Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infection

Fig. 2

NDV effectively induces cell death in ciPSCs. Infected BA3NI cells show increasingly severe cytopathic effect from 24 hpi (panel a) to 72 hpi (panels b), which is associated with RFP fluorescence (panels c and d). Non-infected controls at the same time are confluent (panels e and f) and do not show RFP fluorescence (panels g and h). At 72 hpi, the few surviving cells left do not show cytopathic effect (Panel i). In infected cells, fluorescence has the highest intensity at 24 hpi, and subsides at 72 hpi in surviving cells (panel d and j). A representative counting of infected compared to non-infected BA3NI cells is shown in the graph (panel k). D-1, day of cell plating; D0, time of infection; D3, 72 hpi (day 3 post infection). For each experiment, n = 3. Counting values are expressed as mean ± SD. *indicates significant differences between average number of cells at each time point (two-way ANOVA for repeated measurements, Sidak’s test for multiple comparisons, p < 0.05)

Back to article page

Virology Journal

ISSN: 1743-422X