Fig. 1
From: HBx inhibits DNA sensing signaling pathway via ubiquitination and autophagy of cGAS
Hepatitis B virus X protein is a negative regulator of cGAS-Induced IFN-I-β signaling. (a–c) HEK293T cells were transfected with IFN-I-β luciferase reporter and expression plasmids as indicated, and luciferase activity was assayed 24 h after transfection. The relative luciferase activity was expressed as arbitrary units by normalizing firefly luciferase activity to Renilla luciferase activity. (d) HEK293T cells were transfected with expression plasmids as indicated, at 24 h posttransfection, cells were harvested and subjected to qRT-PCR analysis. (e) Western blot (WB) analysis of cGAS, STING in several human hepatic cell lines. (f, g) HEK293T cells were cotransfected with cGAS and STING, along with empty vector or HBx-Flag plasmid, Western blot analysis of IRF3 dimerization, and phosphorylation