- Open Access
The effects of hepatitis C virus core protein on the expression of miR-122 in vitro
© Li et al.; licensee BioMed Central Ltd. 2013
- Received: 5 March 2012
- Accepted: 13 March 2013
- Published: 27 March 2013
Hepatitis C virus (HCV) is one of the major pathogens of liver diseases. Some studies have previously reported that miR-122 can stimulate replication or translation of HCV. However, the effects of HCV infection on miR-122 expression are not clear. The aim of this study was to investigate the effects of HCV core protein on the expression of miR-122 in a cell culture model.
The miR-122 levels in Huh7.5.1 cells infected with HCV for different days or different HCV abundance were measured by real-time PCR. Significant decrease of miR-122 expression was found at late stage of infection and in the high-abundance group. Huh7.5.1 cells transfected with plasmid pEGFP-core or pEGFP were used to detect the effects of HCV core protein on miR-122 expression, the results showed that core protein could down-regulate the miR-122 expression level in a time- and dose- dependent manner, and reduced the susceptibility of Huh7.5.1 cell to HCV.
Down-regulating miR-122 expression by HCV core protein may give a new insight into the interaction between HCV and miR-122 and chronic HCV infection.
Hepatitis C virus (HCV) is one of the major causes of viral hepatitis and hepatocellular carcinoma, which infects an estimated 170 million people throughout the world [1, 2]. Currently, pegylated interferon-α (IFN-α) combined with ribavirin is the standard of care for chronic HCV infection. The therapy has a sustained virological response rate of 40% ~ 50% in patients with genotype 1 HCV infection [3–5], which accounts for the majority of the hepatitis C patient population in the developed countries. A range of non-structural protease inhibitors are developed to act as antiviral agents against HCV, such as boceprevir [6, 7] and telaprevir [8, 9]. However, genetic heterogeneity between HCV genotypes influences the structure of protease and limits the effectiveness of the antivirals . Most people infected with HCV develop chronic liver disease . The success of HCV in persisting is linked to its overall ability to evade antiviral defences. Several HCV structural and nonstructural proteins, including Core [12, 13], E2 , NS3/4A [15, 16] and NS5A  proteins, have been shown to inhibit the innate immune response.
miRNAs are post-transcriptional regulators that bind to complementary sequences on target messenger RNA transcripts (mRNAs), usually resulting in translational repression or target degradation and gene silencing. miR-122, a liver-specific miRNA expressed at high levels in hepatocytes, constitutes 70% of the total miRNA in the liver . miR-122 enhanced the replication of HCV through a direct interaction with the 5’noncoding region (NCR) of the HCV genome . Henke et al. showed that miR-122 stimulated translation of HCV by enhancing the association of ribosomes with the viral RNA . Furthermore, it was proved that up-regulation of HMOX1, which was the target gene of miR-122, decreased HCV RNA . However, the effects of HCV on the expression of miR-122 haven’t been fully explored yet.
In the current study, a cell culture model based on Jc1 clone and Huh7.5.1 cell was used to investigate the effects of HCV on the expression of miR-122. Data showed that HCV decreased the expression of miR-122 in a time- and dose- dependent manner. Further results revealed that the HCV core protein performed this function. These results give a novel insight into the interaction between HCV and miR-122.
HCV decreased the expression of miR-122 in a time- and dose- dependent manner
HCV core protein down-regulated miR-122 expression
HCV core protein down-regulated susceptibility of Huh7.5.1 cells to HCV
It is generally believed that miR-122, specifically expressed and highly abundant in the liver, facilitates replication of HCV. That is the reason why HCV RNA can only replicate in Huh7 cells, but not in HepG2 cells, which do not express endogenous miR-122 .
