosomal degradation of inhibitor of kappa B (IκB) [14]. This results in inhibition of NFκB activity and the NFκB-dependent expression of anti-apoptotic genes of the Bcl-2 family [15]. Vpu also mediates the efficient release of viral particles from HIV-1-infected cells [16]. Though distinct from its CD4 degradation function, it is not clear whether Vpu enhances virus release through modification of the cellular environment or specific interactions with cellular or viral factors. The formation of conducting ion channels by Vpu [17] and its interaction with a novel tetratricopeptide repeat containing protein [18] favour both possibilities. The TM domain of Vpu has been shown to be important for enhancement of virus release [19, 20] and pathogenicity [21]. This domain is also critical for its ion channel activity [13, 17].
An earlier study has used chemical cross-linking to show that Vpu can form oligomers [22]. In silico modeling studies have predicted the same for the TM domain of Vpu [23]. The oligomeric nature of Vpu is also likely to affect its interaction with cellular proteins and therefore its role in HIV-1 pathogenesis. To better characterize the oligomerization of Vpu and the domains for this, we have used genetic, biochemical and biophysical methods, and two divergent Vpu proteins, one from a subtype C primary isolate and the other from a laboratory-adapted subtype B isolate of HIV-1. Our results show that Vpu or its TM domain form pentamers in solution. Using confocal microscopy and fluorescence resonance energy transfer experiments we further show that Vpu does not oligomerize in the ER, but does so in the Golgi region or in post-golgi vesicles.
Results
Cloning and expression of a functional Vpu
Cloning and expression of functional Vpu. (A) An alignment of the NL-Vpu (subtype B) and R5-Vpu (subtype C) protein sequences is shown. Boxes show sequence conservation; dark shading indicates sequence identity and light shading indicates conservative changes. The transmembrane domain, cytoplasmic helices and phosphoserine residues (black circles) are indicated. Residues are numbered according to the NL-Vpu sequence. (B) Phylogenetic comparison of the NL and R5 Vpu protein sequences to the consensus Vpu sequences for different HIV-1 subtypes available in the Los Alamos HIV sequence database http://www.hiv.lanl.gov/content/hiv-db/ALIGN_CURRENT/ALIGN-INDEX.html. The tree was drawn using the ClustalW (V1.4) algorithm in MacVector v7.2.2 (Accelrys). A Blosum similarity matrix was used with the following settings: Open Gap Penalty = 10; Extend Gap Penalty = 0.1; Delay Divergent = 40%; Gap Distance = 8. Numbers indicate the relative distance. (C) HeLa cells were transfected with wild type (Vpu+) or vpu-deficient (Vpu-) HIV-1 proviral DNA, the latter with empty vector (lanes 2 and 5) or an R5-Vpu expression vector (lanes 3 and 6), as described in Methods. Virions released in the culture medium (lanes 1–3) and intracellular virions (lanes 4–6) were estimated by western blotting with anti-p24 antibodies. The bands corresponding to the HIV-1 capsid precursors p55 and p41, and the mature p24 are indicated.
Homotypic and heterotypic interactions of full-length and truncated Vpu proteins
Yeast two-hybrid analysis. (A) Representative plates showing homotypic interactions of the R5-Vpu protein. The first panel shows the template for the remaining panels that in turn show transformants streaked in each section of the indicated plates. Growth is seen as light streaks on a dark background, except in the β-galactosidase filter assay where signal is seen as dark streaks on a light background. (B) Complete results for the entire screen using NL-Vpu and R5-Vpu full-length, transmembrane domain and cytoplasmic domain fusions to the Gal4 protein DNA-binding domain (BD) or activation domain (AD). Growth (+) or no growth (-) of transformants on various media is shown. LTH-3AT represents growth on SDLeu- Trp-His- plates containing 20 mM 3-amino-1,2,3-triazole. The β-galactosidase filter assay results are indicated as + or - and the liquid β-galactosidase assay values are shown in parentheses as an average of two independent measurements. Various negative and positive controls are also shown.
A more extensive screen, whose results are summarized in Fig. 2B, was carried out as above. Both NL Vpu and R5 Vpu showed homotypic interactions. Further, the NL Vpu and R5 Vpu proteins interacted with each other. All these interactions were found to depend upon the TM domains, but not on the cytoplasmic domains of Vpu. The transformants were grown in the presence of 20 mM 3-aminotriazole (3AT) to further confirm the specificity and strength of the interactions. All cotransformants that grew on SD/LTH- plates also grew on SD/LTH- 3AT plates. Compared to the positive control (BD/SNF1+AD/SNF4), the semi-quantitative liquid β-galactosidase assay showed reasonably strong interactions between the full-length Vpu proteins and slightly weaker interactions between their TM domains. The values were higher for homologous interactions (NL vs NL and R5 vs R5) as opposed to heterologous interactions (NL vs R5). A variety of negative controls showed no interaction and only background β-galactosidase activity.
