Fig. 3

Infection with street RABV strains induces the phosphorylation of p38, JNK, and ERK in dog, human, and mouse brain tissues. a Representative graphs of IHC analysis of p-p38, p-JNK, and p-ERK in sections of dog and human brains naturally infected with the street RABV strains CGS-17 and CXZ-15. Uninfected dog brains served as the control group. The quantitative analyses of p-p38, p-JNK, and p-ERK signal densities are shown on the bottom. ➔ indicates positive staining cells. b Representative graphs of IHC analysis of p-p38, p-JNK, and p-ERK in sections of mouse brain tissue infected with CGS-17 and CXZ-15. Scale bars represent 50 μm. Uninfected mouse brains served as the control group. The quantitative analysis results for the p-p38, p-JNK, and p-ERK signal densities are shown on the bottom. ➔ indicates positive staining cells. c Western blot analysis of mouse brain tissue infected with CGS-17 and CXZ-15. Blots were stained for total p38, total JNK, total ERK, p-p38, p-JNK, p-ERK, and β-actin. The quantitative analysis results for the relative signal densities of total p38, total JNK, total ERK, p-p38, p-JNK, and p-ERK after normalization to the signal density of β-actin are shown on the right. Each test was performed in triplicate. Graphical data represent the mean ± SD. Statistical significance was assessed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001