Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9

Table 1 Summary of downregulated proteins in A549 cells infected with influenza A H5N1 virus compared to H9N2-infected cells at 24 hpi (r > 2, p < 0.05)

Spot no.^a	Accession No.^b	Gene	Protein name	MW (Da)	pI	Score^c
1	P04264	KRT1	Keratin, type II cytoskeletal 1	66,170	8.15	120
2	P10809	HSPD1	60-kDa heat shock protein, mitochondrial	61,187	5.7	69
3	B4DEF7	N /A	cDNA FLJ60062, highly similar to 78-kDa glucose-regulated protein	30,458	5.77	68
4	Q59FC6	N /A	Tumor rejection antigen (Gp96) 1 variant	66,140	5.08	46
5	V9HWE1	HEL113	Epididymis luminal protein 113	53,676	5.24	156
6	Q7L4M3	KRT 8	KRT8 protein	30,802	5.05	57
7	P07237	P4HB	Protein disulfide-isomerase	57,146	5.96	60
8^*	P06576	ATP5F1B	ATP synthase subunit beta, mitochondrial	56,525	5.26	130
9	Q9UJZ1	STOML2	Stomatin-like protein 2, mitochondrial	38,839	4.45	120
10	P35908	KRT2	Keratin, type II cytoskeletal 2 epidermal	65,678	8.07	47
11	P13645	KRT10	Keratin, type I cytoskeletal 10	58,994	5.13	49
12	A8K401	PHB	Prohibitin, isoform CRA_a	29,843	4.14	175
13	P30044	PRDX5	Peroxiredoxin-5, mitochondrial	17,611	9.02	50
14	Q9BYX7	POTEKP	Putative beta-actin-like protein 3	42,331	5.91	53
15^*	Q6FHZ0	MDH2	Malate dehydrogenase, mitochondrial	35,965	8.92	63
16	P38646	HSPA9	Stress-70 protein, mitochondrial	73,967	6.03	52

^aSpot No. is the unique sample spot protein number
^bAccession No is the MASCOT result of MALDI-TOF/TOF searched from the NCBI database
^cProtein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification
^*Indicating that the differential proteins were validated by western blot analysis

ISSN: 1743-422X