Gel electrophoresis and in vitro binding assays
Vpu oligomerization based on gel electrophoretic and pull-down assays. The full-length R5-Vpu protein and its transmembrane and cytoplasmic domains were synthesized and labeled with 35S-methionine in a coupled in vitro transcription-translation system. (A) The proteins were analyzed on native polyacrylamide gels without heating or DTT treatment. Lanes 1,3 and 5, markers; lanes 2,4 and 6, Vpu full-length, TM domain and cytoplasmic domain, respectively. The molecular sizes (in kilodaltons) are indicated. (B) For pull-down assays, the 35S-labeled R5-Vpu proteins were synthesized in vitro and bound to amylose beads saturated with either the maltose binding protein (MBP)/R5-Vpu fusion protein or MBP alone as a control. The beads were washed, resuspended in loading dye buffer, boiled and the supernatants subjected to SDS-PAGE. The gels were dried and autoradiographed. Gels show the full-length (FL) R5-Vpu protein, or its transmembrane (TM) or cytoplasmic (cyto) domains, retained on the beads. Arrows indicate the full-length or truncated Vpu proteins.
Vpu forms a pentamer in vitro
Gel permeation analysis of Vpu oligomers. In vitro translated and 35S-labeled Vpu proteins were separated by gel permeation chromatography as described in Methods. The eluted 35S counts in each fraction are indicated for the full-length (black circles) or TM domain (black squares) Vpu proteins. The positions of Vpu monomers and oligomers are indicated. The inset shows SDS-PAGE analysis of the peak fractions. Lanes: 1, Vpu oligomer; 2, Vpu monomer; 3, TM domain oligomer; 4, TM domain monomer; lane M shows molecular size marker as indicated (in kilodaltons).
Subcellular localization and FRET analysis
Subcellular localization of Vpu. U2-OS cells were cotransfected as described in Methods. The individual and merged images are shown. In the merged image, magenta and white arrows indicate Vpu/ER and Vpu/Golgi colocalizations, respectively.
FRET analysis of Vpu interactions. COS-1 and U2-OS cells were cotransfected with Vpu-ECFP and Vpu-EYFP expression vectors and FRET was performed as described in Methods. Representative images for COS-1 and U2-OS cells are shown together with a kinetic profile of the FRET experiment. For U2-OS cells, sets A and B represent the same cell, with FRET carried out in two different regions of colocalization indicated by the box marked 1; the box marked 2 represents a control region of the cell. A total of 4 independent FRET experiments were carried out in 7–10 different cotransfected cells each time.
We carried out FRET measurements in live cells showing various patterns of Vpu subcellular distribution. Two different areas within the same cell, one showing colocalization and another where no colocalization was observed were subjected to FRET analysis. As expected (and seen in the merged image), the Vpu-ECFP and Vpu-EYFP proteins colocalized in transfected cells (Fig. 6; upper panels). On simultaneous scanning of the two fluorophores, there was an increase in cyan (donor) fluorescence following bleaching of the yellow (acceptor) fluorophore. Multiple FRET measurements were carried out in more than one region of the same cell with similar results (not shown). In COS-1 cells showing this pattern of Vpu distribution, the mean fluorescence intensities of ECFP-Vpu before and after EYFP-Vpu photobleaching were 111.95 ± 5.7 and 137.30 ± 6.2, respectively. This gave an average FRET efficiency of 18.5%. On the other hand, no FRET was observed in the ER region in COS-1 cells expressing high levels of tagged Vpu proteins (Fig. 6; middle panels). In U2-OS cells, the FRET analysis was carried out in two separate regions of the same transfected cells (Fig. 6; lower panels). No FRET was measured in the intensely staining ER region (Fig. 6, set A). However, FRET between ECFP-Vpu and EYFP-Vpu was observed reproducibly in regions of the cell that appeared to be either Golgi or unidentified vesicles (Fig. 6, set B). The mean fluorescence intensities of ECFP-Vpu before and after EYFP-Vpu photobleaching were 70.29 ± 4.26 and 95.04 ± 4.8, respectively. This gave an average FRET efficiency of 26%. For technical limitations in the imaging, it was not possible to also cotransfect these cells with subcellular markers to positively identify, in the same cell, those subcellular structures that support Vpu-Vpu FRET and those that do not. Overall, our FRET experiments provide strong in vivo evidence of Vpu-Vpu interaction in a live cell. Further, based on these results, Vpu appears to form oligomers in distinct subcellular locations, primarily in the Golgi and vesicular regions but not in the ER.
