Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene

Table 1 Primers used in this study

Primer name	Nucleotide sequence, 5′-3′	Size(bp)	Primer location^a
PEDV-F	TTTATTCTGTCACGCCAT	197	2,2709–22,726
PEDV-R	AGATTTACAAACACCTATGTTA		22,884–22,905
S1U1-F	GGTAAGTTGCTAGTGCGTAA	1461	20,570–20,589
S1U1-R	CAGGATCATCACAATAAAGAA	1461	22,010–22,030
S1U2-F	TTTCTGGACCATAGCATC	1117	21,939–21,956
S1U2-R	AGCACAATCAACACTAAC	1117	23,038–23,055
ORF3-F	TCCTAGACTTCAACCTTACG	833	24,741–24,760
ORF3-R	GGTGACAAGTGAAGCACAGA	833	25,551–25,570

ISSN: 1743-422X