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Fig. 1 | Virology Journal

Fig. 1

From: A candidate DNA vaccine encoding a fusion protein of porcine complement C3d-P28 and ORF2 of porcine circovirus type 2 induces cross-protective immunity against PCV2b and PCV2d in pigs

Fig. 1

Construction of the plasmids PVAX1-ORF2 and PVAX1-ORF2-C3d-P28.3 (a) Top: the ORF2 construct that was used as a vaccine insert; Bottom: the ORF2-C3d-P28.3 construct that was used as a vaccine insert. Linkers composed of two repeats of four glycines and a serine [(G4S)2] were fused at the junctures of ORF2 and C3d and between each C3d repeat. b Restriction digest of PVAX1-ORF2 and PVAX1-ORF2-C3d-P28.3. Lane 1: DL2000 marker; lane 2: PVAX1-ORF2 digested with Nhe I/Hind III; lane 3: PVAX1-ORF2-C3d-P28.3 digested with Nhe I/Hind III; lane 4: DL5000 marker

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