Discussion
Lentiviruses encode a number of unique accessory proteins that are important for viral replication and pathogenesis, but are not encoded by other retroviruses. The versatility of HIV accessory proteins arise from their ability to function as adaptor molecules that connect various viral and cellular proteins to pre-existing cellular pathways, modulate these pathways and control processes important for viral replication. Thus, protein-protein interactions are an important aspect of the functioning of these proteins. The Vpu protein of HIV-1 is known to bind CD4 and βTRCP, the latter being a component of the E3 ubiquitin ligase complex. This association is instrumental in dislocation of CD4 from the ER, its ubiquitination and subsequent degradation by the proteosome [10]. The other function of Vpu is to promote the release of progeny viruses from infected cells. This appears to be dependent upon the ability of Vpu to form oligomeric complexes with an ion channel activity in cellular membranes [7].
The nature of the Vpu oligomer is important due to its functional significance in virion release. An earlier study used chemical cross-linking to demonstrate the oligomerization of Vpu [22]. Here we have used various genetic, biochemical and biophysical approaches to characterize the oligomerization of Vpu in vitro and in vivo. Molecular dynamic simulations and conductance studies have shown that the Vpu TM domain is sufficient for its ion channel activity [26] and the pattern of channel activity is characteristic of the self-assembly of conductive oligomers in the membrane [27]. This suggested that the TM domain of Vpu would also be required for its oligomerization. We provide here direct evidence that the hydrophobic N-terminal TM domain, and not the charged cytoplasmic domain, is critical for Vpu oligomerization. The two-hybrid and MBP pull-down analyses further showed that full-length Vpu proteins as well as their TM domains derived from two different HIV-1 subtypes interacted efficiently with each other. The isolated TM domains also showed stable interaction with the full-length Vpu protein. Molecular-dynamic simulations of ion channels formed by the Vpu TM domain predict the most favourable channel assembly to be a pentamer, but higher and lower oligomeric species were also predicted [23, 28]. Recently, Becker et al [29] have used synthetic proteins containing a carrier template to which four or five peptides corresponding to the Vpu TM domain were attached, to demonstrate ion channel formation by oligomerization of the TM domain. In our analysis, multimeric species, including pentamers were evident on gel electrophoresis of the in vitro synthesized Vpu proteins and their TM domains. Further, molecular sizing of in vitro synthesized Vpu by gel permeation chromatography clearly showed it to assemble into a pentameric species. Thus, while complementing earlier studies [23, 28, 29] we provide direct evidence for pentamerization of the full-length Vpu protein as well as its TM domain. In vitro synthesized Vpu proteins, in the absence of other cellular components, demonstrated a stable equilibrium between monomers and pentamers. This demonstrates the inherent ability of Vpu to oligomerize and pentamers appear to be the thermodynamically most stable form of these oligomers.
Earlier studies have shown Vpu to be localized to the perinuclear region of the cell that includes the ER and Golgi [16, 20]. Using CD4-Vpu fusion proteins and endoglycosidase H resistance, an earlier study has provided evidence for Vpu movement beyond the ER [30]. A similar fusion protein was also used to tease out the apoptotic pathway [15]. Recently, the imaging of a Vpu-EGFP fusion protein has also localized it to the ER, Golgi and plasma membrane [31]. So, there is evidence that Vpu has the ability to be transported to post-ER membranes. Several models have been proposed for a role of the Vpu channel in the budding of new virions [27]. It has been suggested that oligomerization of Vpu at the ER could form conducting channels leading to a collapse in the membrane potential across the ER cisternae and acceleration of membrane fusion and protein traffic in the exocytic pathway. Alternatively, at the ER/mitochondrial junctions, Vpu is proposed to collapse of the mitochondrial membrane potential and promote apoptosis. It is also possible that Vpu channels in the plasma membrane may attenuate the cell resting potential, promoting the fusion and release of new virions. We have used confocal microscopy and FRET analysis to test these models. Using transfected fluorescent protein-tagged Vpu fusion proteins and subcellular markers we show that Vpu localizes to the ER and Golgi regions but not to the mitochondria. In the absence of its mitochondrial localization, it would be difficult to support a direct effect of Vpu on the mitochondrial pore transition complex or transmembrane potential [32]. One possibility is the effect of Vpu channels on Ca2+ release from its intracellular stores in the ER [33]. The FRET analysis in this study showed no oligomerization of Vpu associated with the ER, arguing against the role of ER directly or indirectly in this process.
