Table 1 Primers used for amplification of sequencing fragments
From: Sequence and phylogentic analysis of MERS-CoV in Saudi Arabia, 2012–2019
Amplification fragment | Target gene | Primer name, position* and sense | Primer sequence (5′–3′) | Expected size (bp) | References |
|---|---|---|---|---|---|
1 | ORF1a | MERS-3047 (+) | ACT GCG TGG AAT GCC GAT TC | 843 | This study |
MERS-3890 (−) | GCC TAC GAC ATG CAG GAT ATT C | ||||
2 | ORF1a | MERS-8089 (+) | CAA CAT TCA TTG ACG CAG CAC | 946 | [35] |
MERS-9034 (−) | GGA TCA TGG CAG TAT GGT GTC | ||||
3 | ORF1a | MERS-11419 (+) | CAA GCC CCA TTG CCT ATC TG | 646 | [34] |
MERS-12064 (−) | GCT TGA AGT ACG CTA GGA GTG | ||||
4 | ORF1b | MERS-18791 (+) | CAT CAA GGA GCT CAT GTG GC | 792 | [35] |
MERS-19582 (−) | TTC CAA ACC TTG AAC TTT TGT AAA AG | ||||
5 | Spike | MERS-21425 (+) | CTG TCG CAG GGT AAG TTA CTT ATC | 756 | This study |
MERS-22180 (−) | GTG TAC ATA AAG GTG CAG TTA CG | ||||
6 | Spike | MERS-22116 (+) | CGT AAT GCC AGT CTG AAC TC | 918 | [34] |
MERS-23032 (−) | CAG GGT GAG TAT TGA TTA GCG | ||||
7 | Spike | MERS-22990 (+) | CTG AAG TAC CTC AGT TAG TGA ACG | 1225 | This study |
MERS-24214 (−) | GCT GAT GCT GGA CCT TGC TG | ||||
8 | ORF3/4a | MERS-25465 (+) | GTG ATA GAT ACG AGG AAT ACG ACC | 780 | This study |
MERS-26001 (−) | GGA TAG CTG ACA GTT CCA CAG | ||||
9 | ORF4b | MERS-26042 (+) | CTT TGG CCA AAC AGG ACG CA | 536 | [34] |
MERS-26856 (−) | GAC GCC GAG AAA GCC ATA GTT C |