Fig. 1

HSV-1 infection triggers a MAVS-Pex-dependent early induction of viperin. a HEK293 cells were pretreated with or without 50 ng/ml BFA and infected with or without WT HSV-1 at an MOI of 0.1 or 0.5. At 2 h post infection, the supernatants were discarded and replaced with fresh media containing BFA for 18 h. Then, the supernatants were collected and added to Viperin-Luc and pRL-TK transfected cells for DLR analysis. (b and c) HEK293 cells were pretreated with 50 ng/ml BFA and infected with or without WT HSV-1 at an MOI of 0.1 (+) or 0.5 (++). At 2 h post infection, the supernatants were discarded and replaced with fresh media containing BFA. At 18 h post infection, cells were harvested and subjected to RT-PCR (b) and WB analysis with anti-viperin antibody (c). d MAVS-KO and WT MEF cells were pretreated with 100 ng/ml BFA and infected with or without WT HSV-1 at an MOI of 0.1 (+) or 0.5 (++). At 2 h post infection, the supernatants were discarded and replaced with fresh media and the cells were transfected with Vig1-Luc and pRL-TK for 18 h in the presence of BFA and subjected to DLR analysis. The data represent results from one of the triplicate experiments