Oligomerization of Vpu was observed by FRET in structures that were distal to the ER. While these structures could not be identified positively in the same cell due to the technical limitations, separate stainings in two different cell types (COS-1 and U2-OS) suggested that these structures might either be Golgi or vesicles associated with exocytic protein transport. The Vpu ion channel activity would be detrimental to ER function, inducing ER toxicity, stress and apoptosis of the infected cell. This would go against the plan of survival of productively infected cells followed by retroviruses and so nicely exemplified by HIV [34]. It would therefore make sense for Vpu oligomerization to occur downstream of the ER, enroute to the plasma membrane. Our FRET analysis in live cells supports this model. Since the cytoplasmic and not the transmembrane domain of Vpu is required for CD4 relocation from the ER and its subsequent degradation [20], this scheme would not affect the CD4 downmodulation function of Vpu. However, contrasting results have recently been presented wherein Vpu with a scrambled transmembrane domain was unable to downmodulate CD4 from the surface of transfected cells [21]. Whether this is due to the inability of mutant Vpu to oligomerize, or due to an altered protein structure or its arrangement in the membrane, remains to be seen.
Conclusion
We have used genetic, biochemical and biophysical methods to complement earlier studies on Vpu oligomerization and the role of its N-terminal transmembrane domain in this oligomerization. While theoretical modeling studies [23, 28] and synthetic peptides [29] had earlier predicted pentameric Vpu channels, we provide here direct evidence for the existence of a Vpu pentamer in stable equilibrium with its monomer. This was also true for the Vpu transmembrane domain. Finally, subcellular localization and FRET analysis argue against an earlier model of Vpu-mediated virion release based on channel formation in the ER. Besides channel formation and its effect on virion release, oligomerization would also influence the ability of Vpu to interact with host cell proteins towards regulating the intracellular environment for efficient viral replication, assembly and release. We are currently targeting this aspect of Vpu biology by screening for novel cellular partners.
Methods
Cloning and expression of vpu
The vpu gene was PCR amplified with Pfu polymerase (Stratagene, La Jolla, USA) using as template either the pNL4-3 plasmid DNA (NIH AIDS Research and Reference Reagent Program) or a 3.5 kb fragment encompassing the vpr to env region previously amplified and cloned from a primary isolate of HIV-1 subtype C (SRD, unpublished). The PCR primers used were as follows (with the restriction sites in italics): For the NL4-3 vpu gene, Vpu-NL-F, GGATCC ATGCAACCTATAATAGTA GCAATA and Vpu-NL-R, GAATTC ACTACAGATCATCAATATCCCAAG; for the R5 vpu gene, Vpu-R5-F, GGATCC ATGTTAAATTTAGATTATAAATTAGGAGTA GG and Vpu-R5-R, GAATTC ATTACAAATCATTAACATCCAAAAGCC. The amplified fragments designated as NL vpu and R5 vpu respectively, were cloned in plasmid pGEMT-Easy (Promega, Madison, USA) and sequenced in both directions. The gene fragments corresponding to the transmembrane (TM) domains were assembled from the following synthetic oligonucleotides: For the NL4-3 vpu TM region, NL-TM-F, CATGGAGATGCAACCTATAATAGTAGCAATAGTAGCATT AGTAGTAGCAATAATAATAGCAATAGCTGTGTGGTCCATAGTAATCATAGAATAGG and NL-TM-R, AATTCCTATTCTATGATTACTATGGACCACACAGCTATTGCTATTATTATTGCTACTACTAATGCTACTATTGCTACTATTATAGGTTGCATCTC; for the R5 vpu TM region, R5-TM-F, CATGGAGATGTTAAATTTAGATTATAAATTAGGAGTAGGAGCATTGATAGTAGCACTAATCATAGCAATAGTCGTGTGGACCATAGTATATATAGAATAGG and R5-TM-R, AATT CCTATTCTATATATACTATGGTCCACACGACTATTGCTATGATTAGTGCTA CTATCAATGCTCCTACTCCTAATTTATAATCTAAATTTAACATCTC. The cytoplasmic domains were PCR amplified using specific primers for NL vpu and R5 vpu as follows: for the NL vpu cytoplasmic region, NL-Cyto-F, CCATGG AGTATAGGAAAATATTAAGA and Vpu-NL-R (shown above); for the R5 vpu cytoplasmic region, R5-Cyto-F, CCATGG AGTATAGGAAATTGGTACAAC and Vpu-R5-R (shown above).