In recent years, many studies have focused on the relationship between HCV and miR-122. There is, however, only limited information about the role of HCV in miR-122 expression. In our study, the intracellular levels of HCV RNA and miR-122 were measured at different time points after infecting with HCV. The results indicated that the level of HCV RNA increased rapidly during the infection, and there was a positive correlation between miR-122 level and HCV RNA level at the early stage of infection to some extent. However, in previous reports, no positive correlation was observed between miR-122 level and HCV [22, 23]. It may be due to not strong correlation between miR-122 level and HCV RNA level or the different cell line. It was interesting that at day 19 after infection with HCV, there was an inverse correlation between miR-122 expression and HCV RNA expression, and miR-122 expression level was obviously reduced at the late stage of HCV infection. This inverse correlation between HCV and miR-122 was consistent with a previous in vitro study, which reported that miR-122 levels were significantly reduced on HCVcc infection . It was also found in our study that miR-122 expression level decreased in high-abundance HCV group. In a vivo experiment, a similar result was observed that the HCV subjects with higher viral load had lower miR-122 levels in liver than HCV subjects with lower viral load . Another similar result was published that hepatic miR-122 expression in serum HCV RNA-positive patients was significantly lower than that in serum HCV RNA-negative patients . Thus, it seemed that, with the accumulation of HCV, the inhibitory effect of HCV on miR-122 might be observed. Taken together, these results might support the notion that HCV monitors its replication by down-regulating the miR-122 level. Actually, a recently proposed hypothesis may provide a similar mechanistic explanation that, in order to survive, HCV need a steady viral replication rate [27–30]. By keeping abundance relatively low, the virus evades the immune response of the host and establishes persistent chronic infection.
The subsequent results demonstrated that it was HCV core protein that suppressed miR-122 expression both in a time- and dose- dependent manner. And overexpression of HCV core protein did reduce the susceptibility of Huh7.5.1 cells to HCV, suggesting it might participate in the mechanism for self-regulation of HCV, which might be important for viral persistence. However, further studies are needed to address the precise mechanism by which core protein functions.
That HCV core protein suppresses miR-122 expression may be involved in the mechanism of evading the immune response of the host.
HCV plasmid pFL-Jc1 was provided by Apath (Saint Louis, Missouri, USA). The plasmid pEGFP-core expressing GFP-tagged HCV core protein and pEGFP were constructed in our laboratory.
The human hepatoma cell line Huh7.5.1 was kindly provided by Scott Forrest (Scripps Research Institute, La Jolla, CA, USA) and Jin Zhong (Institute Pasteur of Shanghai, China). Huh7.5.1 cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml of penicillin, 100 μg/ml of streptomycin and 2 mM L-glutamine, at 37°C with 5.0% CO2.
HCV Jc1 virus preparation and infection
In brief, the plasmid pFL-Jc1 was linearized by XbaI (New England Biolabs). Then HCV RNA was synthesized from the linearized DNA template with T7 RiboMAX™ Express Large Scale RNA Production System (Promega). Huh7.5.1 cells were regularly passaged and plated in 12-well culture plates for 20 h before transfection at 60% confluency. In vitro transcribed HCV RNA was delivered into Huh7.5.1 cells by Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions. Transfected cells were passaged every 2–3 days before the cells became confluent. The supernatant was collected at day 3 or day 9 posttransfection and used as infectious HCV particles.
Naïve Huh7.5.1 cells plated in 12-well plate at a density of 4 × 104 cells per well were incubated with culture medium containing virus (50 IU/cell) for 4 h at 37°C. Then the inoculum was discarded and fresh medium was added. Cells were passaged every 2–3 days before the cells became confluent. For each passage, half of the cell culture supernatant was discarded and fresh medium was added.
Huh7.5.1 cells were regularly passaged and plated in 12-well culture plates for 20 h before transfection at 60% confluency. Plasmids pEGFP-core or pEGFP were delivered into Huh7.5.1 cells by Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions.
Real-time PCR detection
HCV RNA in culture supernatant was quantified using diagnostic kit for quantification of hepatitis C virus RNA (Shanghai KeHua Bio-Engineering Co., Ltd.), according to the protocol. Tenfold serial dilutions of in vitro-transcribed HCV RNAs (from 104-107 IU/ml) served as standards, which were included in the Kit. In brief, HCV RNA in 100 μl culture supernatant was extracted using nucleic acid extraction columns included in the kit. The 12.5 μl of extracted RNA was amplified in each reaction well containing 7 μl of PCR major reaction solution, 5 μl of enzyme mixture and 0.5 μl of probe. The temperature profile comprised the RT round conducted at 50°C for 25 min and the PCR rounds denaturing at 94°C for 2 min and then at 94°C for 10 s, 55°C for 15 s and 72°C for 15 s, and completed by a 42-cycle program at 94°C for 10 s and 60°C for 45 s. Real-time PCR amplification was performed using the ABI 7500 system (Applied Biosystems).
