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Characteristics of human papillomaviruses distribution in Guizhou Province, China

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Abstract

Background

Human papillomavirus (HPV) is one of the most common sexually transmitted viruses. Data about HPV infection in Guizhou is limited.

Methods

56,768 cervical samples were collected and genotyped for 15 main high risk and 6 main low risk HPV types.

Results

16.95% (9623/56768) of samples were HPV positive; 90.70% (8728/9623) of HPV positive women were infected by high risk HPV. High risk and high risk mix infection (1458; 70.85%) was the most common mix HPV infection type. The highest HPV detection rate was found in age group 41–45 years old (detection rate = 17.89%) (χ2 = 204.77; P < 0.001); the highest within-group HPV infection rates were found in the ≤20 (25.62%) and ≥ 61 (24.67%) years old age groups, the lowest within-group HPV infection rate was found in the 31–35 years old age group (15.02%). The highest mix infection proportions were found in the ≥61 (36.06%) and ≤ 20 (33.63%) years old age groups (χ2 = 111.21; P < 0.001), the lowest mix infection proportion was found in the 41–45 (17.42%) years old age group. The highest high risk infection proportions were found in the 26–30 (92.98%), ≥61 (92.68%), and 36–40 (92.16%) years old age groups (χ2 = 31.72; P < 0.001), the lowest high risk infection proportion was found in the ≤20 (84.96%) years old age group. HPV infection rates varied with seasons in Guizhou.

Conclusions

Characteristics of HPV distribution in Guizhou were identified. There were significant differences in HPV distribution among age groups, prevention strategies should be adjusted according to the characteristics.

Introduction

Human papillomavirus (HPV) is associated with varied epithelial lesions, including cervical intraepithelial neoplasia and genital warts [1]. Cervical cancer is one of the most common malignant tumors among women worldwide, genital warts is also very prevalent among women; each year about 500,000 new cases of cervical cancer and 1 million new cases of genital warts were diagnosed worldwide [2, 3].

More than 200 HPV types have been identified until now (pave.niaid.nih.gov). “High-risk” and “low-risk” were used to distinguish different HPV types according to their risks in causing malignant tumors. HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 83 are common high risk types; HPV 6, 11, 40, 42, 43, 44, 54, 61, 70, 72, and 81 are common low risk types [4].

HPV distribution varies geographically considerably. Effective HPV prevention and treatment strategies should be specialized based on local HPV distribution characteristics [5]. There is little data about HPV distribution characteristics in Guizhou, China. Guizhou is a province with 36 million people (data from National Bureau of Statistics of China, http://www.stats.gov.cn/). This study aimed to investigate HPV distribution characteristics in Guizhou, China, to afford data for HPV prevention and treatment locally.

Methods and materials

From January 1, 2016 to December 31, 2017, cervical swabs were obtained from patients at the Affiliated Hospital of Zunyi Medical University. Women over 18 years old with visible cervical lesions and/or HPV-related diseases (e.g. cervical intraepithelial neoplasia) were eligible for inclusion. The present study was approved by the Affiliated Hospital of Zunyi Medical University Ethics Committee, the approval number was ZYFYLS2018(81). Before sample collection, informed consent was obtained.

Cervical specimens were collected by cervical brush and stored in preservative buffer solution (NaCl 9 g, sodium benzoate 10 g, H2O 1 L) at − 20 °C. Cervical specimens were detected for HPV Genotyping by a Kit (Human Papillomavirus Genotyping Kit; Yaneng Bioscience, Shenzhen, China) according to the instruction. Positive and negative controls were included in all experiments.

SPSS version 19 (IBM, Armonk, NY, USA) was used for data analysis. The Pearson χ2 test was used to confirm the results. P < 0.05 was considered statistically significant.

Results

In total, 56,768 samples (mean age 39.45 ± 9.88 years old) were collected and detected for HPV genotyping, 16.95% (9623/56768) of samples were HPV positive; 90.70% (8728/9623) of HPV positive patients were infected by high risk HPV. HPV detection number was 12,378, 87.66% (10,850/12378) were high-risk HPV types. Detection number and detection rate of high-risk types were HPV52 (2206; 17.82%), HPV16 (1713; 13.84%), HPV58 (1435; 11.59%), HPV53 (986; 7.97%), HPV39 (923; 7.46%), HPV51 (723; 5.84%), HPV18 (534; 4.31%), HPV33 (523; 4.23%), HPV68 (456; 3.68%), HPV31 (380; 3.07%), HPV66 (309; 2.50%), HPV56 (238; 1.92%), HPV59 (210; 1.70%), HPV45 (110; 0.89%), and HPV35 (104; 0.84%). Detection number and detection rate of low-risk types were HPV81 (769; 6.21%), HPV6 (262; 2.12%), HPV11 (241; 1.95%), HPV44 (145; 1.17%), HPV43 (63; 0.51%), and HPV42 (48; 0.39%).

Women were divided into different age groups. The highest HPV detection rate was found in age group 41–45 years old (detection rate = 17.89%) (χ2 = 204.77; P < 0.001) (see Table 1); but the highest within-group HPV infection rates were found in the ≤20 (25.62%) and ≥ 61 (24.67%) years old age groups, the lowest within-group HPV infection rate was found in the 31–35 years old age group (15.02%) (see Table 1). The highest mix infection proportions were found in the ≥61 (36.06%) and ≤ 20 (33.63%) years old groups (χ2 = 111.21; P < 0.001), the lowest mix infection proportion was found in the 41–45 (17.42%) years old age group (see Table 2). The highest high risk infection proportions were found in the 26–30 (92.98%), ≥61 (92.68%), and 36–40 (92.16%) years old groups (χ2 = 31.72; P < 0.001), the lowest high risk infection proportion was found in the ≤20 (84.96%) years old age group (see Table 2).