Vpu functional assay
Eighteen to 24 hr prior to transfection, 0.3 × 106 HeLa cells were plated per 60 mm dish. These were cotransfected with 2 μg of either wild type or vpu-deficient HIV-1 proviral DNA and 0.5 μg of the expression vector pEGFP/R5-Vpu using Lipofectin (Clontech). As a control, the empty vector pEGFP-N1 was used. Twenty-four hr post-transfection, the released virions in the culture medium were pelleted through a 20% sucrose cushion for 2 hr at 100,000 × g in a Beckman SW41 rotor. The pelleted virions and harvested cells were lysed in Laemmli sample buffer. Proteins were separated by electrophoresis on SDS-10% polyacrylamide gels and western blotted with an anti-p24 antibody.
Yeast two-hybrid assays
The GAL4-based two-hybrid system contained the DNA binding domain vector pGBKT7 and the activation domain vector pGADT7. The NL and R5 vpu genes were cloned into the pGBKT7 and pGADT7 vectors as EcoRI-BamHI fragments from the pGEMT-Easy clones. The nucleotide sequences corresponding to the TM and cytoplasmic domains of the Vpu proteins were similarly cloned in the two-hybrid vectors as NcoI-BamHI fragments, respectively. Expression of the relevant fusion proteins from each of the Vpu two-hybrid constructs was checked in a T7 polymerase based in vitro coupled transcription-translation system (Promega, Madison, USA) followed by immunoprecipitation with anti-Vpu antibodies. The yeast two-hybrid analysis was essentially carried out as described earlier [35, 36]. Plasmids pGBKT7-vpu (BD/NL or BD/R5) and pGADT7-vpu (AD/NL or AD/R5) plasmids were cotransfected into Saccharomyces cerevisiae strain AH109 (MAT a trp1-901 his3 leu2-3, 112 ura3-52 ade2 gal4 gal80URA3::GAL-lacZ LYS2::GAL-HIS3) containing the HIS3 and lacZ reporter genes under the control of GAL4-binding sites. The host strain containing plasmids pAS2-SNF1 and pACT2-SNF4 was used as a positive control [37]. Various negative controls that included single or dual transformants were also run in the same assay. The AH109 yeast cells were transformed using the lithium acetate procedure and plated on either complete YPD medium or synthetic dextrose (SD) in the absence of either leucine (SD/L-) or tryptophan (SD/T-), or both (SD/LT-). Protein interaction was tested by growth on SD plates without leucine, tryptophan and histidine (SD/LTH-) and the specificity of the interaction was tested as growth on SD/LTH- plates containing 20 mM 3-amino-1, 2, 3-triazole (SD/LTH-3AT). The β-galactosidase filter-lift assay was carried out as described earlier [35, 36]. A semi-quantitative liquid β-galactosidase assay was carried out using the substrate chlorophenol red-β-D-galactopyranosidase as described elsewhere [38].
In vitro expression and analysis
The in vitro expression of full-length Vpu or its TM or cytoplasmic domains was carried out using a coupled transcription-translation system (TNT; Promega, Madison, USA) as recommended by the supplier. The proteins were labeled with 35S-methionine in the same reaction and their authenticity was checked by immunoprecipitation with anti-Vpu antibodies. Five μl of the in vitro expression mix was analyzed by electrophoresis on 12% native polyacrylamide gels.
