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Figure 4 | Virology Journal

Figure 4

From: Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis

Figure 4

Gel permeation analysis of Vpu oligomers. In vitro translated and 35S-labeled Vpu proteins were separated by gel permeation chromatography as described in Methods. The eluted 35S counts in each fraction are indicated for the full-length (black circles) or TM domain (black squares) Vpu proteins. The positions of Vpu monomers and oligomers are indicated. The inset shows SDS-PAGE analysis of the peak fractions. Lanes: 1, Vpu oligomer; 2, Vpu monomer; 3, TM domain oligomer; 4, TM domain monomer; lane M shows molecular size marker as indicated (in kilodaltons).

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Virology Journal

ISSN: 1743-422X