Cellular RNA was extracted using Trizol (Invitrogen). For HCV RNA and GAPDH detection, RNA was reverse transcribed using the PrimeScript RT reagent Kit (Takara) and the cDNA production was quantified by Real-time PCR with SYBR Premix Ex Taq (Takara) according to the manufacturer’s protocol. The primers sequence for HCV were: forward primer: GCGTTAGTATGAGTGTCGTG and reverse primer: TCGCAAGCACCCTATCAG and primers sequence for GAPDH were: forward primer: GAAGGTGAAGGTCGAGTC and reverse primer: GAAGATGGTGATGGGATTTC. For miRNA detection, RT and real-time PCR were performed using miRNA analysis kits (Applied Biosystems). The stem-loop RT primers and real-time primers for miR-122 and U6 were synthesized by Ribobio (Guangzhou, China).
Huh7.5.1 cells infected with HCV were seeded on coverslips in 12-well, then fixed in 4% paraformaldehyde for 10 min at −20°C. Fixed cells were permeabilized in 0.2% Triton-X100/PBS for 10 min at room temperature and blocked in a solution of 2% BSA/PBS for 30 min at room temperature. Then cells were incubated with anti-HCV Core antibody (Abcam) diluted 1:500, at 37°C for 2 h, followed by anti-mouse IgG conjugated to Alexa 488 (Sigma) diluted 1:500, at 37°C for 1 h. Coverslips were mounted on slides with 50% glycerol/PBS. Then, cells were examined by a Zeiss LSM 510 laser scanning confocal microscope.
Data were given as mean ± SD obtained from three separate experiments. Statistical analysis was performed by using one-way ANOVA or by independent-sample Student’s t-test, using SPSS ver. 11.5 software. P-values < 0.05 were considered statistically significant.
We would like to thank Scott Forrest and Jin Zhong for providing us with Huh7.5.1 cells and Apath for providing us with the pFL-Jc1 plasmid. This work was supported by Science and Technology Major Projects of Zhejiang Province (2009C03011-2) and National Science and Technology Major project (2012ZX10002-003).
- Lauer GM, Walker BD: Hepatitis C virus infection. N Engl J Med 2001,345(1):41-52. 10.1056/NEJM200107053450107PubMedView ArticleGoogle Scholar
- Rehermann B, Nascimbeni M: Immunology of hepatitis B virus and hepatitis C virus infection. Nat Rev Immunol 2005,5(3):215-229. 10.1038/nri1573PubMedView ArticleGoogle Scholar
- Fried MW, Shiffman ML, Reddy KR, Smith C, Marinos G, Goncales FL Jr, Häussinger D, Diago M, Carosi G, Dhumeaux D, Craxi A, Lin A, Hoffman J, Yu J: Peginterferon alfa-2a plus ribavirin for chronic hepatitis c virus infection. N Engl J Med 2002,347(13):975-982. 10.1056/NEJMoa020047PubMedView ArticleGoogle Scholar
- Hadziyannis SJ, Sette H Jr, Morgan TR, Balan V, Diago M, Marcellin P, Ramadori G, Bodenheimer H Jr, Bernstein D, Rizzetto M, Zeuzem S, Pockros PJ, Lin A, Ackrill AM, PEGASYS International Study Group: Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose. Ann Intern Med 2004,140(5):346-355.PubMedView ArticleGoogle Scholar
- Manns MP, McHutchison JG, Gordon SC, Rustgi VK, Shiffman M, Reindollar R, Goodman ZD, Koury K, Ling M, Albrecht JK: Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet 2001,358(9286):958-965. 10.1016/S0140-6736(01)06102-5PubMedView ArticleGoogle Scholar
- Poordad F, McCone J Jr, Bacon BR, Bruno S, Manns MP, Sulkowski MS, Jacobson IM, Reddy KR, Goodman ZD, Boparai N, DiNubile MJ, Sniukiene V, Brass CA, Albrecht JK, Bronowicki JP, SPRINT-2 Investigators: Boceprevir for untreated chronic HCV genotype 1 infection. N Engl J Med 2011,364(13):1195-1206. 10.1056/NEJMoa1010494PubMedPubMed CentralView ArticleGoogle Scholar