Pull-down assays
The vpu gene was cloned as a BamHI-EcoRI fragment into the pMal-c2 vector (New England Biolabs, Beverly, USA) and the maltose-binding protein-Vpu fusion (MBP-Vpu) protein was expressed in BL21(DE3) cells. Following induction of a freshly diluted overnight culture with 1 mM IPTG for 4 hr at 37°C, the cells were harvested and resuspended in PBS containing 0.1% Triton-X100, 10 μg/ml lysozyme and 1 mM PMSF, and subjected to 5 cycles each of freeze-thaw and sonication. The sample was centrifuged at 12,000 rpm at 4°C in a microfuge (Biofuge 17RS, Heraeus). To the clarified lysate, amylose resin (New England Biolabs, Beverly, USA) was added and the MBP-Vpu protein was allowed to bind for 2 hr at 4°C. The resin was washed with 10 volumes of wash buffer containing 20 mM Tris-HCl, pH 7.4, 0.2 M NaCl, 10 mM β-mercaptoethanol, 1 mM EDTA to remove non-specifically bound proteins. To 50 μl of amylose beads saturated with either MBP or MBP-Vpu, 25 μl of 35S-labeled Vpu (prepared using a coupled in vitro transcription-translation system) diluted in 500 μl PBS was added and allowed to bind for 3 hr at 4°C with end-over mixing. The beads were then centrifuged down and washed five times with 500 μl each of PBS containing 0.1% Triton X-100, resuspended in SDS dye loading buffer and subjected to SDS-PAGE and fluorography.
Gel permeation chromatography
The oligomeric forms of Vpu were also analyzed by gel permeation chromatography on Sephacryl S200HR (Pharmacia-Amersham). Following in vitro expression and 35S labeling, 50 μl of the reaction mixture was loaded on a 36 ml column pre-equilibrated in PBS. The column was run at a flow rate of 0.4 ml/min and 0.25 ml fractions were collected. The elution of Vpu was estimated by liquid scintillation counting and SDS-PAGE analysis of the peak radiolabeled fractions. For molecular size estimations, the column was calibrated with lysozyme and BSA under the same run conditions.
Confocal microscopy and FRET assays
Notes
Declarations
Acknowledgements
We thank Dr. H. Gottlinger, University of Massachusetts Medical School (USA) for the HIV-1 proviral DNA constructs and Dr. S. Mahalingam, Centre for DNA Fingerprinting and Diagnostics (India) for the p24 antibody. We also thank Dr. Malcolm Martin for pNL4-3, obtained through the NIH AIDS Research and Reference Reagent Program. This work was supported by a grant from the Department of Biotechnology, Government of India to SJ. The University Grants Commission and the Council of Scientific and Industrial Research, Government of India provided Research Fellowships to AH and SRD, respectively. An International Senior Research Fellowship of the Wellcome Trust (UK) to SJ funded the Confocal Microscopy Facility at ICGEB.
Authors’ Affiliations
References
- Freed EO, Martin MA: HIVs and their replication. In Field's Virology. Volume 2. 4th edition. Edited by: Knipe DM, Howley PM. Philadelphia, Lippincott Williams and Wilkins; 2001:1971-2041.Google Scholar
- Strebel K, Klimkait T, Martin MA: A novel gene of HIV-1, vpu, and its 16-kilodalton product. Science 1988, 241: 1221-1223. 10.1126/science.3261888PubMedView ArticleGoogle Scholar
- Bour S, Strebel K: The human immunodeficiency virus (HIV) type 2 envelope protein is a functional complement to HIV type 1Vpu that enhances particle release of heterologous retroviruses. J Virol 1996, 70: 8285-8300.PubMedPubMed CentralGoogle Scholar
- Ritter GD, Yamshchikov G, Cohen SJ, Mulligan MJ: Human immunodeficiency virus type 2 glycoprotein enhancement of particle budding: role of the cytoplasmic domain. J Virol 1996, 70: 2669-2673.PubMedPubMed CentralGoogle Scholar
- Cohen EA, Terwilliger EF, Sodroski JG, Haseltine WA: Identification of a protein encoded by the vpu gene of HIV-1. Nature 1988, 334: 532-534. 10.1038/334532a0PubMedView ArticleGoogle Scholar
- Schubert U, Henklein P, Boldyreff B, Wingender E, Strebel K, Porstmann T: The human immunodeficiency virus type 1 encoded Vpu protein is phosphorylated by casein kinase-2 (CK-2) at positions Ser52 and Ser56 within a predicted alpha-helix-turn-alpha-helix motif. J Mol Biol 1994, 236: 16-25. 10.1006/jmbi.1994.1114PubMedView ArticleGoogle Scholar