Table 1 Detection rate and positive rate of patients in each age group
Table 2 High risk proportion and mix infection proportion of each age group

The number and detection rate of different HPV types detected per patient were listed in Table 3. At most eight infection was found. High risk and high risk mix infection (1458; 70.85%) was the most common mix HPV infection type with overriding advantage (see Table 3).

Table 3 Percentage of single infection and mixed infections

HPV positive rates of different seasons were analyzed. Months were divided into Spring (March, April and May), Summer (June, July and August), Autumn (September, October and November) and Winter (January, February and December) according to the standard QX/T152–2012 of China Meteorological Information Center (data.cma.cn). We found that positive rate of HPV varied with seasons. The highest and lowest (χ2 = 39.46.; P < 0.001) HPV positive rates were found in Spring (2699/14600; 18.49%) and Autumn (2148/13601; 15.79%), respectively (see Table 4).

Table 4 Positive rates of four seasons

Discussion

This study investigated the HPV distribution characteristics in Guizhou. This is the first HPV distribution study with large sample size in Guizhou. Data presented in this study was useful for HPV detection, prevention and treatment in Guizhou.

HPV16, HPV18, HPV31, HPV58 and HPV52 were the five most common high risk HPV types worldwide; HPV16 and HPV18 account for approximately 70% of cervical cancer cases [6, 7]. While HPV16, HPV52, HPV18, HPV51 and HPV58 were the five most common high risk HPV types in China [6]. In the present study, HPV52, HPV16, HPV58, HPV53 and HPV39 were the five most common high risk HPV types in Guizhou. HPV53, HPV39 and HPV51 were more common than HPV18; HPV53 and HPV51 were rarely reported as the most common types in China recently [8,9,10,11,12,13,14]. No HPV vaccine target HPV53, HPV39 and HPV51 until now. So HPV53, HPV39 and HPV51 should be important targets of high risk HPV prevention by detection. HPV vaccine for HPV53, HPV39 and HPV51 should be developed for Guizhou.

HPV6 and HPV11 were the most common low risk HPV types worldwide and in China [9]. But in the present study, we found that HPV81 was the most common low risk type with overwhelming advantage. And what was worse, no HPV vaccine target HPV81 until now. So low risk HPV prevention key target by detection may should be adjusted from HPV6 and HPV11 to HPV81. HPV81 vaccine should be developed for Guizhou.

High-risk HPV persistent infection is the main reason of cervical cancer [15]. In the present study, we found that the high risk HPV infection proportions were up to 90.70 and 92.98% in all and in 26–30 years old age group HPV positive women, respectively. Proportion of high risk HPV infection in Guizhou was much higher than studies in other recent reports in China [8,9,10,11,12,13,14].

In the present study, we found that the most prevalent HPV infection age was between 41 and 45 years old (detection rate = 17.89%), similar to few recent studies in China [9, 10], but different from most studies in and out of China [8, 11,12,13,14, 16, 17]. The HPV with-in group positive rate, mix infection proportion and high risk infection proportion were all relatively high in age group ≥61. Ideal prevention strategies for Guizhou population should be developed according to the HPV distribution characteristics of each age group in Guizhou, especially for the age groups with highest HPV with-in group positive rates, mix infection proportions and high risk infection proportions.

Whether HPV mix infections increase the risk of disease is not clear. Antigens vary among different HPV types; cross-defense is not effective enough [18]. HPV mix infection may increase the risk of HPV-related diseases. Compared to similar studies, high risk and high risk HPV mix infection in Guizhou was very high [8, 9]. Age groups with higher HPV mix infection in the present study may need more attention in HPV prevention in Guizhou.

In this study, we found that there were statistical differences in HPV positive rates among different seasons, indicating that season factor should also be taken in to consideration in HPV prevention in Guizhou.

Conclusion

HPV52, HPV16, HPV58, HPV53 and HPV39 were the five most common high risk HPV types in Guizhou. HPV81 was the most common low risk type in Guizhou. High risk HPV infection proportion was very high in Guizhou. There were significant differences in HPV distribution among age groups, prevention strategy should be adjusted according to the HPV characteristics of each group. HPV infection rates varied with seasons in Guizhou.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Abbreviations

HPV:

Human papillomavirus

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Acknowledgments

We thank for doctors of obstetrics and gynecology department of Affiliated Hospital of Zunyi Medical University.

Funding

This reseach was funded by Affiliated Hospital of Zunyi Medical University (number 201808) and Innovation and Entrepreneurship Training Program for College Students (number ZKDC2018017 and 20195201909).

Author information

ZC, QL and ZC conceived and designed the study. ZC, QL, QH, HL, HJ, ZC, ZA and QL performed the experiments. ZC and QL analyzed the data. ZC and QL wrote the paper. All authors read and approved the final manuscript.

Correspondence to Zehui Chen.

Ethics declarations

Ethics approval and consent to participate

The study was approved by the Ethics Committee of Affiliated Hospital of Zunyi Medical University, the approval number was ZYFYLS2018(81). Before sample collection, a written informed consent was obtained from all the patients or their guardians, and patient/study subject privacy was carefully protected.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Chen, Z., Li, Q., Huang, Q. et al. Characteristics of human papillomaviruses distribution in Guizhou Province, China. Virol J 16, 123 (2019) doi:10.1186/s12985-019-1239-0

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Keywords

  • Human papillomavirus
  • Season
  • Age group
  • Distribution