- Bacon BR, Gordon SC, Lawitz E, Marcellin P, Vierling JM, Zeuzem S, Poordad F, Goodman ZD, Sings HL, Boparai N, Burroughs M, Brass CA, Albrecht JK, Esteban R, HCV RESPOND-2 Investigators: Boceprevir for previously treated chronic HCV genotype 1 infection. N Engl J Med 2011,364(13):1207-1217. 10.1056/NEJMoa1009482PubMedPubMed CentralView ArticleGoogle Scholar
- Jacobson IM, McHutchison JG, Dscheiko G, Di Bisceglie AM, Reddy KR, Bzowej NH, Marcellin P, Muir AJ, Ferenci P, Flisiak R, George J, Rizzeto M, Shouval D, Sola R, Terg RA, Yoshida EM, Adda N, Bengtsson L, Sankoh AJ, Kieffer TL, George S, Kauffman RS, Zeuzem S, ADVANCE Study Team: Telaprevir for previously untreated chronic hepatitis C virus infetion. N Engl J Med 2011,364(25):2405-2416. 10.1056/NEJMoa1012912PubMedView ArticleGoogle Scholar
- Zeuzem S, Andreone P, Pol S, Lawitz E, Diago M, Roberts S, Focaccia R, Younossi Z, Foster GR, Hrban A, Ferenci P, Nevens F, Müllhaupt B, Pockros P, Terg R, Shouval D, Van Hoek B, Weiland O, Van Heeswijk R, De Meyer S, Luo D, Boogaerts G, Polo R, Picchio G, Beumont M, REALIZE Study Team: Telaprevir for retreatment of HCV infetion. N Engl J Med 2011,364(25):2417-2428. 10.1056/NEJMoa1013086PubMedView ArticleGoogle Scholar
- Inhof I, Simmonds P: Genotype differences in susceptibility and resistance development of hepatitis C virus to protease inhibitors telaprevir (VX-950) and danoprevir (ITMN-191). Hepatology 2011,53(4):1090-1099. 10.1002/hep.24172View ArticleGoogle Scholar
- Flamm SL: Chronic hepatitis C virus infection. JAMA 2003,289(18):2413-2417. 10.1001/jama.289.18.2413PubMedView ArticleGoogle Scholar
- Melen K, Fagerlund R, Nyqvist M, Keskinen P, Julkunen I: Expression of hepatitis C virus core protein inhibits interferon-induced nuclear import of STATs. J Med Virol 2004,73(4):536-547. 10.1002/jmv.20123PubMedView ArticleGoogle Scholar
- Miller K, McArdle S, Gale MJ Jr, Geller DA, Tenoever B, Hiscott J, Gretch DR, Polyak SJ: Effects of the hepatitis C virus core protein on innate cellular defense pathways. J Interferon Cytokine Res 2004,24(7):391-402. 10.1089/1079990041535647PubMedView ArticleGoogle Scholar
- Taylor DR, Shi ST, Romano PR, Barber GN, Lai MM: Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science 1999,285(5424):107-110. 10.1126/science.285.5424.107PubMedView ArticleGoogle Scholar
- Li K, Foy E, Ferreon JC, Nakamura M, Ferreon AC, Ikeda M, Ray SC, Gale M Jr, Lemon SM: Immune evasion by hepatitis C virus NS3/4A protease-mediated cleavage of the Toll-like receptor 3 adaptor protein TRIF. Proc Natl Acad Sci USA 2005,102(8):2992-2997. 10.1073/pnas.0408824102PubMedPubMed CentralView ArticleGoogle Scholar
- Li XD, Sun L, Seth RB, Pineda G, Chen ZJ: Hepatitis C virus protease NS3/4A mitochondrial antiviral signaling protein off the mitochondria to evade innate immunity. Proc Natl Acad Sci USA 2005,102(49):17717-17722. 10.1073/pnas.0508531102PubMedPubMed CentralView ArticleGoogle Scholar
- Song J, Fujii M, Wang F, Itoh M, Hotta H: The NS5A protein of hepatitis C virus partially inhibits the antiviral activity of interferon. J Gen Virol 1999,80(Pt 4):879-886.PubMedView ArticleGoogle Scholar