- Bour S, Strebel K: The HIV-1 Vpu protein: a multifunctional enhancer of viral particle release. Microbes Infection 2003, 5: 1029-1039. 10.1016/S1286-4579(03)00191-6PubMedView ArticleGoogle Scholar
- Bour S, Geleziunas R, Wainberg MA: The human immunodeficiency virus type 1 (HIV-1) CD4 receptor and its central role in the promotion of HIV-1 infection. Microbiol Rev 1995, 59: 63-93.PubMedPubMed CentralGoogle Scholar
- Bour S, Schubert U, Strebel K: The human immunodeficiency virus type 1 Vpu protein specifically binds to the cytoplasmic domain of CD4: implications for the mechanism of degradation. J Virol 1995, 69: 1510-1520.PubMedPubMed CentralGoogle Scholar
- Margottin F, Bour SP, Durand H, Selig L, Benichou S, Richard V, Thomas D, Strebel K: A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif. Mol Cell 1998, 1: 565-574. 10.1016/S1097-2765(00)80056-8PubMedView ArticleGoogle Scholar
- Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SW: SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 1996, 86: 263-274. 10.1016/S0092-8674(00)80098-7PubMedView ArticleGoogle Scholar
- Fujita K, Omura S, Silver J: Rapid degradation of CD4 in cells expressing human immunodeficiency virus type 1 Env and Vpu is blocked by proteasome inhibitors. J Gen Virol 1997, 78: 619-625.PubMedView ArticleGoogle Scholar
- Schubert U, Anton LC, Bacik I, Cox JH, Bour S, Bennink JR, Orlowski M, Strebel K, Yewdell JW: CD4 glycoprotein degradation induced by human immunodeficiency virus type 1 Vpu protein requires the function of proteasomes and the ubiquitin-conjugating pathway. J Virol 1998, 72: 2280-2288.PubMedPubMed CentralGoogle Scholar
- Bour S, Perrin C, Akari H, Strebel K: The human immunodeficiency virus type 1 Vpu protein inhibits NF-kappa B activation by interfering with beta TrCP-mediated degradation of Ikappa B. J Biol Chem 2001, 276: 15920-15928. 10.1074/jbc.M010533200PubMedView ArticleGoogle Scholar
- Akari H, Bour S, Kao S, Adachi A, Strebel K: The human immunodeficiency virus type 1 accessory protein Vpu induces apoptosis by suppressing the nuclear factor kappa B-dependent expression of antiapoptotic factors. J Exp Med 2001, 194: 1299-1311. 10.1084/jem.194.9.1299PubMedPubMed CentralView ArticleGoogle Scholar
- Klimkait T, Strebel K, Hoggan MD, Martin MA, Orenstein JM: The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release. J Virol 1990, 64: 621-629.PubMedPubMed CentralGoogle Scholar
- Ewart GD, Sutherland T, Gage PW, Cox GB: The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels. J Virol 1996, 70: 7108-7115.PubMedPubMed CentralGoogle Scholar
- Callahan MA, Handley MA, Lee YH, Talbot KJ, Harper JW, Panganiban AT: Functional interaction of human immunodeficiency virus type 1Vpu and Gag with a novel member of the tetratricopeptide repeat protein family. J Virol 1998, 72: 5189-5197.PubMedPubMed CentralGoogle Scholar
- Paul M, Mazumder S, Raja N, Jabbar MA: Mutational analysis of the human immunodeficiency virus type 1 Vpu transmembrane domain that promotes the enhanced release of virus-like particles from the plasma membrane of mammalian cells. J Virol 1998, 72: 1270-1279.PubMedPubMed CentralGoogle Scholar
- Schubert U, Bour S, Ferrer-Montiel AV, Montal M, Maldarelli F, Strebel K: The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains. J Virol 1996, 70: 809-819.PubMedPubMed CentralGoogle Scholar
- Hout DR, Gomez ML, Pacyniak E, Gomez LM, Inbody SH, Mulcahy ER, Culley N, Pinson DM, Powers MF, Wong SW, Stephens EB: Scrambling of the amino acids within the transmembrane domain of Vpu results in a simian-human immunodeficiency virus (SHIVTM) that is less pathogenic for pig-tailed macaques. Virology 2005, 339: 56-69. 10.1016/j.virol.2005.04.038PubMedView ArticleGoogle Scholar
- Maldarelli F, Chen MY, Willey RL, Strebel K: Human immunodeficiency virus type 1 Vpu protein is an oligomeric type I integral membrane protein. J Virol 1993, 67: 5056-5061.PubMedPubMed CentralGoogle Scholar
