Fig. 1
From: Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector
Construction of plasmids and structure of recombinant T7 phage. a Homologous arms were amplified by PCR and cloned into pUC-19 to construct plasmid pUC-L-R. Intact eukaryotic expression box was cloned into pUC-L-R to construct shuttle plasmid pUC-L-EEB-R. b tat gene was artificial synthesized and inserted into T7 selected 415-1b genome to rescue recombinant phage T7-Tat. c Recombinant phage T7-EEB-Tat was constructed which display Tat peptides on surface and contain eukaryotic expression box in the left part of its genome