- Chang J, Nicolas E, Marks D, Sander C, Lerro A, Buendia MA, Xu C, Mason WS, Moloshok T, Bort R, Zaret KS, Taylor JM: miR-122, a mammalian liver-specific microRNA, is processed from hcr mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1. RNA Biol 2004,1(2):106-113. 10.4161/rna.1.2.1066PubMedView ArticleGoogle Scholar
- Jopling CL, Yi M, Lancaster AM, Lemon SM, Sarnow P: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science 2005,309(5740):1577-1581. 10.1126/science.1113329PubMedView ArticleGoogle Scholar
- Henke JI, Goergen D, Zheng J, Song Y, Schϋttler CG, Fehr C, Jϋnemann C, Niepmann M: microRNA-122 stimulates translation of hepatitis C virus RNA. EMBO J 2008,27(24):3300-3310. 10.1038/emboj.2008.244PubMedPubMed CentralView ArticleGoogle Scholar
- Shan Y, Zheng J, Lambrecht RW, Bonkovsky HL: Reciprocal effects of micro-RNA-122 on expression of heme oxygenase-1 and hepatitis C virus genes in human hepatocytes. Gastroenterology 2007,133(4):1166-1174. 10.1053/j.gastro.2007.08.002PubMedPubMed CentralView ArticleGoogle Scholar
- Cermelli S, Ruggieri A, Marrero JA, Ioannou GN, Beretta L: Circulating microRNAs in patients with chronic hepatitis C and non-alcoholic fatty liver disease. PLoS One 2011,6(8):e23937. 10.1371/journal.pone.0023937PubMedPubMed CentralView ArticleGoogle Scholar
- Randall G, Panis M, Cooper JD, Tellinghuisen TL, Sukhodolets KE, Pfeffer S, Landthaler M, Landgraf P, Kan S, Lindenbach BD, Chien M, Weir DB, Russo JJ, Ju J, Brownstein MJ, Sheridan R, Sander C, Zavolan M, Tuschl T, Rice CM: Cellular cofactors affecting hepatitis C virus infection and replication. Proc Natl Acad Sci USA 2007,104(31):12884-12889. 10.1073/pnas.0704894104PubMedPubMed CentralView ArticleGoogle Scholar
- Marquez RT, Bandyopadhyay S, Wendlandt EB, Keck K, Hoffer BA, Icardi MS, Christensen RN, Schmidt WN, McCaffrey AP: Correlation between microRNA expression levels and clinical parameters associated with chronic hepatitis C viral infection in humans. Lab Invest 2010,90(12):1727-1736. 10.1038/labinvest.2010.126PubMedView ArticleGoogle Scholar
- Sarasin-Filipowicz M, Krol J, Markiewicz I, Heim MH, Filipowicz W: Decreased levels of microRNA miR-122 in individuals with hepatitis C responding poorly to interferon therapy. Nat Med 2009,15(1):31-33. 10.1038/nm.1902PubMedView ArticleGoogle Scholar
- Morita K, Taketomi A, Shirabe K, Umeda K, Kayashima H, Ninomiya M, Uchiyama H, Soejima Y, Maehara Y: Clinical significance and potential of hepatic microRNA-122 expression in hepatitis C. Liver Int 2011,31(4):474-484. 10.1111/j.1478-3231.2010.02433.xPubMedView ArticleGoogle Scholar
- Sallie R: Replicative homeostasis: a mechanism of viral persistence. Med Hypotheses 2004,63(3):515-523. 10.1016/j.mehy.2004.02.050PubMedView ArticleGoogle Scholar
- Sallie R: Replicative homeostasis: a fundamental mechanism mediating selective viral replication and escape mutation. Virol J 2005, 2: 10. 10.1186/1743-422X-2-10PubMedPubMed CentralView ArticleGoogle Scholar
- Sallie R: Replicative homeostasis II: influence of polymerase fidelity on RNA virus quasispecies biology: implications for immune recognition, viral autoimmunity and other "virus receptor" diseases. Virol J 2005, 2: 70. 10.1186/1743-422X-2-70PubMedPubMed CentralView ArticleGoogle Scholar
- Sallie R: Replicative homeostasis III: implications for antiviral therapy and mechanisms of response and non-response. Virol J 2007, 4: 29. 10.1186/1743-422X-4-29PubMedPubMed CentralView ArticleGoogle Scholar
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