- Moore PB, Zhong Q, Husslein T, Klein ML: Simulation of the HIV-1 Vpu transmembrane domain as a pentameric bundle. FEBS Lett 1998, 431: 143-148. 10.1016/S0014-5793(98)00714-5PubMedView ArticleGoogle Scholar
- Xia Z, Liu Y: Reliable and Global Measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys J 2001, 81: 2395-2402.PubMedPubMed CentralView ArticleGoogle Scholar
- Siegel RM, Chan FK-M, Zacharias DA, Swofford R, Holmes KL, Tsien RY, Leonardo MJ: Measurement of molecular interactions in living cells by fluorescence resonance energy transfer between variants of the green fluorescent protein. Sci STKE 2000, 38: pl1. [DOI: 10.1126/stke.2000.38.pl1].Google Scholar
- Schubert U, Ferrer-Montiel AV, Oblatt-Montal M, Henklein P, Strebel K, Montal M: Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells. FEBS Lett 1996, 398: 12-18. 10.1016/S0014-5793(96)01146-5PubMedView ArticleGoogle Scholar
- Marassi FM, Ma C, Gratowski H, Strauss SK, Strebel K, Oblatt-Montal M, Montal M, Opella SJ: Correlation of the structural and functional domains in the mebrane protein Vpu from HIV-1. Proc Natl Acad Sci USA 1999, 86: 14336-14341. 10.1073/pnas.96.25.14336View ArticleGoogle Scholar
- Lopez CF, Montal M, Blasie JK, Klein ML, Moore PB: Molecular dynamics investigation of membrane-bound bundles of the channel-forming transmembrane domain of viral protein U from the human immunodeficiency virus HIV-1. Biophys J 2002, 83: 1259-1267.PubMedPubMed CentralView ArticleGoogle Scholar
- Becker CFW, Oblatt-Montal M, Kochendoerfer GG, Montal M: Chemical synthesis and single channel properties of tetrameric and pentameric TASPs (template-assembled synthetic proteins) derived from the transmembrane domain of HIV virus protein u (Vpu). J Biol Chem 2004, 279: 17483-17489. 10.1074/jbc.M313212200PubMedView ArticleGoogle Scholar
- Raja NU, Jabbar MA: The human immunodeficiency virus type 1 Vpu protein tethered to the CD4 extracellular domain is localized to the plasma membrane and is biologically active in the secretory pathway of mammalian cells: implications for the mechanisms of Vpu function. Virol 1996, 220: 141-151. 10.1006/viro.1996.0294View ArticleGoogle Scholar
- Pacyniak E, Gomez ML, Gomez LM, Mulcahy ER, Jackson M, Hout DR, Wisdom BR, Stephens EB: Identification of a region within the cytoplasmic domain of the subtype B Vpu protein of human immunodeficiency virus type 1 (HIV-1) that is responsible for retention in the Golgi complex and its absence in the Vpu protein from a subtype C HIV-1. AIDS Res Hum Ret 2005, 21: 379-394. 10.1089/aid.2005.21.379View ArticleGoogle Scholar
- Hengartner MO: The biochemistry of apoptosis. Nature 2000, 407: 770-776. 10.1038/35037710PubMedView ArticleGoogle Scholar
- Rizzuto R, Duchen MR, Pozzan T: Flirting in little space: the ER/mitochontria Ca 2+ liaison. Sci STKE 2004. re1.Google Scholar
- Peterlin BM, Trono D: Hide, shield and strike back: how HIV-infected cells avoid immune eradication. Nat Rev Immunol 2003, 3: 97-107. 10.1038/nri998PubMedView ArticleGoogle Scholar
- Kar-Roy A, Korkaya H, Oberoi R, Lal SK, Jameel S: The hepatitis E virus open reading frame 3 protein activates ERK through binding and inhibition of the MAPK phosphatase. J Biol Chem 2004, 279: 28345-28357. 10.1074/jbc.M400457200PubMedPubMed CentralView ArticleGoogle Scholar
- Tyagi S, Surjit M, Kar-Roy A, Jameel S, Lal SK: The ORF3 protein of hepatitis E virus interacts with liver-specific α 1 -microglobulin and its precursor, α 1 -microglobulin.bikunin precursor (AMBP) and expedites their export from the hepatocyte. J Biol Chem 2004, 279: 29308-29319. 10.1074/jbc.M402017200PubMedView ArticleGoogle Scholar
- Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ: The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 1993, 75: 805-816. 10.1016/0092-8674(93)90499-GPubMedView ArticleGoogle Scholar
- Bai C, Elledge SJ: Gene identification using the yeast two-hybrid system. Methods Enzymol 1996, 273: 331-347.PubMedView ArticleGoogle Scholar
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