Fig. 2

Deduced amino acid alignments of representative strains from different years were selected, and the alignment was done by Clustal W method running within the MegAlign program (DNAstar). Alignments are shown in comparison with the consensus sequences of the first isolated MERS-CoV strain (EMC-2012). Dots represent the identical amino acid residues. a displays 80 deduced amino acids of the S protein RBD. Host and year specific amino acids are shown in boxes. b 90 amino acid residues of ORF1a/b (residues 1025 to 1105) showed a distinct pattern where several amino acids had been changed permanently in comparison with the isolate (EMC-2012); 1000 T/V, 1055 P/S, 1070 A/E, 1094 N/S