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Table 1 Primers used for amplification of sequencing fragments

From: Sequence and phylogentic analysis of MERS-CoV in Saudi Arabia, 2012–2019

Amplification fragment Target gene Primer name, position* and sense Primer sequence (5′–3′) Expected size (bp) References
1 ORF1a MERS-3047 (+) ACT GCG TGG AAT GCC GAT TC 843 This study
MERS-3890 (−) GCC TAC GAC ATG CAG GAT ATT C
2 ORF1a MERS-8089 (+) CAA CAT TCA TTG ACG CAG CAC 946 [35]
MERS-9034 (−) GGA TCA TGG CAG TAT GGT GTC
3 ORF1a MERS-11419 (+) CAA GCC CCA TTG CCT ATC TG 646 [34]
MERS-12064 (−) GCT TGA AGT ACG CTA GGA GTG
4 ORF1b MERS-18791 (+) CAT CAA GGA GCT CAT GTG GC 792 [35]
MERS-19582 (−) TTC CAA ACC TTG AAC TTT TGT AAA AG
5 Spike MERS-21425 (+) CTG TCG CAG GGT AAG TTA CTT ATC 756 This study
MERS-22180 (−) GTG TAC ATA AAG GTG CAG TTA CG
6 Spike MERS-22116 (+) CGT AAT GCC AGT CTG AAC TC 918 [34]
MERS-23032 (−) CAG GGT GAG TAT TGA TTA GCG
7 Spike MERS-22990 (+) CTG AAG TAC CTC AGT TAG TGA ACG 1225 This study
MERS-24214 (−) GCT GAT GCT GGA CCT TGC TG
8 ORF3/4a MERS-25465 (+) GTG ATA GAT ACG AGG AAT ACG ACC 780 This study
MERS-26001 (−) GGA TAG CTG ACA GTT CCA CAG
9 ORF4b MERS-26042 (+) CTT TGG CCA AAC AGG ACG CA 536 [34]
MERS-26856 (−) GAC GCC GAG AAA GCC ATA GTT C
  1. ORF = open reading frame, MERS = Middle East Respiratory Syndrome, bp = base pair
  2. *Positions are relevant to the complete genome sequence of Human betacoronavirus 2c EMC/2012 (GenBank accession number JX869059)