Fig. 2

Treatment of the RSV infection with different inhibitors. HEp-2 cells were pretreated with the inhibitors in a pre-defined concentration range for 1 h, infected with RSV A2 in the presence of inhibitor for 2 h and incubated with inhibitor for an additional 16 h. Afterwards, cells were fixed, permeabilized, incubated with a pAb goat anti-RSV antibody and stained with AF488 Donkey anti-goat (IgG). Nuclei were visualized with DAPI and analyzed with fluorescence microscopy. RSV positive cells were counted and calculated to the total number of nuclei. Results are expressed as means (±SEM) (n = 3), significant differences compared to the untreated control (0 μM – 0 mM) are indicated by ***p < 0.001