Fig. 2
From: Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector
Construction and identification of recombinant phage. a Phage genome was digested by restriction enzyme SwaI. M, λ-Hind III digested molecular marker; 1, genome of T7-Tat; 2, genome of T7-EEB; 3, genome of T7-EEB-Tat. b SDS-PAGE on 12% polyacrylamide gel. M, protein marker; 1, lysate of T7-Tat; 2, purified T7-Tat phage. c Western blotting on nitrocellulose membrane using T7 Tag-HRP monoclonal antibody. M, protein marker; 1, lysate of host BL21; 2, lysate of T7 Select 415-1b; 3, lysate of T7-Tat