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Fig. 5 | Virology Journal

Fig. 5

From: Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI

Fig. 5

Levels of IgG to p30 and p54 detected by indirect ELISAs. Five BALB/c mice/group were immunized by subcutaneous route with each recombinant protein (30 μg) or an equal volume of PBS. Blood samples were obtained at 0, 7, 21 and 28 dpv. IgG levels to p30 (A) and p54 (B) of each group at serum dilutions of 1:100 were measured by indirect ELISAs and are expressed as OD450 (mean ± SD, n = 5). Characterization of p30-specific (C) and p54-specific (D) IgG isotype profiles of sera (diluted in twofold series) from immunized mice at 28 dpv by indirect ELISAs. The titer of a given serum sample is defined as the reciprocal value of the highest dilution yielding a positive result and displayed as mean ± SD (n = 3); ns = P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001

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Virology Journal

ISSN: 1743-422X