Fig. 4
miR-30a-5p overexpression reduces DEV replication in DEF cells. a DEF cells were pretreated with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV for 36 h. Apoptosis was analyzed by Annexin V-FITC/PI staining using flow cytometry. b Changes in DEF viability following treatment with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then cultured in DMEM at 37 °C. The cell viability were tested by MTT assay kit at 36 h. c DEF cells were pretreated with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV. Viral copies were determined at the indicated time points. d DEF cells were pretreated with control (without), mimic-NC, miR-30a-5p mimic, inhibitor-NC and miR-30a-5p inhibitor for 4 h and then infected with DEV, and viral yields (TCID50 per 0.1 ml) were determined at the indicated time points. The data are presented as the mean ± SD for three independent experiments. *p < 0.05; **p < 0.01, compared with the control group. “ # ” indicates no significant difference, P > 0.05